Analysis of the spatial variation of Bovine tuberculosis disease risk in Spain (2006-2009)     

Allepuz A  Casal J  Napp S  Saez M  Alba A  Vilar M  Domingo M  González MA  Duran-Ferrer M  Vicente J  Alvarez J  Muñoz M  Saez JL 《Preventive veterinary medicine》2011,100(1):441-52

In this study we explored the spatial variation of Bovine tuberculosis (BTB) risk of being positive, new positive or persistently positive, as well as the risk of eliminating BTB in positive herds throughout Spain from 2006 to 2009 by means of hierarchical Bayesian models. The results of the models showed that the risk of infection (positive or new positive herds), persistence and elimination was lower in counties located in north and north-eastern of Spain, and in the Balearic and Canary islands than in the rest of the country. In some counties the risk of positivity was high during the four years of study, whereas there were others where the risk of positivity was high only in some of the years. With regard to the risk of persistence of BTB positive herds, counties located in the central, western and south-western part of the country had a higher risk in the three studied periods. This study has identified some specific areas of increased BTB risk in Spain, information that is useful for disease management.  相似文献   

Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog     

de Miranda RL  de Castro JR  Olegário MM  Beletti ME  Mundim AV  O'Dwyer LH  Eyal O  Talmi-Frank D  Cury MC  Baneth G 《Veterinary parasitology》2011,177(3-4):392-396

Canine hepatozoonosis is a tick-borne disease caused by protozoans of the genus Hepatozoon. Several tick species have been implicated as potential vectors. Therefore, extensive studies are needed to determine the 'natural' endemic cycle of this parasite. This paper presents the first report of the presence of Hepatozoon canis oocysts in Rhipicephalus (Boophilus) microplus collected from an infected dog.  相似文献   

Use of crude, FML and rK39 antigens in ELISA to detect anti-Leishmania spp. antibodies in Felis catus     

da Silveira Neto L  Sobrinho LS  Martins CO  Machado RZ  Marcondes M  de Lima VM 《Veterinary parasitology》2011,177(3-4):374-377

Visceral leishmaniasis is a disease caused by Leishmania (Leishmania) chagasi and represents a serious public health problem. The dog is the main urban reservoir of the disease; however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. This study aimed to detect cats seropositive for Leishmania spp. using different antigens. Additional studies were performed using sera from cats with Toxoplasma gondii (n=15) to evaluate cross-reactivity. Serum samples (n=113) from cats living in the town of Ara?atuba, State of S?o Paulo, Brazil, an endemic area for human and canine visceral leishmaniasis, were tested by indirect ELISA using different antigens: crude (CAG-ELISA), fucose-mannose ligand (FML-ELISA) and K39 (rK39-ELISA). Anti-Leishmania spp. antibodies were detected in 23.0% of samples evaluated by CAG-ELISA, 13.3% by FML-ELISA and 15.9% by RK39-ELISA. Only reactive sera in all three tests were considered truly positive. No disagreement occurred among the tests (p<0.05). Serum samples seropositive for toxoplasmosis tested by CAG-ELISA were negative, but one sample (6.7%) was positive for FML-ELISA and rK39-ELISA suggesting a cross-reaction between these antigens and anti-T. gondii antibodies. These findings indicate the occurrence of feline leishmaniasis in Ara?atuba. Further studies are required to clarify the role of cats in the epidemiological cycle of leishmaniasis.  相似文献   

Genetic analysis of Anisakis typica (Nematoda: Anisakidae) from cetaceans of the northeast coast of Brazil: new data on its definitive hosts     

Iñiguez AM  Carvalho VL  Motta MR  Pinheiro DC  Vicente AC 《Veterinary parasitology》2011,175(3-4):293-299

