Continuous lumbar hemilaminectomy for intervertebral disc disease in an Amur tiger (Panthera tigris altaica)     

Thomas Flegel  Peter B?ttcher  Michaele Alef  Ingmar Kiefer  Eberhard Ludewig  Jens Thielebein  Vera Grevel 《Journal of zoo and wildlife medicine》2008,39(3):468-471

A 13-yr-old Amur tiger (Panthera tigris altaica) was presented for an acute onset of paraplegia. Spinal imaging that included plain radiographs, myelography, and computed tomography performed under general anesthesia revealed lateralized spinal cord compression at the intervertebral disc space L4-5 caused by intervertebral disc extrusion. This extrusion was accompanied by an extensive epidural hemorrhage from L3 to L6. Therefore, a continuous hemilaminectomy from L3 to L6 was performed, resulting in complete decompression of the spinal cord. The tiger was ambulatory again 10 days after the surgery. This case suggests that the potential benefit of complete spinal cord decompression may outweigh the risk of causing clinically significant spinal instability after extensive decompression.  相似文献   

Reply to Letter to the Editor by Drs. Evett and Schwartz     

J. Vera 《Agricultural Water Management》2010,97(1):185

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Characterization of single‐nucleotide polymorphism markers in the Mediterranean mussel,Mytilus galloprovincialis     

Manuel Vera  Belén G Pardo  Ania Pino‐Querido  José Antonio Álvarez‐Dios  José Fuentes  Paulino Martínez 《Aquaculture Research》2010,41(10):e568-e575

The Mediterranean mussel, Mytilus galloprovincialis, is one of the most important aquaculture species in Europe. Appropriate molecular markers are required to evaluate genetic resources and to trace genealogies in breeding programmes for improving mussel culture. Microsatellites have been commonly applied to this purpose in other species. However, Mediterranean mussel microsatellites have demonstrated high frequencies of null alleles that hamper accurate estimates of population parameters and confident parentage inferences. As alternative markers, we have characterized in silico 25 potential single‐nucleotide polymorphism (SNP) markers in the Mediterranean mussel from expressed sequence tag (EST) public databases. The genotyping of SNPs was performed using a single‐base extension approach. Their polymorphism was evaluated in 47 individuals from an Atlantic population. Out of the 25 potential SNPs tested, 12 were technically feasible (producing a single amplicon) and polymorphic. All were biallelic and had an unbiased heterozygosity ranging from 0.160 to 0.504. One SNP was from a mitochondrial gene. The combined potential of nuclear SNPs for parentage assignment gave an exclusion probability of a false couple of parents of 0.9471. These markers will be useful for evaluating resources and tracing genealogies in genetic breeding programmes implemented to solve the main problems of mussel culture.  相似文献   

Combining ability of common bean parents in different seasons,locations and generations     

Lisandra Magna Moura  Rafael Silva Ramos dos Anjos  Renata Oliveira Batista  Naine Martins do Vale  Cosme Damião Cruz  José Eustáquio de Souza Carneiro  Juarez Campolina Machado  Pedro Crescêncio Souza Carneiro 《Euphytica》2018,214(10):181

The objectives of this study were to obtain information about genetic parameters of a partial diallel in common bean, as well as to evaluate the effects of growing seasons, locations and generations in these estimates in order to define the best strategies for the use of diallel analysis as methodology of selection of parents and populations in common bean breeding. Twelve parents were crossed in a partial diallel scheme (5?×?7), and the F₁ and F₂ generations were evaluated in four experiments (I, II, III, and IV). The effects of seasons were determined in the Experiments I and II, in which the F₁ generation was evaluated in two distinct growing seasons. To quantify the effect of locations, we used Experiments III and IV, in which the F₂ generation was evaluated in the same season, but at different locations, and to quantify the effect of generations, the experiments II (generation F₁) and III (F₂ generation) were evaluated in the same season and location. As for grain yield the genetic control varied according to the environment. An effect of interaction on the genetic parameters of the diallel was observed for generation, whereas for grain yield, effects of season and location were observed. For plant architecture, parental combining ability should therefore be estimated using the F₁ generation, while for grain yield, both the F₁ and F₂ can be used. The recombination between the best hybrids is a promising strategy for extraction of superior black bean lines.  相似文献   

Using humic products as amendments to restore Zn and Pb polluted soil: a case study using rapid screening phytotest endpoint     

Maria Pukalchik  Maria Panova  Mikhail Karpukhin  Olga Yakimenko  Kamila Kydralieva  Vera Terekhova 《Journal of Soils and Sediments》2018,18(3):750-761

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Masculinization of the Native Bay Snook,Petenia splendida,Using Oral Administration of the Synthetic Steroid 17α‐methyltestosterone          下载免费PDF全文

