Development of simplified and rapid nucleic acid release protocol for PCR based detection of <Emphasis Type="Italic">Potato viruses</Emphasis>     

Baswaraj Raigond  Ambika Verma  Tarvinder Kochhar  Shivani Roach  Sanjeev Sharma  S. K. Chakrabarti 《Phytoparasitica》2018,46(2):255-262

A simple, cost-effective and rapid viral nucleic acid release (NAR) buffer suitable for RT-PCR based diagnostic assay was developed for the detection of potato viruses. The NAR buffer and commercially available RNA isolation kit were compared for RT-PCR based assay, where an amplicon of expected size (~380 bp) targeting PVY was observed in both isolations indicating that it can be used in RT-PCR based diagnostic assays. The same was further validated for its repeatability by running across more than hundred suspected potato leaf samples collected from different sources where, it showed consistent results for the presence of PVY indicating its reliability. The NAR buffer assay was examined for its sensitivity in comparison with the kit based isolation where both the assays were able to detect even up to 10^?5 dilution without affecting the sensitivity. NAR buffer was found stable up to 28 days at -20 °C and for 14 days at 4 °C without losing PCR sensitivity. The assay was also found effective to release the nucleic acid from potato leaves, thrips and aphids for PCR and RT-PCR based detection of DNA viruses like ToLCNDV-potato and other RNA viruses. The developed protocol is simple, less laborious, time-saving (10-15 min) and economical (1/100th of kit) as compared to kit based protocol. The assay can be adopted in diagnostic laboratories for detection of RNA/DNA viruses from potato plants and in thrips as well.  相似文献   

Manure and Fertilizer Application Effects on Aggregate and Mineral-Associated Organic Carbon in a Loamy Soil under Rice-Wheat System     

Dinesh Kumar Benbi  Amardeep Singh Toor  Gayatri Verma 《Communications in Soil Science and Plant Analysis》2016,47(15):1828-1844

The study was conducted to investigate the aggregate stability and distribution of organic carbon (C) in different-sized aggregates and mineral fractions in a loamy soil under rice-wheat system with continuous application of fertilizer nitrogen, phosphorus and potassium (NPK), farmyard manure (FYM), N+FYM and NPK+FYM, compared with unfertilized control. Macro-aggregates comprised 37.1–49.3% of the total water-stable aggregates (WSAs), compared with 23.3–30.3% as micro-aggregates. Application of inorganic fertilizers and FYM significantly increased the proportion of macro-aggregates, which were linearly related to total organic C (TOC). Organic C concentration in coarse macro-aggregates (CMacA) was higher than the micro-aggregates (CMicA). Application of FYM significantly increased the concentration of organic C in different-sized aggregates and mineral fraction, compared with the unfertilized control. Averaged across treatments, mineral-associated C comprised 26% of TOC. Macro-aggregates, on an average, constituted 66–68% of C preservation capacity of WSA. The amount of TOC sequestered was higher when NPK+FYM was applied together.  相似文献   

Agrobacterium-mediated Cry1A(b) gene transfer in Punica granatum L. cv. Kandhari Kabuli using different In Vitro regeneration pathways     

Vipasha Verma  Kamlesh Kanwar  Mahak Tufchi  Monika Kashyap 《Journal of Crop Science and Biotechnology》2014,17(1):1-10

Three different regeneration systems, viz. regeneration through callus cultures using embryonic explant, direct regeneration using shoot bud/nodal segments as explant and regeneration through cell suspension culture using cotyledonary explant (for the induction of transgenic callus for suspension culture) were evaluated to see their effect on transfer of Cry1A(b) gene to Punica granatum L. cv. Kandhari Kabuli through Agrobacterium mediated transformation. Pre-conditioning and co-cultivation durations had a marked effect on transformation frequency of different explants. Out of different explants used (embryo, shoot bud, and cotyledon) for different regeneration systems cotyledonary explant showed highest putative transformation frequency (13.54%) inducing callus on selective medium for carrying out cell suspension culture to regenerate transgenic shoots. Despite of the highest transformation frequency obtained from the cotyledon explant, the plating efficiency of the transgenic cells generated through the transgenic callus (callus formed from the cotyledonary explant) during cell suspension culture was found to be very low (0.7%). Thus the plating efficiency has also played worth mentioning role in the regeneration of transformants following cell suspension culture. Among the three regeneration systems, regeneration through callus cultures using embryonic explant was found to be best for regeneration of transformants. The highest per cent regeneration of 23.33 was obtained from the putative transgenic embrogenic calli. Successful genetic transformation in the transformed plantlets was confirmed by PCR analysis. The transformation system thus developed is valuable and may be used to produce insect resistant trees.  相似文献   

Induction of oxidative stress and lipid peroxidation in rats chronically exposed to cypermethrin through dermal application     

