The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes     

Petr Samec  Vít Našinec 《Euphytica》1996,91(2):229-234

Summary The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution.  相似文献   

Evapotranspiration predictions of CERES-Sorghum model in Southern Italy   总被引：1，自引：0，他引：1  

A. Castrignan  V. Di Bari  M. Stelluti 《European Journal of Agronomy》1997,6(3-4):265-274

The purpose of this study was to modify and calibrate the CERES-Sorghum water balance model for the dry, high radiation and windy conditions in an area in Southern Italy.

The equation for estimating potential evapotranspiration (E₀) was substituted by another one, calibrated in the study site and expressed as a function of equilibrium evaporation and maximum vapour pressure deficit (defined as the difference between the saturation vapour pressure at maximum and at minimum temperatures).

To calibrate the E₀ equation included in CERES-Sorghum, two drainage lysimeters, located at the Istituto Sperimentale Agronomico experimental farm, Foggia (Italy), were used to measure weekly evapotranspiration of well-watered, irrigated fescue grass, from 1976 to 1986.

A further drainage lysimeter, located in the same farm and cropped with well-watered grain sorghum (cv. NK 121) was used to calibrate the genetic coefficients input to the modified CERES-Sorghum model during the cropping seasons 1979 and 1980.

Simulated phenological dates (anthesis and maturity), grain yield, LAI, biomass and crop evapotranspiration were then compared with the measured ones in a fourth drainage lysimeter cropped with sorghum.

The modified model simulated grain yield accurately, but simulated daily evapotranspiration did not always match well the observed value, especially early in the crop cycle. Improvements are needed to the model in its simulation of soil evaporation and in the crop response function to temperature.  相似文献   

Effects of Deficit Drip Irrigation Ratios on Cotton (Gossypium hirsutum L.) Yield and Fibre Quality     

H. Basal  N. Dagdelen  A. Unay  & E. Yilmaz 《Journal of Agronomy and Crop Science》2009,195(1):19-29

Increasing irrigation costs and declining water availability compel producers to adapt irrigation strategies for maximum crop yield and water use efficiency. A field trial was conducted to observe the effects of various drip irrigation ratios (IR-0, IR-25, IR-50, IR-75 and IR-100) on water use efficiency (WUE), the irrigation water use efficiency (IWUE), lint yield, yield components and fibre quality at two upland cotton varieties during 2004 and 2005. WUE was found to increase from 0.62 to 0.71 kg m⁻³ as the irrigation water applied was reduced from 100 % to 75 % of soil water depletion. Deficit irrigation of cotton with drip irrigation at 75 % treatment level (IR-75) did not decrease seed cotton yield and yield components during 2 years, with the exception of the number of bolls in 2005. Among fibre quality parameters, no significant differences in fibre length, fineness, uniformity index and elongation were detected between the 100 % and 75 % irrigation levels in 2005. The results revealed that irrigation of cotton with a drip irrigation method at 75 % level had significant benefits in terms of saved irrigation water without reducing yield, and high WUE indicated a definitive advantage of employing deficit irrigation under limited water supply conditions.  相似文献   

The inheritance of chemical phenotype in <Emphasis Type="Italic">Cannabis sativa</Emphasis> L. (III): variation in cannabichromene proportion     

E. P. M. de Meijer  K. M. Hammond  M. Micheler 《Euphytica》2009,165(2):293-311

The mechanism that controls the proportion of cannabichromene (CBC), a potential pharmaceutical, in the cannabinoid fraction of Cannabis sativa L. is explored. As with tetrahydrocannabinol (THC) and cannabidiol (CBD), CBC is an enzymatic conversion product of the precursor cannabigerol (CBG). CBC is reported to dominate the cannabinoid fraction of juveniles and to decline with maturation. This ontogeny was confirmed in inbred lines with different mature chemotypes. A consistent CBC presence was found in early leaves from a diverse clone collection, suggesting that CBC synthase is encoded by a fixed locus. Morphological variants possessing a ‘prolonged juvenile chemotype’ (PJC), a substantial proportion of CBC persisting up to maturity, are presented. PJC is associated with a reduced presence of floral bracts, bracteoles, and capitate-stalked trichomes. Genetic factors causing these features were independent of the allelic chemotype locus B that was previously postulated and regulates THC and CBD synthesis and CBG accumulation. In contrast to previously described Cannabis chemotypes, the cannabinoid composition of PJCs showed plasticity in that reduced light levels increased the CBC proportion. The ability of PJC plants to enable the production of pharmaceutical raw material with high CBC purity is demonstrated.  相似文献   

