Organohalogen residues in human adipose autopsy samples from six Ontario municipalities   总被引：3，自引：0，他引：3  

D T Williams  G L LeBel  E Junkins 《Journal of the Association of Official Analytical Chemists》1988,71(2):410-414

Human adipose tissue samples obtained during autopsies in 6 Canadian Great Lakes municipalities were analyzed for chlorobenzenes, polychlorobiphenyls, and organohalogen pesticide residues. The frequency of occurrence and the range and mean for 28 organohalogen residues are reported for male and female donors in each municipality. Overall mean residue values in females were significantly higher than those in males for hexachlorobenzene, beta-HCH, p,p'-DDE,p,p'-DDD + o,p'-DDT, and p,p'-DDT. The means and ranges of residue values were similar to those reported in previous Canadian surveys.  相似文献   

Elemental deposition downwind of a coal-fired power plant     

Lawrence E. Wangen  Michael D. Williams 《Water, air, and soil pollution》1978,10(1):33-44

Dry deposition of fly ash emitted by a coal-fired power plant has been calculated using a surface depletion Gaussian plume model. The subject plant is located in the southwestern United States. Soil samples collected downwind of this power plant have been chemically analyzed for selected trace elements (As, B, F, Hg, Se, Sr, U, and V) to determine concentration vs. distance trends. Gaussian plume deposition calculations predict very little increase of trace element concentrations in soils, except for those elements highly concentrated in fly ash emissions compared to soils. Trace element soil concentrations as a function of downwind distance generally confirm these predictions, with the possible exception of Se.  相似文献   

Extraction, processing, and storage effects on curcuminoids and oleoresin yields from Curcuma longa L. grown in Jamaica     

Green CE  Hibbert SL  Bailey-Shaw YA  Williams LA  Mitchell S  Garraway E 《Journal of agricultural and food chemistry》2008,56(10):3664-3670

Aromatic diarylheptanoid compounds from Curcuma longa Linn grown in Jamaica were quantified by UV-vis spectrophotometry and high-performance liquid chromatographic (HPLC) analyses. The oleoresin yields from ethanolic extracts were quantified and evaluated with regard to the effects of the type of postharvesting process and the type of extraction method conducted on the plant material. Fresh samples that were hot solvent extracted provided the highest oleoresin yields of 15.7% +/- 0.4 ( n = 3), and the lowest oleoresin yields of 7.8% +/- 0.2 ( n = 3) were from the dried milled samples that were cold solvent extracted. Data from the ASTA spectrophotometer assay confirmed that dried samples contained the highest curcuminoid content of 55.5% +/- 2.2 ( n = 6) at the fifth month of storage, and the fresh samples showed a curcuminoid content of 47.1% +/- 6.4 ( n = 6) at the third month of storage. A modified HPLC analysis was used to quantify curcumin content. Data from the HPLC analysis confirmed that the dried treated, hot extracted, room temperature stored samples had the highest curcumin content of 24.3%. A novel high-performance thin layer chromatography (HPTLC) method provided a chemical fingerprint of the C. longa with the use of a commercial curcumin standard.  相似文献   

New insights into the structural characteristics of the arabinogalactan-protein (AGP) fraction of gum arabic     

Mahendran T  Williams PA  Phillips GO  Al-Assaf S  Baldwin TC 《Journal of agricultural and food chemistry》2008,56(19):9269-9276

The structural characteristics of the gum exudate of Acacia senegal (gum arabic) have been investigated by monitoring the composition and physicochemical properties before and after treatment with proteolytic enzyme and various alkaline systems. Molecular mass ( M w) and radius of gyration ( R g) measurements were performed using gel permeation chromatography (GPC) coupled to refractive index, UV absorbance, and multiangle light scattering detectors and indicated that the macromolecules present have a compact structure. It was found that treatment with proteolytic enzyme caused the arabinogalactan-protein component (AGP) with average molecular mass approximately 2 x 10 (6) Da to degrade, yielding material of molecular mass approximately 4 x 10 (5) Da, whereas the bulk of the material corresponding to the protein-deficient arabinogalactan component (AG) with molecular mass 4 x 10 (5) remained unaffected. Barium hydroxide was found to hydrolyze the polysaccharide component (AG) itself in addition to the proteinaceous component as demonstrated in control experiments using dextran. However, sodium borohydride/sodium hydroxide treatments were unable to hydrolyze dextran and were assumed to hydrolyze only the proteinaceous component of gum arabic. The AGP component was completely degraded, yielding material of molecular mass approximately 4.5 x 10 (4) Da. It has been concluded, therefore, that the enzyme did not fully hydrolyze all of the protein present and that the AGP component of gum arabic consists of carbohydrate blocks of approximately 4.5 x 10 (4) Da linked to a polypeptide chain consistent with the wattle blossom structure. Because the AGP was degraded to differing extents using a mild and more severe sodium borohydride/sodium hydroxide treatment, it was concluded that the polysaccharide moieties were linked through both O-serine and O-hydroxyproline residues. The gum arabic sample was deglycosylated by treatment with anhydrous hydrogen fluoride and revealed the presence of two putative core proteins of approximately 3 x 10 (4) and approximately 5 x 10 (3) Da, respectively, which correspond to proteins of approximately 250 and 45 amino acids in length. A new model for the structure of the AGP component has been proposed.  相似文献   

