Morphometry,Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)          下载免费PDF全文

HVR Silva  FF Magalhães  LR Ribeiro  ALP Souza  CIA Freitas  MF de Oliveira  AR Silva  LDM Silva 《Reproduction in domestic animals》2015,50(6):945-951

The ring‐tailed coati (Nasua nasua) is a procyonid whose population is in sharp decline. Therefore, studies are needed to better understand the reproduction of this animal. For this reason, this study aimed to evaluate the morphology, morphometry and sperm ultrastructure of ring‐tailed coati sperm. Four captive adult males were used for this study. Slides stained with Bengal Rose were used for the morphometric and morphologic analyses. The length and width of the head were measured, as well as the length of the midpiece and tail and the total length of the sperm. Scanning electron microscopy and transmission electron microscopy were used for the ultrastructural analyses. The most obvious morphological abnormalities observed were coiled tails (6.1 ± 8.7%) and the lack of acrosomes (5.4 ± 4.4%). Regarding the morphometry, the measurements of the head (length × width), midpiece (length) and tail (length) were (mean ± SD) 6.2 ± 0.4 × 8.1 ± 0.6 μm, 14.1 ± 0.5 and 63.9 ± 4.1 μm, respectively, and the total length of the sperm was 86.1 ± 4.3 μm. Through electron microscopy, the presence of electron‐lucent points in the nucleus and the presence of approximately 55 mitochondrial spirals in the midpiece were identified. The data obtained in this study provide detailed information on the sperm characteristics of coatis and may inform future research on germplasm conservation, both for this species and other threatened procyonids.  相似文献   

Investigation of the allergenic potential of wines fined with various proteinogenic fining agents by ELISA     

Weber P  Steinhart H  Paschke A 《Journal of agricultural and food chemistry》2007,55(8):3127-3133

Hidden allergens are a common problem in food safety that has been known for many years. This is why the European Parliament adopted Directive 2003/89/EC amending 2000/13/EC. In addition to specific ingredients, Directive 2003/89/EC also requests the declaration of specific products that were used in the production and could be a risk for allergic individuals. This also includes the declaration of fining agents and lysozyme used in wines. In fact, it could be assumed that fining agents would be almost completely removed during the manufacturing process; however, until now there has been no necessity to analyze wine for these fining agents. By applying enzyme-linked immunosorbent assay (ELISA), residuals of fining agent proteins and the stabilizer lysozyme were investigated in various German wines. The results showed no detectable amounts of fining agents in wines, except for dried egg white and lysozyme, both derived from hen's egg white. For those products, adverse reactions against treated wines could not be excluded.  相似文献   

Trade-offs and synergies in management of two co-occurring specialist squash pests     

Brzozowski  Lauren J.  Weber  Donald C.  Wallingford  Anna K.  Mazourek  Michael  Agrawal  Anurag A. 《Journal of pest science》2022,95(1):327-338

Journal of Pest Science - Co-occurring herbivorous pests may have shared or divergent responses to plant- and insect- derived cues, creating challenges for effective pest management in...  相似文献   

Carbon dynamics in small tropical catchments under preserved forest and cacao agroforestry systems     

da Costa  Eline Nayara Dantas  de Souza  Jéssica Carneiro  Pereira  Marilane Andrade  de Souza  Marcelo F. Landim  de Souza  Weber F. Landim  da Silva  Daniela Mariano Lopes 《Agroforestry Systems》2021,95(8):1647-1659

Agroforestry Systems - Inland waters such as streams that receive carbon from terrestrial landscapes usually have a net heterotrophic metabolism and emit significant amounts of CO2 to the...  相似文献   

Effect of Dietary Nonstructural Carbohydrate Content on Activation of 5′‐Adenosine Monophosphate‐Activated Protein Kinase in Liver,Skeletal Muscle,and Digital Laminae of Lean and Obese Ponies     

T.A. Burns  M.R. Watts  P.S. Weber  L.J. McCutcheon  R.J. Geor  J.K. Belknap 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(4):1280-1288

Background

In EMS‐associated laminitis, laminar failure may occur in response to energy failure related to insulin resistance (IR) or to the effect of hyperinsulinemia on laminar tissue. 5′‐Adenosine‐monophosphate‐activated protein kinase (AMPK) is a marker of tissue energy deprivation, which may occur in IR.

Hypothesis/Objectives

To characterize tissue AMPK regulation in ponies subjected to a dietary carbohydrate (CHO) challenge.

Animals

Twenty‐two mixed‐breed ponies.

Methods

Immunohistochemistry and immunoblotting for total AMPK and phospho(P)‐AMPK and RT‐qPCR for AMPK‐responsive genes were performed on laminar, liver, and skeletal muscle samples collected after a 7‐day feeding protocol in which ponies stratified on body condition score (BCS; obese or lean) were fed either a low‐CHO diet (ESC + starch, approximately 7% DM; n = 5 obese, 5 lean) or a high‐CHO diet (ESC + starch, approximately 42% DM; n = 6 obese, 6 lean).

Results

5′‐Adenosine‐monophosphate‐activated protein kinase was immunolocalized to laminar keratinocytes, dermal constituents, and hepatocytes. A high‐CHO diet resulted in significantly decreased laminar [P‐AMPK] in lean ponies (P = .03), but no changes in skeletal muscle (lean, P = .33; obese, P = .43) or liver (lean, P = .84; obese, P = .13) [P‐AMPK]. An inverse correlation existed between [blood glucose] and laminar [P‐AMPK] in obese ponies on a high‐CHO diet.

