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DebRoy C Roberts E Fratamico PM 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2011,12(2):169-185
Lipopolysaccharide on the surface of Escherichia coli constitutes the O antigens which are important virulence factors that are targets of both the innate and adaptive immune systems and play a major role in host-pathogen interactions. O antigens are responsible for antigenic specificity of the strain and determine the O serogroup. The designation of O serogroups is important for classifying E. coli strains, for epidemiological studies, in tracing the source of outbreaks of gastrointestinal or other illness, and for linking the source to the infection. For conventional serogroup identification, serotyping by agglutination reactions against antisera developed for each of the O serogroups has been used. In the last decade, many O-antigen gene clusters that encode for the enzymes responsible for the synthesis of the variable oligosaccharide region on the surface of the bacteria have been sequenced and characterized. Unique gene sequences within the O-antigen gene clusters have been targeted for identification and detection of many O groups using the polymerase chain reaction and microarrays. This review summarizes current knowledge on the DNA sequences of the O-antigen gene clusters, genetic-based methods for O-group determination and detection of pathogenic E. coli based on O-antigen and virulence gene detection, and provides perspectives on future developments in the field. 相似文献
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Stabilized municipal sewage sludge addition to improve properties of an acid mine soil for plant growth 总被引：1，自引：0，他引：1
M. D. Mingorance S. Rossini Oliva Benito Valdés F. J. Pina Gata Eduardo O. Leidi I. Guzmán Aránzazu Peña 《Journal of Soils and Sediments》2014,14(4):703-712
Purpose
Degraded soils, such as those encountered in areas of mine activities, need to be ameliorated by liming to correct soil acidity and by addition of organic inputs to improve soil properties and fertility.Materials and methods
Non-amended mine soil and soil amended with stabilized sewage sludge were incubated for 45 days. Soil physicochemical and biological indicators were periodically measured along incubation and other enzyme activities at the end of incubation. In improved soils, a study of plant development in 250-g pots was carried out with three vegetal species: tomato, rye grass and ahipa. Germination and mortality rates, biomass production and photosynthetic pigments were measured.Results and discussion
Soil incubation with sewage sludge slightly increased soil pH and led to an enhancement of soil electrical conductivity, organic carbon and dehydrogenase activity, especially for the higher doses (5 and 10%). However soil respiration was more promoted with the 2% dose, pointing to a possible toxic effect of the sludge. At the end of incubation, physicochemical and biological properties were in general enhanced. Biomass production was improved in tomato and rye grass by sewage sludge addition (more at the 2% dose), whilst ahipa growth was not affected by sewage sludge treatments. Tomato mortality reached 73% with high sludge doses (10%).Conclusions
According to this set of parameters, amendment with sewage sludge of a limed acid mine soil would be considered as a good strategy for soil amelioration in view of plant establishment and development. 相似文献14.
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Abstract – In this work we aim to study: (i) the diel and seasonal changes in nutritional condition, herein RNA/DNA ratios, of twaite shad ( Alosa fallax fallax ) larvae and (ii) the influence of environmental and biological covariates on those changes. Samples were collected in the River Mira (Portugal) from February 1998 to July 2000. The RNA/DNA ratios were significantly lower than average during dawn and dusk hours. This pattern can be attributed to: the absence of better-fit specimens escaping the net; a nightly increase in the RNA content in response to circadian rhythm; and/or nucleic acids of prey items remaining in the gut of larvae during daylight. Thus, the time of day is important when examining RNA/DNA ratios of twaite shad. Generalised additive model of seasonal data showed that water temperature, freshwater pulses and/or turbidity largely contribute to create adequate environments. These might enhance the nutritional condition of twaite shad larvae during the period that they remain in the upper reaches of River Mira. 相似文献
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Maribel Casas Raquel Cortés Sonia Pina Bibiana Peralta Alberto Allepuz Martí Cortey Jordi Casal Marga Martín 《Veterinary microbiology》2011
Hepatitis E is a zoonotic disease and is highly prevalent in European swine livestock. There is a need to compare the infection dynamics of hepatitis E virus (HEV) between herds with the same production system and determine the percentage of animals that could arrive infected at slaughter age. Therefore, a longitudinal study was performed in six Spanish farrow-to-finish affected farms. Twenty piglets per farm were monitored from nursery to slaughter. RT-PCR and serology techniques were applied to analyze longitudinally collected sera and/or faecal samples. Liver and bile samples were also taken at the abattoir. Anti-HEV IgM were firstly detected at 7 weeks of age in 5 farms whereas at 13 weeks of age in 1 farm (farm 2). At slaughter age 50–100% of pigs had seroconverted to anti-HEV IgG in the former 5 farms whereas in the other herd only 5% of pigs were IgG seropositive (farm 2). Six out of 96 livers and 5 out of 80 biles analyzed were HEV positive at the abattoir (total percentage of infected animals: 11.5%). All these positive animals had already seroconverted except 2 pigs of farm 2. Hence, pigs can be seronegative at slaughter age being infected during the latest fattening period. Manipulation of HEV-infected livers or other organs from pigs could be considered a possible route of transmission in Spanish abattoirs. This study represents the first longitudinal survey on swine HEV infection dynamics conducted in different herds. 相似文献
