Autologous Whole Ram Seminal Plasma and its Vesicle-free Fraction Improve Motility Characteristics and Membrane Status but not In Vivo Fertility of Frozen–Thawed Ram Spermatozoa     

R El-Hajj Ghaoui  PC Thomson  T Leahy  G Evans  WMC Maxwell 《Reproduction in domestic animals》2007,42(5):541-549

Motility characteristics (assessed subjectively and with computer-assisted semen analysis) and membrane status (after staining with chlortetracycline) of washed and non-washed frozen-thawed ram spermatozoa were evaluated after incubation in buffer and buffer containing autologous whole seminal plasma or one of its two fractions: the pellet of membrane vesicles obtained by ultracentrifugation (and used at three times normal protein concentration) or the vesicle-free supernatant fraction. Whole seminal plasma and supernatant, but not membrane vesicles, improved the motility characteristics of spermatozoa after 3 and 6 h of post-thaw incubation compared with the control buffer. Resuspension and incubation with whole seminal plasma, supernatant or membrane vesicles lowered the proportion of acrosome-reacted frozen-thawed spermatozoa compared with the control buffer. Unwashed frozen-thawed semen from three rams, incubated with autologous whole seminal plasma or its fractions and inseminated using cervical or intrauterine artificial insemination, had no effect on pregnancy rates of ewes in synchronized oestrus. However, fertility was higher after laparoscopic than cervical insemination (44.9 vs 12.3%, p < 0.001). In conclusion, resuspension and incubation of frozen-thawed ram spermatozoa in autologous whole seminal plasma or its vesicle-free supernatant fraction improved their motility characteristics and, with membrane vesicles, membrane status, but these benefits were not reflected in improved fertility after cervical or intrauterine insemination.  相似文献   

Integration of forestry and agriculture—A model     

T.J. Maxwell  A.R. Sibbald  J. Eadie 《Agricultural Systems》1979,4(3):161-188

This paper describes a modelling approach to the allocation of land as between forestry and agriculture in the context of hill and upland. The model can be used to examine objectively the outcome of some of the stated objectives and constraints concerned with integration. Examples in the use of the model are presented.  相似文献   

Effect of protein level on growth, nitrogen balance and reproductive performance in gilts     

R D Jones  C V Maxwell 《Journal of animal science》1974,39(6):1067-1072

  相似文献   

Effect of dietary particle size on lesion development and on the contents of various regions of the swine stomach   总被引：1，自引：0，他引：1  

C V Maxwell  E M Reimann  W G Hoekstra  T Kowalczyk  N J Benevenga  R H Grummer 《Journal of animal science》1970,30(6):911-922

  相似文献   

Use of tritiated water to assess, in vivo, the effect of dietary particle size on the mixing of stomach contents of swine     

C V Maxwell  E M Reimann  W G Hoekstra  T Kowalczyk  N J Benevenga  R H Grummer 《Journal of animal science》1972,34(2):212-216

  相似文献   

Eosinophilia associated with facial oedema in fowls     

M H Maxwell  W G Siller  G M Mackenzie 《The Veterinary record》1979,105(10):232-233

  相似文献   

Equine seminal plasma derived lactoferrin regulates binding of polymorphonuclear neutrophils (PMNs) to spermatozoa     

M.H.T. TroedssonA. Esteller-Vico  K.E. ScogginE.M. Woodward  E.L. SquiresB.A. Ball  H. Maxwell 《Journal of Equine Veterinary Science》2014

  相似文献   

Aging. Antiaging research and the need for public dialogue     

Juengst ET  Binstock RH  Mehlman MJ  Post SG 《Science (New York, N.Y.)》2003,299(5611):1323

  相似文献   

Phytopathology     

Maxwell DP 《Science (New York, N.Y.)》1978,199(4326):289-290

  相似文献   

Negative effects of energy supplementation at peak lactation of sheep can be offset by the addition of Lactobacillus-fermented plant extracts     

Matthew R Beck  Konagh Garrett  Kenneth J Olejar  Thomas M R Maxwell  Craig R Bunt  Andrew E Greer  Pablo Gregorini 《Journal of animal science》2021,99(5)

Energy supplementation may reduce oxidative stress by correcting a negative energy balance, but in some contexts, it has been shown to increase oxidative stress, especially at peak lactation. The current experiment examined if a pelleted energy supplement with or without the addition of Lactobacillus-fermented seaweed or seaweed plus terrestrial plants extracts affected oxidative stress of ewes from late gestation through to weaning and ewe and lamb production from lambing to weaning. Treatments were either no supplement (CON−), a pelleted supplement only (CON+, 100 g/ewe per d), CON+ with seaweed extract only (SWO, 10 mL/ewe per day), or CON+ with seaweed plus an arrangement of terrestrial plant extract (SWP, 10 mL/ewe per d). Ewes (n = 160; mean initial BW = 72.3 ± 9.5 kg [mean ± SD]) were randomized to pastures (n = 4 pastures per treatment with 10 ewes each). After lambing, ewes with twins were reallocated to pastures (n = 3 pastures per treatment with 10 ewes each) according to lambing date. At 4 wk in milk, supplementation tended to reduce total antioxidant status (TAS; P = 0.10) and increased glutathione peroxidase (GPx) activity compared with nonsupplemented ewes (P = 0.04). The addition of seaweed and terrestrial plants extracts to the concentrate, that is, SWO and SWP, increased TAS and reduced GPx activity compared with CON+ (P < 0.01). Supplementation increased milk yield at weeks 4, 6, and 8 of lactation, and protein, lactose, and total milk solids yield at peak lactation (week 4; P < 0.05). The CON− ewes had greater somatic cell count than the supplemented ewes at weeks 4, 8, and 10 of lactation (P = 0.03). Our results suggest that energy supplementation, alone, increases oxidative stress of lactating ewes, which may relate to increased oxidative phosphorylation. Most importantly, these results indicate that in situations where energy supplementation is needed to increase animal performance, negative effects of energy supplementation around peak lactation can be offset by the addition of Lactobacillus-fermented plant extracts (SWO and SWP) to improve antioxidant status.  相似文献