Black yeast-like fungi associated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae)     

Vicente VA  Orélis-Ribeiro R  Najafzadeh MJ  Sun J  Guerra RS  Miesch S  Ostrensky A  Meis JF  Klaassen CH  de Hoog GS  Boeger WA 《Veterinary microbiology》2012,158(1-2):109-122

Lethargic Crab Disease (LCD) caused extensive epizootic mortality of the mangrove land crab Ucides cordatus (Brachyura: Ocypodidae) along the Brazilian coast, mainly in the Northeastern region. The disease was named after the symptoms of slow movement of infected crabs. Causative agents were suspected to be two black yeast-like fungi of the family Herpotrichiellaceae (ascomycete order Chaetothyriales), judged by infected tissue biopsies from moribund U. cordatus. The aim of the present study is to prove that two species are involved in the disease: the recently described black yeast Exophiala cancerae, but also a less virulent, hitherto undescribed fonsecaea-like species, introduced here as the novel species Fonsecaea brasiliensis. Strains were identified by ITS rDNA sequencing, and species borderlines were established by multilocus sequencing and AFLP analysis. Fonsecaea brasiliensis proved to be closely related to the pathogenic species Cladophialophora devriesii which originally was isolated from a systemic infection in a human patient. The virulence of F. brasiliensis is lower than that of E. cancerae, as established by artificial inoculation of mangrove crabs.  相似文献   

Some pharmacokinetic parameters of ampicillin/sulbactam combination after intravenous and intramuscular administration to goats     

A. Espuny  C.M. Carceles  M.S. Vicente  E. Escudero 《The Veterinary quarterly》2013,33(4):136-140

Summary

Some pharmacokinetic parameters of an ampicillin/sulbactam (2:1) combination were studied in six goats, after intravenous and intramuscular injection at a single dosage of 20 mg/kg bodyweight (13.33 mg/kg of sodium ampicillin and 6.67 mg/kg of sodium sulbactam). The drugs were distributed according to an open two‐compartment model. The apparent volumes of distribution calculated by the area method of ampicillin and sulbactam were 0.34 ± 0.04 l/kg and 0.45 ± 0.15 1/kg, respectively, and the total body clearances were 0.72 ± 0.11 and 0.38 ± 0.07 l/kg.h. The half‐lives of ampicillin after intravenous and intramuscular administration were 0.32 ± 0.04 h and 0.71 ± 0.14 h, respectively. For sulbactam the half‐lives were 0.79 ± 0.18 h and 1.13 ± 0.21 h after administration by the same routes. The bioavailability after intramuscular injection was high and similar for both drugs (98,29% for ampicillin and 101.84% for sulbactam). The mean peak plasma levels of ampicillin (0.43 ± 0.27 h) and sulbactam (0.34 ± 0.14 h) were reached at a similar time, and peak concentrations were also similar and non‐proportional to the dose of the products administered (11.02 ± 3.11 mg/l of ampicillin and 9.5 ± 0.98 mg/l of sulbactam).  相似文献   

Pharmacokinetics of amoxicillin/clavulanic acid combination after intravenous and oral administration in goats     

C.M. Carceles  E. Escudero  M.S. Vicente  J.M. Serrano  S. Carli 《The Veterinary quarterly》2013,33(4):134-138

Summary

The intravenous and oral pharmacokinetics of an amoxicillin and clavulanic acid combination (20 mg/kg of sodium amoxicillin and 5 mg/kg of potassium clavulanate) were studied in six goats. After intravenous administration the pharmacokinetics of both drugs could be described by an open two‐compartment model. Amoxicillin had a greater distribution volume (0.19 ± 0.01 l/kg) than clavulanic acid (0.15 ± 0.01 l/kg), whereas the distribution and elimination constants were higher for the latter, which was eliminated more quickly than amoxicillin. After oral administration of both drugs their pharmacokinetic behaviour was best described by an open one‐compartment model with first‐order absorption. Elimination half‐lives were twice as long after oral (2.15 ± 0.20 h and 1.94 ± 0.16 h for amoxicillin and clavulanic acid respectively) than after intravenous administration (1.20 ± 0.16 h and 0.86 ± 0.09, respectively). An apparent ‘flip‐flop’ situation was evident in this study. Bioavailability was 27% for amoxicillin and 50% for clavulanic acid.  相似文献   