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Barbosa AM Valadares RF Valadares Filho SC Pina DS Detmann E Leão MI 《Journal of animal science》2011,89(2):510-519
Two experiments were conducted to assess the endogenous fraction of purine derivative (PD) excretion, urinary recovery, and intestinal digestibility of purines in Nellore heifers. For both experiments, 8 Nellore heifers fitted with ruminal and abomasal cannulas were allocated to two 4 × 4 Latin squares. The diets were based on corn silage and concentrate (60 and 40% DM basis, respectively); feces and urine samples were obtained by total collection, and abomasal DM flow was estimated using indigestible NDF as an internal marker. In Exp. I, 4 of the 8 heifers (BW 258 ± 20 kg) were also fitted with ileal cannula. The planned treatments were 4 different DMI: 1.2, 1.6, 2.0, and 2.4% of BW (DM basis). The endogenous losses and purine recovery as urinary PD were estimated using linear regression between daily urinary PD excretion (Y) and daily abomasal flow of purine bases (X), expressed in millimoles per kilogram of BW(0.75). In Exp. II, the same 8 Nellore heifers (BW of 296 ± 15 kg) were fed at 1.37% BW (DM basis). The treatments were the infusion of purines (RNA from torula yeast, type VI, Sigma) into the abomasum in increasing amounts (0, 33, 66, and 100 mmol/d). All statistical analyses were performed using the PROC MIXED procedure in SAS. In Exp. I, the DMI range was 1.16 to 1.84% of BW and did not affect (P > 0.05) the apparent RNA digestibility in the small intestine, which had a mean of 75.6%, and a true digestibility of 93.0%. The mean ratio of the N-RNA to the total-N in the ruminal bacteria was 0.137. The daily urinary PD excretion (Y, mmol/kg of BW(0.75)) was a function of RNA flow in the abomasum (X, mmol/kg of BW(0.75)): Y = 0.860X + 0.460, where 0.860 and 0.460 were the PD recovery of purines and the endogenous fraction (in mmol/kg of BW(0.75)), respectively. In Exp. II, the daily urinary PD excretion was a function of RNA flow in the abomasum: Y = 0.741X + 0.301, where 0.741 and 0.301 were the recovery of PD in urine of infused purines and the endogenous losses (in mmol/kg of BW(0.75)), respectively. In conclusion, our data suggest that in Nellore heifers the respective values of endogenous PD excretion (mmol/kg of BW(0.75)), urinary recovery of the purines absorbed in the abomasum, and true digestibility of RNA in the small intestine were 0.30, 0.80, and 0.93. 相似文献
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The present study describes the anatomy and surface topography of the metacercaria of Microphallusprimas (J?gerski?ld, 1909) infecting the shore crab Carcinus maenas (L.) in Aveiro estuary, northern Portugal. The metacercaria species identification resulted from the combined use of morphological and molecular data, particularly the 28S rDNA gene. The metacercariae encysted preferentially in the host's hepatopancreas and also in the gonads. Isolated cysts were present in two distinct forms, spherical and oval, and were shown to be the identical species by the internal transcribed spacer 1 (ITS1) sequence. Chemically excysted metacercariae were studied by light (LM) and scanning electron microscopy (SEM). Their specific characteristics observed include the particular aspect of the vesiculo-prostatic pouch surrounded by a very thin membrane, the presence of a prominent muscular papilla, and an obvious metraterm. The dorsal and ventral tegumental surfaces of the metacercaria were densely packed with similar squamous spines, which decreased in number and size towards the hindbody. The edges of the posterior and ventral face of the body were coated with numerous microvilli, whose function remains unknown. In order to identify the species of metacercariae, we compared a 28S partial rDNA sequence of the two forms of cysts with the same 28S partial region of M. primas available in GenBank. With this comparison, we determined that the sequences had a 100% similarity and therefore belonged to the same species, i.e., M. primas. 相似文献
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Abdelkafi S Barouh N Fouquet B Fendri I Pina M Scheirlinckx F Villeneuve P Carrière F 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):34-40
Triacylglycerol (TAG) lipases have been thoroughly characterized in mammals and microorganisms, whereas very little is known
about plant TAG lipases. The lipolytic activity occurring in all the laticies is known to be associated with sedimentable
particles, and all attempts to solubilize the lipolytic activity of Carica papaya latex have been unsuccessful so far. However, some of the biochemical properties of the lipase from Carica papaya latex (CPL) were determined from the insoluble fraction of the latex. The activity was optimum at a temperature of 37°C and
a pH of 9.0, and the specific activities of CPL were found to be 2,000 ± 185 and 256 ± 8 U/g when tributyrin and olive oil
were used as substrates, respectively. CPL was found to be active in the absence of any detergent, whereas many lipases require
detergent to prevent the occurrence of interfacial denaturation. CPL was inactive in the presence of micellar concentrations
of Triton X-100, sodium dodecyl sulfate (SDS) and tetradecyl trimethylammonium bromide (TTAB), and still showed high levels
of activity in the presence of sodium taurodeoxycholate (NaTDC) and the zwitterionic Chaps detergent. The effects of various
proteases on the lipolytic activity of CPL were studied, and CPL was found to be resistant to treatment with various enzymes,
except in the presence of trypsin. All these properties suggest that CPL may be a good candidate for various biotechnological
applications. 相似文献