Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch   总被引：1，自引：0，他引：1  

Lando D  Peet DJ  Whelan DA  Gorman JJ  Whitelaw ML 《Science (New York, N.Y.)》2002,295(5556):858-861

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Using Cs measurements to validate the application of the AGNPS and ANSWERS erosion and sediment yield models in two small Devon catchments     

D. E. Walling  Q. He  P. A. Whelan 《Soil & Tillage Research》2003,69(1-2):27-43

Distributed erosion and sediment yield models are being increasingly used for predicting soil erosion and sediment yields in agricultural catchments. In most applications, validation of such models has commonly been restricted to comparison of the predicted and measured sediment output from a catchment, because spatially distributed information on rates and patterns of soil redistribution within the catchment has been lacking. However, such spatially distributed data are needed for rigorous model testing, in order to validate the internal functioning of a model and its applicability at different spatial scales. The study reported in this paper uses two approaches to test the performance of the agricultural non-point source pollution (AGNPS) and areal non-point source watershed environmental response simulation (ANSWERS) erosion and sediment yield models in two small catchments in Devon, UK. These involve, firstly, comparison of observed and predicted runoff and sediment output data for individual storm events monitored at the basin outlets and, secondly, information on the spatial pattern of soil redistribution within the catchments derived from ¹³⁷Cs measurements. The results obtained indicate that catchment outputs simulated by both models are reasonably consistent with the recorded values, although the AGNPS model appears to provide closer agreement between observed and predicted values. However, the spatial patterns of soil redistribution and the sediment delivery ratios predicted for the two catchments by the AGNPS and ANSWERS models differ significantly. Comparison of the catchment sediment delivery ratios and the pattern of soil redistribution in individual fields predicted by the models with equivalent information derived from ¹³⁷Cs measurements indicates that the AGNPS model provides more meaningful predictions of erosion and sediment yield under UK conditions than the ANSWERS model and emphasises the importance of using information on both catchment output and sediment redistribution within the catchment for model validation.  相似文献   

Potential Reservoirs and Risk Factors for VHSV IVb in an Enzootic System: Budd Lake,Michigan     

Elizabeth Throckmorton  Travis Brenden  Amber K. Peters  Tammy J. Newcomb  Gary E. Whelan  Mohamed Faisal 《Journal of aquatic animal health》2013,25(1):31-42

Abstract

Viral hemorrhagic septicemia virus genotype IVb (VHSV IVb) has caused major, sporadic fish die-offs in the Laurentian Great Lakes region of North America since 2005. Presently, factors affecting VHSV IVb persistence in enzootic systems are not well understood. Even with annual surveillance, the virus can go undetected for several years after an outbreak before again re-emerging, which suggests that the virus is maintained in the system either below detectable levels or in untested reservoirs. The aim of this study was to identify potential reservoirs of VHSV IVb in Budd Lake, Michigan; VHSV IVb was first detected in Budd Lake in 2007 but remained undetected until 2011. Additionally, we explored the susceptibility of naive fish introduced into a water body enzootic for VHSV IVb by stocking age-0 Largemouth Bass Micropterus salmoides at varying densities into enclosures in the lake. The virus was not detected among samples of the fishes Notropis spp. and Lepomis spp., cylindrical papershell mussels Anodontoides ferussacianus, leeches (subclass Hirudinea), sediment, or water. However, the virus was successfully isolated from amphipods (family Hyalellidae) and Largemouth Bass held in the enclosures. Our finding of VHSV IVb in Hyalellidae amphipods in combination with other research that has detected the virus in Diporeia spp., a large benthic amphipod important as a food resource to Great Lake fishes, suggests that benthic macroinvertebrates are a reservoir for VHSV IVb in infected systems. If there are environmental reservoirs for VHSV IVb in infected systems, they are likely unevenly distributed. Findings of this study add to our understanding of the seemingly complex ecology of this deadly and economically detrimental virus.

Received February 22, 2016; accepted October 16, 2016 Published online February 6, 2017  相似文献   

Impact of fire regimes,logging and topography on hollows in fallen logs in eucalypt forest of south eastern Australia     

Luke Collins  Ross A. Bradstock  Elizabeth M. Tasker  Robert J. Whelan 《Biological conservation》2012,149(1):23-31

