Effect of binary combination of some plant-derived molluscicides with MGK-264 or piperonyl butoxide on the reproduction of the snail Lymnaea acuminata     

Singh P  Singh VK  Singh DK 《Pest management science》2005,61(2):204-208

The effects of sub-lethal treatments (20 and 60% of 24-h LC(50)) with plant-derived molluscicides Annona squamosa, acetogenins, Argemone mexicana seed and protopine, in combination (1 + 5) with MGK-264 (ENT 8184) or piperonyl butoxide on the reproduction of Lymnaea acuminata has been studied. The plant-derived molluscicides and their active molluscicidal components, protopine and acetogenins, in combination with ENT 8184 or piperonyl butoxide caused a significant reduction in the fecundity, hatchability and survival of young snails. Combination of A squamosa seed powder with piperonyl butoxide was very effective as it caused a complete arrest of snail fecundity within 24 h of treatment. Removal of the snails to fresh water after the 96-h treatments caused a significant recovery in the fecundity of L acuminata.  相似文献   

Development of two new cytoplasmic male-sterile lines in Brassica juncea through wide hybridization   总被引：3，自引：0，他引：3  

M. Malik  P. Vyas    N. S. Rangaswamy  K. R. Shivanna 《Plant Breeding》1999,118(1):75-78

Two new cytoplasmic male-sterile (CMS) lines have been developed in Brassica juncea using the bridgecross hybrids (Diplotaxis erucoides×Brassica campestris)×B. juncea and (Diplotaxis berthautii×B. campestris)×B. juncea. These were backcrossed ×ve times with pollen of B. juncea. The CMS line (D. erucoides) B. juncea segregated into tall and short true breeding types; both resembled the cultivar B. juncea in vegetative and floral morphology and in cytology, except for a greater number of secondary branches and smaller anthers with empty sterile pollen in the CMS line. Female fertility was as good in the CMS line as in the cultivar. The other CMS line (D. berthautii) B. juncea resembled the cultivar in vegetative morphology and cytology. Four true breeding floral types were isolated as follows: (1) smaller and indehiscent anthers containing empty, sterile pollen, (2) all six stamens petaloid, (3) one petaloid stamen and five stamens antherless, and (4) apetalous flowers with all six stamens antherless.  相似文献   

QTL Analysis of Late Blight Resistance in a Diploid Potato Family of Solanum spegazzinii × S. chacoense     

S. K. Chakrabarti  B. P. Singh  Garima Thakur  Jagesh K. Tiwari  S. K. Kaushik  Sanjeev Sharma  Vinay Bhardwaj 《Potato Research》2014,57(1):1-11

Several quantitative trait loci (QTL) for resistance to late blight have been reported in diploid potatoes. The diploid wild potato species Solanum chacoense possesses a high degree of horizontal resistance to late blight. In the present study, we report on QTL mapping for late blight resistance in a diploid mapping population of 126 F₁ of Solanum spegazzinii (susceptible) × S. chacoense (resistant). The area under the disease progress curve (AUDPC) values for late blight resistance using the “whole plant in vitro assay” and the “detached leaf assay” on the mapping population displayed quantitative variation. Out of 64 AFLP primer-pairs combinations and SSR markers, a total of 209 significant AFLP loci were placed onto the 12 linkage group of potato covering a total map length of 6548.1 cM. QTL analysis based on the AUDPC dataset of the “whole plant in vitro assay” using the interval-mapping option identified two QTL (LOD?>?2.5) located on linkage groups IX and X, which explained 14.70 and 3.40% variation, respectively. The present study revealed the presence of potential new genetic loci in the diploid potato family contributing to quantitative resistance against late blight.  相似文献   

Interspecific hybridization between rice bean (Vigna umbellata) and its wild relative (V. minima): Fertility-sterility relationships     

M. C. Gopinathan  C. R. Babu  K. R. Shivanna 《Euphytica》1986,35(3):1017-1022

Summary The pre-and post-fertilization barriers in the interspecific crosses between Vigna umbellata and V. minima were investigated. In the reciprocal crosses (V. minima as the parent) the entry of pollen tubes into the ovary was delayed by about 4 h, and no seed set was observed. However, no pre-fertilization barriers were encountered in crosses involving V. minima as the parent and V. umbellata as the parent (normal cross). The delay/absence of divisions in the endosperm and the failure of embryo to divide were the post-fertilization barriers responsible for somatoplastic sterility in normal crosses which yielded a few hybrid seeds. The hybrid seeds showed poor germinability. The F₁ hybrids were intermediate between the parents in most morphological characters, and are completely sterile for pollen. Backcrossing of F₁ hybrid with both the parents did not restore fertility in the progenies. V. minima is considered as the tertiary gene pool of the rice bean.  相似文献   

Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets   总被引：1，自引：0，他引：1  

Meghna Rana Malik  N.S. Rangaswamy  K.R. Shivanna 《Euphytica》2001,120(2):195-203

Anther culture of two wide hybrids (Diplotaxis erucoides × Brassica campestris) × B. juncea and (D. berthautii × B. campestris) × B. juncea, their CMS lines and the parent species elicited a range of responses highlighting the importance of the genotype. Androgenesis was expressed in cultured anthers of CMS (D. erucoides) B. juncea (22.8%), in restored pollen fertile plants of this CMS line (1.66%), and in the parent, B. juncea cv Pusa Bold (13.02%). AgNO₃ was essential for androgenic response in the CMS lines, and it markedly increased the frequency of androgenesis in the cultivated species. Multiple crops of microspore embryos were obtained from responsive anthers of CMS plants in anther recultures. As high as92% microspore embryos of the CMS line germinated on basal B₅medium and formed normal plantlets. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Recombinant origin of the retrovirus XMRV     

Paprotka T  Delviks-Frankenberry KA  Cingöz O  Martinez A  Kung HJ  Tepper CG  Hu WS  Fivash MJ  Coffin JM  Pathak VK 《Science (New York, N.Y.)》2011,333(6038):97-101

The retrovirus XMRV (xenotropic murine leukemia virus-related virus) has been detected in human prostate tumors and in blood samples from patients with chronic fatigue syndrome, but these findings have not been replicated. We hypothesized that an understanding of when and how XMRV first arose might help explain the discrepant results. We studied human prostate cancer cell lines CWR22Rv1 and CWR-R1, which produce XMRV virtually identical to the viruses recently found in patient samples, as well as their progenitor human prostate tumor xenograft (CWR22) that had been passaged in mice. We detected XMRV infection in the two cell lines and in the later passage xenografts, but not in the early passages. In particular, we found that the host mice contained two proviruses, PreXMRV-1 and PreXMRV-2, which share 99.92% identity with XMRV over >3.2-kilobase stretches of their genomes. We conclude that XMRV was not present in the original CWR22 tumor but was generated by recombination of two proviruses during tumor passaging in mice. The probability that an identical recombinant was generated independently is negligible (~10(-12)); our results suggest that the association of XMRV with human disease is due to contamination of human samples with virus originating from this recombination event.  相似文献   

Dravya,a product of seaweed extract (<Emphasis Type="Italic">Sargassum wightii</Emphasis>), induces resistance in cotton against<Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv.<Emphasis Type="Italic">malsvacearum</Emphasis>     

Vinay B. Raghavendra  S. Lokesh  H. S. Prakash 《Phytoparasitica》2007,35(5):442-449

Dravya, a commercially developed aqueous seaweed extract, was evaluated for its effect on the expression of symptoms of bacterial blight caused byXanthomonas campestris pv.malvacearum (E.F. Smith) Dye in cotton. Seed soaking with Dravya (1:500) followed by foliar spray thrice at intervals of 10 days (10, 20, 30 days after sowing) resulted in a reduction in blight incidence on plants by 66%, 70% and 74% as determined 40, 60 and 80 days, respectively, after sowing. Induction of systemic resistance was associated with increases in plant height, total number of bolls formed, boll weight, stem girth, chlorophyll content, total phenols and peroxidase activity, which intimates that Dravya could be used as an ecofriendly potential input in the integrated management of bacterial-blight of cotton.  相似文献   

Single-slice dynamic computed tomographic determination of glomerular filtration rate by use of Patlak plot analysis in anesthetized pigs     

Alexander K  Del Castillo JR  Ybarra N  Morin V  Gauvin D  Authier S  Vinay P  Troncy E 《American journal of veterinary research》2007,68(3):297-304