Distribution of PDIA3 transcript and protein in rat testis and sperm cells     

ML Wang  WL Guo  J Liu  L Li  WJ Xing 《Reproduction in domestic animals》2013,48(1):59-63

  相似文献   

Altrenogest Treatment Associated with a Farrowing Induction Protocol to Avoid Early Parturition in Sows     

TS Gaggini  J Perin  LS Arend  ML Bernardi  I Wentz  FP Bortolozzo 《Reproduction in domestic animals》2013,48(3):390-395

This study investigated the effect of altrenogest treatment on the farrowing development of sows, and birth weight (BW) and piglet survival until the third day of life. Three control groups were used: (i) sows that farrowed spontaneously before 114 day of gestation (CONT <114); (ii) sows that spontaneously farrowed at ≥114 day of gestation (CONT ≥114); (iii) sows that farrowed at ≥114 day with cloprostenol treatment (CONTCLOPR). Other sows were treated with altrenogest (Regumate^®) for 3 days (days 111, 112 and 113 of gestation): one group gave birth spontaneously (ALT) and the other group received altrenogest until day 113 and cloprostenol on day 114 (ALTCLOPR). There were no differences (p > 0.05) in farrowing duration, BW, coefficient of variation (CV) of BW, stillborn piglets, mummified foetuses, percentage of light piglets and survival until Day 3 between sows with and without cloprostenol treatment, in both control (CONT ≥114 vs CONTCLOPR) and altrenogest‐treated sows (ALT vs ALTCLOPR). Further comparisons were performed taking into account three groups: sows with early delivery (CONT <114 – farrowing before 114 days of gestation; n = 56), sows with longer gestation (CONT ≥114 – with and without cloprostenol treatment sows; n = 103) and ALT sows (with and without cloprostenol treatment; n = 105). Gestation length of CONT ≥114 and ALT sows was similar (p > 0.05), but higher than in CONT <114 sows. There were no differences (p > 0.05) between groups in farrowing duration, CV of BW, and percentages of stillborn piglets and mummified foetuses. Sows of CONT <114 group had a larger litter size and a lower BW than sows of the other two groups (p < 0.05). Sows of CONT <114 group had a higher percentage of lighter piglets and a lower piglet survival rate (p < 0.05) than ALT sows. In conclusion, altrenogest treatment proved to be an efficient method to avoid early parturition in 3–5 parity sows resulting in heavier piglets at birth.  相似文献   

Pseudomonas associated with Bursaphelenchus xylophilus,its insect vector and the host tree: A role in pine wilt disease?     

Marta Salgueiro Alves  Anabela Pereira  Cludia Vicente  Manuel Mota  Isabel Henriques 《Forest Pathology》2019,49(6)

In this study, we characterized the diversity of Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector (Monochamus galloprovincialis) and its host (Pinus pinaster), by a culture‐independent approach using rpoD clone libraries. Clone libraries of Pseudomonas rpoD were obtained from B. xylophilus, M. galloprovincialis and infected P. pinaster. Most M. galloprovincialis and B. xylophilus sequences grouped together in the P. fluorescens group. Genes related to xenobiotics degradation and phenylacetate synthesis were present in the genomes of the type strains closely related to sequences retrieved from the nematode libraries. Results demonstrated that the nematode, during its life stages inside the tree, maintains a diverse Pseudomonas community that is closely related to the one associated with the insect vector. These bacteria might contribute to degradation of xenobiotics and tree weakening during the nematode tree infection.  相似文献   

Effect of Incorporating White,Red or Black Quinoa Flours on Free and Bound Polyphenol Content,Antioxidant Activity and Colour of Bread     