Log hollows provide important habitat for a range of vertebrate fauna. Despite this, little is known about the impact fire regimes have on this resource, or the role topography may play in preserving hollows through the mitigation of fire intensity. This study examined the effect different combinations of fire frequency and topography have on (i) the number of hollow bearing logs and (ii) the presence and size of hollows within logs. The influence of wildfire severity and logging were also examined. Hollow availability (i.e. density of hollow bearing logs and hollow presence within logs) was greatest at sites burnt at ‘low’ frequency. The density of hollow logs was greater in gullies, though the effect of fire frequency did not vary with topographic position. Hollows showing signs of internal fire charring typically had a greater entrance width than unburnt hollows, which suggests fire plays an important role in creating large hollows. The number of hollow bearing logs increased with logging intensity, due to unmerchantable timber being left in situ. Wildfire severity had little effect on log hollows. The results indicate that frequent burning may reduce hollow availability, though it is likely that gullies will still retain a high density of hollow bearing logs irrespective of burning, and may play an important role in preserving connectivity of this resource across landscapes.  相似文献   

Evaluation of a local regression kriging approach for mapping apparent electrical conductivity of soil (ECa) at high resolution     

Wei Sun  Brett M. Whelan  Budiman Minasny  Alex B. McBratney 《植物养料与土壤学杂志》2012,175(2):212-220

Apparent electrical conductivity of soil (ECa) is a property frequently used as a diagnostic tool in precision agriculture, and is measured using vehicle‐mounted proximal sensors. Crop‐yield data, which is measured by harvester‐mounted sensors, is usually collected at a higher spatial density compared to ECa. ECa and crop‐yield maps frequently exhibit similar spatial patterns because ECa is primarily controlled by the soil clay content and the interrelated soil moisture content, which are often significant contributors to crop‐yield potential. By quantifying the spatial relationship between soil ECa and crop yield, it is possible to estimate the value of ECa at the spatial resolution of the crop‐yield data. This is achieved through the use of a local regression kriging approach which uses the higher‐resolution crop‐yield data as a covariate to predict ECa at a higher spatial resolution than would be prudent with the original ECa data alone. The accuracy of the local regression kriging (LRK) method is evaluated against local kriging (LK) and local regression (LR) to predict ECa. The results indicate that the performance of LRK is dependent on the performance of the inherent local regression. Over a range of ECa transect survey densities, LRK provides greater accuracy than LK and LR, except at very low density. Maps of the regression coefficients demonstrated that the relationship between ECa and crop yield varies from year to year, and across a field. The application of LRK to commercial scale ECa survey data, using crop yield as a covariate, should improve the accuracy of the resultant maps. This has implications for employing the maps in crop‐management decisions and building more robust calibrations between field‐gathered soil ECa and primary soil properties such as clay content.  相似文献   

Effect of Different Combinations of Cryoprotectants on In Vitro Maturation of Immature Buffalo (Bubalus bubalis) Oocytes Vitrified by Straw and Open‐Pulled Straw Methods     

KGhM Mahmoud  TH Scholkamy  YF Ahmed  GE Seidel Jr  MF Nawito 《Reproduction in domestic animals》2010,45(4):565-571

This study was designed to evaluate effects of different combinations of cryoprotectants on the ability of vitrified immature buffalo oocytes to undergo in vitro maturation. Straw and open‐pulled straw (OPS) methods for vitrification of oocytes at the germinal vesicle stage also were compared. The immature oocytes were harvested from ovaries of slaughtered animals and were divided into three groups: (i) untreated (control); (ii) exposed to cryoprotectant agents (CPAs); or (iii) cryopreserved by straw and OPS vitrification methods. The vitrification solution (VS) consisted of 6 m ethylene glycol (EG) as the standard, control vitrification treatment, and this was compared with 3 m EG + 3 m dimethyl sulfoxide (DMSO), 3 m EG + 3 m glycerol, and 3 m DMSO + 3 m glycerol. Cryoprotectants were added in two steps, with the first step concentration half that of the second (and final) step concentration. After warming, oocyte samples were matured by standard methods and then fixed and stained for nuclear evaluation. Rates of MII oocytes exposed to CPAs without vitrification were lower (54.3 ± 1.9% in EG, 47.5 ± 3.4% in EG + DMSO, 36.8 ± 1.2% in EG + glycerol and 29.9 ± 1.0% in DMSO + glycerol; p < 0.05) than for the control group (79.8 ± 1.3%). For all treatments in each vitrification experiment, results were nearly identical for straws and OPS, so all results presented are the average of these two containers. The percentages of oocytes reaching telophase‐I or metaphase‐II stages were lower in oocytes cryopreserved using all treatments when compared with control. However, among the vitrified oocytes, the highest maturation rate was seen in oocytes vitrified in EG + DMSO (41.5 ± 0.6%). Oocytes cryopreserved in all groups with glycerol had an overall low maturation rate 19.0 ± 0.6% for EG + glycerol and 17.0 ± 1.1% for DMSO + glycerol. We conclude that the function of oocytes was severely affected by both vitrification and exposure to cryoprotectants without vitrification; the best combination of cryoprotectants was EG + DMSO for vitrification of immature buffalo oocytes using either straw or OPS methods.  相似文献   