The Optimization for Crop Planning and Some Advances for Water-Saving Crop Planning in the Semiarid Loess Plateau of China     

Y. B. Wang  P. T. Wu  X. N. Zhao  J. L. Li  L. Lv  & H. B. Shao 《Journal of Agronomy and Crop Science》2010,196(1):55-65

The establishment of water-saving crop planning is an inevitable choice of the water-saving agriculture for the water-deficiency region in the arid and semiarid Loess Plateau of China and the world. The water-saving crop planning refers to the planting structure that centres the adjustment of the crop's adaptation to water, the optimization of temporal and spatial layout for crops, the local natural resources, marketing resources, human resources and financial input to enable region or basin with limited water resources to achieve the maximum economic, social and ecological benefits of planting industry under certain technology and economy. After the analysis on the research progress of optimization theory, optimization goals, optimization methods of water-saving cultivation structure and macro-control measures, it is pointed out that the main deficiencies of the current research of water-saving cultivation pattern optimization are lacking of a strong theoretical basis, and the immaturity of optimization technologies. The future crucial research direction will focus on five aspects such as the special optimization theory system, the division methods by studying the watershed unit and using 3S technology, optimization model based on multi-objective evolutionary algorithm, evaluation of rationality and macro-control measures on the basis of the public participation.  相似文献   

Production of mutants with high cold tolerance in spring canola (<Emphasis Type="Italic">Brassica napus</Emphasis>)     

Scott L. McClinchey  Laima S. Kott 《Euphytica》2008,162(1):51-67

A major factor affecting spring canola (Brassica napus) production in Canada is killing frosts during seedling development in the spring and seed maturation in the fall. The objective of this study was to explore the possibility of producing spring canola lines with mutations that have altered biochemical pathways that increase cold tolerance. The approach was to generate UV point mutations in cultured microspores followed by chemical in vitro selection of individual mutant microspores or embryos resulting in measurable alterations to various biochemical pathways with elevated levels of key defense signaling molecules such as, salicylic acid (SA), p-Fluoro-d,l-Phenyl Alanine (FPA), and jasmonic acid (JA). In addition, since proline (Pro) is known to protect plant tissues in the cold-induced osmotic stress pathway, mutants that overproduce Pro were selected in vitro by using three Pro analogues: hydroxyproline (HP), azetidine-2-carboxylate (A2C); and, 3,4-dehydro-d,l-proline (DP). Of the 329 in vitro selected mutant embryos produced, 74 were identified with significant cold tolerance compared to their donor parents through indoor freezer tests at −6°C, and 19 had better winter field survival than winter canola checks. All chemically selected mutant doubled haploids with increased cold tolerance compared well with parent lines for all seed quality and agronomic parameters. Development of increased frost tolerant cultivars should allow for spring canola to be produced in western Canada without compromising seed quality.  相似文献   

The genetic relationship between popping expansion volume and two yield components in popcorn using unconditional and conditional QTL analysis   总被引：2，自引：0，他引：2  

Y. L. Li  Y. B. Dong  D. Q. Cui  Y. Z. Wang  Y. Y. Liu  M. G. Wei  X. H. Li 《Euphytica》2008,162(3):345-351

Popping expansion volume (PEV) is the most important quality trait in popcorn, while its germplasm is inferior to normal dent/flint corn in yield. In this study, 259 F_2:3 families, developed from the cross between a dent corn inbred Dan232 and a popcorn inbred N04, were evaluated for their PEV, grain weight per plant (GWP) and 100-grain weight (100 GW) in two environments. The genetic relationship between PEV and GWP, and 100 GW on individual gene loci were evaluated using unconditional and conditional QTL mapping methods. In total, five, one and three unconditional QTL were identified for PEV, GWP, and 100 GW, respectively. The positive alleles of all QTL for PEV were from N04, while positive alleles of all QTL for GWP and 100 GW were from Dan232. In conditional mapping, one and two QTL failed to be detected, and all four additional QTL were detected. Nevertheless, three QTL were identified, which controlled PEV independently from GWP/100 GW. They seemed to be potential candidates in popcorn breeding to increase PEV without decreasing GWP/100 GW. The results suggested that for significantly correlated traits, the conditional QTL mapping method could be used to dissect the genetic interrelationship between traits at the level of individual QTL, as well as reveal additional QTL that were undetectable by unconditional mapping.  相似文献   

