Ellagic Acid and Polyhydroxylated Urolithins Are Potent Catalytic Inhibitors of Human Topoisomerase II: An in Vitro Study     

V Furlanetto  G Zagotto  R Pasquale  S Moro  B Gatto 《Journal of agricultural and food chemistry》2012,60(36):9162-9170

Ellagic acid (EA), a natural polyphenol abundant in fruits and common in our diet, is under intense investigation for its chemopreventive activity resulting from multiple effects. EA inhibits topoisomerase II, but the effects on the human enzyme of urolithins, its monolactone metabolites, are not known. Therefore, the action of several synthetic urolithins toward topoisomerases II was evaluated, showing that polyhydroxylated urolithins, EA, and EA-related compounds are potent inhibitors of the α and β isoforms of human topoisomerase II at submicromolar concentrations. Competition tests demonstrate a dose-dependent relationship between ATP and the inhibition of the enzyme. Docking experiments show that the active compounds bind the ATP pocket of the human enzyme, thus supporting the hypothesis that EA and polyhydroxylated urolithins act as ATP-competitive inhibitors of human topoisomerase II.  相似文献   

The role of border areas for dendrochronological investigations on catastrophic snow avalanches: A case study from the Italian Alps     

Valentina Garavaglia  Manuela Pelfini 《CATENA》2011

Snow avalanches are common in mountain environments, sometimes affecting inhabited areas and infrastructures (e.g. roads, bridges, and ski slopes). Their study is widespread and involves the use of a variety of different techniques, including dendrochronological methods. The aim of such investigations is principally to date past events, but also to detect variation in avalanche frequency as well as their spatial distribution. Trees affected by snow avalanches generally have scars, tilted trunks and broken branches, which allow past events to be dated to within a year. In this work tree rings were used to investigate a disruptive snow avalanche which occurred in 2001 in Val Mala in the Italian Alps. The event almost completely removed forest along the flow path, while the snow powder component of the avalanche also impacted on the adjacent forest. Comparison of tree reaction in surviving plants along the flow path and vegetation on the border showed i) the production of reaction wood even in apparently undisturbed trees and ii) the usefulness of border plants for dating past events. Different dendroecological indicators were investigated (i.e. reaction wood, scars, traumatic resin ducts, and variations in stem eccentricity) and reaction wood is evidently concentrated from 2001 not only in damaged trees but also in adjacent plants. The potential to investigate past snow avalanches by collecting samples from trees growing in border areas is presented: in the specific case of Val Mala, vegetation bordering the flow paths reacted to produce reaction wood in 2001, in a similar manner to plants along the flow track, demonstrating their usefulness in such investigations. Their response to the 2001 event confirms the possibility of applying dendrochronological techniques to trees adjacent to the flow path, even if they appear morphologically undisturbed, and proposes to use only border areas in cases of an absence of sufficient trees along the avalanche track.  相似文献   

Cytokine expression, glucocorticoid and growth hormone changes after porcine reproductive and respiratory syndrome virus (PRRSV-1) infection in vaccinated and unvaccinated naturally exposed pigs     

Paolo Borghetti  Roberta SaleriLuca Ferrari  Marina MorgantiElena De Angelis  Valentina FranceschiEzio Bottarelli  Paolo Martelli 《Comparative immunology, microbiology and infectious diseases》2011,34(2):143-155

The objective of this paper was to study the changes of some cytokines and neuroendocrine hormones in vaccinated and unvaccinated pigs that were naturally infected by a PRRSV-1 (porcine reproductive and respiratory syndrome virus) heterologous field strain. We analyzed gene expression of pro-inflammatory (TNF-α, IL-1β, MCP-1, IL-6), pro-immune (IFN-γ) and anti-inflammatory cytokines (IL-10) in PBMC, as well as hormonal (GH and cortisol) levels in blood samples of pigs obtained in a field trial previously reported [Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: clinical protection and cell-mediated immunity. Vaccine 2009;27:3788-99]. All vaccinated pigs showed an increase in pro-inflammatory and pro-immune cytokine gene expression with respect to controls and a prompt increase in GH that could be consistently associated with pro-inflammatory cytokines in sustaining innate immunity; moreover, the higher levels of cortisol indicates the activation of the hypothalamus-pituitary-adrenal (HPA) axis response. In contrast, unvaccinated pigs showed down-regulation of the cortisol and GH responses, and the pro-inflammatory and pro-immune cytokines remained at a basal or low level, with an increase of TNF-α and IL-6 in association with a higher level of IL-10 in the late phase of natural infection. The associated trends of pro-inflammatory and anti-inflammatory cytokines together with the cortisol level demonstrate that a previous vaccination promotes an early immune responsiveness in pigs and a more efficient control of inflammation in the late phase of infection with a heterologous PRRSV isolate; both events could sustain clinical protection.  相似文献   

A prospective multicenter observational study of Viperidae polyvalent immune F(ab’)2 antivenom administration for the treatment of viper envenomation in dogs     

