Superovulatory Response of Zebu Cows Treated with pFSH in a Single Subcutaneous Injection Followed by an Additional Intramuscular Sub‐Dose 48 h Later     

RH Alvarez  AC Martinez  RML Pires 《Reproduction in domestic animals》2010,45(3):421-424

The aim of this study was to evaluate whether an additional intramuscular (im) injection of pFSH would increase the embryo production in zebu cows superovulated with a single subcutaneous (sc) pFSH injection. Twenty‐one Nelore cows were treated with a progesterone vaginal implant (Controlled Internal Drug Relased – CIDR B^®) and injected im with 2.5 mg estradiol benzoate. Four days later, cows were assigned randomly into three groups and superovulated with pFSH. Groups A and B received single sc injections of 400 and 320 IU, respectively; Group C received multiple im injections of 400 IU in decreasing doses at 12‐h intervals over 4 days. In the morning (07:00 am) of day 3 after starting superovulation, cows received im 150 mcg cloprostenol and Group B was additionally injected im with 80 IU of pFSH. CIDR‐B was withdrawn in the afternoon (07:00 pm). Cows were inseminated 48 and 62 h after the cloprostenol injection. Embryo collection and corpora lutea (CL) estimation were done 7 days after insemination. Alternation of treatments (crossover design) occurred at a 60‐day interval. There was no significant difference (p > 0.05) of CL counts among treatments. The total (transferable and no transferable) number of recovered embryos from Group A (6.9 ± 1.5) was not different from Group C (9.8 ± 1.2), whereas Group B (5.7 ± 1.5) was lower than Group C (p < 0.05). The number of transferable embryos from Groups A (2.4 ± 0.7) and B (1.7 ± 0.6) was lower (p < 0.05) than Group C (4.6 ± 1.2). Lesser (p < 0.05) embryo production from Group B was related to lower recovery rate (46.4%), compared with Groups A (65.1%) and C (81.7%). It was concluded that an additional im subdose of pFSH, injected 48 h after a single subcutaneous (sc) dose of pFSH, does not improve the embryo production in zebu cows.  相似文献   

Morpho‐physical Recording of Bovine Conceptus (Bos indicus) and Placenta from Days 20 to 70 of Pregnancy     

AC Assis Neto  FTV Pereira  TC Santos  CE Ambrosio  R Leiser  MA Miglino 《Reproduction in domestic animals》2010,45(5):760-772

The study is based on 141 pregnant Bos indicus cows, from days 20 to 70 post‐insemination. First, special attention was given to the macroscopically observable phenomena of attachment of the conceptus to the uterus, i.e. the implantation, from about days 20 to 30 post‐insemination up to day 70, and placentome development by growth, vascularization and increase in the number of cotyledons opposite to the endometrial caruncles. Secondly, as for the conceptuses, semiquantitative, statistical analyses were performed of the lengths of chorio‐allantois, amnion and yolk sac; and the different parts of the centre and two extremes of the yolk sacs were also analysed. Thirdly, the embryos/foetuses corresponding to their membranes were measured by their greatest length and by weight, and described by the appearance of external developmental phenomena during the investigated period like neurulation, somites, branchial arcs, brain vesicles, limb buds, C‐form, pigmented eye and facial grooves. In conclusion, all the data collected in this study from days 20 to 70 of bovine pregnancy were compared extensively with corresponding data of the literature. This resulted in an ‘embryo/foetal age‐scale’, which has extended the data in the literature by covering the first 8 to 70 days of pregnancy. This age‐scale of early bovine intrauterine development provides model for studies, even when using slaughtered cows without distinct knowledge of insemination or fertilization time, through macroscopic techniques. This distinctly facilitates research into the cow, which is now being widely used as ‘an experimental animal’ for testing new techniques of reproduction like in vitro fertilization, embryo transfer and cloning.  相似文献   

Persistent Mullerian Duct Syndrome in a Miniature Schnauzer Dog with Signs of Feminization and a Sertoli Cell Tumour     

AR Vegter  HS Kooistra  FJ Van Sluijs  LWL Van Bruggen  J Ijzer  C Zijlstra  AC Okkens 《Reproduction in domestic animals》2010,45(3):447-452

A 5‐year‐old male Miniature Schnauzer was presented with unilateral cryptorchidism and signs of feminization. Abdominal ultrasonography revealed an enlarged right testis and a large, fluid‐filled cavity that appeared to arise from the prostate. Computed tomography revealed the cavity to be consistent with an enlarged uterine body, arising from the prostate, and showed two structures resembling uterine horns that terminated close to the adjacent testes. The dog had a normal male karyotype, 78 XY. Gonadohysterectomy was performed and both the surgical and the histological findings confirmed the presence of a uterus in this male animal, resulting in a diagnosis of persistent Mullerian duct syndrome (PMDS). The enlarged intra‐abdominal testis contained a Sertoli cell tumour. Computed tomography proved to be an excellent diagnostic tool for PMDS.  相似文献   

Management of strawberry anthracnose by choice of irrigation system,mulching material and host resistance     