Prevalence and seasonal variations of helminth infections and their association with morbidity parameters were studied in traditionally reared Cambodian cattle. Four villages in two provinces of West Cambodia were visited on monthly intervals over a period of 11 months, during which 2391 animals were faecal and blood sampled for parasitological and haematological examinations. The body condition score (BCS), faecal consistency (diarrhoea score, DS), colour of the ocular conjunctivae (FAMACHA(?)) and packed cell volume were determined for each individual animal. The overall proportion of samples that was positive for gastrointestinal nematodes was 52%, 44% and 37% in calves (from 1 to 6 months), young animals (6 to 24 months) and adults (over 24 months), respectively, while geometric mean faecal egg counts (FECs) for each of these age categories were 125, 66 and 15 eggs per gram, respectively. Six genera of strongyles were found in the faecal cultures, i.e. in descending order of occurrence, Cooperia, Oesophagostomum, Haemonchus, Trichostrongylus, Mecistocirrus and Bunostomum. The prevalences of Fasciola and Paramphistomum, estimated by coprological examination, varied between 5-20% and 45-95%, respectively. Logistic mixed models were used to investigate associations of morbidity markers with the presence of parasite infection. A low BCS was associated with gastrointestinal nematode and liver fluke infections, and soft faecal consistency with Paramphistomum infections. However, other factors such as nutritional deficiencies and intercurrent diseases are likely to enhance the effects of parasites and should therefore be considered when using these morbidity parameters as indicators of parasitism.  相似文献   

Leishmania chagasi infection in cats with dermatologic lesions from an endemic area of visceral leishmaniosis in Brazil     

Vides JP  Schwardt TF  Sobrinho LS  Marinho M  Laurenti MD  Biondo AW  Leutenegger C  Marcondes M 《Veterinary parasitology》2011,178(1-2):22-28

Although dogs are considered the main domestic reservoirs for Visceral Leishmaniosis (VL), which is caused in the Americas by Leishmania chagasi, infected cats have also been recently found in endemic areas of several countries and became a public health concern. Accordingly, the purpose of this study was to evaluate cats with dermatologic lesions from an endemic area of VL and the natural infection of L. chagasi. A total of 55 cats were selected between April 2008 and November 2009 from two major animal shelters of Ara?atuba, Southeastern Brazil. All cats underwent general and dermatologic examinations, followed by direct parasitological examination of lymphoid organs, immunosorbent assay (ELISA) and indirect immunofluorescence (IFAT). In addition, detection of amastigotes was performed by immunohistochemistry (IHC) in skin lesions of all cats. VL was diagnosed in 27/55 (49.1%) cats with dermatological problems. Amastigotes were found in lymphoid organs of 10/27 (37.0%) cats; serology of 14/27 (51.9%), 6/27 (22.2%) and 5/27 (18.5%) cats was positive for ELISA, IFAT and both, respectively. The IHC identified 9/27 (33.3%) cats; 5/27 (18.5%) were positive only for IHC and therefore increased the overall sensitivity. Specific FIV antibodies were found in 6/55 (10.9%) cats, of which 5/6 (83.3%) had leishmaniosis. Real time PCR followed by amplicon sequencing successfully confirmed L. chagasi infection. In conclusion, dermatological lesions in cats from endemic areas was highly associated to visceral leishmaniosis, and therefore skin IHC and differential diagnosis of LV should be always conducted in dermatological patients in such areas.  相似文献   

Combinations of cactus pear with different roughage sources on the production,chemical composition,and milk fatty acid profile of F1 Holstein/Zebu cows     

Soares  Camila  Rocha Júnior  Vicente Ribeiro  Monção  Flávio Pinto  Borges  Lucas Daniel Alcântara  Caldeira  Luciana Albuquerque  Costa  Natanael Mendes  Ruas  José Reinaldo Mendes  Rigueira  João Paulo Sampaio  da Cunha Siqueira Carvalho  Cinara  de Sales  Eleuza Clarete Junqueira  de Assis Pires  Daniel Ananias  Chamone  Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576

Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can...  相似文献   

Mating Disruption of Striped Rice Stem Borer: Importance of Early Deployment of Dispensers and Impact on Airborne Pheromone Concentration     

Gavara Aitor  Vacas Sandra  Primo Jaime  Navarro-Llopis Vicente 《水稻科学》2021,28(6):525

正Mating disruption(MD) is an effective environmentallyfriendly control method against the striped rice stem borer(RSB), Chilo suppressalis(Lepidoptera: Crambidae). In this study, the current MD dispensers release the pheromone exponentially, with higher initial release rates that decrease throughout the time. To adjust the timing of dispensers' deployment and evaluate the importance of controlling the early first or the second male flight,  相似文献   

