Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs     

Erik Asimus  Sophie Palierne  Margaux Blondel  Valentine Pollet  Aude Ferran  Alain Bousquet‐Melou  Jean‐Franois Rousselot  Andr Autefage 《Veterinary surgery : VS》2019,48(8):1466-1472

  相似文献   

人工感染猪附红细胞体病的病理组织学研究   总被引：1，自引：1，他引：0  

尤立娜  巴彩凤  冯会权  李慧平  王凯  林小丰  廉长红  石蕾 《中国畜牧兽医》2008,35(6)

为观察昆明小鼠人工感染猪附红细胞体后的病理组织学变化。用常规病理学方法,对6只腹腔注射感染猪附红细胞体的小鼠进行病理解剖学和病理组织学研究,光镜下观察并描述小鼠红细胞感染附红细胞体后的形态变化。剖检可见主要器官和皮下瘀血,胸腹部皮下脂肪黄染,病理组织学变化的主要特征是心、肝、脾、肺、肾等主要脏器实质细胞变性、坏死,毛细血管扩张充血、出血,脾窦内含铁血黄素沉着。结果表明,猪附红细胞体感染小鼠模型的成功建立,镜下红细胞的形态特征及主要器官剖检和病理组织学变化与文献报道的猪附红细胞致病特征基本吻合,存在的差异可能与不同地域猪附红细胞体病原株致病性有关。  相似文献   

Involvement of extracellular signal regulated kinase in the regulation of amyloid precursor protein processing in PC12 cells by TPPB     

YANG Hong-qi  CHEN Sheng-di  BA Mao-wen  LU Guo-qiang  LIANG Liang  XU Jie-yi 《园艺学报》2008,24(1):24-27

AIM: To explore the signal transduction pathways involved in the regulation of amyloid precursor protein (APP) processing by protein kinase C (PKC) activator TPPB.METHODS: PC12 cells were treated with TPPB (PKC activator) for 3 h and various signal transduction inhibitors were added to the conditioned medium to investigate their effects on α-secretase form of soluble amyloid precursor protein (sAPPα) secretion after TPPB treatment via Western blotting. Extracellular signal regulated kinase (ERK, p42/44MAPK) and phospho-p42/44MAPK were also measured after TPPB treatment.RESULTS: TPPB (1 μmol/L) significantly increased sAPPα secretion as compared with control group. The increase in sAPPα secretion by TPPB was partially blocked by ERK inhibitor U0126, c-Jun N-terminal kinase (JNK) inhibitor SP600125 and protein tyrosine kinase (PTK) inhibitor genistein, but not by p38MAPK inhibitor SB203580. TPPB (1 μmol/L) increased the expression of phospho-p42/44MAPK without altering total p42/44MAPK levels.CONCLUSION: ERK, JNK and PTK may be involved in the regulation of APP processing by TPPB.  相似文献   

Ultrastructure of the hydrophobic gastric surfactant barrier in the dog   总被引：1，自引：0，他引：1  

MT ETHELL  DR HODGSON  BA HILLS 《Australian veterinary journal》1999,77(4):240-244

OBJECTIVES: To confirm the hydrophobicity of the luminal surface of the canine stomach and to elucidate the ultrastructure of the lining imparting that property. DESIGN AND PROCEDURES: Gastric and duodenal mucosal samples from eight dogs were collected immediately after euthanasia and subjected to contact angle measurement using a goniometer. Other samples were examined by electron microscopy following a fixation procedure known to preserve phospholipids and oligolamellar structures. RESULTS: Contact angles for the canine gastric mucosal surface (85.1 +/- 5.5) were significantly greater (P < 0.0001) than for the duodenum (24.0 +/- 1.7). Electron microscopy revealed the existence of surfactant as abundant osmiophilic phospholipid material within the gastric and duodenal mucosae. CONCLUSION: We have confirmed the hydrophobic nature of the canine gastric mucosa whereas the luminal surface of the duodenum is hydrophilic. We propose that the water-repellent nature of the canine gastric lining contributes to the 'gastric mucosal barrier' and is imparted by an oligolamellar layer of surface-active phospholipid ('gastric surfactant') adsorbed to the surface. Both gastric and duodenal mucosae may also utilise phospholipids as an intercellular defense mechanism in the event that tight junctions are breached by acid. It is tempting to speculate that a deficiency of gastric phospholipids may predispose dogs to ulcers. Further, exogenous administration of phospholipids may be useful in preventing gastric ulceration.  相似文献   

CONGENITAL ATRESIA OF THE PAROTID DUCT IN A HORSE     

Valerie M.  Sadler  DVM  Erik R.  Wisner  DVM  James T.  Robertson  DVM  Valentine S.  Moses  DVM 《Veterinary radiology & ultrasound》1999,40(3):259-261

Congenital anomalies of the equine salivary glands and their ductal systems are rare. In man, parotid duct atresia is thought to be due to a congenital malformation of the first branchial arch. One horse with unilateral parotid salivary duct atresia is described. Imaging modalities available for accurate diagnosis, and treatment options, are reviewed.  相似文献   

