Application of platelet-rich plasma in the in vitro production of bovine embryos     

Ramos-Deus  Pamela  Santos Nascimento  Pábola  Vieira  Joane Isis Travassos  Chaves  Maiana Silva  Albuquerque  Karoline Antunes  Ferreira-Silva  José Carlos  Grázia  João Grabriel Viana  Santos Filho  Antonio Santana  Batista  André Mariano  Teixeira  Valéria Wanderley  Oliveira  Marcos Antonio Lemos 《Tropical animal health and production》2020,52(6):2931-2936

Tropical Animal Health and Production - The aim of this study was to replace fetal bovine serum (FBS) with platelet-rich plasma (PRP) for in vitro production of bovine embryos. The maturation media...  相似文献   

Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil     

de Jesús Pires  Carolline  Costa  Marcones Ferreira  Zucchi  Maria Imaculada  Ferreira-Gomes  Regina Lucia  Pinheiro  José Baldin  Viana  João Paulo Gomes  Bajay  Miklos Maximiliano  Assunção-Filho  José Ribamar  de Almeida Lopes  Ângela Celis 《Genetic Resources and Crop Evolution》2022,69(3):973-986

Genetic Resources and Crop Evolution - Lima bean (Phaseolus lunatus L.) have high genetic diversity, especially in Brazil, which has been maintained both on farms and in germplasm banks. Knowledge...  相似文献   

Genetic parameters relating isoflavone and protein content in soybean seeds   总被引：3，自引：0，他引：3  

Lucimara Chiari  Newton Deniz Piovesan  Lucas Koshy Naoe  Inês Chamel José  José Marcelo Soriano Viana  Maurilio Alves Moreira  Everaldo Gonçalves de Barros 《Euphytica》2004,138(1):55-60

Isoflavones are a class of compounds present in high amounts in soybean seeds, which can be used for prevention and treatment of several chronic diseases. Proteins present in soybean seeds are the basis for the high nutritional value and versatility of this leguminous species in animal and human feeding. The main goals of this work were to estimate heritabilities for isoflavone contents in soybean seeds and the correlation between isoflavone and protein contents. Commercial variety IAC-100 (high isoflavone and normal protein contents) and the line BARC-8 (low isoflavone and high protein contents) were crossed, and one single F₁ plant derived 97 F₂ seeds, which were used to obtain F₃ seeds. A sample of F₃ seeds from each F₂ plant was used for isoflavone determination by HPLC and protein by the Kjeldahl method. Six isoflavone forms were detected: daidzin, genistin, glycitin, malonyldaidzin, malonylgenistin and malonylglycitin. Total isoflavone contents ranged from 427.92 to 965.89 μg per gram of dry seed and the protein content ranged from 45.17 to 34.95% in BARC-8 and IAC-100, respectively. Our results indicate that it is possible to select for high isoflavone content in early breeding generations because the broad sense heritabilities for the contents of the various isoflavone forms were higher than 90%. In addition, high correlation values among the contents of the individual isoflavone forms were observed (between 0.80 and 0.98). However, negative correlation values were obtained between isoflavone and protein contents, ranging from −0.51 to −0.37 for the different isoflavone forms. The correlation value of −0.47 between total isoflavone and protein contents confirmed the negative correlation between these two parameters, as reported by other authors. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Inferring tropical popcorn gene pools based on molecular and phenotypic data     

Gabriel Borges Mundim  José Marcelo Soriano Viana  Ciro Maia  Geísa Pinheiro Paes  Rodrigo Oliveira DeLima  Mágno Sávio Ferreira Valente 《Euphytica》2015,202(1):55-68

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短尾鮠精子细胞和精子的超微结构研究     

张汉峰  魏刚  黄林  代大临  陈永强  张运明 《西南大学学报(自然科学版)》2005,27(5):696-699

用透射电镜观察了短尾鮠精子细胞和精子的形态以及细胞核、拟染色体和线粒体等的结构变化规律。结果表明,随着精子细胞的发育,核质凝聚程度逐渐增强。精子细胞时期细胞器较丰富,到精子细胞后期,细胞器的形态和数量发生了较大变化。短尾鮠的精子具有椭圆的头部和复杂的中片;近侧中心粒和远侧中心粒靠近核的中央;鞭毛呈9+2轴丝结构,并具有由外膜折迭形成的波浪形的鳍状结构。  相似文献   

Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula     

Costa LS  Fidelis GP  Telles CB  Dantas-Santos N  Camara RB  Cordeiro SL  Costa MS  Almeida-Lima J  Melo-Silveira RF  Oliveira RM  Albuquerque IR  Andrade GP  Rocha HA 《Marine drugs》2011,9(6):952-966

Fucan is a term used to denominate a type of polysaccharide which contains substantial percentages of l-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed mainly of fucose, glucose, glucuronic acid, galactose and sulfate. These fucans did not show anticoagulant activity in PT and aPTT tests. Their antioxidant activity was evaluated using the follow tests; total antioxidant capacity, scavenging hydroxyl and superoxide radicals, reducing power and ferrous ion [Fe(II)] chelating. All heterofucans displayed considerable activity, especially SF-1.0v which showed the most significant antioxidant potential with 90.7 ascorbic acid equivalents in a total antioxidant capacity test and similar activity when compared with vitamin C in a reducing power assay. The fucan antiproliferative activity was performed with HeLa, PC3 and HepG2 cells using MTT test. In all tested conditions the heterofucans exhibited a dose-dependent effect. The strongest inhibition was observed in HeLa cells, where SF-1.0 and SF-1.5 exhibited considerable activity with an IC50 value of 15.69 and 13.83 μM, respectively. These results clearly indicate the beneficial effect of S. filipendula polysaccharides as antiproliferative and antioxidant. Further purification steps and additional studies on structural features as well as in vivo experiments are needed to test the viability of their use as therapeutic agents.  相似文献   

A health evaluation in a colony of captive collared peccaries (Tayassu tajacu) in the Eastern Amazon     

Mayor P  Le Pendu Y  Guimarães DA  da Silva JV  Tavares HL  Tello M  Pereira W  López-Béjar M  Jori F 《Research in veterinary science》2006,81(2):246-253

This study pretends to determine baseline data on the health and mortality of a colony of captive collared peccaries in the Eastern Amazon (Belém, State of Pará, Brazil) during a 65-months survey. Thirty-nine out of 166 animals (23.5%) died and were examined post-mortem. Monthly mortality averaged 1.2%. The highest mortality rate was observed in newborns (74.4%). Abandonment by the mother and aggression were responsible for 24.1% and 13.8% of the total newborn deaths, respectively. Most frequent causes of non-neonatal death were food poisoning (50.0%) due to an episode of accidental bitter cassava leaves ingestion and traumatism due to aggressions between animals (10.0%). Results from serology for different infectious diseases showed that 4.9% (2/41) collared peccaries had antibodies against Brucella spp. and 9.8% (4/41) animals had antibodies to two different Leptospira spp. serovars, butembo and autumnalis. This is the first survey of morbidity and mortality in captive collared peccaries in the Amazon region.  相似文献