P‐20
Clinical,cytopathological and histopathological evaluation of sporotrichosis in experimentally infected cats     

M. R. Farias  S. R. V. S. Franco  V. E. Fabris 《Veterinary dermatology》2004,15(Z1):47-47

The purpose of this study was to evaluate the clinicopathological aspects of experimental sporotrichosis in cats and compare the sensitivity of cytopathology, histopathology and culture as diagnostic tools in different phases of the infection. Twenty adult, mixed‐breed cats (10 males and 10 females) were inoculated subcutaneously with 10⁶ fungal microorganisms. Clinical examination was performed weekly. Cytopathologic, histopathologic and culture examinations were performed at 15, 30 and 60 days postinoculation. Culture of multiple organs was performed after euthanasia at 30 (10 cats) and 60 (10 cats) days postinoculation. Friedman parametric and nonparametric statistical analysis were applied to the results. The nodular, tumoral and necrotic lesionsprogressed significantly until day 30 postinoculation, and partial spontaneous regression occurred at day 60, particularly in males. An intense inflammatory pyogranulatomous and lymphocytic infiltrate with rare giant cells and sparse fibrosis associated with numerous, pleomorphic, intra‐ and extracellular fungal cells were observed on day 30. These findings gradually decreased by day 60. Despite the inflammatory granuloma associated with feline sporotrichosis, a tendency for dissemination was observed, with fungal isolation in the lymph nodes, spleen and liver at the 30 and 60 days postinoculation. No significant differences were observed between cytopathology, histopathology and fungal culture during the different phases of the disease. Therefore, cytological examination was considered a simple, rapid and inexpensive diagnostic method at all stages of this disease. Funding: Self‐funded.  相似文献   

Adrenergic and peptidergic innervation of the trachealis muscle in the normal horse: a preliminary report     

I.M. Sonea  R.M. Bowker  R.V. Broadstone  N.E. Robinson 《Research in veterinary science》1993,54(3):361

The tone of respiratory smooth muscle is largely determined by the input from autonomic nerves. The distribution of adrenergic and selected nonadrenergic, non-cholinergic (NANC) nerves in the normal equine trachealis muscle was investigated using immunohistochemistry. The smooth muscle of the trachealis was found to contain numerous nerves immunoreactive for an enzymatic marker of adrenergic nerves, as well as many nerves immunoreactive for a putative NANC neurotransmitter, peptide histidine isoleucine, a potent bronchodilator. The tissues surrounding the respiratory smooth muscle contained numerous nerves immunoreactive for the neuropeptides substance P and calcitonin gene-related peptide, which can cause marked vasodilation and bronchoconstriction. The complex innervation of the equine trachea should be kept in mind when interpreting the results of physiological experiments.  相似文献   

Distribution of calcium in peat deposits of the central part of Western Siberia     

V. S. Arkhipov  V. K. Bernatonis 《Eurasian Soil Science》2006,39(3):261-269

Distribution patterns of calcium in peat deposits of seventeen bogs in the middle and southern taiga of Western Siberia have been studied. Four types of Ca distribution patterns related to the genesis of peat deposits are specified. Up to three Ca-enriched peat layers are distinguished in thick deposits. This Ca distribution pattern is typical of different peat deposits formed under various conditions. It is shown that Ca accumulation in particular layers of peat deposits is related to the warm stages of the Holocene.  相似文献   

Teratological examination of the insecticide methyl-parathion (Wofatox 50 EC) on pheasant embryos. 2. Biochemical study.     

L Várnagy 《Acta veterinaria Hungarica》1992,40(3):203-206

Fertile pheasant's eggs were treated with the insecticide Wofatox 50 EC (50% methyl-parathion) by injection technique on day 12 of the hatching period. Treatment consisted of inoculation of 0.1 ml of different concentrations of the insecticide into the air space of embryonated eggs. The following dose levels were employed: 0.00, 1.35, 13.5 and 135.0 mg/kg egg of active ingredient. Biochemical changes in the plasma were evaluated by micro (photometric) methods which rendered possible the determination of several blood plasma variables of the embryos. At the highest dose level applied, serum alkaline phosphatase (SAP) enzyme activity and inorganic P concentration of the treated embryos showed statistically differences (reduction) as compared to the control data. Macroscopic alterations were detected at necropsy.  相似文献   

Genetic analysis of bovine respiratory disease in beef calves during the first year of life.     

