Comments on the future of food supply veterinary medicine     

Wilson LL 《Journal of the American Veterinary Medical Association》2006,229(5):662; author reply 662-662; author reply 663

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Natural presence of Campylobacter spp. in various internal organs of commercial broiler breeder hens     

Cox NA  Richardson LJ  Buhr RJ  Fedorka-Cray PJ  Bailey JS  Wilson JL  Hiett KL 《Avian diseases》2006,50(3):450-453

Campylobacter are known to cause acute bacterial gastroenteritis in humans. Poultry products have been implicated as a significant source of these infections. Six experiments were performed to determine whether Campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. Broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. The birds were euthanatized, defeathered, and aseptically opened. To reduce the possibility of cross-contamination between samples, the thymus, spleen, and liver/gallbladder were aseptically removed prior to removal of the ceca. Individual samples were placed in sterile bags, packed on ice, and transported to the laboratory for evaluation. In this study Campylobacter were found in 11 of 43 thymii, eight of 43 spleens, four of 43 liver/gallbladders, and 30 of 43 ceca. Overall, 28 of 53 isolates from the above samples were Campylobacter coli and 25 of 53 isolates were found to be Campylobacter jejuni.  相似文献   

Surgical repair of traumatic oronasal fistula in a domestic short-haired cat     

Wilson N 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(12):1210-1213

A 9-month-old, spayed female domestic shorthair was presented with a suspected traumatic oronasal fistula. Two surgical attempts using mucosal advancement flaps and single layer closure failed, likely due to inadequate blood supply that was potentially exacerbated by excess tension and trauma on the graft.  相似文献   

Induction of GST and NQO1 in cultured bladder cells and in the urinary bladders of rats by an extract of broccoli (Brassica oleracea italica) sprouts   总被引：2，自引：0，他引：2  

Zhang Y  Munday R  Jobson HE  Munday CM  Lister C  Wilson P  Fahey JW  Mhawech-Fauceglia P 《Journal of agricultural and food chemistry》2006,54(25):9370-9376

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Influence of metoclopramide on gastroesophageal reflux in anesthetized dogs     

Wilson DV  Evans AT  Mauer WA 《American journal of veterinary research》2006,67(1):26-31

OBJECTIVE: To determine the effect of 2 doses of metoclopramide on the incidence of gastroesophageal reflux (GER) in anesthetized dogs. ANIMALS: 52 healthy dogs undergoing elective orthopedic surgery. PROCEDURE: In this prospective clinical study, dogs were evaluated before and during orthopedic surgery. The anesthetic protocol used was standardized to include administration of acepromazine, morphine, thiopental, and isoflurane. Dogs were randomly selected to receive an infusion of saline (0.9% NaCl) solution, a low dose of metoclopramide, or a high dose of metoclopramide before and during anesthesia. Treatment groups were similar with respect to age, body weight, duration of food withholding before surgery, duration of surgery, and dose of thiopental administered. Dogs were positioned in dorsal recumbency during surgery. A sensor-tipped catheter was inserted to measure esophageal pH during anesthesia. We defined GER as a decrease in esophageal pH to < 4 or an increase to > 7.5 that lasted more than 30 seconds. RESULTS: The high dose of metoclopramide (bolus loading dose of 1.0 mg/kg, IV, followed by continuous infusion at a rate of 1.0 mg/kg/h) was associated with a 54% reduction in relative risk of developing GER. The low dose did not significantly affect the incidence of GER. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of metoclopramide by bolus and constant rate infusion at doses much higher than commonly used will reduce the incidence but not totally prevent GER in anesthetized dogs undergoing orthopedic surgery.  相似文献   

Comparative analysis of cytokine gene expression in cerebrospinal fluid of horses without neurologic signs or with selected neurologic disorders     

Pusterla N  Wilson WD  Conrad PA  Mapes S  Leutenegger CM 《American journal of veterinary research》2006,67(8):1433-1437

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Data-loggers carried on a harness do not adversely affect sheep locomotion     