Starving the soil of plant inputs for 50 years reduces abundance but not diversity of soil bacterial communities     

Penny R. Hirsch  Lucy M. Gilliam  Saran P. Sohi  Jennie K. Williams  Ian M. Clark  Phil J. Murray   《Soil biology & biochemistry》2009,41(9):2021-2024

If soil communities rely on plant-derived carbon, is biodiversity lost when this primary source is removed? Soil microbial and mesofaunal communities at the Rothamsted Highfield site were compared under a mixed grass sward, arable rotation and a section maintained as a bare-fallow for the past 50 years by regular tillage. Organic matter reserves have been degraded and microbial and mesofaunal numbers and mite diversity have declined in this unique bare-fallow site, where fresh carbon inputs have been drastically reduced. However, it supports a species-rich metabolically active bacterial community of similar diversity to that in soil maintained as grass sward. Thus in contrast to soil mesofauna, bacterial diversity (but not abundance) is apparently independent of plant inputs.  相似文献   

Nomenclature and genetic relationships of apples and pears from Terceira Island     

Nahla Bassil  Kim E. Hummer  Joseph D. Postman  Gennaro Fazio  Angela Baldo  Isabel Armas  Roger Williams 《Genetic Resources and Crop Evolution》2009,56(3):339-352

Heritage apple (Malus domestica Borkh. hybrids) and pear (Pyrus communis L. hybrid) trees grow in villages throughout Terceira Island, Azores, Portugal. Some of these pears have different names but similar morphology. The objective of this study was to determine synonymy, homology, and phylogeny of apples and pears from Terceira and to examine potential relationships of the island pears with standard apples and pears of Portuguese or American descent. Nine apple microsatellite markers were used to determine genetic relationships. Distance- and parsimony-based cluster analysis grouped these genotypes into separate apple and pear clades. The Terceira apples were divided into two clades: the maçā and the reineta-reinette. Among the 17 heritage apple genotypes, seven unique accessions were identified and four groups of synonyms, or possibly clones, were detected including: ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares; ‘Reineta Castanha’ and ‘Reineta Verde Miuda’; ‘Maçā Pêra,’ ‘Maçā Calhau’, ‘Pêro Branco’ from Salga and from Terra-Chā and ‘Maçā Marmelo’; and the five genotypes ‘Maçā Sao Joao’, ‘Malápio Rosa’, ‘Maçā Gaspar’, ‘Maçā Branca’ and ‘Maçā Pato’. In addition, two homonyms were detected. ‘Pêro Vermelho’ from Terra Chā was a separate genotype from a tree from Doze Ribeiras of the same name, but Pêro Branco from Terra Chā appears to be a clone that can be distinguished by an additional allele at CH1F07a from a tree with that name from Salga. One pair of apple clones, ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares appear to be derived from an unreduced gamete of ‘Golden Delicious.’ Another apple genotype ‘Maçā Acida’ could be a sibling of the ‘Maçā Pêra’ clonal group. Other tested standard apples from the US genebank were unrelated to Terceira genotypes. Of the seven heritage pears, five unique genotypes and one pair of synonyms were detected. ‘Pêra Papo Pintassilgo’ from Raminho and ‘Pêra Vermelha’ from the nursery of Serviço de Desenvolvimento Agario da Terceira (SDAT) were synonyms. ‘Passans du Portugal’ was related to ‘Pêra Cabaca’ but other standard pears from the US genebank were unrelated to Terceira genotypes. Future studies will include additional apple and pear cultivars from other Islands of the Azores and continental Portugal, and wild Asian species to further explore genetic relationships.  相似文献   

Growth and competitiveness of the New Zealand tree species Podocarpus cunninghamii is reduced by ex-agricultural AMF but enhanced by forest AMF     