锦江鱼类资源现状与物种多样性分析     

李忠利  冉辉  巴家文  杨天友 《淡水渔业》2017,47(2):63-70

2013年8月—2016年8月对沅江水系支流锦江的鱼类资源现状进行了调查。通过向渔民购买、现场捕捞等方式,共调查野生鱼类43种,隶属于5目11科39属,其中鲤科鱼类25种,占总种类数的58.1%。调查到新记录种5种,分别为大鳞副泥鳅(Paramisgurnus dabryanus)、吻(Rhinogobio typus)、洞庭华鲮(Sinilabeo tungting)、云南盘(Discogobio yunnanensis)和大眼鳜(Siniperca kneri)。干、支流均以底层鱼类为主,种类数分别占各自总数的55.8%和71.4%。对比历史资料,G-F多样性指数呈下降趋势。对干流渔获物种类的周年统计发现,出现频率在50%以上的鱼类有8种,其中泥鳅(Misgurnus anguillicaudatus)、鲤(Cyprinus carpio)、鲫(Carassius auratus)和鲇(Silurus asotus)的出现频率均达到97.3%。  相似文献   

黄河鲤嗜水气单胞菌的分离、毒力及用药分析   总被引：2，自引：0，他引：2  

赵贤亮  陈鹤  杨潇  巴新霞  李莉  孔祥会 《水产科学》2017,(5):642-646

自发病黄河鲤4种组织中分离致病菌,结合形态学和16SrDNA序列分析,发现分离到的细菌属气单胞菌属。回归感染试验发现,患病鱼体出现鳃盖等多处组织出血且伴随肠炎等症状,表明引起黄河鲤发病的致病菌为嗜水气单胞菌,命名为HNAh01。半致死密度测定发现,嗜水气单胞菌HNAh01的半致死密度为1.38×10~7 cfu/mL。药敏结果表明,该致病性嗜水气单胞菌是典型的多重耐药嗜水气单胞菌,但对恩诺沙星极为敏感。因此,选择恩诺沙星对分离菌株进行体外药效学和用药分析试验。药效学试验表明,恩诺沙星对嗜水气单胞菌HNAh01的最小抑菌质量浓度为1.56ng/mL,最小杀菌质量浓度为3.12ng/mL,防耐药突变质量浓度为12.5ng/mL,耐药选择窗的范围为1.56~12.5ng/mL。用药分析结果显示,黄河鲤口服剂量为10mg/kg时对嗜水气单胞菌HNAh01的相对保护率达83%。本文研究为嗜水气单胞菌的防治提供了理论基础与实践依据。  相似文献   

不同环境因子对草履虫种群生长的影响   总被引：1，自引：0，他引：1  

王兴达  曹英昆  霸婉玉  张奇  王小瑞  沈庆洲  张铁 《河北渔业》2017,(7)

为了研究不同环境因子对草履虫种群生长的影响,培养出优质的草履虫,分别在pH梯度为:6.0、6.5、7.0、7.5、8.0,光照梯度为:0lx、500lx、1 000lx、1 500lx条件下培养草履虫,结果表明,小白菜培养液培养草履虫的最适pH值为7.0,并且相比于弱酸性环境,草履虫更适宜在弱碱性环境中生长。在光照为0lx条件下,草履虫数量在第4天时达到最大值4 073ind/100mL,在光照为500~1 500lx条件下,随着光照度的增加,草履虫的种群数量也随之增加。  相似文献   

基于模型的四足机器人步态转换控制研究     

陈久朋  李春磊  伞红军  康伟  巴光玉  杨晓园 《农业机械学报》2024,55(3):431-440,451

为了解决基于模型的控制方法在四足机器人步态转换过程中稳定控制问题,本文在仿生学和机构学基础上设计了一款四足机器人样机平台,并推导了机器人单腿运动学模型。在足端可达工作空间内规划了机器人抬腿高度和迈步步长,利用理想的复合摆线轨迹,通过合理控制步态周期,提出了一种过渡段变周期控制方法,实现了步态转换前后定速度控制和变步长控制,保证了步态转换前后速度不变或可变。为了验证所提算法的正确性和稳定性,分别开展了单腿足端轨迹实验和整机步态转换实验。在完成整机运动控制的基础上,对比了基于模型的控制方式和基于中枢模式发生器的控制方式在四足机器人步态转换过程的应用。仿真和实验结果表明,在基于模型的控制算法下,四足机器人可以实现步态的平滑转换,且速度能伴随步长和周期的变化实现调节,满足了不同速度下的行走要求,为四足机器的运动控制提供了参考。  相似文献   

冬春温度变化对小麦温敏雄性不育系BNS的育性转换影响     

秦志英  孙海燕  付庆云  卫笑  巴爱丽  茹振钢  李友勇 《西北农业学报》2018,27(1):31-37

BNS是一个主体受温度控制的新型小麦雄性不育系,表现为花粉发育在低温下(8℃)不育,高温下(12℃)可育。BNS不育性稳定,但年份间常有波动,一些年份自交结实率上升显著。为了弄清楚BNS育性波动的原因,连续3a观察不同播期的BNS穗分化过程,分析连续6a调查的BNS自交结实率与对应年份的冬春温度变化,结果发现,冬季和春季温度的改变显著影响BNS的自交结实率。一般规律是,暖冬年份自交结实率低,寒冬较高,暖春年份自交结实率高,寒春较低。该现象的形成主要是BNS冬季生长发育速度所致,暖冬年份BNS冬季仍保持生长,到春季时完成温度敏感期前的发育,较早进入温度敏感期,此时的春季温度较低,因此BNS不育;然而在寒冬年份,BNS停止生长,春季需生长一定时间才进入温度敏感期,进入时气温已较高,因此诱导育性转换,自交结实率增高。BNS冬季生长发育速度在穗分化观察中得到验证。该机理解释了不同年份BNS自交结实率的波动,并根据冬春温度可预测BNS的育性转换。  相似文献