OBJECTIVE: To compare glomerular filtration rate (GFR) as estimated from Patlak plot analysis by use of single-slice computed tomography (CT) with that obtained from clearance of plasma inulin in pigs. ANIMALS: 8 healthy anesthetized juvenile pigs. PROCEDURES: All pigs underwent precontrast, whole-kidney, helical CT; postcontrast single-slice dynamic CT; and postcontrast, whole-kidney CT for volume determination. On dynamic images, corrected Hounsfield unit values were determined for each kidney and the aorta. A Patlak plot for each kidney was generated, and plasma clearance per unit volume was multiplied by renal volume to obtain whole-animal contrast clearance. Mean GFR determined via inulin clearance (Inu-GFR) was measured from each kidney and correlated to mean GFR determined via CT (CT-GFR) for the left kidney, right kidney, and both kidneys by use of linear regression and Bland-Altman analyses. RESULTS: CT-GFR results from 7 pigs were valid. Total and right kidney Inu-GFR were correlated with total and right kidney CT-GFR (total, R(2) = 0.85; right kidney, R(2) = 0.86). However, left kidney CT-GFR was poorly correlated with left kidney Inu-GFR (R(2) = 0.47). Bland-Altman analysis revealed no significant bias between Inu-GFR and CT-GFR for the left kidney, right kidney, or both kidneys. CONCLUSIONS AND CLINICAL RELEVANCE: CT-GFR as determined by use of a single-slice acquisition technique, low-dose of iohexol, and Patlak plot analysis correlated without bias with Inu-GFR for the right kidney and both kidneys (combined). This technique has promise as an accurate CT-GFR method that can be combined with renal morphologic evaluation.  相似文献   

Strategic Marketing Prospects for Developing Sustainable Medicinal and Aromatic Plants Businesses in the Indian Himalayan Region     

Pramod Chandra  Vinay Sharma 《Small-Scale Forestry》2018,17(4):423-441

Among the non-timber forest products, the medicinal and aromatic plants (MAP) are key forest resources for the world’s herbal medicine, cosmetics, health food and other natural products industries. MAP businesses are continuing to increase in many biodiversity-rich areas of the world. The overharvesting and depletion of forest based MAP and their unregulated trade are creating ecosystem and business sustainability issues for the MAP industry. This paper assesses the current state of the MAP industry in Uttarakhand State in the northwestern Himalayan region of India and offers marketing strategies for the conservation and sustainable commercialization of MAP in this region. A qualitative research method was first used involving interviews with policymakers and other industry stakeholders including local MAP traders and growers. A strengths, weaknesses, opportunities and threats analysis and a quantitative strategic planning matrix analysis were then conducted to identify appropriate marketing strategies. The study reveals that the existing practices of MAP businesses in Uttarakhand are inconsistent with the plants’ conservation requirements and the livelihoods of the primary producers. In its current form, the region’s MAP industry is therefore in a precarious position. However, if policymakers consider and act on the industry’s strengths and opportunities they could positively influence its sustainable development and help to minimize the negative impacts of the trade on the region’s forest MAP resources.  相似文献   

Genome-wide QTL analysis for pre-harvest sprouting tolerance in bread wheat     

Amita Mohan  Pawan Kulwal  Ravinder Singh  Vinay Kumar  Reyazul Rouf Mir  Jitendra Kumar  Manoj Prasad  H. S. Balyan  P. K. Gupta 《Euphytica》2009,168(3):319-329

A framework linkage map comprising 214 molecular marker (SSR, AFLP, SAMPL) loci was prepared using an intervarietal recombinant inbred line (RIL) mapping population of bread wheat. The RIL population that was developed from the cross SPR8198 (red-grained and PHS tolerant genotype) × HD2329 (white-grained and PHS susceptible genotype) following single seed descent segregated for pre-harvest sprouting (PHS). The RIL population and parental genotypes were evaluated in six different environments and the data on PHS were collected. Using the linkage map and PHS data, genome-wide single-locus and two-locus QTL analyses were conducted for PHS tolerance (PHST). Single-locus analysis following composite interval mapping (CIM) detected a total of seven QTL, located on specific arms of five different chromosome (1AS, 2AL, 2DL, 3AL and 3BL). These seven QTL included two major QTL one each on 2AL and 3AL. Two of these seven QTL were also detected following two-locus analysis, which resolved a total of four main-effect QTL (M-QTL), and 12 epistatic QTL (E-QTL), the latter involved in 7 QTL × QTL interactions. Interestingly, none of these M-QTL and E-QTL detected by two-locus analysis was involved in Q × E and Q × Q × E interactions, supporting the results of ANOVA, where genotype × environment interaction were non-significant. The QTL for PHS detected in the present study may be efficiently utilized for marker-aided selection for enhancing PHST in bread wheat.  相似文献