Hobbs-Chell H  King AJ  Sharratt H  Haddadi H  Rudiger SR  Hailes S  Morton AJ  Wilson AM 《Research in veterinary science》2012,93(1):549-552

The use of externally fitted motion sensors to animal subjects has the potential for allowing researchers to investigate subtle changes in animal movement that may occur with the onset of specific diseases. However, it is crucial to consider whether or not the use of such technology has an effect on the variables measured. Here, we examine the effect of a body harness data logging device on the locomotive patterns of female Merino sheep, Ovis aries. We extracted locomotion variables typical of motion sensor data (stride frequency, stride length, gait type, speed, and limb velocity) from high-definition video collected under controlled conditions. We found no significant difference between the variables measured in the harnessed and unharnessed conditions. Overall, our experiment demonstrates that data-loggers carried on a harness do not adversely affect sheep locomotion, and extended periods of habituation post-instalment of devices should ensure consistency and accuracy of data in future experiments.  相似文献   

Interferon-gamma and B-cell Activating Factor (BAFF) promote bovine B cell activation independent of TLR9 and T-cell signaling     

Buchanan R  Popowych Y  Dagenais C  Arsic N  Mutwiri GK  Potter AA  Babiuk LA  Griebel PJ  Wilson HL 《Veterinary immunology and immunopathology》2012,145(1-2):453-463

We previously reported that CD21(+) B cells purified from bovine blood do not respond to CpG-ODN stimulation unless either CD14(+) monocytes or B-cell Activating Factor (BAFF), a cytokine produced by activated monocytes, are present. In this report, we present evidence that CD14(+) monocytes are critical for CpG-specific lymphocyte proliferation within the peripheral blood mononuclear cell (PBMC) population but that this response is not mediated by soluble factors produced by CpG-activated monocytes. We further determine that bovine monocytes stimulated with IFN-γ induce expression of the BAFF gene and that recombinant IFN-γ and BAFF induced robust B cell activation when cultured in the absence of CpG ODN. These data suggest that CpG-stimulated monocytes may indirectly promote B cell activation by promoting release of cytokines and/or other soluble factors from accessory cells which in turn act on CpG-stimulated B cells to promote antigen-independent and T cell independent B cell activation. Understanding the T cell independent signals that induce B cell activation has important implications for understanding B cell development in locations where T cells are limited and in understanding polyclonal B cell activation that may contribute to autoimmune diseases.  相似文献   

Pharmacokinetics of lidocaine after intravenous administration to cows     

Cox S  Wilson J  Doherty T 《Journal of veterinary pharmacology and therapeutics》2012,35(3):305-308

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Tropical dry forest succession and the contribution of lianas to wood area index (WAI)     

G. Arturo Sánchez-Azofeifa  Margaret Kalácska  Mario Marcos do Espírito-Santo  G. Wilson Fernandes  Stefan Schnitzer 《Forest Ecology and Management》2009

The transmission and interception of light through the canopy is an important indicator of forest productivity in tropical forest ecosystems, and the amount of light that eventually reaches the forest floor is influenced by its interactions with leaves, branches, fruits, and flowers among many different canopy elements. While most studies of forest canopy light interception focus on leaf area index (LAI), very few studies have examined wood area index (WAI), which may account for a substantial component of light interception in tropical forests. The influence of lianas on the interception of light and their overall contribution to WAI is a potentially important factor, but it is generally overlooked because of its difficulty to assess. In this paper we evaluate the relative contribution that lianas have to the overall WAI and canopy openness as function of successional stage via a latitudinal comparison of sites across the Americas (Mexico, Costa Rica and Brazil). Our results suggest that lianas significantly increase WAI and decreases canopy openness. However, lianas were absent at all of our study sites where canopy openness exceeded 60%. Our data are the first to explicitly document the role of lianas in the estimation of WAI and, overall, they will contribute to better estimations of ecosystem level LAI in tropical environments, where there is a lack of data on WAI.  相似文献