Detection of the K99 antigen of Escherichia coli in calf faeces by enzyme-linked immunosorbent assay (ELISA)     

Ellens DJ  de Leeuw PW  Rozemond H 《The Veterinary quarterly》1979,1(4):169-175

Summary An enzyme-linked immunosorbent assay (ELISA) is presented for the detection of the K99 antigen of Escherichia coli in calf faeces. False-positive reactions were not observed with K99-negative strains and with several viral antigens. Only bovine coronavirus caused slight positive reactions which could be eliminated by a blocking test. As compared with the conventional procedure for the detection of the K99 antigen, ELISA seemed to be at least as sensitive and had the advantage that samples could be stored at -20° C before testing. In addition many samples could be handled at the same time and the results became available quickly. By carrying out the assay as a blocking test, specific antibody against K99 in serum or colostrum could be detected and titrated.  相似文献   

Serum chloramphenicol levels and the intramuscular bioavailability of several parenteral formulations of chloramphenicol in ruminants     

Nouws JF  Ziv G 《The Veterinary quarterly》1979,1(1):47-58

Summary Serum chloramphenicol concentrations were determined by microbiological and chemical assay methods in cows, ewes, and goats treated parenterally with seven different veterinary parenteral chloramphenicol products, including the water soluble sodium succinate ester of chloramphenicol and solutions of 20%, 25% and 50% of chloramphenicol base in various organic solvents. Serum drug concentrations were analyzed for the effect of product formulation differences, dosage, whether the drug was administered i.m. at a single body site or to two sites, and the method of assay, on the absorption from the injection site, peak drug levels, and the persistence in serum of effective concentrations of the drug i.e. 5 to 10 ug / ml. Although differences were observed among the 6 products containing chloramphenicol base in respect to absorption rate and peak serum drug levels, and although these differences significantly influenced the persistence of microbiologically-active serum drug concentrations at the level of ≥ 10 μg / ml, they did not at the level of ≥ 5 μg / ml. In the animal species examined, injections given at 2 sites appeared to influence the duration of predetermined serum drug levels more than the differences among the products in respect of the absorption and elimination rates from serum, the peak serum concentrations, and the dose. The shapes of the concentration-to-time curves in cows and ewes injected with the same dose of a given product were essentially the same, but they were different in goats. Serum chloramphenicol concentrations measured chemically after treatment with chloramphenicol base were 20% to 46% higher than those measured microbiologically. For 60 minutes after the sodium succinate ester had been administered i.v. and i.m. to ewes, the chemically determined chloramphenicol levels were more than twice as high as the respective concentrations determined by microbiological assay, but thereafter, the magnitude of those differences was not greater than observed after treatment with chloramphenicol base. Intramuscular bioavailability of the products containing chloramphenicol base injected at 2 sites was rather poor (51% to 80.5%ofthe dose) and even lower values were calculated after injection at a single site. Results are briefly discussed of the effect of dosage form on the persistence of microbiologically effective serum drug levels. A dose of at least 50 mg / kg to be administered i.m. at two sites are essential prerequisits for chloramphenicol therapy in ruminants.  相似文献   

Blood parasites of sheep in the Netherlands. I. Anaplasma mesaeterum sp.n. (Rickettsiales, Anaplasmataceae)     

Uilenberg G  van Vorstenbosch CJ  Perié NM 《The Veterinary quarterly》1979,1(1):14-22

Summary On two occasions an anaplasm was isolated from sheep on the Dutch island of Ameland. The organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. Treatment with oxytetracycline was effective. Its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. This anaplasm differs from Anaplasma ovis in that less than 30% of the organisms are marginally situated in the red cell, as against over 70% in A. ovis; cross-immunity with A. ovis was incomplete and the latter appeared to be far more pathogenic to goats than the Dutch anaplasm, for which the name Anaplasma mesaeterum sp.n. is proposed. Its ultrastructure is similar to that of A. marginale and A. ovis. The vector is either Ixodes ricinus or Haemaphysalis punctata. Its practical importance remains to be ascertained.  相似文献   

Prevalence of antibodies to equine viruses in the Netherlands     

de Boer GF  Osterhaus AD  van Oirschot JT  Wemmenhove R 《The Veterinary quarterly》1979,1(2):65-74

