A comparative study of the effects of intramuscular administration of gonadorelin,mating and intrauterine infusion of either raw seminal plasma or seminal plasma purified β‐NGF on luteal development in llamas          下载免费PDF全文

M Silva  F Urra  C Ulloa‐Leal  MH Ratto 《Reproduction in domestic animals》2017,52(4):625-631

The aim of this study was to compare the effect of the intramuscular administration of 50 μg of gonadorelin acetate versus natural mating, intrauterine infusion (i.u.) of a physiological relevant dose of either raw llama seminal plasma (SP) or purified beta‐nerve growth factor from seminal origin (spβ‐NGF) on ovulation rate and corpus luteum (CL) development and function in llamas. Females with a follicle (≥8 mm) were assigned to groups: (i) i.m. administration of 50 μg of gonadorelin acetate (GnRH; positive control; n = 4); (ii) single mating (mating; n = 6); (iii) i.u. infusion of 4 ml of llama SP (SP; n = 4); or (iv) i.u. infusion of 10 mg of spβ‐NGF contained in 4 ml of PBS (phosphate‐buffered saline) (spβ‐NGF; n = 6). Ovaries were examined by power Doppler ultrasonography at 0, 1, 3, 6, 12 and 24 hr after treatment to determine preovulatory follicle vascularization area (VA), and additionally every 12 hr until Day 2 (Day of treatment = Day 0) to determine ovulation. Afterwards, ovaries were examined every other day until Day 8 to evaluate CL diameter and VA. Blood samples were collected on Days 0, 2, 4, 6 and 8 to determine plasma progesterone (P4) concentration. Ovulation rate did not differ (p = .7) among groups, but treatment affected (p < .0001) preovulatory follicle VA. Neither treatment administration nor treatment by time interaction affected (p ≥ .4) CL diameter, VA and plasma P4 concentration. Mating tended (p = .08) to increase CL VA when compared to the seminal plasma group by Day 8. Intrauterine administration of seminal plasma or spβ‐NGF does not increase CL size and function when compared to i.m. GnRH treatment, suggesting that the administration route of spβ‐NGF influences its luteotrophic effect in llamas.  相似文献   

Effect of N‐acetyl‐L‐cysteine Supplementation in Semen Extenders on Semen Quality and Reactive Oxygen Species of Chilled Canine Spermatozoa     

AJ Michael  C Alexopoulos  EA Pontiki  DJ Hadjipavlou‐Litina  P Saratsis  HN Ververidis  CM Boscos 《Reproduction in domestic animals》2010,45(2):201-207

The objective of this study was to evaluate the quality of chilled dog semen processed with extenders containing various concentrations of N‐acetyl‐L‐cysteine (NAC). Ejaculates from five dogs were collected, pooled and evaluated for concentration, motility, rapid steady forward movement (RSF‐movement), viability, acrosomal integrity and by the hypo‐osmotic swelling test (HOST). In addition, superoxide anion (O₂^‐?) production, hydroxyl radicals (OH^?) and total reactive oxygen species (tROS) were determined. The pool was divided into five aliquots, which were diluted to a final concentration of 66.66 × 10⁶ spermatozoa/ml with Tris‐glucose‐egg yolk extender containing one of the following concentrations of NAC (0, 0.5, 1, 2.5 or 5 mm ). The semen aliquots were chilled and preserved at 4°C. Semen quality was evaluated after rewarming at 72 h. Sperm motility was significantly higher with the 0.5 mm concentration compared with the control group (p = 0.001). Rapid steady forward movement was higher with the 0.5 and 1 mm concentrations compared with the control and 5 mm group (p < 0.001). Viability and HOST percentages were not significantly altered. Compared with the control, the 5 mm concentration showed significantly reduced percentages of spermatozoa with normal acrosomes (p = 0.049). None of the ROS values at 72 h were significantly affected by the presence of NAC in semen extenders, although all NAC concentrations showed lower O₂^‐? and OH^? values compared with the control. Only the concentrations of 1 and 5 mm inhibited the significant increase of tROS values after 72 h, compared with the fresh semen value. In conclusion, NAC supplementation of semen extenders is beneficial to semen motility of canine spermatozoa during chilling with the 0.5 mm concentration being the most effective, although no significant ROS inhibition was observed at 72 h.  相似文献   

Gene action analysis by inheritance and quantitative trait loci mapping of resistance to root‐knot nematodes in cotton     

M. Ulloa  C. Wang  P. A. Roberts 《Plant Breeding》2010,129(5):541-550

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Tolerance of selected weed species to broadcast flaming at different growth stages     

