Comparison of the Johne''s absorbed EIA and the complement-fixation test for the diagnosis of Johne''s disease in cattle   总被引：2，自引：0，他引：2  

SE RIDGE  IR MORGAN  DC SOCKETT†  MT COLLINS†  RJ CONDRON  NW SKILBECK†  JJ WEBBER§ 《Australian veterinary journal》1991,68(8):253-257

A commercially available absorbed ELISA for the diagnosis of Johne's disease (JD) (paratuberculosis) in cattle, the Johne's Absorbed EIA, was compared with the conventional complement-fixation test (CFT) used in Australia. Stored plasma from 3 Victorian dairy herds with a history of JD, sera from specimens submitted from animals showing clinical signs of JD and sera from the US National Repository for Paratuberculosis Specimens were used to determine the sensitivity of each test. The EIA detected 48.8% of 43 Australian animals with subclinical JD, while the CFT detected only 12 (21.4%) of 56 subclinically affected cattle. Of 150 subclinically infected US cattle, the EIA detected 47.3% and the CFT detected 52.0%. The EIA detected 59.7% of animals which at the time of sampling were shedding Mycobacterium paratuberculosis in their faeces, but showed no clinical signs of JD, while the CFT detected 57.3%. The EIA correctly identified 88.2% of 136 histologically confirmed clinical cases, and the CFT detected 83.4%. The specificity of each test was determined by testing sera collected at slaughter from animals residing in a known JD-free area of Australia, and from samples from the US National Repository of Paratuberculosis Specimens collected from certified-free herds in Wisconsin. The EIA was found to have a specificity of 99.8% when 998 Australian animals were used as the test population, and 99.0% when 196 US animals were used. The specificity of the CFT using Australian samples was 96.9% and 95.2% using American samples.  相似文献   

Effects of tetracycline hydrochloride on pleurae in dogs with induced pleural effusion   总被引：1，自引：0，他引：1  

L A Gallagher  S J Birchard  S E Weisbrode 《American journal of veterinary research》1990,51(10):1682-1687

Pleural effusion was induced in 12 dogs by ligation of the cranial vena cava. Pleurodesis was attempted by injecting a solution of tetracycline hydrochloride into the pleural space of 8 dogs (4 dogs, 25 mg/kg of body weight; 4 dogs, 50 mg/kg) via bilateral thoracostomy tubes. In both groups, tetracycline was diluted in 40 ml of normal saline solution and 10 ml of 1% lidocaine before injection. Half of the solution (25 ml) was instilled in each hemithorax. Four control dogs were treated in the same manner with a solution of normal saline and lidocaine. Daily pleural fluid production was measured after the attempted pleurodesis. Thirty days after administration of the solution, each dog was euthanatized and necropsied. Surface area of pleural adhesions was measured. Tissues from regions of pleural adhesions and areas of parietal and visceral pleura not involved in adhesions were analyzed histologically. Formation of pleural fluid stopped in all but 1 control dog within 48 hours after injection of solution. This dog effused throughout the study. The resolution of effusion was not significantly (P less than 0.05) different between the tetracycline-treated dogs and the control group. Although diffuse pleural adhesions were not induced in any of the dogs, significantly (P less than 0.0027) more surface area of lung was adhered in dogs treated with the higher dose of tetracycline.  相似文献   

Radial agenesis and ulnar hypoplasia in two caprine kids     

K H Baum  B L Hull  S E Weisbrode 《Journal of the American Veterinary Medical Association》1985,186(2):170-171

Two of three caprine triplets caused dystocia due to malformed forelimbs. This malformity was caused by radial agenesis and ulnar hypoplasia. Other congenital anomalies were also present. The influence of diet versus genetics could not be ascertained. Bilateral ulnar fractures in one kid revealed histologically rounded fracture ends with prominent periosteal new bone formation.  相似文献   

Carbohydrate malabsorption in dogs assessed using bioluminescence assays to measure simple sugars     

PH BIRD  PE HARTMANN  SE KELLY  WT CLARK 《Australian veterinary journal》1990,67(9):341-342

