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71.
72.
AIM: To investigate the anthelmintic properties of New Zealand native flax (Phormium tenax) for cattle, using a faecal egg count reduction test (FECRT).

METHODS: Twenty-six heifer calves with high (>300 epg) faecal nematode egg counts (FEC) were paired into two groups and fed either chopped flax leaves ad libitum, or barley straw at an equivalent dry matter (DM) rate, from Day 0–7; all were supplemented with 1.5 kg/head/day cereal feed supplement. On Days 8–10, all heifers were fed a common diet of 3.5 kg DM/ head/day barley straw and 1.5 kg/head/day cereal feed supplement. Heifers were weighed and faecal samples were collected on Days —6, 0, 7, and 10; samples were analysed in triplicate for FEC. The nutritive value of the flax and straw was analysed.

RESULTS: Both the flax and straw had low dry matter digestibility (DMD) and protein content. Although the flax-fed claves grew more than the controls, this may have been related to gut fill. Total daily faecal egg output was similar for flax and straw groups on Day 7 (14.7 vs 15.0 x 106 eggs/day, respectively) and Day 10 (14.9 vs 15.1 x 106 eggs/day, respectively). There was no difference in the change in FEC with time between the calves fed flax or straw diets.

CONCLUSION: Consumption of flax leaves did not reduce FEC in calves with a mixed nematode infection.  相似文献   
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The bacterial load and degree of antibiotic resistance present in untreated and antibiotic‐treated semen samples were investigated in five bulls standing at a cattle‐breeding centre. Bacterial load was determined by colony counts from semen samples cultured on brain heart infusion and nutrient agar plates. Antibiotic resistance in these bacteria was assessed by measuring the diameter of bacterial growth inhibition zones around discs containing different concentrations of antibiotics. Representative antibiotic‐resistant bacterial isolates were selected for identification. Untreated semen contained few culturable bacteria, and all were completely sensitive to gentamycin, spectinomycin and lincomycin: six of the isolates showed some resistance to tylosin. In semen to which antibiotics had been added as part of the routine production process, two isolates were sensitive to all of the antibiotics tested, and the remainder were resistant to all. Resistant Gram‐negative isolates that were identified included Pseudomonas and Stenotrophomonas spp. both in the class Gammaproteobacteria and a Sphingomonas sp. which is in the class Alphaproteobacteria.  相似文献   
75.
Abstract

The effects of aerosol dosages of different categories of insecticide were tested against Glossina tachinoides when applied by helicopter to riverine forest habitats of the River Komoe, Upper Volta, West Africa. The method of application, droplet characteristics of the aerosols and meteorological conditions are described in accompanying papers. Comparative trials based on field dosages calculated initially from dosage mortality curves obtained from topical application studies in the laboratory were carried out. Endosulfan applied at 5.4 g a.i./ha and 9.0 g a.i./ha reduced G. tachinoides populations (determined by Challier/Laveissière traps) by more than 90% in all age and sex categories and evidence is presented which indicates a residual effect at the higher of these dosages. Good results were also obtained with the synthetic pyrethroid decamethrin at a dosage of 0.36 g a.i./ha; another synthetic pyrethroid, permethrin, gave promising results at a dosage of 1.9 g a.i./ha. At the higher dosage of 4.3 g a.i./ha disappointing results were obtained, which were considered to be more attributable to a weakness in the application technique under challenging field conditions, than to a limitation of the insecticide. The organophosphate compounds tested, fenthion, azamethiphos and tetrachlorvinphos although known from laboratory tests to be appreciably toxic to G. tachinoides, performed poorly under field conditions. This was thought to be due to the formulations employed and the authors feel that further small-scale field testing of different formulations of these compounds is warranted. The compounds considered most worthy of larger scale field evaluation were endosulfan and permethrin. Although decamethrin was also very toxic to G. tachinoides it was considered inadvisable to field test this compound on a larger scale, until more information was available on its impact on aquatic food chains. The trials demonstrated that it is not always possible to extrapolate from laboratory-obtained comparative dosage/mortality data (in ng/fly) the field dosages of insecticides (in g a.i./ha) required to produce a certain level of mortality in target populations of G. tachinoides.  相似文献   
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Several outbreaks of anaemia, jaundice, abortion and mortality in cattle in New South Wales were attributed to the intracellular parasite, Theileria buffeli . Disease occurred predominantly in periparturient animals that had been moved from inland to coastal areas. Diagnosis was made via exclusion of other causes of haemolytic anaemia and observation of the parasite in blood smears. Treatments included both registered and non-registered products. There is a possibility of a new strain of Theileria sp. in Australia and the possible vectors encountered in NSW are discussed.  相似文献   
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The objective of this study was to evaluate the quality of chilled dog semen processed with extenders containing various concentrations of N‐acetyl‐L‐cysteine (NAC). Ejaculates from five dogs were collected, pooled and evaluated for concentration, motility, rapid steady forward movement (RSF‐movement), viability, acrosomal integrity and by the hypo‐osmotic swelling test (HOST). In addition, superoxide anion (O2‐?) production, hydroxyl radicals (OH?) and total reactive oxygen species (tROS) were determined. The pool was divided into five aliquots, which were diluted to a final concentration of 66.66 × 106 spermatozoa/ml with Tris‐glucose‐egg yolk extender containing one of the following concentrations of NAC (0, 0.5, 1, 2.5 or 5 mm ). The semen aliquots were chilled and preserved at 4°C. Semen quality was evaluated after rewarming at 72 h. Sperm motility was significantly higher with the 0.5 mm concentration compared with the control group (p = 0.001). Rapid steady forward movement was higher with the 0.5 and 1 mm concentrations compared with the control and 5 mm group (p < 0.001). Viability and HOST percentages were not significantly altered. Compared with the control, the 5 mm concentration showed significantly reduced percentages of spermatozoa with normal acrosomes (p = 0.049). None of the ROS values at 72 h were significantly affected by the presence of NAC in semen extenders, although all NAC concentrations showed lower O2‐? and OH? values compared with the control. Only the concentrations of 1 and 5 mm inhibited the significant increase of tROS values after 72 h, compared with the fresh semen value. In conclusion, NAC supplementation of semen extenders is beneficial to semen motility of canine spermatozoa during chilling with the 0.5 mm concentration being the most effective, although no significant ROS inhibition was observed at 72 h.  相似文献   
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