Summary The prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the Netherlands between 1963 and 1966 and from 1972 onwards. Neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. The observed incidence of serum samples positive to equine adenovirus in the complement fixation test was 39%. Precipitating antibodies to equine infectious anaemia virus were detected only in serum samples from two horses imported from abroad. Haemagglutination inhibiting antibodies to Myxovirus influenzae A / equi-1, M. Influenzae A / equi-2, and Reovirus types 1, 2, and 3 were present in respectively 82%, 50%, 10%, 33% and 3.6% of the serum samples tested. The most frequently observed incidence of antibodies to the various equine respiratory viruses occurred in the groups of horses having repeatedly contact with other horses.  相似文献   

Patent ductus arteriosus.     

W D Liska  L P Tilley 《Veterinary Clinics of North America: Small Animal Practice》1979,9(2):195-206

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小麦丛矮病研究之三:灰飞虱同发病的关系     

蔡祝南  魏德忠  林志亮  杨莉  梅汝鸿  裘维蕃 《植物保护学报》1979,6(2):35-46

1976—1977年河北晋县贺家寨大队小麦春季灰飞虱的传带病毒率为31—52%,不同地块同代灰飞虱的带毒率异差较大:而秋苗期则为12—35.4%,其中不同地块上同代(四代成虫)灰飞虱的带毒率的差异从33.3%至35.4%则不太大。灰飞虱的有效传毒指数(即虫口数×自然带毒率)同田间小麦发病率的相关性是非常显著的,其r=0.9769。1975—1976两年调查及计算分析结果,其直线回归公式如y=4.12x-2.577。在有效传毒指数0.7—20.9之间用于予测发病率有效。人工分期侵染试验证明小麦在生长发育过程中受侵愈早,病情愈重,其中死株率也愈大。秋季侵染的死株率达95.6%,病指为99.6;夏季侵染的死株率为0,病指为57.6。秋季小麦出苗前后的大量虫口形成小麦10月中下旬的一个发病高峰,第二年春季4月中旬的虫口高峰形成5月上旬的一个发病高峰。3月下旬的一个发病高峰是越冬前受侵的。病毒在小麦植株中的潜育期的长短随小麦的生长速度而异。温度不过其中因素之一,最短5—8天,最长30—40天。侵染后如小麦进入越冬期,即不表现症状,一直要到返青以后。介体灰飞虱在河北晋县以第五代3—4龄若虫在冬麦地及草荒中越冬。夏季在玉米上可以产卵并孵化,但不能长期存活,主要在地头沟边及夏作物荫蔽下的禾草上越夏。冬麦治虫防病的重点应在秋季麦苗出土前,春季则应在3月中旬左右。冬麦适当推迟播种有利于减少发病率。棉间作麦比平作的病情重六倍而粮麦间作的病情比平作的重九倍。  相似文献   

小麦对条锈病的水平抗病性研究初报   总被引：4，自引：0，他引：4  

曾士迈  王沛有  武修英  张万义  王吉庆  宋位中  王生元 《植物保护学报》1979,6(1):1-10

将小麦品种采用随机区组的田间设计种于小种病圃中,以鉴定和测定其水平抗性。根据各个“品种——小种”组合的相对病情指数的方差分析的结果,有些品种,如农大311、西农6028和丰产3号,其品种——小种互作高度显著,被鉴定为具有垂直抗性,另外一些品种,对试验所用的小种并无专化性,并且在大田生产中已显出20多年的持久的中度抗性,初步推测为属水平抗性。在后一类中,有些品种,如平原50,表现为中等反应型,其余则属于呈典型感病的迟锈慢锈类型。在迟锈慢锈品种的病指的对数矫正值(logits)和病圃的锈病强度(以感病对照品种的病指表之)的对数矫正值之间发现了直线回归。  相似文献   

Tumours of Begonia and some other ornamentals,induced by Corynebacterium fascians     

H. A. Van Hoof  H. Huttinga  A. Knaap  H. P. Maas Geesteranus  W. H. M. Mosch  D. G. J. De Raay-Wieringa 《European journal of plant pathology / European Foundation for Plant Pathology》1979,85(3):87-98

