Diseases caused by Pythium insidiosum in sheep and goats: a review     

Priscila M. S. do Carmo  Francisco A. Uzal  Franklin Riet-Correa 《Journal of veterinary diagnostic investigation》2021,33(1):20

Pythiosis is characterized most commonly by ulcerative dermatitis, mainly in the limbs of sheep and occasionally of goats. In sheep, Pythium insidiosum is also responsible for necrotizing rhinitis characterized by marked enlargement and deformity of the nasal region, severe respiratory difficulty, and bloody nasal discharge. A third form of pythiosis in sheep affects the digestive tract, involving ulceration of the esophagus, forestomachs, and abomasum. Pythiosis in sheep and goats has been reported only in Brazil where it occurs mainly in the semiarid region of the country, when animals congregate and stay for longer periods of time within or around water reservoirs. However, it has been reported as well in areas of humid environments, such as the Pantanal of Mato Grosso and in the Brazilian Cerrado. The diagnosis of the different presentations of pythiosis is based on gross and microscopic findings, coupled with detection of the agent by immunohistochemical, molecular, and/or culture-based methods.  相似文献   

Pathology of cryptosporidiosis in raccoons: case series and retrospective analysis, 1990–2019     

Viviana Gonzalez-Astudillo  Matthew F. Sheley  Francisco A. Uzal  Mauricio A. Navarro 《Journal of veterinary diagnostic investigation》2021,33(4):721

Cryptosporidiosis is an intestinal protozoal disease of public health importance caused by Cryptosporidium spp. Despite the high synanthropism of raccoons, studies describing the pathology of Cryptosporidium spp. infections in this species are lacking. Therefore, we characterized the pathology of cryptosporidiosis in 2 juvenile raccoons. In addition, we conducted a retrospective search of the database of the California Animal Health and Food Safety laboratory for 1990–2019 and found 6 additional cases of cryptosporidiosis in raccoons. Sequencing of cryptosporidia was performed in one autopsied raccoon, and PCR on formalin-fixed, paraffin-embedded tissues in archived cases. The Cryptosporidium skunk genotype (CSkG), a strain of zoonotic relevance, was detected in 6 of 8 cases (75%). Frequently, cryptosporidiosis was associated with enteritis, eosinophilic infiltrates, villus atrophy or blunting and/or fusion, and crypt abscesses or necrosis. In 7 of the 8 cases, there was confirmed concurrent coinfection with canine distemper virus; 1 case was coinfected with canine parvovirus. Although crypt necrosis is considered a classic lesion of canine parvoviral infection in mesocarnivores and not a hallmark of cryptosporidiosis, results suggest that canine distemper virus is capable of mimicking such lesions in combination with cryptosporidia and immunosuppression.  相似文献   

Enrichment of Y‐Chromosome‐Bearing Bull Spermatozoa by Swim‐up Through a Column     

A Azizeddin  FA Ashkar  WA King  T Revay 《Reproduction in domestic animals》2014,49(1):e1-e4

With the advancement of assisted reproductive biotechnologies, preselecting the sex of offspring has become an important goal for cattle and other livestock breeding as well as for research. The aim of this study was to investigate the feasibility of producing enriched pools of X‐ or Y‐chromosome‐bearing sperm by vertical swim‐up through a long, narrow column. Sperm recovered from the top portion of the column was predominantly Y‐bearing (60%, p < 0.05), which were capable of fertilizing matured oocytes and produce significantly more male embryos compared with standard swim‐up protocol.  相似文献   

安徽特色农业可持续发展研究     

叶良均  法剑 《安徽农业大学学报》2014,(3):1-8；32

当前安徽特色农业呈不断发展趋势：专业化和产业化不断增强、基础设施逐步改善、科技扶持力度不断增强、农业旅游等功能有所拓展。但是也存在优良品种保护和开发力度不够,生产技术水平相对较低,基层农技推广和农村实用人才难以满足需求,基础设施建设投入不足,主产区生态环境保护不够,产业化有待推进,休闲、观光旅游功能拓展缓慢等问题。建议从五个方面推进安徽特色农业可持续发展：加强基层农业科技人才开发;推进特色农业产业化发展;加强优良品种开发与保护;完善农业基础设施建设;保护生态环境,发展农业旅游。  相似文献   

Resistance of Ovine,Caprine and Bovine Endothelial Cells to Clostridium perfringens Type D Epsilon Toxin In Vitro     

Uzal  F.A.  Rolfe  B.E.  Smith  N.J.  Thomas  A.C.  Kelly  W.R. 《Veterinary research communications》1999,23(5):275-284

Ovine, caprine and bovine endothelial cells were grown in vitro and challenged with Clostridium perfringens type D epsilon toxin to compare their susceptibility to this toxin. Madin Darby canine kidney (MDCK) cells, which are known to be susceptible to epsilon toxin, were used as a positive control. No morphological alterations were observed in any of the endothelial cell cultures tested, even after challenging with doses as high as 1200 MLD⁵⁰/ml of epsilon toxin. MDCK cells showed contour rounding and nuclear condensation as early as 30 min after exposure to 100 MLD⁵⁰/ml of epsilon toxin and after 60 min of exposure to 12.5 MLD⁵⁰/ml of the same toxin. All the MDCK cells were dead after 3 h of exposure to all concentrations of epsilon toxin. The results indicate that ovine, caprine and bovine endothelial cells are not morphologically responsive to the action of epsilon toxin in vitro.  相似文献   

优质杂交粳稻新组合申优 27 的选育     

程灿  曹黎明  周继华  储黄伟  牛付安  孙滨  罗忠永  归连发 《中国种业》2020,(4):61-63

对优质杂交粳稻新组合申优27的选育经过、产量表现、主要特征特性、栽培和制种技术进行了介绍。建立了杂交粳稻"双亲双优、抗性互补"育种技术,有效解决了优质和抗性的协调统一问题。  相似文献