Rajinder Raina  Pawan K. Verma  N. K. Pankaj  Shahid Prawez 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):257-259

Present study was undertaken to study the effect of cypermethrin on oxidative stress after chronic dermal application. The insecticide was applied dermally at 50 mg/kg body weight in different groups of Wistar rats of either sex weighing 150~200 g. Significant (p < 0.05) increase in catalase activity was observed after 30 days of exposure. However, the superoxide dismutase activity declined significantly after 60 days of exposure. The activity of glutathione peroxidase and blood glutathione levels declined significantly (p < 0.05) after 30 days of cypermethrin dermal application. However, the activity of glutathione S-transferase increased significantly (p < 0.05) in all groups after 60 days of dermal exposure. Significant increase in lipid peroxidation was observed from 30 days onwards and reached a peak after 120 days of application.  相似文献   

Estimation of phenotypic divergence in a collection of <Emphasis Type="Italic">Capsicum</Emphasis> species for yield-related traits     

S. T. Thul  R. K. Lal  A. K. Shasany  M. P. Darokar  A. K. Gupta  M. M. Gupta  R. K. Verma  S. P. S. Khanuja 《Euphytica》2009,168(2):189-196

Phenotypic divergence among the 24 accessions belonging to a collection of six species of Capsicum from different geoclimatic regions available in field gene bank of CIMAP, Lucknow, India, was quantified by multivariate analysis for 12 quantitative and qualitative traits. Based on their values, all 24 accessions were grouped into six clusters such that the genetic stocks within cluster had smaller D ² values among themselves than those belonging to different clusters. The accessions of Capsicum annuum were distributed in different clusters in morphotyping representing different species, suggesting that taxonomic characteristics are not always related to agronomic traits. No parallelism/association was found between geographical and phenotypic diversity. Accessions 15 and 23 (clusters V and VI, respectively) had distinct identity. The three characteristics that played the greatest role in differentiation were fruit diameter, number of fruits per plant, and leaf diameter, which can be utilized as conventional/morphological markers for the improvement of chilli yield and obtaining good segregants in chilli breeding programs.  相似文献   

Estimating seasonal evapotranspiration from temporal satellite images   总被引：1，自引：1，他引：0  

Ramesh K. Singh  Shuguang Liu  Larry L. Tieszen  Andrew E. Suyker  Shashi B. Verma 《Irrigation Science》2012,30(4):303-313

Estimating seasonal evapotranspiration (ET) has many applications in water resources planning and management, including hydrological and ecological modeling. Availability of satellite remote sensing images is limited due to repeat cycle of satellite or cloud cover. This study was conducted to determine the suitability of different methods namely cubic spline, fixed, and linear for estimating seasonal ET from temporal remotely sensed images. Mapping Evapotranspiration at high Resolution with Internalized Calibration (METRIC) model in conjunction with the wet METRIC (wMETRIC), a modified version of the METRIC model, was used to estimate ET on the days of satellite overpass using eight Landsat images during the 2001 crop growing season in Midwest USA. The model-estimated daily ET was in good agreement (R ²?=?0.91) with the eddy covariance tower-measured daily ET. The standard error of daily ET was 0.6?mm (20%) at three validation sites in Nebraska, USA. There was no statistically significant difference (P?>?0.05) among the cubic spline, fixed, and linear methods for computing seasonal (July–December) ET from temporal ET estimates. Overall, the cubic spline resulted in the lowest standard error of 6?mm (1.67%) for seasonal ET. However, further testing of this method for multiple years is necessary to determine its suitability.  相似文献   

Aroma profiles of the curry leaf, Murraya koenigii (L.) Spreng. chemotypes: Variability in north India during the year     

Ram Swaroop Verma  Rajendra Chandra PadaliaVinod Arya  Amit Chauhan 《Industrial Crops and Products》2012,36(1):343-348

Murraya koenigii (L.) Spreng. (Rutaceae) is characterized by a large chemical intraspecific variability among the land races. This fact makes it difficult to detect real changes occurring in their essential oil composition during annual cycle. Based on this, variations of essential oil yield and composition in two chemotypes (‘A’ and ‘B’) of M. koenigii were assessed in spring, summer, rainy, autumn and winter seasons under foot hill conditions of northern India. The essential oil yield ranged from 0.15% to 0.18% in chemotype ‘A’, while it varied from 0.12% to 0.14% in chemotype ‘B’. Essential oils of both chemotypes from different seasons were analysed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of fifty-eight constituents representing 93.7-98.8% of chemotype ‘A’ and fifty-six constituents forming 96.1-98.7% of the total composition of chemotype ‘B’ were identified. Chemotype ‘A’ was characterized by higher percentages of α-pinene (34.6-41.9%), sabinene (26.1-36.1%), (E)-caryophyllene (2.4-5.4%) and terpinen-4-ol (1.5-5.3%), whereas chemotype ‘B’ was dominated by higher amount of α-pinene (52.7-65.3%), β-pinene (10.7-12.9%), (E)-caryophyllene (3.1-10.3%) and limonene (5.1-7.8%). Comparative results showed considerable variations in the essential oil composition of both chemotypes due to season of collection. Present study concluded that the M. koenigii leaves of desired quality may be obtained by selecting suitable chemotype and season.  相似文献   