Changes in amino acid and organic acid composition in tomato and cucumber plants in relation to salinity and nitrogen nutrition     

Vicente Martinez  J. Maria Nuñez  Antonio Ortiz  Antonio Cerda 《Journal of plant nutrition》2013,36(8):1359-1368

Under conditions of salt stress, plants show qualitative and quantitative alterations in various organic compounds, such as nitrogen (N) compounds and organic acids. In this work, the effect of different saline levels as well as various N levels, supplied as nitrate (NO₃) or as ammonium (NH₄)+NO₃ on the concentration of amino acids and organic acids in the leaves of tomato and cucumber plants has been studied. The effect of the source of N on individual amino acid contents varied with plant species. Most of the amino acids increased when the concentration of N in the nutrient solution was increased, except when N was added as NH₄+NO₃ for tomato. The effect of salt stress depended on which amino acid was considered. The data also indicate that the effect of salinity on each particular amino acid was greatly dependent on the plant species and N source. Organic acids were differently affected by salinity and by the N source, depending on the plant species. In tomato, the concentrations of short‐chain organic acids were 2–3 times higher in NO₃‐supplied plants than in those grown with NH₄+NO₃. Finally, in cucumber, malic acid concentration increased as a function of the saline level in the medium.  相似文献   

Characterizing the Anti-HIV Activity of Papuamide A          下载免费PDF全文

Cynthia D Andjelic  Vicente Planelles  Louis R Barrows 《Marine drugs》2008,6(4):528-549

Papuamide A is representative of a class of marine derived cyclic depsipeptides, reported to have cytoprotective activity against HIV-1 in vitro. We show here that papuamide A acts as an entry inhibitor, preventing human immunodeficiency virus infection of host cells and that this inhibition is not specific to R5 or X4 tropic virus. This inhibition of viral entry was determined to not be due to papuamide A binding to CD4 or HIV gp120, the two proteins involved in the cell-virus recognition and binding. Furthermore, papuamide A was able to inhibit HIV pseudotype viruses expressing envelope glycoproteins from vesicular stomatitis virus or amphotropic murine leukemia virus indicating the mechanism of viral entry inhibition is not HIV-1 envelope glycoprotein specific. Time delayed addition studies with the pseudotyped viruses show that papuamide A inhibits viral infection only at the initial stage of the viral life cycle. Additionally, pretreatment studies revealed that the virus, and not the cell, is the target of papuamide A’s action. Together, these results suggest a direct virucidal mechanism of HIV-1 inhibition by papuamide A. We also demonstrate here that the other papuamides (B-D) are able to inhibit viral entry indicating that the free amino moiety of 2,3-diaminobutanoic acid residue is not required for the virucidal activity.  相似文献   

Large-scale structural variation and long-term growth dynamics of Juniperus thurifera trees in a managed woodland in Soria, central Spain     

Vicente Rozas  José Miguel Olano  Lucía DeSoto  David Bartolomé 《Annals of Forest Science》2008,65(8):809-809

? Juniper woodlands have a great conservational and productive importance in Spain. However, basic traits of their structural variation and growth dynamics are unknown.

? To characterize the structural variation and assess both height and radial growth patterns, we sampled and measured stem cross-sections every 0.5 m in height from 107 Spanish juniper (Juniperus thurifera L.) trees, which were selected based on a 350 m regular grid over a whole woodland of 3 300 ha in area. We used dendroecological techniques and spatial analysis to study tree structure and growth.

? Structural traits such as bole diameter and height were mainly determined by tree life-related parameters such as tree age and growth rate variation, while abiotic factors such as altitude played a minor role in determining structural variation. Over the last 300 years, juniper establishment has been continuous in time but discontinuous in space. Large-scale spatial heterogeneity of tree establishment, and the presence of an early growth suppression and abrupt growth changes in junipers > 100 years old are consequences of an intense management that almost ceased in the late 19th century.

? Abrupt growth releases and suppressions were synchronic among different age classes, and coincided with documentary records on livestock decrease and key historical changes in land use. Growth patterns suggest that juniper is a brow sing-tolerant species that is able to survive large periods of intense browsing pressure.

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