Immunohistochemical evaluation of MMP-2 and TIMP-2 in canine mammary tumours: a survival study   总被引：1，自引：0，他引：1  

Santos A  Lopes C  Frias C  Amorim I  Vicente C  Gärtner F  Matos Ad 《Veterinary journal (London, England : 1997)》2011,(3):396-402

Canine mammary tumours (CMTs) are a very heterogeneous group of neoplasms with variable prognosis. Their aggressiveness is mainly due to their ability to invade locally and to metastasize. The degradation of extracellular matrix components is an important determinant of the invasive phenotype. The aims of this study were to analyse by immunohistochemistry and double immunofluorescence the expression of metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in eight normal canine mammary glands and 118 CMTs (24 benign, 94 malignant) and to investigate relationships with metastatic disease and survival.MMP-2 and TIMP-2 expression was higher in malignant tumours than in normal canine mammary tissue and benign tumours. The main difference between benign and malignant CMTs was the pattern of expression of both molecules: benign tumours presented TIMP-2 and MMP-2 immunoreactivity in the myoepithelial cells lining the basement membrane of tubuloalveolar structures, while malignant tumours showed mainly diffuse expression in neoplastic cells. In malignant tumours, increased TIMP-2 expression was significantly associated with the development of distant metastases, lower overall survival and lower disease-free survival. MMP-2 expression was not significantly associated to any of these parameters. These results suggest that the immunohistochemical expression of TIMP-2 is a useful prognostic factor in CMTs.  相似文献   

Overweight, asthma symptoms, atopy and pulmonary function in children of 4-12 years of age: findings from the SCAALA cohort in Salvador, Bahia, Brazil     

Matos SM  Jesus SR  Saldiva SR  Prado MS  D'Innocenzo S  Assis AM  Rodrigues LC  Alcantara-Neves NM  Cruz ÁA  Simões Sde M  Barreto ML;SCAALA 《Public health nutrition》2011,14(7):1270-1278

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Neuroscience. Seeing the world in the same way     

Pessoa L 《Science (New York, N.Y.)》2004,303(5664):1617-1618

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Biochemical modulations in açaí palm (Euterpe oleracea Mart.) under vegetative stages in an agroforestry system of the Amazon     

Gizele Odete de Sousa  Ismael de Jesus Matos Vigas  Jessivaldo Rodrigues Galvo  Susana Silva Conceio  Tiago Kesajiro Moraes Yakuwa  Mauro Junior Borges Pacheco  Thiago Costa Viana 《植物养料与土壤学杂志》2021,184(1):76-87

Background : The açaí palm (Euterpe oleracea Mart.) is a species native to the Amazon region, where the largest stock of these palms is located in the state of Pará, Brazil. This species occurs predominantly spontaneously in the plateau and plains areas of the Amazonian estuary. Water deficit is the main limiting factor of plants, negatively regulating the growth, metabolism and productivity of crops. Aim : This study aimed to deepen the ecophysiological and biochemical responses of Euterpe oleracea Mart. to a water deficiency condition in agroforestry systems. Methods : The study was carried out in a defined period of lowest rainfall in the region, which runs from July to October, averaging 58.6 mm for those months, such as location, age, uniformity, health, development, height and diameter of the stipe, in order to determine the biochemical variables. Responses to biochemical components under water deficit such as starch, sucrose and carbohydrates increased for rachis and fruits. Results : The concentrations of ammonia and proteins increased in stems and fruits. For amino acids, the structures that accumulated these organic compounds were fruits and arrow. One of the most representative amino acids against water deficiency is proline, and its increase in plant tissues is due to osmoprotein function, especially for structures such as rachis, fruit and petioles + rachis. Conclusions : Therefore, Euterpe oleracea Mart. presents excellent physiological plasticity to deal with stressful conditions as well as adaptive behavior to overcome this deficiency.  相似文献   

Removal of Methylene Blue from an Aqueous Medium Using Atemoya Peel as a Low-cost Adsorbent     

Venceslau  Adneia de Fátima Abreu  Mendonça  Andressa Campos  Carvalho  Lucas Bragança  Ferreira  Guilherme Max Dias  Thomasi  Sergio Scherrer  Pinto  Luciana Matos Alves 《Water, air, and soil pollution》2021,232(11):1-17

Water, Air, & Soil Pollution - The objective of the present study was the synthesis and characterization of chitosan-alginate-based cross-linked copolymer (CACC) for the effective removal of...  相似文献   

European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil     

Bárbara Teixeira  António Marques  Cristina RamosIrineu Batista  Carmo SerranoOlívia Matos  Nuno R. NengJosé M.F. Nogueira  Jorge Alexandre SaraivaMaria Leonor Nunes 《Industrial Crops and Products》2012,36(1):81-87