Marcus V.S. Coelho  Flávio R. Palma 《国际虫害防治杂志》2013,59(4):347-354

We studied, by means of field experiments, the combined effects of irrigation system, mulching material and genetic resistance on the incidence of strawberry anthracnose caused by Colletotrichum acutatum. All trials involved artificial inoculation with fruits colonized by the pathogen. One experiment examined the effects of drip irrigation, overhead sprinkler irrigation, and localized low-pressure tape irrigation, and the effects of grass (Brachiaria sp.) and plastic (polyethylene) mulches, in a split plot, randomized complete block design with four replicates. Lower disease incidence, indicating lower pathogen dispersal, was verified with low-pressure tape and drip irrigation. Flower blight and fruit rot incidence in drip-irrigated plots was minimal (ca. 2% of the disease levels in the plots otherwise irrigated). Grass mulch reduced flower blight in the sprinkler system (up to 80%), but had no significant effect when disease levels were already low due to use of other irrigation systems. A second experiment compared the effects of grass mulch, pine (Pinus elliotti) mulch, and plastic mulch with overhead sprinkler irrigation on strawberry cultivars partially resistant (‘Dover’) and susceptible (‘Campinas’) to the disease in a factorial completely randomized design (CRD). Both organic mulches reduced flower blight and diseased fruit incidence by up to 76% compared to the plastic mulch treatment, especially in the early stages of the epidemics. ‘Dover’ had 70% lower incidence of flower blight than ‘Campinas’, and the effects of organic mulches were more pronounced in ‘Campinas’. A third experiment, with all three irrigation systems combined with inoculated and non-inoculated plots, estimated yield effects due to disease and irrigation system. All plots were covered with plastic mulch in a CRD with six treatments. This experiment clearly confirmed the higher flower blight and fruit rot incidences when sprinkler irrigation was used. In inoculated treatments, fruit yield was significantly reduced in tape-irrigated plots, and more so in sprinkler-irrigated plots. In drip-irrigated plots yield was reduced by only 1%. Overall, our results confirm the importance of cultural and genetic factors as valuable means of strawberry anthracnose management. Adoption of localized irrigation systems, the use of organic mulches and choice of a partially resistant cultivar significantly reduced disease levels in field plots. The importance of water splash for C. acutatum dispersal and its dependency on the soil mulch characteristics are the likely causes for the results observed.  相似文献   

Removal of Arsenic from Aqueous Solutions by Sorption onto Sewage Sludge-Based Sorbent   总被引：1，自引：0，他引：1  

Daniela S. Tavares  Cláudia B. Lopes  Jo?o P. Coelho  Marta E. Sánchez  Ana I. Garcia  Armando C. Duarte  Marta Otero  Eduarda Pereira 《Water, air, and soil pollution》2012,223(5):2311-2321

This study aimed at evaluating and comparing the removal of arsenic from solutions by a low-cost waste-based sorbent, produced by pyrolysing sewage sludge under appropriate conditions, and by a commercially activated carbon. Batch sorption experiments were performed under isothermal conditions (20°C), in order to evaluate the effect of pH on the arsenic sorption kinetics and on the equilibrium sorption capacity of the materials under study. Kinetic data revealed that the arsenic sorption was faster onto the activated carbon than onto the pyrolysed sludge. The sorption process was well described by both the pseudo-first and pseudo-second-order kinetics equations for both materials. Changes in the initial solution pH have distinct effects on the removal of arsenic onto pyrolysed sludge and activated carbon. While for pyrolysed sludge, pH affects essentially the equilibrium time, for activated carbon it affects the sorption capacity. Equilibrium results were well described by both Freundlich and Langmuir isotherm models, although fittings corresponding to the Langmuir isotherm were slightly better. The Langmuir maximum sorption capacity determined for the pyrolysed sludge was 71???g?g^?1, while for activated carbon was 229???g?g^?1. Despite the relative lower capacity of the pyrolysed sludge, the considerable lower cost and the valorisation of the sludge may justify further research on its use for water decontamination.  相似文献   

Radiographic quantitative assessment of caudal proximal tibial angulation in 100 dogs with cranial cruciate ligament rupture     

Glassman M  Hofmeister E  Weh JM  Roach W  Torres B  Johnston S  Budsberg S 《Veterinary surgery : VS》2011,40(7):830-838

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Molecular detection of Leishmania sp. in cats (Felis catus) from Andradina Municipality, São Paulo State, Brazil     

Coelho WM  Richini-Pereira VB  Langoni H  Bresciani KD 《Veterinary parasitology》2011,176(2-3):281-282

The aim of this work was to molecularly detect Leishmania species in 52 cats from Andradina Municipality, S?o Paulo State, Brazil. The direct parasitological test was performed by using imprints of poplited lymph node, bone marrow and spleen to verify amastigote forms of Leishmania spp. The samples that were positive parasitological tests were subjected to molecular analysis (PCR) and sequencing. Infection was detected for 5.76% (3/52) of the examined cats and two had presence of amastigote forms of Leishmania spp. in lymph nodes. Polymerase chain reaction (PCR) of kinetoplast minicircle DNA, indicated positive amplification for samples of spleen and lymph nodes and the sequencing resulted in 97% similarity with Leishmania (L.) chagasi. This study proved the occurrence of infection with Leishmania (L.) chagasi in felines from Andradina municipality, S?o Paulo State.  相似文献   

