Matrix metalloproteinase inhibitor RECK expression in canine tumors   总被引：5，自引：0，他引：5  

Takagi S  Kato Y  Asano K  Ohsaki T  Bosnakovski D  Hoshino Y  Okumura M  Kadosawa T  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(8):761-767

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Ventilatory failure and successful management for a dog with severe cervical meningioma     

Asano K  Kadosawa T  Mori T  Miyamoto T  Ueno H  Watanabe K  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(6):599-602

A 12-year-old intact male mongrel dog with a weight of 22 kg was referred with a complaint of progressive tetraparesis. Cervical myelography revealed an intradural-extramedullary mass at the second cervical vertebra. After computed tomography (CT) under general anesthesia, the patient showed dyspnea and cyanosis caused by insufficient movement of the chest wall. Positive pressure ventilation was therefore initiated. Hemilaminectomy and partial mass removal were performed 12 hr after the CT. The mass was histopathologically diagnosed as meningioma. Gradual weaning from the mechanical ventilation lasted for 80 hr after the operation. The patient eventually recovered from the ventilatory failure and the tetraparesis at approximately 6 and 14 days after the operation, respectively.  相似文献   

Preventive effect of hyaluronic acid on the suppression of attachment and migration abilities of bovine chondrocytes by IL-1alpha in vitro     

Kim G  Okumura M  Ishiguro T  Kadosawa T  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):427-430

Attachment and migration of bovine chondrocytes cultured in vitro were significantly suppressed by the addition of interleukin (IL)-1alpha at the concentration of 1 ng/ml or more (p<0.05). The application of hyaluronic acid (HA) at the concentration of 10 micro g/m l or more significantly recovered the attachment of chondrocytes (p<0.05) and the application of HA at 100 micro g/ml concentration recovered the migration of chondrocytes suppressed by IL-1alpha. These results suggest that the application of HA for inflammatory arthropathies or chondrocyte transplantation might be helpful to preserve the properties of chondrocytes and its extracellular matrix against inflammatory conditions.  相似文献   

Development of a pen-site test kit for the rapid diagnosis of H7 highly pathogenic avian influenza   总被引：1，自引：0，他引：1  

Manzoor R  Sakoda Y  Sakabe S  Mochizuki T  Namba Y  Tsuda Y  Kida H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):557-562

As well as H5 highly pathogenic avian influenza viruses (HPAIV), H7 HPAIV strains have caused serious damages in poultry industries worldwide. Cases of bird-to-human transmission of H7 HPAIV have also been reported [11]. On the outbreak of avian influenza, rapid diagnosis is critical not only for the control of HPAI but also for human health. In the present study, a rapid diagnosis kit based on immunochromatography for the detection of H7 hemagglutinin (HA) antigen of influenza A virus was developed using 2 monoclonal antibodies that recognize different epitopes on the H7 HAs. The kit detected each of the tested 15 H7 influenza virus strains and did not react with influenza A viruses of the other subtypes than H7 or other avian viral and bacterial pathogens. The kit detected H7 HA antigen in the swabs and tissue homogenates of the chickens experimentally infected with HPAIV strain A/chicken/Netherlands/2586/03 (H7N7). The results indicate that the present kit is specific and sensitive enough for the diagnosis of HPAI caused by H7 viruses, thus, recommended for the field application as a pen-site test kit.  相似文献   

Seasonal variation of paulownia witches’-broom phytoplasma in paulownia trees and distribution of the disease in the Tohoku district of Japan     

Hitoshi Nakamura  Shigeto Ohgake  Norio Sahashi  Nobuyuki Yoshikawa  Takanori Kubono  Tsuyoshi Takahashi 《Journal of Forest Research》1998,3(1):39-42

Seasonal variation of paulownia witches’-broom (PWB) phytoplasma within different organs (leaves, branch and trunk bark and roots) in paulownia trees was investigated by the amplification of a PWB-specific DNA fragment by the polymerase chain reaction (PCR). In leaf samples, PWB phytoplasma was first detected in June and the incidence gradually increased. On the other hand, the PWB was detected at relatively low incidence in branch bark, trunk bark and roots and the incidence did not change among seasons. A survey of PWB in 27 fields in the Tohoku district of Japan showed that malformed flower buds were observed in paulownia trees in almost all of the fields. PWB-phytoplasma was also detected by PCR from paulownia trees in almost all of the fields in Iwate and Fukushima Prefectures. The frequencies of trees in which phytoplasma was detected by PCR were higher than those in which symptoms were observed. These results indicated that PCR amplification of a PWB-specific DNA fragment is an effective tool for practical diagnose and that PWB is widely distributed in the Tohoku district of Japan.  相似文献   

Enhancement of the biological resistance of wood by phenylboronic acid treatment     

Mustafa Kemal Yalinkiliç  Tsuyoshi Yoshimura  Munezoh Takahashi 《Journal of Wood Science》1998,44(2):152-157