Santiago M. Ulloa  Avishek DattaStevan Z. Knezevic 《Crop Protection》2010

Organic producers rank weeds as the most important pests that limit their crop production. In order to optimize the use of propane flaming as a weed control tool, the objective of this study was to test tolerance of selected weed species to broadcast flaming performed at different growth stages. Six annual species, including one grass [barnyardgrass (Echinochloa crus-galli)] and five broadleaves [field bindweed (Convolvulus arvensis), kochia (Kochia scoparia), ivyleaf morning glory (Ipomoea hederacea), velvetleaf (Abutilon theophrasti) and Venice mallow (Hibiscus trionum)] were flamed at three growth stages with six doses of propane. The propane doses applied were 0, 12, 31, 50, 68 and 87 kg/ha. Flaming treatments were applied utilizing a custom built flamer mounted on a four-wheeler moving at a constant speed of 6.4 km/h. Species response to propane were described by log-logistic models based on visual injury ratings and dry matter (DM) for each weed species. Overall response to flaming varied among species, growth stage and propane dose. Broadleaf weeds were more susceptible to flaming than the grass regardless of the growth stage. A dose of 76 kg/ha of propane was needed to obtain 90% DM reduction for 7-leaf (L) barnyardgrass compared to much lower doses of 40, 49, 55, 56 and 51 kg/ha propane for 8-L bindweed, 6-L kochia, 10-L morning glory, 7-L velvetleaf and 5-L Venice mallow, respectively. Moreover, the tolerance of both grassy and broadleaf weed species to broadcast flaming increased with increase in plant size. A 90% DM reduction in velvetleaf was obtained with 42, 56 and 102 kg/ha of propane for 5-L, 7-L and 16-L stages, respectively. The tested broadleaf weed species were effectively controlled (90% DM reduction) with propane dose of 30–60 kg/ha when flamed at early growth stages (3-L to 14-L), while the same dose of propane provided only 80% DM reduction in barnyardgrass when flamed at vegetative stages (4-L to 7-L). It was not possible to obtain 90% DM reduction in barnyardgrass when flamed at flowering stage with the propane doses tested in this experiment.  相似文献   

Popcorn (Zea mays L. var. everta) yield and yield components as influenced by the timing of broadcast flaming     

Santiago M. Ulloa  Avishek Datta  Sidnei D. Cavalieri  Mario Lesnik  Stevan Z. Knezevic 《Crop Protection》2010

Farmers are interested in producing popcorn under organic production systems and propane flaming could be a significant component of an integrated weed management program. The objective of this study was to collect baseline information on popcorn tolerance to broadcast flaming as influenced by propane dose and crop growth stage at the time of flaming. Field experiments were conducted at the Haskell Agricultural Laboratory of the University of Nebraska, Concord, NE in 2008 and 2009 using five propane doses (0, 13, 24, 44 and 85 kg ha⁻¹) applied at the 2-leaf, 5-leaf and 7-leaf growth stages. Propane was applied using a custom-built research flamer driven at a constant speed of 6.4 km h⁻¹. Crop response to propane dose was described by log-logistic models on the basis of visual estimates of crop injury, yield components (plants m⁻², ears plant⁻¹, kernels cob⁻¹ and 100-kernel weight) and grain yield. Popcorn response to flaming was influenced by the crop growth stage and propane dose. Based on various parameters evaluated, popcorn flamed at the 5-leaf showed the highest tolerance while the 2-leaf was the most susceptible stage. The maximum yield reductions were 45%, 9% and 16% for the 2-leaf, 5-leaf and 7-leaf stages, respectively. In addition, propane doses that resulted in a 5% yield loss were 23 kg ha⁻¹ for the 2-leaf and 7-leaf and 30 kg ha⁻¹ for the 5-leaf stage. Flaming has a potential to be used effectively in organic popcorn production if properly used.  相似文献   

The Australian Ark, A history of domesticated animals in Australia     

CM Bunn 《Australian veterinary journal》1999,77(10):666-666

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Effect of dietary protein‐to‐energy ratio on reproduction in female redclaw crayfish Cherax quadricarinatus     

Hervey Rodríguez‐González  Humberto Villarreal  Alfredo Hernández‐Llamas  Manuel García‐Ulloa  Celia Vázquez‐Boucard  Vania Serrano‐Pinto 《Aquaculture Research》2011,42(12):1840-1847