There is a growing interest of industry to replace synthetic chemicals by natural products with bioactive properties from plant origin. The aim of this study was to determine the chemical composition of European pennyroyal (Mentha pulegium) essential oil and to characterize the in vitro antioxidant and antimicrobial activities of its water (hot and cold) and ethanolic extracts and of the essential oil. The essential oil revealed menthone, pulegone and neo-menthol as the main constituents, comprising 35.9, 23.2 and 9.2% of the essential oil, respectively. The hot water extract exhibited the highest antioxidant activity and phenol content. In contrast, the extracts were not very effective to inhibit the growth of the seven foodborne spoilage and pathogenic bacteria tested, but the essential oil showed antibacterial activity against all bacterial strains. In conclusion, extracts and essential oil of M. pulegium from Mediterranean origin have huge potential as an alternative to chemical additives for the food industry.  相似文献   

Plant protein and vegetable oil‐based diets modulate gilthead seabream (Sparus aurata) muscle biochemical status and proteolytic enzymes at an early postmortem stage     

E. Matos  T.S. Silva  R. Colen  M.T. Dinis  J. Dias 《Aquaculture Nutrition》2014,20(2):153-162

A 70 days feeding trial was carried out on juvenile gilthead seabream (Sparus aurata) (mean initial body weight: 362 ± 59 g) to evaluate whether the simultaneous replacement of fishmeal and fish oil by vegetable ingredients affected the muscle's proteolytic potential. Fish were fed either a fishmeal/fish oil diet, a fishmeal/vegetable oil diet, a plant protein/fish oil or a plant protein/vegetable oil diet. The use of dietary plant proteins resulted in lower sulphated glycosaminoglycans' content and lower glycogen phosphorylase activity in the muscle, while both plant proteins and vegetable oil increased muscle pH and reduced cathepsin B activity. Our results indicate that high replacement of fish meal and/or fish oil with plant ingredients has a significant impact on early postmortem metabolic processes and proteolytic potential of seabream muscle, especially when replacing both fish meal and fish oil.  相似文献   

Mucoadhesive nanoparticles: a new perspective for fish drug application          下载免费PDF全文

A C da S Costa  H M Brando  S R da Silva  A R Bentes‐Sousa  J A P Diniz  J de J Viana Pinheiro  M de F C de Melo  J O C Silva  E R Matos  R M Ribeiro‐Costa 《Journal of fish diseases》2016,39(4):503-506

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Spineless cactus associated with Tifton hay or sugarcane bagasse may replace corn silage in sheep diets     

Julimar do Sacramento Ribeiro  Luana Lira Santos  Dorgival Morais de Lima Júnior  Tobyas Maia de Albuquerque Mariz  Márcio Machado Ladeira  Paulo Sérgio de Azevedo  Carolyny Batista Lima  Maria Josilaine Matos dos Santos Silva 《Tropical animal health and production》2017,49(5):995-1000

The effect of the replacement of corn silage with spineless cactus associated with Tifton 85 hay or sugarcane bagasse was evaluated on the performance, body weight components and sheep meat quality. Twenty-one Santa Inês male sheep with initial body weight of 22.9 ± 2.9 kg were used. They were confined in individual stalls for 74 days receiving the following diets: corn silage + concentrate (CS); spineless cactus + Tifton 85 hay + concentrate (TH); spineless cactus + sugarcane bagasse + concentrate (SB). After the feedlot period, the animals were slaughtered and their body components were weighed and sampled. Data were submitted to Tukey’s test at 5% probability. The average daily gain (0.25 vs 0.14 kg), cold carcass weight (13.3 vs 13.7 kg), loin muscularity (0.6 vs 0.4 kg) and liver weight (0.7 vs. 0.5 kg) were higher (P < 0.05) for the TH diet when compared to that for the CS. The physical-chemical parameters of meat were not influenced (P > 0.05) by the treatments and presented about 20.1% of crude protein, 6.1% of crude fat and shear force of 2.0 kgf/cm². In complete diets for sheep, Tifton 85 hay associated with spineless cactus provides greater weight gain, cold carcass muscularity and organ weight when compared to those for animals fed with corn silage as exclusive roughage. Sugarcane bagasse associated with spineless cactus may replace corn silage in complete diets for sheep.  相似文献   

Matrix metalloproteinase-9 expression in mammary gland tumors in dogs and its relationship with prognostic factors and patient outcome     

Santos AA  Lopes CC  Marques RM  Amorim IF  Gärtner MF  de Matos AJ 《American journal of veterinary research》2012,73(5):689-697

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