Classification approaches for sorting maize (Zea mays subsp. mays) haploids using single-kernel near-infrared spectroscopy     

Jeffery L. Gustin  Ursula K. Frei  John Baier  Paul Armstrong  Thomas Lübberstedt  A. Mark Settles 《Plant Breeding》2020,139(6):1103-1112

Doubled haploids (DHs) are an important breeding tool for creating maize inbred lines. One bottleneck in the DH process is the manual separation of haploids from among the much larger pool of hybrid siblings in a haploid induction cross. Here, we demonstrate the ability of single-kernel near-infrared reflectance spectroscopy (skNIR) to identify haploid kernels. The skNIR is a high-throughput device that acquires an NIR spectrum to predict individual kernel traits. We collected skNIR data from haploid and hybrid kernels in 15 haploid induction crosses and found significant differences in multiple traits such as percent oil, seed weight, or volume, within each cross. The two kernel classes were separated by their NIR profile using Partial Least Squares Linear Discriminant Analysis (PLS-LDA). A general classification model, in which all induction crosses were used in the discrimination model, and a specific model, in which only kernels within a specific induction cross, were compared. Specific models outperformed the general model and were able to enrich a haploid selection pool to above 50% haploids. Applications for the instrument are discussed.  相似文献   

Phenolic compounds in raw and cooked rice (Oryza sativa L.) and their inhibitory effect on the activity of angiotensin I-converting enzyme     

Isabel Louro Massaretto  Márcio Fernando Madureira Alves  Nádia Valéria Mussi de Mira  Adriana Karaoglanovic Carmona  Ursula Maria Lanfer Marquez 《Journal of Cereal Science》2011,54(2):236-240

Whole rice has been widely studied due to the abundance of bioactive compounds in its pericarp. Some of the beneficial effects of these compounds on human health have been attributed to their antioxidant and other biological activities, such as enzyme inhibition. In this work, we evaluated the contents of total, soluble and insoluble phenolic compounds of 6 red and 10 non-pigmented genotypes of whole rice as well as their inhibitory effect on the activity of angiotensin I-converting enzyme (ACE). The effects of cooking on phenolics and their inhibitory activities were also investigated. Red genotypes showed high content of phenolics, mainly soluble compounds, at an average of 409.7 mg ferulic acid eq./100 g, whereas overall lower average levels (99.4 mg ferulic acid eq./100 g) at an approximate soluble/insoluble compound ratio of 1:1 were observed in non-pigmented rice. Pigmented rice displayed a greater inhibitory effect on ACE than non-pigmented rice. In fact, a significant correlation between the content of soluble phenolics and ACE inhibition was observed (r = 0.8985, p < 0.05). In addition to significantly reducing the levels of total phenolics and ACE inhibition, cooking altered the soluble/insoluble compound ratio, especially among red rice genotypes.  相似文献   

Natural Bagaza virus infection in game birds in southern Spain     

Gamino V  Gutierrez-Guzman AV  Fernández de Mera IG  Ortíz JA  Durán-Martin M  De la Fuente J  Gortazar C  Höfle U 《Veterinary research》2012,43(1):65

ABSTRACT: In late summer 2010 a mosquito born flavivirus not previously reported in Europe called Bagaza virus (BAGV) caused high mortality in red-legged partridges (Alectoris rufa) and ring-necked pheasants (Phasianus colchicus). We studied clinical findings, lesions and viral antigen distribution in naturally BAGV infected game birds in order to understand the apparently higher impact on red-legged partridges. The disease induced neurologic signs in the two galliform species and, to a lesser extent, in common wood pigeons (Columba palumbus). In red-legged partridges infection by BAGV caused severe haemosiderosis in the liver and spleen that was absent in pheasants and less evident in common wood pigeons. Also, BAGV antigen was present in vascular endothelium in multiple organs in red-legged partridges, and in the spleen in common wood pigeons, while in ring-necked pheasants it was only detected in neurons and glial cells in the brain. These findings indicate tropism of BAGV for endothelial cells and a severe haemolytic process in red-legged partridges in addition to the central nervous lesions that were found in all three species.  相似文献   

Follow-up investigations on different courses of natural avian bornavirus infections in psittacines     

Heffels-Redmann U  Enderlein D  Herzog S  Piepenbring A  Bürkle M  Neumann D  Herden C  Lierz M 《Avian diseases》2012,56(1):153-159

  相似文献   

Subcutaneous extraskeletal osteosarcoma in a metatarsal footpad in a cat          下载免费PDF全文

Ramón Almela  Wolf von Bomhard  Agustina Ansón  Ursula Mayer 《Veterinary dermatology》2017,28(5):524-e129

  相似文献   

Die Abhängigkeit des Hemmhofdurchmessers von der Antibiotikakonzentration (Dosis-Wirkungs-Funktion)     

H. J. Langner  H. Weiss  Ursula Teufel 《Zoonoses and public health》1973,20(6):435-453

  相似文献   

Action of the microbial metabolite and chitin synthesis inhibitor nikkomycin on the mite Tetranychus urticae; an electron microscope study     

Ursula Mothes  Karl-August Seitz 《Pest management science》1982,13(4):426-441

Chitinous structures of Tetranychus urticae K., after treatment with the microbial metabolite nikkomycin, were examined using an electron microscope. The results were compared with published information on the mechanisms of action of this antibiotic. Application of the drug resulted in an inhibition of moulting, due to a deficient or abnormal differentiation of the procuticle during ecdysis. Damage to the procuticle caused disorders in the muscle attachments, preventing the chrysalis stages leaving their exuviae after treatment with the metabolite. Application of nikkomycin to an adult female caused plug-like accumulations below the procuticle of the prosoma and cranial opisthosoma, as well as inside the hypodermis. The egg shell was another site of action of nikkomycin; depending on when drug treatment began, an abnormally thickened or incomplete plaque-like egg shell was produced. The deposition of affected eggs was strongly inhibited, if they were laid at all. The electron micrographs supported the suggestion that a competitive inhibition of chitin synthetase results in inhibition of cuticle synthesis. However, such a mechanism of action cannot explain the results on egg shell damage after nikkomycin treatment, which indicates some other influences of the antibiotic.  相似文献   

Effects of topical 2% dorzolamide hydrochloride alone and in combination with 0.5% timolol maleate on intraocular pressure in normal feline eyes     

Ursula M. Dietrich  Matthew J. Chandler  Tanya Cooper  Anand Vidyashankar†  Guorong Chen† 《Veterinary ophthalmology》2007,10(S1):95-100

  相似文献   

Osteoclast density is not increased in bone adjacent to radiolucencies (cysts) in juvenile equine medial femoral condyles     

Rosalie Fortin-Trahan  Thibaut Lemirre  Elizabeth M. Santschi  Jennifer G. Janes  Hélène Richard  Ursula Fogarty  Guy Beauchamp  Christiane A. Girard  Sheila Laverty 《Equine veterinary journal》2022,54(5):989-998

  相似文献   

Biblographie der Bestimmungstabellen europäischer Insekten (1880 bis 1963). Teil II: Hymenopteren     

Ursula Göllner-Scheiding 《Journal of pest science》1969,42(7):111-112

Ohne Zusammenfassung  相似文献