Thiamine and its derivates in cattle blood measured by HPLC in healthy animals, in patients suffering from CCN and in their cohorts     

Höltershinken M  Höhling A  Witte B  Scholz H 《DTW. Deutsche tier?rztliche Wochenschrift》2007,114(6):212-218

The aim of this project was to develop a routine method to measure thiamine and its derivates in blood, to detect the need of vitamin B1-supply in cattle faster and more precise than until now using the transketolase test. For that, thiamine and its derivates (thiaminediphosphate = TDP,-monophosphate = TMP) were analysed in cattle blood by HPLC technique with post-column derivatization. Moreover, the levels in healthy cattle were compared with those in herds with single CCN cases. EDTA-blood-stored up to 10 days by 6 or 20 degrees C- was the most appropriate substrate. Sensitivitiy and specifity were significantly better than (TT, sum of all fractions): 1.95% and 2.10%; thiamine and TMP: 7.02% and 9.17%. The lowest concentration, which could be measured, was 0.5 ng/ml. 72 samples could be analysed daily. In the blood of 280 healthy calves and cows (group A), 201 clinically unsuspicious animals out of stocks with single cases of CCN (group B) and 12 patients with clinical obvious CCN (group C), thiamine and its phosphates were measured and the means calculated (ng/ml). After this, the thiamine status is characterized by (ng/ml): (A) thiamine 13.5 (4.61-28.8),TDP 51.0 (33.1-82.2), TMP 8.79 (2.23-18.1),TT 73.3 (44.6-114); (B) thiamine 8.73 (0.00-20.0), TDP 43.5 (15.5-75.7),TMP 6.15 (0.00-16.6), TT 59.4 (18.7-96.5); (C) thiamine 3.85 (0.00-1 1.5), TDP 14.5 (0.00-28.3), TMP 1.27 (0.00-4.87), TTP 19.6 (4.92-35.9). So, HPLC with post-column derivatization offers a precise, automatically working method for a fast diagnose of thiamine deficiency. The lowest critical value of TT is 40 ng/ml for healthy animals in the blood. The sample (EDTA-blood) can be posted simply at temperatures up to 20 degrees C. In comparison to other measuring methods, this HPLC-technique enables an early recognition of the risk to come down with CCN in endangered cattle herds.  相似文献   

What is your diagnosis? Swelling of the soft tissue around the carpal joint. Osteomyelitis of the accessory carpal bone     

Witte S  Rodgerson DH 《Journal of the American Veterinary Medical Association》2005,227(4):551-552

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Determination of vertebral range of motion using inertial measurement units in 27 Franches‐Montagnes stallions and comparison between conditions and with a mixed population          下载免费PDF全文

C. Heim  T. Pfau  V. Gerber  C. Schweizer  M. Doherr  G. Schüpbach‐Regula  S. Witte 《Equine veterinary journal》2016,48(4):509-516

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Fusarium head blight (FHB) in Flanders: population diversity, inter-species associations and DON contamination in commercial winter wheat varieties     

K. Audenaert  R. Van Broeck  B. Bekaert  F. De Witte  B. Heremans  K. Messens  M. Höfte  G. Haesaert 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(3):445-458

The presence of Fusarium spp. causing Fusarium head blight (FHB) of wheat was studied in Flanders (Belgium) in 2007 and 2008. Symptoms, deoxynivalenol content (DON), Fusarium spp. and trichothecene chemotypes were determined at seven locations on different commercial wheat varieties. Overall, significant differences in disease pressure between locations and varieties were observed within 1 year. In addition, we were able to detect consistent and significant resistance differences among the common varieties both under high disease pressure (2007) and low disease pressure (2008). The accumulation of DON was not related to the presence of F. graminearum but showed a clear correlation with rainfall during and after the period of anthesis. During the two-year survey, characterisation of 756 Fusarium samples by species-specific PCR designated F. poae and F. graminearum as the predominant species in Flanders. Furthermore, most of the ears were colonised by multiple FHB pathogens in 2007 whereas the Fusarium population was less complex in 2008. Log-linear analysis of these multiple (two- and three-way) species interactions revealed a clear correlation between F. poae and several pathogens of the FHB disease complex. Finally, chemotype analysis showed that F. culmorum and F. graminearum were respectively of the NIV chemotype and DON chemotype. 3-ADON and 15-ADON chemotypes occurred in more or less equal amounts within the F. graminearum population both in 2007 and 2008. The congruence of these results with observations throughout Europe are discussed.  相似文献   

Farm management factors associated with bulk tank somatic cell count in Irish dairy herds     

PT Kelly  K O'Sullivan  DP Berry  SJ More  WJ Meaney  EJ O'Callaghan  B O'Brien 《Irish veterinary journal》2009,62(Z4):S45

