Identification and characterization of a tospovirus causing chlorotic ringspots on <Emphasis Type="Italic">Phalaenopsis</Emphasis> orchids     

You-Xiu Zheng  Ching-Chung Chen  Chia-Jin Yang  Shyi-Dong Yeh  Fuh-Jyh Jan 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(2):199-209

A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70–100 nm were observed. Inoculation with isolated virus was conducted to confirm that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the first formal report of a tospovirus infecting Phalaenopsis orchids.  相似文献   

Potential threat of a new pathotype of Papaya leaf distortion mosaic virus infecting transgenic papaya resistant to Papaya ringspot virus     

Bau HJ  Kung YJ  Raja JA  Chan SJ  Chen KC  Chen YK  Wu HW  Yeh SD 《Phytopathology》2008,98(7):848-856

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Melon yellow spot virus in watermelon: a first record from Taiwan     

T.-C. Chen  Y.-Y. Lu  Y.-H. Cheng  C.-A. Chang  S.-D. Yeh 《Plant pathology》2008,57(4):765-765

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Construction,expression and characterization of 11 putative flagellar apparatus genes of Aeromonas hydrophila AL09‐73     

H‐Y Yeh  P H Klesius 《Journal of fish diseases》2012,35(11):853-860

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Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque)     

Yeh HY  Klesius PH 《Fish physiology and biochemistry》2012,38(4):1083-1098

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A vascularized preputial free flap for labial reconstruction in a dog          下载免费PDF全文

Chi‐Hsin Liu DVM  MS  Lih‐Seng Yeh DVM  PhD 《Veterinary surgery : VS》2017,46(8):1116-1119

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Adverse effect of the chitinolytic enzyme PjCHI‐1 in transgenic tomato on egg mass production and embryonic development of Meloidogyne incognita     

Y.‐L. Chan  D. Cai  P. W. J. Taylor  M.‐T. Chan  K. W. Yeh 《Plant pathology》2010,59(5):922-930

A novel chitinase gene (PjCHI‐1) isolated from Paecilomyces javanicus, a non‐nematophagous fungus, and driven by a CaMV35S promoter, was delivered into CLN2468D, a heat‐tolerant cultivar of tomato (Solanum lycopersicum). T₁ tomato plants exhibited high endochitinase activity and reduced numbers of eggs and egg masses when infected with the root‐knot nematode (RKN) Meloidogyne incognita. The eggs found in transgenic tomato had lower shell chitin contents than eggs collected from control plants. Egg masses from transgenic plants exhibited higher chitinase activity than those from control plants. Moreover, only 30% of eggs from transgenic plants were able to develop to the multi‐cell/J1 stage, compared with more than 96% from control plants. The present study demonstrated that the expression of the PjCHI‐1 chitinase gene can effectively reduce the production of egg masses and repress the embryonic development of M. incognita, presenting the possibility of a novel agro‐biotechnological strategy for preventing crop damage by RKN.  相似文献   

Quantitative trait loci influencing fruit-related characteristics of tomato grown in high-temperature conditions     

Kuan-Hung Lin  Wei-Lung Yeh  Huei-Mei Chen  Hsiao-Feng Lo 《Euphytica》2010,174(1):119-135

The objective of this study was to identify molecular markers linked to fruit-related traits in the tomato subjected to high temperatures. In total, 160 F₂ plants derived from a cross between a heat-tolerant breeding line, CL5915-93D4-1-0-3 (Solanum esculentum), and a heat-sensitive wild accession, L4422 (S. pimpinellifolium), were grown in a greenhouse. Six traits including fruit number, fruit weight, brix, seed number, fruit setting, and flower number were scored. The distributions of fruit number, fruit set, flower number, and seed number were skewed towards heat susceptibility which is known to be characteristic of L4422. Polymorphic bands were generated by PCR-derived methods of RAPD, ISSR and AFLP Polymorphism, the segregation ratio, and distribution over the genome of the above 3 markers were compared. Ten linkage groups, ranging 20.6–151.6 cM in size, were constructed with 62 informative markers spanning a total of 776.3 cM. Fruit-related quantitative trait loci (QTLs) were non-randomly distributed in the tomato genome. For the 6 traits investigated, 21 QTLs were dispersed on linkage groups 2–5. The genetic effects of the various QTLs were differently exhibited, in our study we have respectively found from 10.5% to 30.2% of the variation explained by the QTL for flower number (FRN4) and brix (BX2). Thirteen QTL-mapped markers were unique to 1 trait, and 4 markers were linked to more than 1 trait. Among them, QTLs linked to the I868-470 marker had effects on fruit weight and brix, and a significant positive correlation between these 2 traits was noted (r = 0.35, P < 0.05). Thus, the I868-470 marker may have the potential for simultaneous selection of high fruit weight and brix. These markers also allowed us to align genome linkage maps across distantly related species and to reveal the co-localization between these QTLs and major genes.  相似文献   

Heat tolerance in English ivy as measured by an electrolyte leakage technique     

D. M. Yeh  P.Y. Hsu 《The Journal of Horticultural Science and Biotechnology》2013,88(2):298-302

Summary

Identification of heat-tolerant English ivy (Hedera helix L.) genotypes for commercial production in hot areas is desirable. The extent to which electrolyte leakage from English ivy leaf discs, measured using a test for cell membrane thermostability (CMT), could be related to the reduction to shoot dry weight induced by heat in the greenhousegrown plants was determined. A curved relationship existed between the relative injury (RI) value occurring in leaf tissue discs of English ivy cultivars and treatment temperature. A single temperature treatment at 50°C resulted in injury values near the midpoint of the response curve and showed the greatest sensitivity in detecting genotypic differences in heat tolerance. The cultivars with a high RI value are those with the lesser CMT and more reduction in shoot dry weights by high day/night temperature at 35/30°C.  相似文献   

花生褐斑病菌产孢条件的研究     

黎穗临  叶维霖 《中国油料作物学报》1990,(2)

花生褐斑病菌(Cercospora arachidicola Hori)在花生秆汁培养基上的产孢量优于前人推荐的3种培养基。培养过程中,不同光照处理对病菌产孢不敏感;不同温度处理产孢量有显著差异。  相似文献