N E Muggli-Cockett  L V Cundiff  K E Gregory 《Journal of animal science》1992,70(7):2013-2019

The prevalence of bovine respiratory disease (BRD) was assessed in a population of 10,142 beef calves representing nine pure breeds and three composite populations born in 1983 through 1988. Twenty-four percent of the calves experienced at least one episode of respiratory disease during the 1st yr of life; frequencies over the six birth years ranged from 14 to 38%. The timing of respiratory disease outbreaks differed among birth years; in 4 of the 6 yr, more illness occurred in the pasture before weaning than in the feedlot after weaning. Frequencies of BRD during preweaning and postweaning periods were analyzed separately. Pure breeds and composite populations within a single preweaning location differed in frequency of illness during the preweaning period. However, not all possible breed comparisons could be made because preweaning location differed for the breed groups, and preweaning location had a significant effect on the frequency of respiratory disease in the preweaning period. The preweaning location effect did not carry through to the postweaning period. Pinzgauers had the highest BRD frequency within the feedlot (24.6%). The heritability estimates of BRD during the preweaning and postweaning periods did not differ significantly from 0 (.10 +/- .02 and .06 +/- .07, respectively). Although it is likely that response to selection for resistance to BRD would be slight using the animal's history of BRD as the selection criterion, including information on relatives or additional immune traits may improve the accuracy of an estimated breeding value for BRD resistance.  相似文献   

Dynamics of the Cumulation of Macroelements – Phosphorus, Potassium, Calcium and Magnesium During Development to Maturity of Soybean Seed     

V. Slip&#;evi&#;    I. Vedrina-Dragojevi&#;  L. Balint  J. Momirovi&#;-&#;uljat 《Journal of Agronomy and Crop Science》1992,168(2):73-84

Dynamics of the cumulation of phosphorus, potassium, calcium and magnesium in developing seed of four soybean genotypes under the defined climatic conditions in the course of three years was observed. Samples were being taken at weekly intervals from the point at which the grain reached a size suitable to be analyzed up to the full maturity of the seed. By analyzing the results it was found out that the intense cumulation of the minerals tested took place during the stages III and IV of the seed development, that is, in the course of intense cumulation of dry matter and the most expressive metabolic activity in the seed. Investigation of the impact of climatic conditions provides evidence that cumulation of the tested macroelements has been favourably influenced by increased precipitation along with moderate air temperatures within the seed developing period. The highest content of the minerals found with the cultivar Maksimirka independently of the year investigated reveals that mineral cumulation in soybean seed is affected by the cultivar and genetic particularity.  相似文献   

Nephrolithiasis resulting in intermittent ureteral obstruction in a cow     

T J Divers  V B Reef  K A Roby 《The Cornell veterinarian》1989,79(2):143-149

Bilateral nephrolithiasis with intermittent ureterolithiasis was diagnosed in a 7-year old Holstein cow. Two episodes of ureterolithiasis resulted in severe azotemia which resolved after spontaneous movement of the stone. A third episode of obstruction one year after the initial episode resulted in rupture of one kidney, necessitating euthanasia. The histopathological examination of the kidney was diagnostic for chronic pyelonephritis. Corynebacterium sp. was cultured from a nephrolith. In this case it is believed that the chronic pyelonephritis predisposed to the calculi formation.  相似文献   

Effect of a new pelleting process on the level of contamination of poultry mash by Escherichia coli and Salmonella   总被引：2，自引：0，他引：2  

R H McCapes  H E Ekperigin  W J Cameron  W L Ritchie  J Slagter  V Stangeland  K V Nagaraja 《Avian diseases》1989,33(1):103-111

The efficacy of a new pelleting process in eliminating naturally occurring Escherichia coli and salmonella from poultry mash was assessed by comparing the microbial load in raw and processed mash. Instead of using steam produced in a boiler, the new process conditioned mash in an Original Vertical Conditioner with steam and other hot gases generated by direct combustion in a Vaporator. E. coli was isolated from 72-100% of samples of raw mash in all trials, and the mean number of colony-forming units of E. coli/g of samples was 6.8 +/- 4.0 X 10(4). Salmonellae (S. senftenberg, S. bredeney, and S. mbandaka) were isolated from 5-10% of samples of raw mash utilized in three of the seven trials. Within limitations of the sampling and analytical tests utilized, the new pelleting process eliminated salmonella from all mash in which the organism was known to be present but eliminated E. coli in only three trials. The process appeared to be 100% effective against both organisms when mash entering the pellet mill from the conditioner had a temperature of 85.7 C and a moisture content of 14.5% and had been retained and treated with steam and hot gases for 4.1 minutes.  相似文献   

Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies     

Y Zhou  V Moennig  C O Coulibaly  J Dahle  B Liess 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(1):76-80

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.  相似文献   

Urinary protein level determination as a rapid method for the detection of contamination of slaughtered swine     

K Fehlhaber  G Krüger  V Schubert  H Rolletschek 《Archiv fuer experimentelle veterinaermedizin》1989,43(6):855-862

Reference is made to work undertaken by Stehle (1983) on rapid semiquantitative determination of urinary protein in pigs for slaughter to identify premortal strain and stress, with additional studies being conducted into the suitability of the method. The investigations performed for this study covered urinary protein samples from 59 living and 762 slaughtered pigs. The Biophan E paper strip test was applied to groups of animals, and, when compared to high-accuracy laboratory determination of urinary protein, it provided sufficiently accurate information on the presence of stress-related proteinuria. Its accuracy, however, proved to be insufficient, when individual animals were examined. The number of clearly stressed slaughter pigs was unexpectedly high, although the same animals had been rated clinically inconspicuous prior to slaughter. Brief rest period resulted in significant rise of stress. The Biophan E paper strip test was found to be suitable for instantaneous determination of stress and strain on pigs for slaughter.  相似文献