Diagnosis of highly pathogenic avian influenza in chickens: Bendigo 1992     

WM FORSYTH  DC GRIX  CA GIBSON 《Australian veterinary journal》1993,70(3):118-119

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Synchronization of Ovulation and Fixed Time Intrauterine Insemination in Ewes     

C Deligiannis  I Valasi  CA Rekkas  P Goulas  E Theodosiadou  T Lainas  GS Amiridis 《Reproduction in domestic animals》2005,40(1):6-10

A novel method for oestrus-ovulation synchronization in sheep followed by fixed time insemination is presented herewith. Mature dry ewes (n = 28) of Karagouniko breed being at an unknown stage of the oestrous cycle, were used during the middle of breeding season. The treatment protocol consisted of an initial administration of a GnRH analogue followed 5 days later by a prostaglandin F2alpha injection. Thirty-six hours later a second GnRH injection was administered to synchronize ovulation, and laparoscopic intrauterine insemination was performed 12-14 h later. Three days after insemination, fertile rams were introduced into the flock twice daily and oestrus-mating detection was carried out. For progesterone (P(4)) determination, blood samples were collected on alternate days, starting 2 days before the first GnRH injection and continuing for 17 days after insemination. An additional sample was taken on the day of insemination. Pregnancy diagnosis was carried out by trans-abdominal ultrasonography. Fourteen ewes (50%) conceived at insemination and maintained pregnancy; from the remainder 14 ewes 10 became pregnant at natural service, while four, although they mated at least two to three times, failed to conceive. In response to the first GnRH, P(4) concentration increased at higher levels in ewes that conceived at AI compared with those that failed to conceive (47.54 and 22.44%, respectively; p < 0.05). Significant differences (p < 0.05) in mean P(4) concentration between pregnant and non-pregnant animals were detected 1 day before AI (0.17 +/- 0.06 and 0.26 +/- 0.14 ng/ml, respectively) on the day of AI (0.15 +/- 0.04 and 0.24 +/- 0.08 ng/ml, respectively) as well as 9 and 11 days thereafter (0.48 +/- 0.12 and 0.38 +/- 0.12 ng/ml; 0.68 +/- 0.14 and 0.50 +/- 0.18 ng/ml, respectively). These results indicate that using the proposed protocol, an acceptable conception rate can be achieved which could be further improved by modifying the time intervals between interventions.  相似文献   

Ovum Pick-up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval     

C Rodríguez  L Anel  M Alvarez  E Anel  JC Boixo  CA Chamorro  P de  Paz 《Reproduction in domestic animals》2006,41(2):106-113

In vivo ovum pick-up (OPU) in sheep may be improved with a proper choice of aspiration elements (needle and tubing) and aspiration vacuum pressure. In the present study, two experiments were carried out. In Expt 1, visible follicles in ovaries of slaughtered ewes (treated separately according to their diameters: small<3 mm, medium 3-5 mm and large>5 mm) were aspirated using different combinations of the three studied factors such as aspiration flow rate (10, 20, 30, 40 and 50 ml water/min), needle gauge (18 and 20 G) and tubing inner diameter (1, 2 or 3 mm internal diameter). In Expt 2, a study with two 18 G needles of different lengths (18 G: 82 mm; 18 GL: 600 mm) was carried out, using ovaries obtained post-mortem, and performing in vivo laparoscopic follicular aspiration on ewes. We considered good quality oocytes as those with both complete compact cumulus and a homogeneous cytoplasm. Recovery rate, proportion of good quality oocytes (good quality oocytes/100 oocytes recovered) and overall efficiency (good quality oocytes/100 follicles aspirated) were noted. In Expt 1, aspiration flow rate affect remarkable proportion of good quality oocytes (69.5%, 50.5%, 44.8%, 36.5% and 28.3% for flows from 10 to 50 ml/min respectively, p<0.05). Needle gauge did not affect aspiration device efficiency. Thin and intermediate tubings were more effective (overall efficiency rates: 34.9%, 32.3% and 28.1% for 1, 2 and 3 mm respectively, p<0.05). Follicle size did not affect recovery rate, but proportion of good quality oocytes was higher for large (77.9%) and medium (64.4%) follicles (p<0.05). Finally, some combinations of the aspiration device showed greater effectiveness. In Expt 2, needle length did not influence recovery rate, but good quality oocytes rate was significantly modified both post-mortem and in vivo (good quality rate for 18 G vs 18 GL needles: 69.5% vs 47.7% and 58.1% vs 25.4%, post-mortem and in vivo respectively, p<0.05). We conclude that low-aspiration flow rates (10 and 20 ml/min) with thin or intermediate tubings (1 and 2 mm), and any short needle (18 G or 20 G) are the most adequate aspiration factors for OPU in sheep.  相似文献   

Relationships among Puberty,Muscle and Fat,and Liveweight Gain during Mating in Young Female Sheep          下载免费PDF全文

CA Rosales Nieto  MB Ferguson  H Thompson  JR Briegel  CA Macleay  GB Martin  AN Thompson 《Reproduction in domestic animals》2015,50(4):637-642