Michele Vanni  Luigi Intorre  Michele Corazza  Valentina Meucci  Sandro Parti 《Journal of Veterinary Emergency and Critical Care》2020,30(1):34-40

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First report of equine parvovirus-hepatitis-associated Theiler’s disease in Europe     

Modest Vengust  Mason C. Jager  Valentina Zalig  Vasilij Cociancich  Melissa Laverack  Randall W. Renshaw  Edward Dubovi  Joy E. Tomlinson  Gerlinde R. Van de Walle  Thomas J. Divers 《Equine veterinary journal》2020,52(6):841-847

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Growth Responses,Metal Accumulation and Phytoremoval Capability in Amaranthus Plants Exposed to Nickel Under Hydroponics     

Iori  Valentina  Pietrini  Fabrizio  Cheremisina  Alexandra  Shevyakova  Nina I.  Radyukina  Nataliya  Kuznetsov  Vladimir V.  Zacchini  Massimo 《Water, air, and soil pollution》2013,224(2):1-10

Surfactant-enhanced solubilization and subsequent biodegradation of phenanthrene and pyrene from aqueous solutions by Arthrobacter strain Sphe3 was investigated. The results show that growth of Arthrobacter strain Sphe3 was increased upon increase in concentration of Tween 20 and Tween 80. Inhibition of bacterial growth was observed with increasing Triton X-100 concentrations, whereas sodium dodecyl sulfate (SDS) totally inhibited this bacterial growth. Phenanthrene and pyrene solubilization was enhanced in the presence of surfactants and found to be linearly proportional to their concentrations, above the critical micelle concentration (CMC). In addition, Tween 20 and Tween 80 enhanced the biodegradation of phenanthrene and pyrene. The high correlation coefficient (R ²) values obtained at all the concentrations studied, suggest that biodegradation kinetics of both phenanthrene and pyrene in the presence of Tween 20 and Tween 80 follow first-order kinetic equation model. Experimental results suggest that Tween 20 and Tween 80 may have great potential for applications in bioremediation of these polycyclic aromatic hydrocarbon (PAH) compounds using Arthrobacter strain Sphe3.  相似文献   

Comparison of smartphone‐based and standard base‐apex electrocardiography in healthy dairy cows     

Francesca Bonelli  Tommaso Vezzosi  Mireille Meylan  Irene Nocera  Vincenzo Ferrulli  Carlotta Buralli  Valentina Meucci  Rosalba Tognetti 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):981-986

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Retrospective Biomolecular Investigation of Coxiella burnetii and Leptospira spp. DNA in Cases of Abortion,Stillbirth and Neonatal Mortality in Dogs and Cats     

Valentina Stefanetti  Agnese Compagnone  Chiara Sordini  Fabrizio Passamonti  Elisa Rampacci  Livia Moscati  Maria Luisa Marenzoni 《Topics in companion animal medicine》2018,33(4):122-125

Abortion and neonatal mortality are events that can occur in breeding bitches and queens. It has been reported that up to 55% and 33% of these cases remain without a known cause, respectively, in canine and feline pregnancies. Unusual abortigenic and potentially zoonotic agents, including Coxiella burnetii and Leptospira spp., may be involved in these cases. C. burnetii is able to cause reproductive disorders in cattle, sheep and goats, and cases of abortion have been observed in dogs and cats. Moreover, several outbreaks of C. burnetii infection in humans have been caused by delivering bitches and queens, and some of these animals experienced abortion. Leptospira interrogans sensu lato is able to cause abortion or stillbirth in several animal species and its abortigenic role has occasionally been described in bitches and queens. The aim of this study was to search for C. burnetii and Leptospira spp. DNA in a retrospective series of 103 cases of canine and feline abortion, stillbirth, and neonatal mortality submitted for the identification of possible infectious agents. One hundred and fifty-one specimens were tested using PCR assays and found negative for C. burnetii and Leptospira DNA. However, in 49 samples (47.6%) other infectious causes of abortion, stillbirth, and neonatal mortality were identified. These results showed that C. burnetii and Leptospira spp. are probably not common abortigenic agents or causes of neonatal deaths in dogs. However, given the potential abortigentic and zoonotic role of these agents, surveillance of canine and feline abortion, stillbirth, and neonatal mortality could be advisable for a systematic investigation of these events.  相似文献   

Towards a comprehensive education and training program in agroforestry     

Valentina Dagien?  Gintautas Dzemyda  Mifodijus Sapagovas 《Agroforestry Systems》1990,12(1):121-131

Workshop Summary and Synthesis

Towards a comprehensive education and training program in agroforestry  相似文献   

Pollen morphology as fertility predictor in hybrid tea roses     

Luca Pipino  Marie-Christine Van Labeke  Andrea Mansuino  Valentina Scariot  Annalisa Giovannini  Leen Leus 《Euphytica》2011,178(2):203-214

Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown. In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised. Pollen was either small (mean diameter <30 μm), shrunken, and irregular (abnormal), or large (mean diameter >30 μm), elliptical and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes as male parents.  相似文献