In 1975 many tumours were observed in plants ofBegonia Schwabenland grown in Aalsmeer. Submersion of the roots ofNicotiana megalosiphon seedlings in a homogenate of tumorous tissue, induced tumours after two weeks. Short periods of submergence yielded results similar to those obtained after longer periods. Tumour homogenates lost their infectivity after ten min at 50°C. Aphids transmitted the infectious agent.Treatment with propylene oxide did not inhibit infectivity completely. Filtration through a 450 nm filter removed the infectious agent.Tobacco tumor virus or a viroid could not be isolated. Cultures ofCorynebacterium fascians, isolated from tumours ofN. megalosiphon were highly infectious and induced tumours in healthyN. megalosiphon andBegonia. Tumorous tissue homogenates ofPelargonium zonale, Dahlia sp.,Gladiolus sp., andLilium sp. also caused tumours inN. megalosiphon, from whichC. fascians was isolated. It was not possible to produce tumours inN. megalosiphon with homogenates from roses with symptoms of bud proliferation.Samenvatting In 1975 werden vele tumoren waargenomen inBegonia Schwabenland op Aalsmeerse bedrijven (Fig. 1). De infectiositeit van tumorweefsel kon goed en snel worden vastgesteld door de wortels van zaailingen vanNicotiana megalosiphon in een homogenaat van tumorweefsel te dompelen. Tumoren ontstonden na twee weken, de eindbeoordeling geschiedde na een maand (Fig. 2). Ook verschillende andereNicotiana spp.,Melilotus officinalis (Fig. 3) enPisum odoratum (Fig. 4) werden aangetast.Bij de infectiositeitstoets gaven zeer korte dompeltijden even goede resultaten als langere (Tabel 1). Infectieus sap verloor zijn infectievermogen na 10 min verhitting bij 50°C. Bladluizen brachten de smetstof over. Propyleenoxide verminderde de infectiositeit wel, doch onderdrukte deze niet totaal. Bij filtratie door een 450 nm filter bleef het infectieuse agens op het filter achter. Het tumor-inducerende agens was ook aanwezig in die delen van planten met tumoren welke gezond leken en het ging voor een gering deel over met zaad (Tabel 2).Uit tumoren konden wij geen tabakstumorvirus of een viroïde isoleren. Culturen vanCorynebacterium fascians, geïsoleerd uit tumoren vanN. megalosiphon bleken zeer infectieus en veroorzaakten tumoren inN. megalosiphon enBegonia. Homogenaten van tumorweefsel vanPelargonium zonale, dahlia (Fig. 5), gladiool (Fig. 6) enLilium Mid Century Hybrid Enchantment (Fig. 7) veroorzaakten ook tumoren opN. megalosiphon, waaruitC. fascians werd geïsoleerd. Met sap van kroeskopzieke rozen konden wijN. megalosiphon niet besmetten.  相似文献   

DDT in northern pike (Esox lucius) from the Richelieu River, Québec, Canada, 1974-75     

S Boileau  M Baril  J G Alary 《Pesticides monitoring journal》1979,13(3):109-114

Concentrations of DDT, TDE, DDE, and sigma DDT were determined in homogenized whole fish samples of 129 northern pike (Esox lucius). These fish were netted between June 1974 and June 1975 in the first 10 km of the Richelieu River flowing in Canadian territory. Two years after the banning of DDT, sigma DDT levels ranged from 0.2 ppm fresh body weight in two-year-old specimens to 1.5 ppm in a six-year-old pike. Residues increased greatly with age, and significant seasonal variations in the sigma DDT levels were found in five- and six-year-old pike.  相似文献   

苹毒蛾核多角体病毒观察初报     

齐人礼  王立臣  刘惠敏  毕庶春  张兴达 《植物保护学报》1979,6(3):33-36

苹毒蛾病毒是典型的核型多角体病毒结构,对苹毒蛾幼虫有较强的致死能力,对于开展苹毒蛾的生物防治有一定意义;在病毒分类上根据形态鉴别,可能属于杆状病毒属(Baculovirus)亚组(A)的一个种。  相似文献