Greater depths of muscle are associated with better reproductive performance in ewe lambs, but, in adult ewes, reproductive performance also seems to vary with liveweight gain during the mating period. Therefore, in a large field study with Merino ewe lambs, we tested whether the relationships among eye muscle depth (EMD), fat depth (FAT) and reproductive performance depend on liveweight gain during the mating period. We selected lambs with a wide range in phenotypic values for depths of eye muscle (EMD) and fat (FAT) and assigned them to dietary treatments designed to achieve low (LOW, n = 244) or high (HIGH, n = 237) rates of liveweight gain during a 28‐day mating period. The LOW treatment maintained live weight, whereas the HIGH treatment gained 179 ± 3.8 g/day (p < 0.001). From those ewe lambs that attained puberty, first oestrus was detected at live weight 37.8 ± 0.2 kg and age 232 days. The proportion of ewes that attained puberty increased with EMD (p < 0.01). Ewes from the HIGH treatment were more fertile (pregnant ewes per 100 ewes exposed to rams) and had a higher reproductive rate (foetuses in utero per 100 ewes exposed to rams; p < 0.001) than those from the LOW treatment. Fertility and reproductive rate were positively correlated with weight gain during mating as well as live weight at the start of mating, FAT and EMD (p < 0.05 to <0.001). We conclude that faster growth, due to either extra nutrition during mating or higher phenotypic potential for fat and muscle, will increase reproductive performance in ewe lambs mated at 8 months of age.  相似文献   

Effects of manipulations to detect sliding hiatal hernia in dogs with brachycephalic airway obstructive syndrome          下载免费PDF全文

Olivier Broux DVM  Cécile Clercx DVM  PhD  Dipl. ECVIM‐CA  Anne‐Laure Etienne DVM  PhD  Valeria Busoni DVM  PhD  Dipl. ECVDI  Stéphanie Claeys DVM  PhD  Dipl. ECVS  Annick Hamaide DVM  PhD  Dipl. ECVS  Frédéric Billen DVM  PhD  Dipl. ECVIM‐CA 《Veterinary surgery : VS》2018,47(2):243-251

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Letter to the Editor          下载免费PDF全文

G. Segev DVM  DECVIM‐CA  C. Palm DVM  DACVIM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(5):1562-1563

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Low glyphosate rates do not affect Citrus limonia (L.) Osbeck seedlings     

Renan Gravena  Ricardo Victoria Filho  Pedro Luis CA Alves  Paulo Mazzafera  Adriana R Gravena 《Pest management science》2009,65(4):420-425

BACKGROUND: Glyphosate is used to control weeds in citrus orchards, and accidental spraying or wind drift onto the seedlings may cause growth arrest owing to metabolism disturbance. Two experiments were carried out to investigate the effect of non‐lethal rates (0, 180, 360 and 720 g AI ha^?1) of glyphosate on four‐month‐old ‘Cravo’ lime, Citrus limonia (L.) Osbeck, seedlings. Photosynthesis and the concentrations of shikimic acid, total free amino acids and phenolic acids were evaluated. RESULTS: Only transitory effects were observed in the contents of shikimate and total free amino acids. No visual effects were observed. CONCLUSION: The present study showed that glyphosate at non‐lethal rates, which is very usual when accidental spraying or wind drift occurs in citrus orchard, did not cause severe metabolic damage in ‘Cravo’ lime seedlings. Copyright © 2009 Society of Chemical Industry  相似文献   

Pressure-induced amorphization and negative thermal expansion in ZrW2O8     

CA Perottoni  JAH Jornada 《Science (New York, N.Y.)》1998,280(5365):886-889

It has recently been shown that zirconium tungstate (ZrW2O8) exhibits isotropic negative thermal expansion over its entire temperature range of stability. This rather unusual behavior makes this compound particularly suitable for testing model predictions of a connection between negative thermal expansion and pressure-induced amorphization. High-pressure x-ray diffraction and Raman scattering experiments showed that ZrW2O8 becomes progressively amorphous from 1.5 to 3.5 gigapascals. The amorphous phase was retained after pressure release, but the original crystalline phase returned after annealing at 923 kelvin. The results indicate a general trend between negative thermal expansion and pressure-induced amorphization in highly flexible framework structures.  相似文献   

Elevation change of the southern greenland ice sheet     

CH Davis  CA Kluever  BJ Haines 《Science (New York, N.Y.)》1998,279(5359):2086-2088

Seasat and Geosat satellite altimeter measurements for the Greenland ice sheet (south of 72 degreesN latitude) show that surface elevations above 2000 meters increased at an average rate of only 1. 5 +/- 0.5 centimeters per year from 1978 to 1988. In contrast, elevation changes varied regionally from -15 to +18 centimeters per year, seasonally by +/-15 centimeters, and interannually by +/-8 centimeters. The average growth rate is too small to determine if the Greenland ice sheet is undergoing a long-term change due to a warmer polar climate.  相似文献   

Dystocia and Fetotomy Associated with Cerebral Aplasia in a Greater One‐horned Rhinoceros (Rhinoceros unicornis)     

W Schaftenaar  T Fernandes  G Fritsch  R Frey  CA Szentiks  R‐D Wegner  TB Hildebrandt  R Hermes 《Reproduction in domestic animals》2011,46(1):e97-e101

The captive greater one‐horned rhinoceros population consists of 176 animals. Since 1971, a total of 226 calves were born into this captive population. However, 24% of the offspring born were either stillborn or did not survive the first 3 months. The causes for this high rate of stillbirth and neonate mortality have not yet been documented. Here, we report on the veterinary management of a dystocia and foetotomy resulting from a malpositioned greater one‐horned rhinoceros foetus. The dead foetus presented with a forelimb flexed at the shoulder joint, with all other joints extended. The foetus was dissected into five parts and extracted during two anaesthesias on two consecutive days. The dam recovered fully and came into oestrous 31 days after surgery. Post‐mortem and CT examination of the malformed foetal head revealed cranioschisis with cerebral aplasia and cerebellar hypoplasia. The cerebral aplasia presented here and in other recent cases suggests that neural tube defects and cranial malformations may be associated with more captive rhinoceros stillbirths than previously considered. Epidemiologic studies of these phenomena and possible nutritional deficiencies or hereditary defects are warranted.  相似文献