F-actin and microtubule suspensions as indeterminate fluids   总被引：12，自引：0，他引：12  

R E Buxbaum  T Dennerll  S Weiss  S R Heidemann 《Science (New York, N.Y.)》1987,235(4795):1511-1514

The viscosity of F-actin and microtubule suspensions has been measured as a function of shear rate with a Weissenberg rheogoniometer. At shear rates of less than 1.0 per second the viscosity of suspensions of these two structural proteins is inversely proportional to shear rate. These results are consistent with previous in vivo measurements of the viscosity of cytoplasm. This power law implies that shear stress is independent of shear rate; that is, shear stress is a constant at all shear rates less than 1.0 per second. Thus the flow profile of these fluids is indeterminate, or nearly so. This flow property may explain several aspects of intracellular motility in living cells. Possible explanations for this flow property are based on a recent model for semidilute suspensions of rigid rods or a classical friction model for liquid crystals.  相似文献   

Coronavirus infection induces H-2 antigen expression on oligodendrocytes and astrocytes   总被引：8，自引：0，他引：8  

A Suzumura  E Lavi  S R Weiss  D H Silberberg 《Science (New York, N.Y.)》1986,232(4753):991-993

Infection of the central nervous system by mouse hepatitis virus strain A59, a murine neurotropic coronavirus, induces class I major histocompatibility complex antigens on mouse oligodendrocytes and astrocytes, cells that do not normally express these antigens on their surfaces. This induction, which occurs through soluble factors elaborated by infected glial cells, potentially allows immunocytes to interact with the glial cells and may play a critical role in the pathogenesis of virus-induced, immune-mediated demyelination in the central nervous system.  相似文献   

A persistent untranslated sequence within bacteriophage T4 DNA topoisomerase gene 60   总被引：30，自引：0，他引：30  

W M Huang  S Z Ao  S Casjens  R Orlandi  R Zeikus  R Weiss  D Winge  M Fang 《Science (New York, N.Y.)》1988,239(4843):1005-1012

A 50-nucleotide untranslated region is shown to be present within the coding sequence of Escherichia coli bacteriophage T4 gene 60, which encodes one of the subunits for its type II DNA topoisomerase. This interruption is part of the transcribed messenger RNA and appears not to be removed before translation. Thus, the usual colinearity between messenger RNA and the encoded protein sequence apparently does not exist in this case. The interruption is bracketed by a direct repeat of five base pairs. A mechanism is proposed in which folding of the untranslated region brings together codons separated by the interruption so that the elongating ribosome may skip the 50 nucleotides during translation. The alternative possibility, that the protein is efficiently translated from a very minor and undetectable form of processed messenger RNA, seems unlikely, but has not been completely ruled out.  相似文献   

Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line   总被引：34，自引：0，他引：34  

P Clapham  K Nagy  R Cheingsong-Popov  M Exley  R A Weiss 《Science (New York, N.Y.)》1983,222(4628):1125-1127

Human T-cell leukemia virus (HTLV), American PL isolate, was transmitted by cocultivation and by cell-free filtrates to a nonlymphoid human osteogenic sarcoma (HOS) cell line, designated HOS/PL, but not to nine other lines bearing receptors for HTLV. HOS and HOS/PL cells are not dependent on interleukin-2 and do not express interleukin-2 receptors that are recognized by anti-Tac monoclonal antibody. HTLV released by the Japanese MT2 cell line was also transmitted to HOS cells. The infected HOS cells release substantial titers of progeny HTLV which is antigenically indistinguishable from parental virus and is able to transform T cells.  相似文献   

Fluorescence spectroscopy of single biomolecules   总被引：1，自引：0，他引：1  

Weiss S 《Science (New York, N.Y.)》1999,283(5408):1676-1683

Recent advances in single-molecule detection and single-molecule spectroscopy at room temperature by laser-induced fluorescence offer new tools for the study of individual macromolecules under physiological conditions. These tools relay conformational states, conformational dynamics, and activity of single biological molecules to physical observables, unmasked by ensemble averaging. Distributions and time trajectories of these observables can therefore be measured during a reaction without the impossible need to synchronize all the molecules in the ensemble. The progress in applying these tools to biological studies with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.  相似文献   

Communications Satellites: Countdown for INTELSAT VI     

Pollack L  Weiss H 《Science (New York, N.Y.)》1984,223(4636):553-559

Since the formation of COMSAT 20 years ago, the number of international telephone circuits made through satellites has grown 400 times and the cost of a telephone call has decreased by 12,000 percent. Worldwide communications linking 109 nations are achieved with 14 satellites in earth-synchronous orbit. Advances in microwave technology have expanded the transmission bandwidth per satellite from 50 to more than 3000 megahertz. Improvements in solar cell, battery, stationkeeping, and microwave amplification technology have increased satellite lifetime from 1.5 years to 10. The sixth generation of INTELSAT satellites, now being manufactured, will be introduced into the system in 1986, and each satellite will carry more than 40,000 telephone channels plus two television programs. The next generation of satellites, now under design for expanded capacity, will be needed by the middle 1990's to meet the traffic demand. These satellites are likely to add new frequencies (20 and 30 gigahertz), onboard signal processing and switching, and more bandwidth-efficient modulation, to achieve larger communication capacity at each orbital location.  相似文献   