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Corrective osteotomy for procurvatum deformity caused by distal femoral physeal fracture malunion stabilised with String‐of‐Pearls locking plates: results in two dogs and a review of the literature     

SE Kim  DD Lewis 《Australian veterinary journal》2014,92(3):75-80

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Influence of needle gauge used for venipuncture on automated platelet count and coagulation profile in dogs     

CM Greenwell  SE Epstein  PH Brain 《Australian veterinary journal》2014,92(3):71-74

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Use of helical computed tomography for measurement of thyroid glands in clinically normal cats     

Drost WT  Mattoon JS  Weisbrode SE 《American journal of veterinary research》2006,67(3):467-471

OBJECTIVE: To determine the dimensions and volume of thyroid tissue in clinically normal cats by use of computed tomography. ANIMALS: 8 cats. PROCEDURE: Helical computed tomography images (2-mm collimation) were acquired from the cranial aspect of the second cervical vertebra through the caudal aspect of the fourth cervical vertebra. Data were acquired before contrast medium administration (n = 7 cats) and immediately after contrast medium enhancement (1 cat). Length, width, and height measurements of each thyroid lobe were made by use of transverse, dorsal, and sagittal plane images. Thyroid lobe volume was estimated by use of 3 methods. RESULTS: All thyroid lobes were histologically normal. Mean dimensions for a thyroid lobe were 16.5 x 2.00 x 4.31 mm (length x width x height) using only data from transverse images. Mean thyroid lobar volume was 113.75 mm(3) using the sum of areas method. Mean total volume of thyroid tissue was 215.25 mm(3) using the sum of areas method. CONCLUSIONS AND CLINICAL RELEVANCE: Results may be useful for computed tomography evaluation of abnormal thyroid glands in cats, which warrants investigation.  相似文献   

A retrospective study of poisoning cases in dogs and cats: comparisons between a rural and an urban practice     

ID ROBERTSON  M LEGGOE  PR DORLING  SE SHAW  WT CLARK 《Australian veterinary journal》1992,69(8):194-195

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Arthropod-transmitted infectious diseases of cats     

SE Shaw  RJ Birtles  MJ Day 《Journal of Feline Medicine and Surgery》2001,3(4):193-209

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pH effects of the addition of three biochars to acidic Indonesian mineral soils     

V Martinsen  V Alling  NL Nurida  J Mulder  SE Hale  C Ritz 《Soil Science and Plant Nutrition》2013,59(5):821-834

Abstract

Soil acidity may severely reduce crop production. Biochar (BC) may increase soil pH and cation exchange capacity (CEC) but reported effects differ substantially. In a systematic approach, using a standardized protocol on a uniquely large number set of 31 acidic soils, we quantified the effect of increasing amounts (0–30%; weight:weight) of three types of field-produced BCs (from cacao (Theobroma cacao. L.) shell, oil palm (Elaeis guineensis. Jacq.) shell and rice (Oryza sativa. L.) husk) on soil pH and CEC. Soils were sampled from croplands at Java, Sumatra and Kalimantan, Indonesia. All BCs caused a significant increase in mean soil pH with a stronger response and a greater maximum increase for the cacao shell BC addition, due to a greater acid neutralizing capacity (ANC) and larger amounts of extractable base cations. At 1% BC addition, corresponding to about 30 tons ha^?1, the estimated increase in soil pH from the initial mean pH of 4.7 was about 0.5 units for the cacao shell BC, whereas this was only 0.05 and 0.04 units for the oil palm shell and rice husk BC, respectively. Besides depending on BC type, the increase in soil pH upon the addition of each of the three BCs was mainly dependent on soil CEC (low CEC resulting in stronger pH increase), and to a lesser extent on initial soil pH (higher initial pH resulting in stronger pH increase). Addition of BC also increased the amount of exchangeable base cations (cacao shell ? oil palm and rice husk) and CEC. Through this systematic screening of the effect of BC on pH and CEC of acidic soils, we show that a small addition of BC, in particular if made of cacao shell, to acidic agricultural soils increases soil pH and CEC. However, the response is highly dependent on the type, quality and amount of the added BC as well as on intrinsic soil properties, mainly CEC.  相似文献