Spontaneous Nephroblastoma with Lung Metastasis in a Rat     

Yuko Ito  Kohei Matsushita  Takuma Tsuchiya  Yukari Kohara  Tsuyoshi Yoshikawa  Makoto Sato  Keisuke Kitaura  Satoshi Matsumoto 《Journal of toxicologic pathology》2014,27(1):91-95

This report describes a spontaneous nephroblastoma with lung metastasis in a 10-week-old male Crl:CD(SD) rat. Macroscopically, a white mass in the kidney and two white masses in the lung were observed. Histopathologically, the renal mass was located in the cortex of a kidney, and it caused pressure on the surrounding renal parenchyma. Three components could be distinguished in the tumor: blastemal, epithelial (primitive glomerular/tubular structures) and mesenchymal (neoplastic connective tissues) elements. Immunohistochemically, the tumor cells were positive for Wilms tumor 1 protein (WT1) and vimentin. Metastasis was found in the lung. Thus, the case was diagnosed as a nephroblastoma with lung metastasis.  相似文献   

Prognostic Utility of Apoptosis Index,Ki-67 and Survivin Expression in Dogs with Nasal Carcinoma Treated with Orthovoltage Radiation Therapy     

Dah-Renn FU  Daiki KATO  Ai WATABE  Yoshifumi ENDO  Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1505-1512

  相似文献   

Buriti pulp oil did not improve high-fat diet-induced metabolic disorders in c57bl/6 mice     

Leonardo Recena Aydos  Luane Aparecida do Amaral  Ana Cristina Jacobowski  Roberta Serafim de Souza  Eduardo Benedetti Parisotto  Mariana Biava de Menezes  Felipe Francisco Bittencourt Junior  Emely Schuindt Fernandes  Iandara Schettert Silva  Luciane Candeloro Portugal  Cláudio Gonçalves Oliveira  Gustavo Tsuyoshi Senra Masuko  Leandro Fontoura Cavalheiro  Carlos Eduardo Domingues Nazário  Elisvânia Freitas dos Santos  Maria Lígia Rodrigues Macedo 《Journal of animal physiology and animal nutrition》2021,105(2):364-375

Metabolic syndrome (MetS) and obesity are growing in many parts of the world, becoming public health problems. It is proposed that foods with functional properties can assist in the treatment of these diseases. Crude buriti pulp oil (BPO) is a food traditionally consumed by residents in the Pantanal, Cerrado and Brazilian Amazon. It is rich in oleic acid, tocopherols and carotenoids, emerging as a potential functional food. Thus, this study aimed to evaluate the effect of the supplementation of BPO on metabolic disorders caused by a high-fat diet. Four groups of C57BL6 mice were used, a lean group with AIN-93M diet and control oil supplementation, an obese group with a high-fat diet and control oil supplementation, and two obese groups with a high-fat diet and BPO supplementation in the amounts of 50 and 100 mg/kg. BPO worsened the metabolic state caused by the high-fat diet, worsening risk factors associated with MetS, as the abdominal circumference and retroperitoneal fat, serum levels of total cholesterol, uric acid, alanine transaminase, glucose and triglycerides, and renal fat, in addition to changes in glycaemic control and oxidative stress markers. C57BL/6 mice fed with a high-fat diet and supplemented with BPO presented a worsening in metabolic risk factors associated with MetS.  相似文献   

Angiotensin I-converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract   总被引：4，自引：0，他引：4  

Saiga A  Okumura T  Makihara T  Katsuta S  Shimizu T  Yamada R  Nishimura T 《Journal of agricultural and food chemistry》2003,51(6):1741-1745

The blood pressure of spontaneously hypertensive rats (SHRs) decreased after oral administration of an extract prepared from chicken breast muscle, falling maximally to 50 mmHg lower than before. This effect continued for at least 4 h after administration. The peptides possessing hypotensive activity in the chicken extract were examined by measuring the inhibitory activity (IC(50)) against angiotensin I-converting enzyme (ACE). The inhibitory activity of the chicken extract was 1060 mg%, whereas the activity of the extract treated with an Aspergillus protease and gastric proteases (trypsin, chymotrypsin, and intestinal juice) became stronger, reaching 1.1 mg%. Peptides in this hydrolysate of the extract were isolated by HPLC on a reversed-phase column, and their N-terminal sequences were analyzed. Three peptides possessed a common sequence, Gly-X-X-Gly-X-X-Gly-X-X, which was homologous with that of collagen. The peptide Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe showed the strongest inhibitory activity (IC(50) = 42 microM).  相似文献   

C-protein (MyBP-C) isoforms from carp ordinary and dark muscles and muscle type-specific binding to myosin     

Tsuyoshi Okagaki  Ryo Suzuki  Atsushi Ooi 《Fisheries Science》2007,73(3):640-650

ABSTRACT:   C-protein is a myosin-associated protein of vertebrate striated muscle, and its function and properties have been extensively examined. However, there has been no report of C-protein of fish skeletal muscle so far. C-protein was identified in carp skeletal muscle by immunoassay using antibody against chicken C-protein, and the muscle-type specific C-protein was purified from carp ordinary and dark muscles for the first time. Although C-protein could be prepared from crude myosin by the reported procedure, C-protein degraded appreciably during the purification steps. Accordingly, C-protein was selectively extracted from the muscle with 0.15 M K-phosphate buffer (pH 5.8), and purified by ammonium sulfate fractionation, followed by AF-blue chromatography. Myosin free from the accessory proteins was obtained by diethylaminoethyl (DEAE) chromatography and used to assay the binding of C-protein with myosin. Ordinary muscle C-protein bound to ordinary muscle myosin in a saturable manner, but its maximum amount of binding was approximately twice that of dark muscle myosin. Similarly, dark muscle C-protein bound to dark muscle myosin much more than to ordinary muscle myosin. These results suggest that C-protein isoforms specifically bound with myosin isoforms originated from the same type of muscle.  相似文献   

Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer     

Watabe A  Fukumoto S  Komatsu T  Endo Y  Kadosawa T 《Veterinary immunology and immunopathology》2011,142(3-4):189-200

Changes in an individual's immune status are considered major contributing factors towards the morbidity of cancer and mortality of aging. To evaluate age-related changes in the immune status of dogs, the immunophenotypes (CD3, CD4, CD8 and CD21) of peripheral blood lymphocytes were measured in 160 healthy dogs aged from 1 to 17 years, and in 365 dogs with various tumors and at various stages. In healthy dogs, the absolute numbers of white blood cells, lymphocytes, and CD3(+), CD4(+) and CD21(+) lymphocytes decreased significantly with age. The relative percentages of lymphocytes and CD4(+) cells decreased significantly, while CD8(+) cells increased significantly with age. The CD4:CD8 ratio showed a significant age-related decrease. In contrast, dogs with tumors possessed significantly lower absolute numbers and relative percentages of all lymphocyte phenotypes, while the CD4:CD8 ratio was significantly higher than in age-matched controls. The relative percentages of CD3(+) and CD8(+) lymphocytes were significantly lower in dogs with distant metastases compared with dogs without metastases, and the CD4:CD8 ratio increased with advanced stage. These observations illustrate the significant changes in immune status with age and the presence of marked immunological defects in a large-scale study of dogs with advanced tumors.  相似文献   

Gene transcription analysis in lesional skin of canine epitheliotropic cutaneous lymphoma using quantitative real-time RT-PCR     

Chimura N  Kondo N  Shibata S  Kimura T  Mori T  Hoshino Y  Murayama N  Nagata M  Ide K  Nishifuji K  Kamishina H  Maeda S 《Veterinary immunology and immunopathology》2011,144(3-4):329-336

  相似文献   

The hypocholesterolemic activity of transgenic rice seed accumulating lactostatin, a bioactive peptide derived from bovine milk β-lactoglobulin     

Wakasa Y  Tamakoshi C  Ohno T  Hirose S  Goto T  Nagaoka S  Takaiwa F 《Journal of agricultural and food chemistry》2011,59(8):3845-3850

Lactostatin is a novel pentapeptide (IIAEK) derived from bovine milk β-lactoglobulin with greater hypocholesterolemic activity than β-sitosterol, the drug commonly used to treat hypercholesterolemia. We developed transgenic rice expressing lactostatin as a fusion protein with seed storage protein (SSP) glutelins under the control of three different endosperm-specific promoters. Lactostatin accumulated in transgenic rice seed at approximately 1.6 mg/g seeds (dry seeds) without any apparent influence on seed traits such as endogenous SSP expression levels or alterations in the intracellular structures of endosperm cells. Short-term (three day) oral administration of the glutelin fraction containing lactostatin (namely three times of 300 mg/kg body weight/day) extracted from transgenic rice seeds resulted in hypocholesterolemic activity in rats; namely, the serum low-density-lipoprotein (LDL) cholesterol level was significantly reduced accompanied by a significant increase in beneficial serum high-density-lipoprotein (HDL) cholesterol.  相似文献   

Reproductive biology of the dolphin fish Corphaena hippurus on the east coast of Taiwan     

Chuen-Chi Wu  Wei-Cheng Su  Tsuyoshi Kawasaki 《Fisheries Science》2001,67(5):784-793

ABSTRACT: The dolphin fish, Corphaena hippurus Linnaeus, is one of the most important migratory fishes on the east coast of Taiwan. Present results are based on an investigation of 1439 specimens caught using long-line and driftnet, and set net collected monthly between September 1996 and September 1997. The sex ratio of female to male and female combined was 65%, demonstrating a female predominance. Oocytes became mature and transparent when they attained 1.0 mm in diameter. The total number of oocytes per ovary was estimated to range from 2.78 × 10⁵ to 23.48 × 10>⁵, but batch fecundity was noticeably lower than the total number of ovarian eggs, ranging 5.3–32.7% (average 30.1%), and the relative fecundity ranging 10–344/g bodyweight (mean 111/g bodyweight). Minimum body size at sexual maturity was estimated to be 51 cm for both sexes. Dolphin fish spawned throughout the year with reproductive activity peaking in February to March. It is suggested that the dolphin fish has an extended spawning season, during which it lays eggs almost continuously.  相似文献   

Phylogenetic comparison of Leucocytozoon spp. from wild birds of Japan     

Sato Y  Hagihara M  Yamaguchi T  Yukawa M  Murata K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):55-59

Eight species of Japanese birds were found to be infected with Leucocytozoon species using microscopic analysis. We used PCR and sequence analysis of the mitochondrial cytochrome b gene (cyt b) to compare the genetic background among these detected protozoa species. In 20 individuals of 22 samples, a single amplified band was detected from 6 of 8 bird species; 9 Japanese rock ptarmigans (Lagopus mutus japonicus), 4 large-billed crows (Corvus macrorhynchos), 2 carrion crows (C. corone), 2 scops owls (Otus scops), 1 Japanese grosbeak (Eophona personata), and 2 brown-eared bulbuls (Hypsipetes amaurotis), respectively. Phylogenetic analysis based on the partial cyt b sequences revealed that all Leucocytozoon isolates in Japan closely grouped with other Leucocytozoon species previously reported in the literature. Among the Japanese isolates, the phylogenetic tree suggested that L. lovati from the Japanese rock ptarmigan may be basal to the parasites found in other bird species. Our study is the first to identify the molecular relationships among Leucocytozoon parasites in the avifauna of Japan.  相似文献