The effect of eight diets with different protein‐to‐energy ratios (P–E ratio=10, 11, 14, 16, 18, 20, 22, 24 mg kJ^?1) on reproduction of female redclaw crayfish Cherax quadricarinatus was tested under laboratory conditions. After 75 days, there was a significant effect of the P–E ratio on final weight, spawning females and the number of juveniles per female. Using the broken‐line and quadratic equations, optimal values for the P–E ratio were: 16.0, 17.1 and 16.0 mg kJ^?1. The P–E ratio significantly affected protein and carbohydrate content and energy in the hepatopancreas. The broken‐line model indicated that the respective recommended values of the P–E ratio for these parameters were: 21.8, 16.7 and 18.6 mg kJ^?1. The concentration of carbohydrates and energy in muscle tended to significantly diminish as the P–E ratio increased. From broken‐line regression analysis, the recommended P–E ratio was 16.6 and 20.2 mg kJ^?1 respectively. No significant differences were found in survival, fecundity, vitellin content in eggs, protein and lipid content in muscle and lipids in the hepatopancreas. We concluded that, overall, a P–E ratio of 18±2 mg kJ^?1 is optimal for reproductive activity of female redclaw crayfish.  相似文献   

Effect of mitochondrial uncoupling and glycolysis inhibition on ram sperm functionality          下载免费PDF全文

JDA Losano  DSR Angrimani  A Dalmazzo  BR Rui  MM Brito  CM Mendes  GKV Kawai  CI Vannucchi  MEOA Assumpção  VH Barnabe  M Nichi 《Reproduction in domestic animals》2017,52(2):289-297

Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram sperm in response to mitochondrial uncoupling and glycolysis inhibition aiming to assess the importance of each pathway for sperm functionality. Statistical analysis was performed by the SAS System for Windows, using the General Linear Model Procedure. Data were tested for residue normality and variance homogeneity. A p < .05 was considered significant. Groups treated with the mitochondrial uncoupler Carbonyl cyanide 3 chlorophenylhydrazone (CCCP) showed a decrease in the percentage of cells with low mitochondrial activity and high mitochondrial membrane potential. We also observed that the highest CCCP concentration promotes a decrease in sperm susceptibility to lipid peroxidation. Regardless the lack of effect of CCCP on total motility, this substance induced significant alterations on sperm kinetics. Besides the interference of CCCP on spermatic movement patterns, it was also possible to observe such an effect in samples treated with the inhibitor of glycolysis (2‐deoxy‐d ‐glucose, DOG). Furthermore, treatment with DOG also led to a dose‐dependent increase in sperm susceptibility to lipid peroxidation. Based on our results, we suggest that the glycolysis appears to be as important as oxidative phosphorylation for ovine sperm kinetics as this mechanism is capable of maintaining full motility when most of the cells have a low mitochondrial membrane potential. Furthermore, we found that changes in the glycolytic pathway trough glycolysis inhibition are likely involved in mitochondrial dysfunction and sperm oxidative unbalance.  相似文献   

Treatment of virulent footrot with lincomycin and spectinomycin   总被引：1，自引：0，他引：1  

CM VENNING  MA CURTIS  JR EGERTON 《Australian veterinary journal》1990,67(7):258-260

A mixture of lincomycin and spectinomycin was investigated as a treatment for footrot in sheep. In a controlled clinical trial 92.5% of acute and chronic cases of virulent footrot were cured following a single intramuscular injection of a mixture containing 50 mg lincomycin and 100 mg spectinomycin/ml at a dose rate of 1 ml/10 kg bodyweight. No improvement in clinical response was observed in groups of sheep treated on 3 successive days with this dose rate nor in another group treated once at a dose rate 1 ml/3.3 kg bodyweight. Cure effectiveness of each of the 3 treatment groups relative to untreated controls was 89%, 95% and 95%. Efficacy of lincomycin/spectinomycin was compared with that of penicillin/streptomycin in the treatment of footrot on 2 farms in south western New South Wales. Assessments made 14 to 17 d after treatment showed that on one farm all 122 ewes treated with lincomycin/spectinomycin had recovered while 170 of 175 ewes treated with penicillin/streptomycin recovered in the same period. On the second farm 87 of 90 ewes treated with lincomycin/spectinomycin recovered, compared with 184 of 190 sheep in the same flock treated with penicillin/streptomycin. Supportive footbathing did not seem to improve the clinical response in either treatment group and the paring done was sufficient only to establish diagnosis and to remove grossly overgrown horn.  相似文献   

Aspergillosis in wild geese (Chlo?phaga poliocephala scl., 1857) in Chile     

J Ulloa  V Cubillos  M I Montecinos  A Alberdi 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1987,34(1):30-35

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