Conclusions and Clinical Importance

Laminar tissue exhibited a normal response to a high‐CHO diet (decreased [P‐AMPK]), whereas this response was not observed in liver and skeletal muscle in both lean (skeletal muscle, P = .33; liver, P = .84) and obese (skeletal muscle, P = .43; liver, P = .13) ponies.  相似文献   

Blood oxygen transport and hemoglobin function in three tropical fish species from northern Australian freshwater billabongs     

R.M.G. Wells  J. Baldwin  R.S. Seymour  R.E. Weber 《Fish physiology and biochemistry》1997,16(3):247-258

The oxygen binding properties of whole blood and hemoglobin were studied in three phylogenetically distant tropical teleosts inhabiting freshwater billabongs that exhibit marked seasonal oxygen stratification. The water breathing saratoga (Scleropages jardinii, Family Osteoglossidae), derived from an ancient divergence of the stem line leading to all other extant teleosts, has a low blood oxygen carrying capacity, Bohr and Root effect, and a high blood oxygen affinity. Erythrocytes contain a single major hemoglobin component, and equimolar concentrations of ATP and GTP. The tarpon (Megalops cyprinoides, Family Megalopidae), a facultative air-breather, has the highest blood oxygen carrying capacity and Bohr effect, and a low blood oxygen affinity and Root effect. Erythrocytes contain a single major hemoglobin and ATP as a putative regulator of hemoglobin oxygen affinity. Barramundi (Lates calcarifer, Family Centropomidae), is an obligate water-breather with intermediate blood oxygen binding properties, and the smallest Root effect. Erythrocytes contain at least 7 hemoglobins, and equimolar concentrations of ATP and GTP. Functional properties of these three blood oxygen transport systems are considered in terms of the respiratory environment and demand for oxygen. Our interpretation supports the hypothesis that the process of speciation can lead to divergence in physiological mechanisms, irrespective of past or present selection pressures.  相似文献   

Hemoglobin and subunit multiplicity in the rainbow trout (Oncorhynchus mykiss) hemoglobin system     

A. Fago  E. Forest  R.E. Weber 《Fish physiology and biochemistry》2001,24(4):335-342

Hemoglobin (Hb) multiplicity is a commonly used index of phylogenetic differentiation and molecular adaptation in fish. Despite improvements in technologies for characterizing protein structure, our knowledge of the component composition of the Hb system in rainbow trout, Oncorhychus mykiss, has remained almost unchanged through three decades. The Hb is considered to consist of four components, of which two (HbI and HbIV) have been extensively characterised with regard to structure and function. Using a variety of molecular techniques we demonstrate the presence of at least nine different Hb fractions composed of nine (five α and four β globin) chains, and that HbI and HbIV consist of two and five individual components, respectively. These findings indicate that the published data on trout HbI and HbIV refer to mixtures of isoHbs and need to be reappraised. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Isolation of Campylobacter jejuni and Campylobacter coli in fecal samples of healthy slaughter swine depending on the season     

A Weber  C Lembke  R Sch?fer 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1985,32(1):40-45

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The effect of footrot on body weight and wool growth of sheep   总被引：1，自引：0，他引：1  

DJ MARSHALL  RI WALKER  BR CULLIS  MF LUFF 《Australian veterinary journal》1991,68(2):45-49

Body weight and traits associated with production of wool were measured over a 2-year period between 1985 and 1987 in south-western New South Wales in a flock of Merino wethers experimentally infected with footrot. The disease was allowed to spread freely amongst 150 of the flock but kept at very low prevalence in the remaining 50 by preventive footbathing during transmission periods. Severe, underrunning footrot had a significant adverse effect on body weight, for each year of the trial. Body weight was most severely reduced at times of the year when footrot was spreading among animals and lesions were severe. The mean body weight of the infected group at the end of the 2 years of observation was 7.3 kg (11.6%) below that of the control group. Footrot also depressed wool growth, with the mean clean fleece weight of the infected group being 0.4 kg (8%) lighter than that of the controls at each of the 2 annual shearings. There were no consistent differences between the groups for the other wool characteristics measured.  相似文献   

The occurrence of Cryptococcus neoformans in fecal samples from birds kept in human living areas]     

A Weber  R Sch?fer 《Berliner und Münchener tier?rztliche Wochenschrift》1991,104(12):419-421

With help of Guizotia creatinine agar (syn. bird seed agar) Cryptococcus (Cr.) neoformans var. neoformans was isolated from 71 (7.7%) of 925 investigated droppings of birds kept within human living area. Cr. neoformans was detected in droppings of 1.2% from 164 psittacines, in droppings of 1.7% from 118 budgerigars and in droppings of 18.4% from (carrier) pigeons. This yeast was not isolated from droppings of 13 small parrots, 10 eclectus parrots, 13 rosellas, 21 cockatiels, 6 Polytelidus sp., 32 Cacatua sp., 13 Carduelis sp., 5 waxbills, 5 starlings, 120 chicken, 9 turkeys, 2 geese, 2 Turdus sp., 1 duck and 1 gull.  相似文献