Phenolic Content and Antioxidant Properties of Bran in 51 Wheat Cultivars     

Brij Verma  Pierre. Hucl  Ravindra N. Chibbar 《Cereal Chemistry》2008,85(4):544-549

Whole‐grain‐based diets have been suggested to reduce the incidence of cardiovascular disease and colon cancer. Phenolic compounds, most of which are present in the wheat bran, may be one of the factors contributing to whole‐grain health benefits. We measured the free, bound, and total phenolic content and antioxidant activity in the bran of 51 wheat cultivars belonging to eight Western Canadian spring wheat market classes grown in a replicated trial at Saskatoon, Saskatchewan, Canada. The free phenolic (extracted with 80% v/v aqueous ethanol) content ranged from 854.1 ± 265.1 to 1,754.9 ± 240.3 μg/g of bran gallic acid equivalent (GAE). Saponification followed by a liquid‐liquid solvent extraction released bound phenols ranging from 2,304.9 ± 483.0 to 5,386.1 ± 927.5 μg/g of bran GAE, contributing 66–82% of the total wheat bran phenolic content. Total phenolic content ranged from 3,406.4 ± 32.3 to 6,702.7 ± 19.6 μg/g of bran GAE, with the average being 5,197.2 ± 804.9 μg/g of bran GAE. Antioxidant activity ranged from 11.86 ± 2.59 to 20.12 ± 0.51%, while the overall average was 15.6 ± 2.2%. Based on varietal means, antioxidant activity correlated with free, bound, and total phenolic content (r = 0.8, P < 0.05).  相似文献   

Comparative Germplasm Characterization of Maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) in Rajouri Region of Pir Panjal Himalaya J &amp; K (India), based on Morphological and ISSR Markers     

Tanvir H. Dar  Rubiada Shakeel  Shusheel Verma 《Journal of Crop Science and Biotechnology》2018,21(1):43-55

  相似文献   

Effect of organic and inorganic sources of nutrients on soil microbial activity and soil organic carbon build-up under rice in west coast of India     

Mahajan Gopal Ramdas  Manjunath B. L.  Singh Narendra Pratap  Ramesh R  Verma R. R  Latare Ashish Marutrao 《Archives of Agronomy and Soil Science》2017,63(3):414-426

The effect of medium-term (5 years) application of organic and inorganic sources of nutrients (as mineral or inorganic fertilizers) on soil organic carbon (SOC), SOC stock, carbon (C) build-up rate, microbial and enzyme activities in flooded rice soils was tested in west coast of India. Compared to the application of vermicompost, glyricidia (Glyricidia maculate) (fresh) and eupatorium (Chromolaena adenophorum) (fresh) and dhaincha (Sesbania rostrata) (fresh), the application of farmyard manure (FYM) and combined application of paddy straw (dry) and water hyacinth (PsWh) (fresh) improved the SOC content significantly (p < 0.05). The lowest (p < 0.05) SOC content (0.81%) was observed in untreated control. The highest (p < 0.05) SOC stock (23.7 Mg C ha^?1) was observed in FYM-treated plots followed by recommended dose of mineral fertilizer (RDF) (23.2 Mg C ha^?1) and it was lowest (16.5 Mg C ha^?1) in untreated control. Soil microbial biomass carbon (C_mb) (246 µg g^?1 soil) and C_mb/SOC (1.92%) were highest (p < 0.05) in FYM-treated plot. The highest (p < 0.05) value of metabolic quotient (qCO₂) was recorded under RDF (19.7 µg CO₂-C g^?1 C_mb h^?1) and untreated control (19.6 µg CO₂-C g^?1 C_mb h^?1). Application of organic and inorganic sources of nutrients impacted soil enzyme activities significantly (p < 0.05) with FYM causing highest dehydrogenase (20.5 µg TPF g^?1 day^?1), phosphatase (659 µg PNP g^?1 h^?1) and urease (0.29 µg urea g^?1 h^?1) activities. Application of organic source of nutrients especially FYM improved the microbial and enzyme activities in flooded and transplanted rice soils. Although the grain yield was higher with the application of RDF, but the use of FYM as an organic agricultural practice is more useful when efforts are intended to conserve more SOC and improved microbial activity.  相似文献