Antioxidant supplementation,effect on post‐thaw spermatozoan function in three sturgeon species          下载免费PDF全文

P Li  MD Xi  H Du  XM Qiao  ZG Liu  QW Wei 《Reproduction in domestic animals》2018,53(2):287-295

High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post‐thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25‐0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post‐thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.  相似文献   

Dysphagia and esophageal dysfunction due to dystrophin deficient muscular dystrophy in a male Spanish water spaniel     

Brigitte B. McAtee  Johanna C. Heseltine  Ling T. Guo  Michael D. Willard  G. Diane Shelton 《The Veterinary quarterly》2018,38(1):28-32

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Transsphenoidal surgery using a high definition video telescope for pituitary adenomas in dogs with pituitary dependent hypercortisolism: Methods and results     

Adam N. Mamelak MD  FACS  Tina Jo Owen DVM  Diplomate ACVS  David Bruyette DVM  Diplomate ACVIM 《Veterinary surgery : VS》2014,43(4):369-379

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Effects of Sleep or Food Deprivation During Civilian Survival Training on Cognition, Blood Glucose and 3-OH-butyrate     

Lars Ståhle MD  PhD  Ewa Ljungdahl Ståhle PhD  Elisabeth Granström MD  PhD  Sven Isaksson PhD  Peter Annas PhD  Harry Sepp 《The Forestry Chronicle》2011,22(3):202-210

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Comparison of the bordered pits of two species of spruce (Pinaceae) in a green and kiln-dried condition and their effects on fluid flow in the stem wood in relation to wood preservation     

Usta  I; Hale  MD 《Forestry》2006,79(4):467-475

The relationship between bordered pit aspiration, pit sizesand permeability measured as preservative uptake and expressedas porosity was examined in two species of spruce, Sitka spruce(Picea sitchensis (Bong.) Carr.) grown in the UK and Easternspruce (Picea orientalis (L.) Link.) grown in Turkey, before(in the freshly felled green condition) and after kiln drying.A 2.5 per cent chromated copper arsenate preservative solutionwas allowed to flow in either the longitudinal or tangentialdirection of the stem wood and the uptake was assessed. Borderedpit anatomy was examined by light and scanning electron microscopyand image analysis was used to characterize the samples. Thepermeability of the wood declined following drying but lessso in the Sitka spruce. From an analysis of measurements madeon the wood features it appeared that basic density, latewoodpercentage and degree of pit aspiration were the most importantfeatures explaining these results. The structure of the borderedpits varied between the two species and the relative size ofthe aperture in comparison with the pit chambers was greaterin the Eastern spruce. The effects of these and other variablesincluding differences in conventional drying systems and naturaltree responses to environmental conditions on pit behaviouraffecting permeability are discussed.  相似文献   

A NATURAL CAT MUMMY     

Willard B 《Science (New York, N.Y.)》1930,71(1837):287-288

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Academy Forum: science and its critics     

Gaylin W  Gorovitz S 《Science (New York, N.Y.)》1975,188(4186):315

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A CENTURY OF GEOLOGIC SURVEYS IN PENNSYLVANIA     

Willard B 《Science (New York, N.Y.)》1936,84(2167):43-44

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THE PENNSYLVANIA ACADEMY OF SCIENCE     

Willard B 《Science (New York, N.Y.)》1935,81(2107):489-490

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Nitrogen uptake in riparian plant communities across a sharp ecological boundary of salmon density     

DD?Mathewson  MD?Hocking  TE?ReimchenEmail author 《BMC ecology》2003,3(1):4

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