Alwyn Williams  Hayley J. Ridgway 《Soil biology & biochemistry》2011,43(2):339-345

The composition of arbuscular mycorrhizal fungi (AMF) communities found in agricultural systems has been found to be very different to that of forest. The implications of this, if any, for the restoration of indigenous forest on ex-agricultural land is poorly understood. This study investigated the effect that AMF communities isolated from ex-agricultural and forest soils have on the growth of an indigenous New Zealand tree species (Podocarpus cunninghamii). The forest AMF community was isolated from a remnant stand of P. cunninghamii forest and the ex-agricultural AMF from a retired grazing grassland. In addition, the study examined how the two AMF communities affected the competitiveness of P. cunninghamii when grown in competition with an invasive grass species (Agrostis capillaris), which is frequently dominant on ex-agricultural land in New Zealand. P. cunninghamii growth was significantly decreased by inoculation with ex-agricultural AMF compared to forest AMF. Furthermore, the forest AMF community was able to significantly increase P. cunninghamii root production when in competition with A. capillaris. The findings suggest that when attempting to restore indigenous forest on ex-agricultural land, inoculation of tree seedlings with appropriate forest AMF may improve their growth and survival.  相似文献   

Land-use history has a stronger impact on soil microbial community composition than aboveground vegetation and soil properties     

Kamlesh Jangid  Mark A. Williams  Thomas M. Schmidt  William B. Whitman 《Soil biology & biochemistry》2011,43(10):2184-2193

The response of soil microbial communities following changes in land-use is governed by multiple factors. The objectives of this study were to investigate (i) whether soil microbial communities track the changes in aboveground vegetation during succession; and (ii) whether microbial communities return to their native state over time. Two successional gradients with different vegetation were studied at the W. K. Kellogg Biological Station, Michigan. The first gradient comprised a conventionally tilled cropland (CT), mid-succession forest (SF) abandoned from cultivation prior to 1951, and native deciduous forest (DF). The second gradient comprised the CT cropland, early-succession grassland (ES) restored in 1989, and long-term mowed grassland (MG). With succession, the total microbial PLFAs and soil microbial biomass C consistently increased in both gradients. While bacterial rRNA gene diversity remained unchanged, the abundance and composition of many bacterial phyla changed significantly. Moreover, microbial communities in the relatively pristine DF and MG soils were very similar despite major differences in soil properties and vegetation. After >50 years of succession, and despite different vegetation, microbial communities in SF were more similar to those in mature DF than in CT. In contrast, even after 17 years of succession, microbial communities in ES were more similar to CT than endpoint MG despite very different vegetation between CT and ES. This result suggested a lasting impact of cultivation history on the soil microbial community. With conversion of deciduous to conifer forest (CF), there was a significant change in multiple soil properties that correlated with changes in microbial biomass, rRNA gene diversity and community composition. In conclusion, history of land-use was a stronger determinant of the composition of microbial communities than vegetation and soil properties. Further, microbial communities in disturbed soils apparently return to their native state with time.  相似文献   

Hemoxygenase-2 is an oxygen sensor for a calcium-sensitive potassium channel     

Williams SE  Wootton P  Mason HS  Bould J  Iles DE  Riccardi D  Peers C  Kemp PJ 《Science (New York, N.Y.)》2004,306(5704):2093-2097

Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and in the carotid body it is crucial to respiratory control. However, the identity of the oxygen sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) is part of the BK channel complex and enhances channel activity in normoxia. Knockdown of HO-2 expression reduced channel activity, and carbon monoxide, a product of HO-2 activity, rescued this loss of function. Inhibition of BK channels by hypoxia was dependent on HO-2 expression and was augmented by HO-2 stimulation. Furthermore, carotid body cells demonstrated HO-2-dependent hypoxic BK channel inhibition, which indicates that HO-2 is an oxygen sensor that controls channel activity during oxygen deprivation.  相似文献   

Marine lava cave fauna: composition, biogeography, and origins     

Iliffe TM  Wilkens H  Parzefall J  Williams D 《Science (New York, N.Y.)》1984,225(4659):309-311

An assemblage of endemic cavernicolous marine invertebrates, including taxa found on both sides of the Atlantic Ocean of great phylogenetic age or with affinities to deep sea organisms, inhabits the Jameos del Agua cave, a sea waterflooded Holocene lava tube cave on Lanzarote in the Canary Islands. This marine cave contains both relicts from Tethyan times, such as an apparently new crustacean family belonging to what had been the monotypic class Remipedia, and relicts of groups that are now common only in the deep sea as well as species that occur outside the cave.  相似文献