Risk of human immunodeficiency virus (HIV-1) infection among laboratory workers   总被引：21，自引：0，他引：21  

S H Weiss  J J Goedert  S Gartner  M Popovic  D Waters  P Markham  F di Marzo Veronese  M H Gail  W E Barkley  J Gibbons 《Science (New York, N.Y.)》1988,239(4835):68-71

In a prospective cohort study of 265 laboratory and affiliated workers, one individual with no recognized risk factors for human immunodeficiency virus type 1 (HIV-1) infection was HIV-1 seropositive at the time of entry into the study. Molecular analyses of two HIV-1 isolates derived in two independent laboratories from a blood sample from this worker showed that the isolates were indistinguishable from a genotypic form of HIV-1 present in the H9/HTLV-IIIB cell line. Exposure to this strain of virus most probably occurred during work with concentrated virus or culture fluids from virus-producing cell lines under standard Biosafety Level 3 containment. Although no specific incident leading to this infection has been identified, undetected skin contact with virus culture supernatant might have occurred. This worker was the only one found to be positive among the subgroup of 99 workers who shared a work environment involving exposure to concentrated virus. The incidence rate of 0.48 per 100 person-years exposure indicates that prolonged laboratory exposure to concentrated virus is associated with some risk of HIV-1 infection, which is comparable to the risk for health care workers experiencing a needle stick exposure. While none of the ten workers with parenteral exposure to HIV-1 in this cohort became infected, a worker in another laboratory did seroconvert following an injury with a potentially contaminated needle. Strict Biosafety Level 3 containment and practices should be followed when working with concentrated HIV-1 preparations, and further refinement of the procedures may be necessary.  相似文献   

Saturn's small inner satellites: clues to their origins     

Porco CC  Thomas PC  Weiss JW  Richardson DC 《Science (New York, N.Y.)》2007,318(5856):1602-1607

Cassini images of Saturn's small inner satellites (radii of less than approximately 100 kilometers) have yielded their sizes, shapes, and in some cases, topographies and mean densities. This information and numerical N-body simulations of accretionary growth have provided clues to their internal structures and origins. The innermost ring-region satellites have likely grown to the maximum sizes possible by accreting material around a dense core about one-third to one-half the present size of the moon. The other small satellites outside the ring region either may be close to monolithic collisional shards, modified to varying degrees by accretion, or may have grown by accretion without the aid of a core. We derived viscosity values of 87 and 20 square centimeters per second, respectively, for the ring material surrounding ring-embedded Pan and Daphnis. These moons almost certainly opened their respective gaps and then grew to their present size early on, when the local ring environment was thicker than it is today.  相似文献   

A Fluted Point from the Uptar Site, Northeastern Siberia     

ML King  SB Slobodin 《Science (New York, N.Y.)》1996,273(5275):634-636

Lanceolate bifacial points, including one fluted specimen, have been collected from beneath an early Holocene tephra at the Uptar site, northeastern Siberia. Thus, the technology associated with the well-known Paleoindian tradition was not confined to the Americas. The Uptar collection does not compare readily with other Beringian complexes and demonstrates that there is greater diversity in the archaeological record of northeastern Siberia than traditional colonization models imply.  相似文献   

CoRoT measures solar-like oscillations and granulation in stars hotter than the Sun     

Michel E  Baglin A  Auvergne M  Catala C  Samadi R  Baudin F  Appourchaux T  Barban C  Weiss WW  Berthomieu G  Boumier P  Dupret MA  Garcia RA  Fridlund M  Garrido R  Goupil MJ  Kjeldsen H  Lebreton Y  Mosser B  Grotsch-Noels A  Janot-Pacheco E  Provost J  Roxburgh IW  Thoul A  Toutain T  Tiphène D  Turck-Chieze S  Vauclair SD  Vauclair GP  Aerts C  Alecian G  Ballot J  Charpinet S  Hubert AM  Lignières F  Mathias P  Monteiro MJ  Neiner C  Poretti E  de Medeiros JR  Ribas I  Rieutord ML  Cortés TR  Zwintz K 《Science (New York, N.Y.)》2008,322(5901):558-560

Oscillations of the Sun have been used to understand its interior structure. The extension of similar studies to more distant stars has raised many difficulties despite the strong efforts of the international community over the past decades. The CoRoT (Convection Rotation and Planetary Transits) satellite, launched in December 2006, has now measured oscillations and the stellar granulation signature in three main sequence stars that are noticeably hotter than the sun. The oscillation amplitudes are about 1.5 times as large as those in the Sun; the stellar granulation is up to three times as high. The stellar amplitudes are about 25% below the theoretic values, providing a measurement of the nonadiabaticity of the process ruling the oscillations in the outer layers of the stars.  相似文献