Ballester-Sánchez  Jaime  Gil  Jose Vicente  Haros  Claudia Monika  Fernández-Espinar  María Teresa 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(2):185-191

Plant Foods for Human Nutrition - Interest in quinoa as a functional food ingredient is currently emerging. The flours from white, red and black quinoa seeds were analysed in terms of total...  相似文献   

Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex     

Naranjo V  Gortazar C  Vicente J  de la Fuente J 《Veterinary microbiology》2008,127(1-2):1-9

Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and closely related mycobacteria of the Mycobacterium tuberculosis complex. They have an extensive host range and may cause zoonotic TB. A major obstacle to bTB eradication in livestock is the implication of wildlife in the natural cycle of the pathogen. The identification of wildlife reservoir hosts is crucial for the implementation of effective control measures. The European wild boar (Sus scrofa) is frequently considered a spillover or dead end host rather than a true reservoir, and scientific evidence is conflicting outside Mediterranean Spain. The aim of this review is to update current scientific evidence of the wild boar as a TB reservoir and to underline those aspects that need further research. Evidences supporting that wild boar is a TB reservoir host include: (i) presence of common M. tuberculosis complex genotypes in wild boar, domestic and wild animals and humans, (ii) high prevalence of M. bovis among wild boar in estates fenced for decades in complete absence of contact with domestic livestock, and other wild ungulates (iii) TB lesions are frequently seen in thoracic lymph nodes and lungs, suggesting that respiratory infection and excretion may occur, and (iv) extensive tuberculous lesions in more than one anatomical region occur in a high proportion of juvenile wild boar that probably represents the main source of mycobacterial excretion. Hence, epidemiological, pathological and microbiological evidence strongly suggests that, at least in Spanish Mediterranean ecosystems, wild boar are able to maintain TB infection in the wild and are most probably able to transmit the disease to other species, acting as a true wildlife reservoir. These results expand the list of wildlife species that act as natural reservoirs of TB in different parts of the world and suggest the need to control the infection in wild boar populations for the complete eradication of the disease in Spain.  相似文献   

The effects of sex and age on phytohaemagglutinin skin-testing of deer   总被引：1，自引：0，他引：1  

Fernandez-de-Mera IG  Vicente J  Hofle U  Rodriguez O  Gaspar-Lopez E  Gortazar C 《New Zealand veterinary journal》2008,56(2):71-73

AIM: To determine if there are sex- or age-related differences in the increase in skinfold thickness in response to the mitogen phytohaemagglutinin (PHA) in red deer. METHODS: One dose of 250 mug PHA was injected intradermally in the right side of the neck, and phosphate buffered saline (PBS) was injected at a second site as a control, in 110 (51 males and 59 females) captive Iberian red deer (Cervus elaphus hispanicus), ranging in age from 21 months to > or =5 years. Skinfold thicknesses were measured immediately before and 72 h following injection. RESULTS: There was a significant effect of gender on the average increase in skinfold thickness; males had greater increases (8.8 (SEM 0.57) mm) than females (4.23 (SEM 0.39) mm) after correcting for other confounding variables. No age-related differences were evident, but differences between sexes were more marked with increasing age. CONCLUSIONS AND CLINICAL RELEVANCE: Effects of gender, probably due to differences in energetic and reproductive constraints in red deer, should be taken into account when interpreting skinfold-test data, both in ecology and in the control of tuberculosis (Tb). Males tend to have a thicker skin than females, so skinfold increase relative to the thickness of the skin, rather than skinfold increase per se, should be used as a more appropriate measure of skinfold increase. This may also have clinical relevance in the interpretation of tuberculin skin testing.  相似文献   

Bovine tuberculosis in a badger (Meles meles) in Spain     

Sobrino R  Martín-Hernando MP  Vicente J  Aurtenetxe O  Garrido JM  Gortázar C 《The Veterinary record》2008,163(5):159-160

  相似文献