Biomass,Mineral Accumulation,and Calcium Crystals in Trema micrantha (L.) Blume as a Function of Calcium Carbonate Addition     

Janete Mariza Adamski  Geraldo Ceni Coelho 《Journal of plant nutrition》2013,36(2):205-217

Trema micrantha (L.) Blume is a neotropical multipurpose tree species, which could be used in forest restoration and agroforestry. Several cultures, however, require liming to satisfactory yields in acid soils. Therefore, it is important to determine the effect of liming to this native species, in order to include it in agroforestry associations. The aim of this work was to evaluate the morphological changes of T. micrantha, including the calcium oxalate (druses) and cystolith density, as caused by the calcium carbonate (CaCO₃) addition. The assay included three treatments: control, pH 5.3; treatment 1, pH 5.5; treatment 2, pH 6.0. The liming reduced the root biomass and increased the shoot biomass. The leaf specific mass and nutrients concentration were not changed. The leaves presented higher density of druses in treatment 2. The cystolith density was not modified, but the cystolith size was increased by the liming. The results indicate liming tolerance in this species.  相似文献   

Stem dimensional fluctuation in Jeffrey pine from variation in water storage as influenced by thinning and prescribed fire     

Robert M. Fecko  Roger F. Walker  Wesley B. Frederick  Watkins W. Miller  Dale W. Johnson 《Annals of Forest Science》2008,65(2):201-201

Forest thinning utilizing cut-to-length and whole-tree harvesting systems with subsequent underburning were assessed for their impacts on water storage in the extensible tissues of dominant and codominant trees in an uneven-aged Jeffrey pine (Pinus jeffreyi Grev. & Balf.) stand on the east slope of the Sierra Nevada. Prior to the onset of the third growing season following thinning and the second season after burning, manual band dendrometers were installed at breast height on the selected trees and readings of diurnal fluctuation in stem circumference, an indication of bole water status, were taken monthly for one year. Diameter and relative diameter fluctuation were calculated from the circumference measurements. Overall, thinning had a positive influence on stem water recharge capacity, with the most pronounced effects evident in the latter part of the growing season. During this period, bole contraction in thinned stand portions was 49 to 55% greater than in the unthinned control, suggesting that both a greater volume of stored water was available for transpiration and was transpired in trees of the former treatment. There was no clear evidence that harvesting method affected stem water storage and influences of underburning were also absent entirely. Seasonal effects on diurnal changes in stem diameter were prominent, as the extent to which boles contracted generally increased over the course of the growing season, whereas fluctuations were at a minimum during the colder months. The magnitude of stem dimensional flux was found to be negatively correlated with initial tree DBH in one instance, while negative relationships between the former and live crown length as well as percentage were also revealed, albeit infrequently. Changes in bole size were positively correlated with residual basal area in some cases. These results suggest that improvement in water relations can be realized from density management in a dry site forest type with no apparent compromise of this benefit by broadcast underburning.  相似文献   

Comparing the value of Southern Oscillation Index-based climate forecast methods for Canadian and US wheat producers     

Harvey S. J. Hill  Graduate Research Assistant  Jaehong Park  Graduate Research Assistant  James W. Mjelde  Professor  Wesley Rosenthal  Assistant Professor  H. Alan Love  Associate Professor  Stephen W. Fuller  Professor 《Agricultural and Forest Meteorology》2000,100(4)

Southern Oscillation Index (SOI) based forecasting methods are compared to determine which method is more valuable to Canadian and US wheat producers. Using decision theory approach to valuing information, the more commonly used three-phase method of El Niño, La Niña, and other is compared to a five-phase system. Because of differences in growing season and yearly SOI classification schemes, two different three-phase methods are used. The five-phase system is based on the level and rate of change of the SOI over a 2 month period. Phases are consistently negative, consistently positive, rapidly falling, rapidly rising, and near zero. As expected, results vary by the method used. Winter wheat producers in Illinois place no value on either of the SOI-based forecasting systems. Producers at seven of the 13 sites prefer the five-phase method over either of the three-phase method (spring wheat producers in Manitoba, Alberta, North Dakota and South Dakota, along with winter wheat producers in Oklahoma, Texas, and Washington). The value of the five-phase approach is up to 70 times more valuable than the three-phase approach. Producers growing spring wheat in Saskatchewan and Montana, along with winter wheat producers in Ohio and Kansas value the three-phase approach more than the five-phase. In this case, the value of the three-phase system is up to two times more valuable than the five-phase system. Depending on expected price and region, the values of the SOI-based forecasts range from 0 to 22% of the value of perfect forecasts. In both absolute and percentage of perfect forecasts, producers in Oklahoma, Texas, Manitoba, Saskatchewan, and South Dakota value either system more than producers in the remaining regions. Economic value and distributional aspects of the value of climate forecasts have implications for producers, policy makers, and meteorologists. Finally, the results clearly suggest all producers will not prefer one forecast type. Forecasts need to be tailored to specific regions.  相似文献