Tunneling into a single magnetic atom: spectroscopic evidence of the kondo resonance     

V Madhavan  W Chen  T Jamneala  MF Crommie  NS Wingreen 《Science (New York, N.Y.)》1998,280(5363):567-569

The Kondo effect arises from the quantum mechanical interplay between the electrons of a host metal and a magnetic impurity and is predicted to result in local charge and spin variations around the magnetic impurity. A cryogenic scanning tunneling microscope was used to spatially resolve the electronic properties of individual magnetic atoms displaying the Kondo effect. Spectroscopic measurements performed on individual cobalt atoms on the surface of gold show an energetically narrow feature that is identified as the Kondo resonance-the predicted response of a Kondo impurity. Unexpected structure in the Kondo resonance is shown to arise from quantum mechanical interference between the d orbital and conduction electron channels for an electron tunneling into a magnetic atom in a metallic host.  相似文献   

Shedding of Mycobacterium bovis in the nasal mucus of cattle infected experimentally with tuberculosis by the intranasal and intratracheal routes     

McCorry T  Whelan AO  Welsh MD  McNair J  Walton E  Bryson DG  Hewinson RG  Vordermeier HM  Pollock JM 《The Veterinary record》2005,157(20):613-618

Four groups of six calves were infected experimentally with either a low dose of approximately 10(4) colony-forming units (cfu) or a high dose of approximately 10(6) cfu of Mycobacterium bovis. Each dose was delivered by the intranasal and intratracheal routes. More severe disease was observed in the groups inoculated with the high dose. Visible lesions were identified in 21 of the 24 animals, all of which also gave positive skin tests and interferon-gamma (IFN-gamma) responses. Nasal shedding was detected in 15 of the 24 animals and the frequency of shedding was influenced by both the route and the dose of infection; no shedding was observed in the group infected intratracheally with the low dose. Two of the 15 confirmed shedders had no visible lesions at postmortem examination; both of these calves gave IFN-gamma responses but only one was skin test positive.  相似文献   

Efficacy of ivermectin against benzimidazole-resistant Nematodirus spathiger in sheep     

Whelan NC  Charleston WA  Alexander AM  Lees S 《New Zealand veterinary journal》1995,43(3):99-100

Eighteen helminth-free lambs were randomly allocated to six groups of three. Each lamb was dosed with 3300 infective larvae pooled from two isolates of Nematodirus spathiger known to be benzimidazole resistant. One lamb from each group was treated with oral ivermectin, one with oral oxfendazole and one left untreated 21 days after infection. All lambs were humanely killed 14 days later and small intestine worm counts performed. No Nematodirus were found in the ivermectin-treated lambs. Nematodirus numbers were reduced by 13% in oxfendazole-treated lambs relative to the control lambs.  相似文献   

Investigation of the effect of Equivac® HeV Hendra virus vaccination on Thoroughbred racing performance          下载免费PDF全文

K Schemann  EJ Annand  PA Reid  MF Lenz  PC Thomson  NK Dhand 《Australian veterinary journal》2018,96(4):132-141

Objective

To evaluate the effect of Equivac® HeV Hendra virus vaccine on Thoroughbred racing performance.

Design

Retrospective pre‐post intervention study.

Methods

Thoroughbreds with at least one start at one of six major south‐eastern Queensland race tracks between 1 July 2012 and 31 December 2016 and with starts in the 3‐month periods before and after Hendra virus vaccinations were identified. Piecewise linear mixed models compared the trends in ‘Timeform rating’ and ‘margin to winner’ before and after initial Hendra virus vaccination. Generalised linear mixed models similarly compared the odds of ‘winning’, ‘placing’ (1st–3rd) and ‘winning any prize money’. Timeform rating trends were also compared before and after the second and subsequent vaccinations.

Results

Analysis of data from 4208 race starts by 755 horses revealed no significant difference in performance in the 3 months before versus 3 months after initial Hendra vaccination for Timeform rating (P = 0.32), ‘Margin to winner’ (P = 0.45), prize money won (P = 0.25), wins (P = 0.64) or placings (P = 0.77). Further analysis for Timeform rating for 7844 race starts by 928 horses failed to identify any significant change in Timeform rating trends before versus after the second and subsequent vaccinations (P = 0.16) or any evidence of a cumulative effect for the number of vaccines received (P = 0.22).

Conclusion

No evidence of an effect of Hendra virus vaccination on racing performance was found. The findings allow owners, trainers, industry regulators and animal health authorities to make informed decisions about vaccination.  相似文献