María de J. Contreras‐García  Wilfrido M. Contreras‐Sánchez  Alejandro Mcdonal‐Vera  Ulises Hernández‐Vidal 《Journal of the World Aquaculture Society》2018,49(5):889-896

The possibility of inducing sex reversal in bay snook, Petenia splendida, was evaluated to generate a protocol aimed at producing single‐sex populations (100% males) and improving farming conditions for this species. This popular staple food is a cichlid fish native to Central America, with aquaculture potential. We evaluated oral administration of the synthetic steroid 17α‐methyltestosterone (MT) to determine optimal dosage (30, 45, and 60 mg/kg of food) and days of feeding (30, 45, and 60) to attain efficient masculinization. The statistical analysis indicates a highly significant effect of both factors as well as for the interaction (P < 0.01). We registered significant differences in growth at the end of the experiment; fish fed with 60 mg of MT for 60 d reached the largest size, while fish fed with no MT were smaller. No significant differences were observed for survival (P > 0.05), ranging from 55.7 to 75.3% in the treatments. We recommend the use of 60 mg/kg of MT for 30 d for achieving successful sex reversal. This study provides valuable information supporting the inclusion of new species in aquaculture, reducing time and costs associated with sex reversal previously proposed for the species.  相似文献   

Genotyping of red and white fruited strawberry (Fragaria L.) accessions and hybrids based on microsatellite markers and on the genetic diversity in the allergen genes fra a 1 and fra a 3     

René Kaiser  Vera Mageney  Konrad Schwefel  Daniela Vollmers  Aenne Krüger  Renate Horn 《Genetic Resources and Crop Evolution》2016,63(7):1203-1217

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Tympanic ossicles and pharyngeal arches     

Whyte J  Cisneros A  Yus C  Fraile J  Obón J  Vera A 《Anatomia, histologia, embryologia》2009,38(1):31-33

We have performed a study on 11 human embryos regarding the development of the tympanic ossicles and their relationship with the first pharyngeal arch. After performing measurements to date the embryos and foetuses chronologically, we performed a meticulous dissection of the temporal bones. Subsequently, they were fixed in 10% formol, decalcified with 2% nitric acid, embedded in Paraplast, sectioned in 7-mm sequences and stained with Martin's trichrome technique. In the 21- and 24-mm cranium-raquis (CR) length human embryos, we have observed the head of the malleus and the body of the incus close to Meckel's cartilage, in addition to the handle of the malleus, the long limb of the incus and the stapes. Between them there was a mesenchymal band inside the primordium of the tympanic cavity. In the 27-mm CR embryo, the various components of the malleus and incus were fusing, and in the 30-mm CR embryo the union was complete. From our observations, we can conclude that the malleus and the incus are derived from the first and second pharyngeal arches.  相似文献   

Integrating the fish embryo toxicity test as triad element for sediment toxicity assessment based on the Water Framework Directive approach     

Mariana?BartzkeEmail author  Vera?Delov  Petra?Stahlschmidt-Allner  Bernhard?Allner  J?rg?Oehlmann 《Journal of Soils and Sediments》2010,10(3):389-399

Purpose  

The objective of this study was to complement analyses according to the European Union Water Framework Directive (WFD) with a sediment toxicity analysis as part of an integrated river assessment. To this end, Hessian water courses were analyzed using the sediment quality triad concept according to Chapman with chemical analyses, in situ effect evaluations, and ecotoxicological assessments. For the ecotoxicological assessment (fish embryo toxicity test with Danio rerio), a new evaluation scheme was developed, the fish teratogenicity index (FTI), that allows for a classification of sediments into ecological quality classes compliant to the WFD.  相似文献   

Fique fibers: Enhancement of the tensile strength of alkali treated fibers during tensile load application     

Catalina Gómez Hoyos  Vera A. Alvarez  Piedad Ga?án Rojo  Analia Vázquez 《Fibers and Polymers》2012,13(5):632-640

Fique fibers were treated using Na(OH) solution at 5 w/v%, slack and under 1 N of tension, at room temperature, for 4 and 24 h respectively. Changes in their structure and composition were monitored using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and X-ray diffraction (XRD). Additionally their mechanical properties were evaluated and analyzed. Results showed that tensile load application during alkali treatment improves their tensile strength and modulus. The most important change in mechanical properties was achieved in fibers treated for 24 h under tension. However, these fibers presented a high standard deviation; due to this treatment causing an important defibrillation. Moreover, fibers treated for 4 hours under tension, enhance their tensile strength around 56 %, while slack treated fibers improve only 38 %. When fibers were treated under tension, cellulose microfibrills were rearranged in the direction of tensile application and the spiral angle decreased, increasing the molecular orientation.  相似文献