宇宙射线中子法测量区域土壤水分准确性季节变化研究     

武强  黄娜  罗孳孳  唐余学  朱玉涵  徐倩倩 《农业机械学报》2022,53(1):321-330

为证实典型气象条件下宇宙射线中子法测量区域土壤水分准确性的差异和应用普适性,以季节为时间划分尺度,并选取典型气象条件时段,对比研究山地地形下垫面宇宙射线中子法(CRNS)与频域反射法(FDR)土壤水分连续观测效果差异.结果 表明:山地地形下垫面,CRNS水平测量足迹的季节变化较为稳定,垂直足迹在耕作层范围内有一定波动....  相似文献   

不同工况下Y型网式过滤器流场数值模拟分析   总被引：1，自引：0，他引：1  

喻黎明  刘凯硕  韩栋  仵峰  李娜  崔宁博 《农业机械学报》2022,53(2):346-354

为探究网式过滤器的水力性能,充分了解网式过滤器内部最初流场、滤芯网面流量分布情况,应用计算流体动力学方法对网式过滤器3种入口流速(0.5、1.5、2.5 m/s)以及3种滤网目数(60、80、100目)对过滤器流场进行数值模拟。通过试验对模拟结果的可靠性进行验证,结果表明：过滤器的水头损失集中在出口侧滤芯上,该部分水头损失占总损失的87%;水流在腔体内可分为出口侧加速区、出口侧减速区、堵头回流区和漩涡区4部分;滤网面流量分布严重不均,高流量区域主要分布在出口侧,入口流速由0.5 m/s增至2.5 m/s过程中,网面最大与最小流量均相差3.3倍,滤网目数为60、80、100目时,网面最大与最小流量相差3.3、3.1、2.3倍,且滤网目数增至100目时,最大与最小流量位置向两侧偏移;堵头处死水区压力大、流速低,泥沙易于沉淀,建议扩大堵头容积以承接更多的泥沙;可以考虑增大腔体体积、改变腔体角度、在入口处设置导流片,从而改善流场分布;建议在滤网上增加环状片体,改善网面流量分布,从而提高过滤器的使用寿命以及过滤效率。  相似文献   

Origin and genetic diversity of Tunisian grapes as revealed by microsatellite markers     

N. Zoghlami  L. Riahi  V. Laucou  T. Lacombe  A. Mliki  A. Ghorbel  P. This 《Scientia Horticulturae》2009,120(4):479-486

Ten SSR loci, previously developed for grapevine, were analyzed to evaluate the genetic variability, cultivar relatedness, and parentage in a collection of 61 autochthonous Vitis vinifera cultivars from Tunisia.The number of alleles per locus ranged from 6 to 11, while the number of genotype patterns varied between 10 and 21. The expected heterozygosity varied between 0.621 and 0.855 and the observed heterozygosity was higher than 0.9 at 4 loci (VVMD28, VVMD5, VVIP31 and VVS2) indicating that the SSRs were highly informative.Cluster analysis using unweighted pair group method with arithmetic averaging (UPGMA) suggested 14 groups among studied cultivars and 53 grapevine denominations out of 61 were unequivocally distinguished, with all accessions showing at least one-specific combination of alleles.On the other hand, in order to overcome the existing confusion in Tunisian grapevine nomenclature, of the analyzed homonymous pairs of cultivars, only ‘Balta 2’ and ‘Balta 3’ have shown identical allelic profiles, consistent with their being the same genotype. Hence, nomenclature distinction is meaningless and only one denomination should be retained.Due to the high overall power of exclusion (Q) (greater than 99.99%) and to the absence of null alleles, the set of microsatellite loci used is appropriate to determine parentage in Tunisian grapevines beyond any reasonable doubt. The analysis of fingerprints indicated that the Tunisian grape vines have evolved through out crossing between five possible parents: Balta 1, Beldi Baddar, Beldi Rafraf, Beldi Local Rafraf and Khedhiri 3.  相似文献