The relationship between bulk tank somatic cell count (SCC) and farm management and infrastructure was examined using data from 398 randomly selected, yet representative, Irish dairy farms where the basal diet is grazed grass. Median bulk tank SCC for the farms was 282,887 cells/ml ranging from 82,209 to 773,028 cells/ml. Two questionnaires were administered through face-to-face contact with each farmer. Herd-level factors associated with bulk tank SCC were determined using linear models with annual somatic cell score (i.e., arithmetic mean of the natural logarithm of bulk tank SCC) included as the dependent variable. All herd level factors were analysed individually in separate regression models, which included an adjustment for geographical location of the farm; a multiple regression model was subsequently developed. Management practices associated with low SCC included the use of dry cow therapy, participation in a milk recording scheme and the use of teat disinfection post-milking. There was an association between low SCC and an increased level of hygiene and frequency of cleaning of the holding yard, passageways and cubicles. Herd management factors associated with bulk tank SCC in Irish grazing herds are generally in agreement with most previous studies from confinement systems of milk production.  相似文献   

Theriogenology question of the month     

RM Radcliffe  TH Witte  SL Fubini  NG Ducharme  SH Cheong  RO Gilbert 《Journal of the American Veterinary Medical Association》2012,241(4):439-442

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The foot–surface interaction and its impact on musculoskeletal adaptation and injury risk in the horse          下载免费PDF全文

R. S. V. Parkes  T. H. Witte 《Equine veterinary journal》2015,47(5):519-525

The equine limb has evolved for efficient locomotion and high‐speed performance, with adaptations of bone, tendon and muscle. However, the system lacks the ability seen in some species to dynamically adapt to different circumstances. The mechanical interaction of the limb and the ground is influenced by internal and external factors including fore–hind mass distribution, lead limb, moving on a curve, shoeing and surface properties. It is unclear which of the components of limb loading have the largest effect on injury and performance but peak load, impact and vibration all play a role. Factors related to the foot–ground interface that limit performance are poorly understood. Peak performance varies vastly between disciplines but at high speeds such as racing and polo, force and grip are key limits to performance.  相似文献   

Bilateral leiomyoma in the testes of a horse          下载免费PDF全文

A. L. Stoll  R. Tucker  T. H. Witte  K. C. Smith 《Equine Veterinary Education》2015,27(3):113-115

A 19‐year‐old Hackney pony was referred to a veterinary teaching hospital for further investigation of left‐sided scrotal enlargement. Both testes were normally positioned within the scrotum and there was no pain on palpation. Ultrasonography revealed multiple masses of soft tissue echogenicity, located adjacent to the testicular parenchyma. They were hyperechoic when compared to the testicular parenchyma and affected both testes. Surgical excision was performed by closed castration. Gross examination revealed firm, white, nodular masses of varying size on the surface of both testes. The cut surface of individual masses had a dense, whorling, fibrous and somewhat shiny appearance. The histopathological diagnosis was bilateral, multicentric, well differentiated leiomyoma of the tunica albuginea. The smooth muscle origin was confirmed by immunohistochemical staining with an anti‐human monoclonal antibody reactive to smooth muscle actin. This is the first report of a bilateral, multicentric, leiomyoma in the testes of a horse confirmed by histopathology and immunohistochemistry. This case highlights that neoplasia should be considered as a differential for bilateral testicular enlargement.  相似文献   

Isolation and identification of molecular species of phosphatidylcholine and lysophosphatidylcholine from jojoba seed meal (Simmondsia chinensis)     

Léon F  Van Boven M  de Witte P  Busson R  Cokelaere M 《Journal of agricultural and food chemistry》2004,52(5):1207-1211

A mixture of lysophosphatidylcholine (LPC) and phosphatidylcholine (PC) has been isolated by column chromatography from a jojoba meal (Simmondsia chinensis) extract. The molecular species of both classes could be separated and isolated by C18 reversed phase HPLC. The two major compounds were identified by 1D and 2D (1)H and (13)C NMR, by MS, and by GC-MS as 1-oleoyl-3-lysophosphatidylcholine and 1,2-dioleoyl-3-phosphatidylcholine. Eight other molecular species of LPC and four other molecular species of PC could be assigned by comparison of the mass spectra of the isolated compounds with the spectra of the two major compounds. Complete characterization of the individual molecular species was achieved by GC and GC-MS analysis of the fatty acyl composition from the isolated compounds. The PC/LPC proportion in the phospholipid mixture from three different samples is 1.6 +/- 0.1. LPC is considered to be an important bioactive compound; the results of this study suggest further research for the evaluation of potential health benefits of jojoba meal phospholipids.  相似文献   

Multistep synthesis of a radiolabeled imaging probe using integrated microfluidics     

Lee CC  Sui G  Elizarov A  Shu CJ  Shin YS  Dooley AN  Huang J  Daridon A  Wyatt P  Stout D  Kolb HC  Witte ON  Satyamurthy N  Heath JR  Phelps ME  Quake SR  Tseng HR 《Science (New York, N.Y.)》2005,310(5755):1793-1796

Microreactor technology has shown potential for optimizing synthetic efficiency, particularly in preparing sensitive compounds. We achieved the synthesis of an [(18)F]fluoride-radiolabeled molecular imaging probe, 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), in an integrated microfluidic device. Five sequential processes-[18F]fluoride concentration, water evaporation, radiofluorination, solvent exchange, and hydrolytic deprotection-proceeded with high radio-chemical yield and purity and with shorter synthesis time relative to conventional automated synthesis. Multiple doses of [18F]FDG for positron emission tomography imaging studies in mice were prepared. These results, which constitute a proof of principle for automated multistep syntheses at the nanogram to microgram scale, could be generalized to a range of radiolabeled substrates.  相似文献