Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment     

HUANG Feng  WAN Yong-ling  WU Da-long  LV Huan-zhang  GUO Jun-hua 《园艺学报》2003,19(8):1045-1048

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.  相似文献   

Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro     

XIE Tong  WU Ping  TANG De-shen  JIANG Li-ming 《园艺学报》2003,19(3):391-393

AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.  相似文献   

Effects of the juice of Fructus Hippophae (JFH) on thrombocytopenia induced by cyclophosphamide in rats     

GE Zhi-hong  L IANG Yi  CHEN Yun-xian  WU Yao-heng  ZHOU Hong  LI Da 《园艺学报》2003,19(5):693-695

AIM:To investigate the effects of the juice of fructus hippophae(JFH) on thrombocytopenia in rats.METHODS:Forty-eight wistar rats were divided into control group, model group, Yixuesheng-Jiaonang (YSJN) group and JFH group. Rats were injected intraperitoneally with cyclophosphamide (CTX, 30 mg/kg) or saline once a day for 3 days. And then, Saline, YSJN or JFH was administered (ig) once a day for 11 days. On the second, fouth, sixth and eighth day, the thrombocyte were counted, the mean platelet volume (MPV) and PDW were examined. On the eighth day, the cAMP, cGMP in platelets and platelet aggregation function were also examined.RESULTS:JFH could shorten the time of blood coagulation, increase the count of platelet, and enhance the platelet aggregation function. The experiment also showed that the JFH could decrease cGMP content in platelet.CONCLUSION:JFH could enhance blood coagulation and quality of platelet and prevent thrombocytopenia induced by cyclophosphamide in rats.  相似文献   

Effects of Sini decoction on atherosclerosis and ceramide content of aorta in rabbits     

HEI Zi-qing  WU Wei-kang  SUN Hui-lan  HUANG He-qing  TAN Hong-mei  LUO Han-chuan 《园艺学报》2003,19(3):345-347

AIM:To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS:28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg^-1·d^-1). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS:Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION:Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta.  相似文献   

Expression of peroxisome proliferator-activated receptor α in rat fatty liver     

QI Xiao-hong  ZHANG Zhao-ping  LI Xiao-yu  QUELing-li  WU Cui-zhen 《园艺学报》2003,19(9):1206-1209

AIM:To investigate the role of expression of peroxisome proliferator-activated receptor α(PPAR α) in pathogenesis of rat fatty liver.METHODS:The rats were treated with a low dose of carbon terachloride (CCl₄) and fed a high fat diet to produce fatty liver. We determined the concentrations of triglyceride (TG), total cholesterol (TC), free fatty acid (FFA) in liver and the alanine aminotransferase (ALT) activity, tumor necrosis factor-α (TNF-α), FFA in serum and the degree of hepatocytic steatosis. Total RNA of liver was extracted, and the expression of PPAR α were analyzed by semi-quantitative RT-PCR method.RESULTS:In model group, the hepatocytic PPAR α mRNA expression decreased to 0.41±0.28, compared to 1.41±0.29 in the control group (P＜0.01). The contents of TG, TC, FFA in model rat liver were (1.88±0.20) mmol·L^-1, (11.03±1.12) mmol·L^-1 and (1 260.38±151.27) μmol·L^-1, respectively, compared to (0.53±0.10) mmol·L^-1, (1.25±0.25) mmol·L^-1 and (334.30±27.09) μmol·L^-1 in the control group (P＜0.01). The activity of ALT, concentrations of TNF-α and FFA in serum were also increased remarkably in model group.CONCLUSION:Oxidation of fatty acid and utilization of lipids in liver are affected by reducing the expression of PPAR α, which result lipid accumulation in liver.  相似文献   

The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps     

CHEN Rong  SHI Jian-bo  XU Geng  DI Jing-fang  ZENG Shan  ZENG Yao-ying 《园艺学报》2003,19(12):1623-1626

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.  相似文献   

Effects of TGF-β₁ and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells(HSPC)     

HUANG Mei-juan  CHEN Yuan-zhong  WU Yong  LI Nai-nong  ZHANG Zhao-xiu 《园艺学报》2003,19(10):1375-1378

AIM:To study the effect of TGF-β₁ and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD₃₄⁺cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β₁ and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD₃₄⁺ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β₁ antisense PS-ODNS cooperated with cytokines increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β₁ and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo.  相似文献   

黑河流域荒漠地区梭梭人工林地土壤水分动态研究   总被引：29，自引：7，他引：29  

刘发民  张应华  仵彦卿  张小军 《干旱区研究》2002,19(1):27-31

梭梭林地土壤剖面的含水量与裸沙地含水量显著不同 ,裸沙地土壤含水量随深度增加而增加 ,林地在 30～ 12 0cm范围内有较高的含水量 ;林地 0～ 30cm的上层土壤含水量受降雨及蒸发的剧烈影响 ,30～ 2 0 0cm范围内的林地土壤各测量层平均含水量与其标准差有二次函数的关系 ;降雨对每月的 0～ 2 0 0cm的土壤贮水量没有明显的影响 ,说明降雨多数在当月就被蒸散和深层渗漏所消耗 ;认为梭梭人工林这种脆弱生态系统能够在严酷的环境条件下存在 ,其根系对土壤水和地下水有吸水和释水的再分配功能是重要的因素之一  相似文献   

乡村生态学研究的尺度与等级特征   总被引：2，自引：0，他引：2  

何念鹏  周道玮  吴泠 《干旱区资源与环境》2002,16(2):22-26

乡村生态学 ( Village ecology)的尺度和等级特征依赖一定空间区域 ,不同尺度所研究村落的特征也不尽相同 ,空间尺度的大小很大程度上决定于所研究村落的复杂性、稳定性及特征。在特定的空间尺度下 ,乡村的组成要素随着时间尺度的变化而变化 ,但变化速率受空间尺度大小的制约。乡村生态学研究具有等级结构 ,空间尺度决定所研究单元的等级位置。行政村 (中心村 )和自然村 (自然屯 )是乡村生态学研究的基本尺度  相似文献   

麦蚜对吡虫啉的敏感性及吡虫啉有效用量的评价   总被引：1，自引：0，他引：1  

刘爱芝  王晓军  李素娟  武予清  李巧丝 《植物保护》2002,28(3):15-18

室内测定结果表明 ,禾谷缢管蚜对吡虫啉的敏感性比麦长管蚜高 ,其LC₅₀之比为1∶3左右。田间试验结果明确 ,吡虫啉持效性好 ,其持效期与吡虫啉用量和麦蚜种类有关。小麦穗蚜发生始盛期是吡虫啉防治麦蚜的最佳时期 ,防治禾谷缢管蚜最佳有效用药量为1g/667m²,麦长管蚜为2g/667m²。  相似文献