Phenylboronic acid (PBA) was tested in terms of boron leachability from treated wood. In addition, the fungal and termiticidal efficacy of PBA-impregnated sugi (Cryptomeria japonica D. Don) wood was tested against the decay fungi Coriolus versicolor (L. ex Fr.) Quel. and Tyromyces palustris (Berk. et Curt) Murr., representing white-rot and brown-rot fungi, respectively, and the Formosan subterranean termite Coptotermes formosanus Shiraki. Ion chromatography analysis of hot water extracts of treated wood before and after leaching indicated that PBA is considerably resistant to water leaching, and saturation of the treatment solution increased the fixation ratio of boron in wood, whereas boric acid could not remain in wood impregnated even with the saturated solution. Decay test results revealed the excellent bioactive performance of PBA. Wood treated with 0.34% PBA solution was found resistant to both decay fungi, even after running-water leaching for 10 days and treatment with 1.00% PBA completely inactivated the Formosan subterranean termite for the leached specimens. Weight gain levels were 0.18% w/w (0.46kg/m³) and 0.99% w/w (2.49kg/m³) for these concentration levels, respectively, after being leached by running water. Contrary to the general belief that boron is a slow-acting toxicant against termites and unable to prevent mass loss of treated wood, PBA acted rapidly, and the mass loss caused by termites was low.This study was presented in part at the 47th annual meeting of the Japan Wood Research Society, Kochi, April, 1997  相似文献   

Characterization of the Q.Ymym region on wheat chromosome 2D associated with wheat yellow mosaic virus resistance     

Fuminori Kobayashi  Hisayo Kojima  Tsuyoshi Tanaka  Mika Saito  Chikako Kiribuchi-Otobe  Toshiki Nakamura 《Plant Breeding》2020,139(1):93-106

Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat in Japan and China. A single major QTL for WYMV resistance in the Japanese wheat variety 'Yumechikara', designated Q.Ymym, has been mapped on a 43.6 cM linkage block between the two markers Xcfd233 and Xgwm349 on chromosome 2D. We were able to obtain two recombinants within the block, which facilitated reducing the size of the linkage block. The pseudomolecule sequence of 'Chinese Spring' (CS) indicated that the original Q.Ymym region of 43.6 cM corresponded to 68.5 Mb and the narrowed Q.Ymym region represents a size of 27.3 Mb. The sequence features of the Q.Ymym region were unique in comparison with CS sequences, which may have led to the low recombination rate within the block. The Q.Ymym haplotype block was detected in other WYMV-resistant varieties but not in the susceptible varieties used in this study. The unique sequence structure of the Q.Ymym region allowed the development of co-dominant markers for use in marker-assisted selection.  相似文献   

Mid-to-caudal partial hemipelvectomy with limb preservation for ischial tumor in a dog     

Yoshifumi ENDO  Toshikazu SAKAI  Sho FUKUI  Ai HORI  Ryosuke ECHIGO  Satoru MATSUNAGA  Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):218

A 10-year-old spayed female Golden Retriever was referred for hindlimb lameness. A firm mass was palpated over the right caudal pelvis. Computed tomography revealed an osteolytic bone region and an associated periosteal reaction in the ischium, including the acetabulum. The histological diagnosis was sarcoma of unknown origin. A mid-to-caudal partial hemipelvectomy was performed to remove the mass. Femoral head and neck osteotomy was performed to allow hindlimb preservation. Following surgery, the dog regained satisfactory hindlimb use over time and was alive for 821 days with no recurrence or metastatic disease. This report indicates that mid-to-caudal partial hemipelvectomy with femoral head and neck osteotomy is technically feasible and allows for tumor control with preservation of the hindlimb and its function.  相似文献   

Evaluation of the temperature and relative humidity preferences of the western dry-wood termite <Emphasis Type="Italic">Incisitermes minor</Emphasis> (Hagen) using acoustic emission (AE) monitoring     

Yuliati Indrayani  Tsuyoshi Yoshimura  Yoshiyuki Yanase  Yoshihisa Fujii  Yuji Imamura 《Journal of Wood Science》2007,53(1):76-79

Twenty-four different combinations of six temperatures (15°, 20°, 25°, 30°, 35°, and 40°C) and four relative humidity (RH) (60%, 70%, 80%, and 90%) conditions were used for pseudergates of the western dry-wood termite Incisitermes minor (Hagen). The feeding activities of the termites were monitored by the detection of generated acoustic emission (AE) events from feeder wood blocks in a test chamber. Temperature and RH showed independent and interactive significant effects on the feeding activity of I. minor. The optimal temperature and RH conditions for the feeding activities were 35°C and 70%, respectively, and the optimal combinations were 35°C-70% and 35°C-80% with an exceptionally higher feeding activity at the combination condition of 30°C-70%.  相似文献   

Biofilm formation and resistance to bactericides of <Emphasis Type="Italic">Pseudomonas syringae</Emphasis> pv. <Emphasis Type="Italic">theae</Emphasis>     

Tsuyoshi Tomihama  Yatsuka Nishi  Kei Arai 《Journal of General Plant Pathology》2007,73(3):193-196

Biofilm-grown cells of Pseudomonas syringae pv. theae (P.s.theae) wild-type strain K9301 on abiotic surface had remarkable resistance to kasugamycin in comparison to planktonically grown cells; however, the biofilm-grown cells of K9301 had the same sensitivity to copper sulfate. Because both the lesser biofilm-forming strain K9301S3 and enhanced biofilm-forming strain K9301-6 also had remarkable biofilm resistance to kasugamycin just as K9301 did and because epigallocatechin gallate, which enhanced biofilm formation of P.s.theae, had no effect on biofilm resistance to kasugamaycin, the degree of biofilm formation was not correlated with the antibiotic susceptibilities. In addition, K9301 and K9301S3 had less sensitivity to kasugamycin but had high sensitivity to copper sulfate on nonwounded leaf surfaces. These results indicate a possibility that the mechanism of P.s.theae biofilm resistance to bactericide functions on both abiotic and nonwounded leaf surfaces.  相似文献