Dietary Protein Hydrolysates from Animal By-Products: Digestibility and Enzymatic Activity for Dourado Salminus brasiliensis     

Evandro Kleber Lorenz  Rafael Simões Coelho Barone  Fernando Yugo Yamamoto 《Journal Of Aquatic Food Product Technology》2018,27(2):236-246

Fishmeal (FM) is a high-cost and scarce feedstuff. Notwithstanding, the formulation and processing of diets for carnivorous fish strongly rely on FM as a protein source, given its palatability and biological value. Animal by-product hydrolysates are high-quality feedstuff and can substitute FM in aquafeeds. This study evaluated the digestibility of hydrolysates from tilapia residue (TR), tuna head (TH), swine liver (SL), and poultry liver (PL), and the profile of digestive enzymes in juvenile dourado, Salminus brasiliensis, a carnivorous Characin, fed diets containing graded levels of the hydrolysates. Inclusion of hydrolysates in diet formulations lowered the pH of feed but did not alter feed intake by fish. Higher apparent digestibility coefficients (ADCs) of diets were recorded for fish fed diets containing TR and SL, and lower ADCs were recorded for fish fed diets containing TH. Protease and lipase activities in the animal’s stomach were higher, especially for those fed with diets containing SL. Amylase activity was higher in pyloric caeca, whereas in the intestines, the higher activity was seen in fish fed control and TR diets. Animal by-product hydrolysates were highly digestible for dourado, and the enzymatic profile of fish was influenced by the nutrient contents of diets.  相似文献   

Depletion study and estimation of withdrawal periods for florfenicol and florfenicol amine in pacu (Piaractus mesopotamicus)          下载免费PDF全文

Tamires Valim Marques  Jonas Augusto Rizzato Paschoal  Rafael Simões Coelho Barone  José Eurico Possebon Cyrino  Susanne Rath 《Aquaculture Research》2018,49(1):111-119

The intensive production of farmed fish is at a global all‐time high, and the control of bacteria proliferation in fish farms requires the frequent use of antimicrobials. This practice raises important environmental concerns related to the emergence of antimicrobial‐resistant bacteria strains. Only a few antimicrobial drugs have been approved for use in aquaculture, one of which is florfenicol. This work studies the depletion and withdrawal period of florfenicol and its main metabolite, florfenicol amine, in pacu (Piaractus mesopotamicus), a neotropical characin widely farmed in the southern hemisphere. Juvenile pacu (average weight of 724 g) were stocked in a closed‐loop laboratory system with controlled water temperature (25.8°C), and were fed for 10 consecutive days with a diet containing an intended dose of 10 mg/florfenicol per kg bw. Muscle and skin tissues were collected at 1, 3, 6, 8, 10, 12 and 16 days post‐treatment, and florfenicol and florfenicol amine were quantified using a validated ultra‐high performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS) method. The limits of quantitation for florfenicol and florfenicol amine were 10 ng/g in muscle and 50 ng/g in skin. Considering a maximum residue limit of 1000 ng/g for the sum of florfenicol and florfenicol amine in muscle with skin in natural proportions a withdrawal period of 5 days (water temperature 25.8°C) or 129 degree days was calculated on the basis of the upper limit of the one‐sided 95% confidence interval for the 99^th percentile derived from the residue depletion study.  相似文献   

Lippia alba (Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions          下载免费PDF全文

Hugo Napoleão Pereira da Silva  Elen Monique de Oliveira Sousa  Janna Laely dos Santos Maia  Mônica Tatiane Lima Pinheiro  Soraia Valeria de Oliveira Coelho Lameirão  Rosa Helena Veras Mourão  José Guilherme Soares Maia  Bernardo Baldisserotto  Lenise Vargas Flores da Silva 《Aquaculture Research》2018,49(1):128-134

Lippia alba hydrolate (LAH) is produced as a secondary product in essential oil extraction. The objective of this study was to evaluate the use of LAH in a simulation of the transport of tambaqui (Colossoma macropomum) juveniles. A series of 17 hr simulations were performed using load densities of 30, 60 and 90 juveniles per L and four LAH concentrations of 0.4%, 1%, 2% and 5%, with a control of just water. Fish survival, temperature, dissolved oxygen, conductivity, pH, ammonia excretion, alkalinity water hardness and net Na⁺, K⁺, Cl^‐ fluxes, were all evaluated before and after the transport simulations. Mucous cell densities in the gills were also determined microscopically, post simulation. The results showed that addition of 5% LAH achieved better survival during the simulation than the water control. Dissolved oxygen was significantly lower with an increased load density. Also, conductivity, NH₄ excretion and net ionic fluxes (K⁺ and Cl^?) were lower with 5% LAH, although mainly where higher load densities were tested. Mucous cell density was lower in LAH concentrations of 2% and 5%, again with higher load densities. In view of these findings, it is suggested that 5% LAH is an effective sedative for use in the transport of tambaqui, mainly with high load densities. This work is the first study of LAH and highlights its potential applications in aquaculture management.  相似文献   

Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein     

André Gomes Coelho Souza  Sonia Herrero  Luiz Antonio Maffia  Margaret Elizabeth Daub 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(2):235-238

Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈10⁸/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %).  相似文献   

Evaluation of the molecular response of corpora lutea to manganese–amino acid complex supplementation in gilts     

Jamie M Studer  Zoe E Kiefer  Brady M Goetz  Aileen F Keating  Lance H Baumgard  Zachary J Rambo  Wesley P Schweer  Mark E Wilson  Christof Rapp  Jason W Ross 《Journal of animal science》2021,99(9)

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Establishment of a proteomic reference map for the gastrocnemius muscle in the rabbit (Oryctolagus cuniculus)     

Andr M. Almeida  Alexandre Campos  Sofia van Harten  Luís Alfaro Cardoso  Ana Varela Coelho 《Research in veterinary science》2009,87(2):196-199

In several laboratory and production species, the establishment of a proteome reference map of a specific tissue has been accomplished. The rabbit is widely used as both a production and experimental animal. A lot of physiology research involving the gastrocnemius muscle of rabbit is described, although no reference proteome map is available. In this work, the first reference map of the rabbit’s gastrocnemius muscle using 2D gel electrophoresis and the identification of proteins through peptide mass fingerprinting (PMF) was established. A total of 45 proteins were localized and identified with three major roles: cell structure and contractile apparatus; metabolic and cell defense proteins. A reference map of major proteins expressed is described enabling possible comparisons with other physiological studies.  相似文献   

Australian surveillance for avian influenza viruses in wild birds between July 2005 and June 2007   总被引：1，自引：1，他引：0  

L Haynes  E Arzey  C Bell  N Buchanan  G Burgess  V Cronan  C Dickason  H Field  S Gibbs  PM Hansbro  T Hollingsworth  AC Hurt  P Kirkland  H McCracken  J O'Connor  J Tracey  J Wallner  S Warner  R Woods  C Bunn 《Australian veterinary journal》2009,87(7):266-272

Objective   To identify and gain an understanding of the influenza viruses circulating in wild birds in Australia.
Design   A total of 16,303 swabs and 3782 blood samples were collected and analysed for avian influenza (AI) viruses from 16,420 wild birds in Australia between July 2005 and June 2007. Anseriformes and Charadriiformes were primarily targeted.
Procedures   Cloacal, oropharyngeal and faecal (environmental) swabs were tested using polymerase chain reaction (PCR) for the AI type A matrix gene. Positive samples underwent virus culture and subtyping. Serum samples were analysed using a blocking enzyme-linked immunosorbent assay for influenza A virus nucleoprotein.
Results   No highly pathogenic AI viruses were identified. However, 164 PCR tests were positive for the AI type A matrix gene, 46 of which were identified to subtype. A total of five viruses were isolated, three of which had a corresponding positive PCR and subtype identification (H3N8, H4N6, H7N6). Low pathogenic AI H5 and/or H7 was present in wild birds in New South Wales, Tasmania, Victoria and Western Australia. Antibodies to influenza A were also detected in 15.0% of the birds sampled.
Conclusions   Although low pathogenic AI virus subtypes are currently circulating in Australia, their prevalence is low (1.0% positive PCR). Surveillance activities for AI in wild birds should be continued to provide further epidemiological information about circulating viruses and to identify any changes in subtype prevalence.  相似文献   

Ecosystem services: Foundations,opportunities, and challenges for the forest products sector   总被引：2，自引：0，他引：2  

Trista M. Patterson  Dana L. Coelho 《Forest Ecology and Management》2009

The ecosystem service concept has been proposed as a meaningful framework for natural resource management. In theory it holds concomitant benefit and consequence for the forest product sector. However, numerous barriers impede practitioners from developing concrete and enduring responses to emerging ecosystem service markets, policies, and initiatives. Principle among these barriers is that the ecosystem service concept has a complex history, numerous definitions in use, and an astounding diversity in rationale and application. This article provides a conceptual review of ecosystem services and its economic foundations, distinguishes among several current definitions of the term and their relatedness to strategies in practical application, discusses diverse approaches to valuation, and explores potential for future relevance in forest product and other sectors.  相似文献   

Effects of 17/0 circle hooks and bait on fish catches in a Southern Atlantic swordfish longline fishery          下载免费PDF全文

Sérgio Amorim  Miguel N. Santos  Rui Coelho  Joana Fernandez‐Carvalho 《水产资源保护:海洋与淡水生态系统》2015,25(4):518-533

Notes on Propagation of Butterfly Peacock Bass Cichla ocellaris (Bloch & Schneider, 1801) in a Tropical Fish Hatchery          下载免费PDF全文

J Wesley Neal  Karina M Olivieri‐Velázquez 《Aquaculture Research》2015,46(7):1785-1787

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