Development of buffer methods and evaluation of pedotransfer functions to estimate pH buffer capacity of highly weathered soils     

M. T. F. Wong  M. J. Webb  K. Wittwer 《Soil Use and Management》2013,29(1):30-38

The pH buffer capacity of a soil (pHBC) determines the amount of lime required to raise the pH of the soil layer from its initial acid condition to an optimal pH for plant growth and the time available under current net acid addition rate (NAAR) until the soil layer acidifies to a critical pH leading to likely production losses. Accurate values of pHBC can also be used to calculate NAAR from observed changes in soil pH. In spite of its importance, there is a critical shortage of pHBC data, likely due to the long period of time needed for its direct measurement. This work aimed to develop quick, simple and reliable methods of pHBC measurement and to test these methods against a slow (7‐day) titration used as benchmark. The method developed here calculates pHBC directly from the pH buffer capacity of the buffer solution and the increase in soil pH and corresponding decrease in pH of the buffer solution following mixing and equilibration. The pHBC values calculated using Adams and Evans or modified Woodruff buffers were in accord with those measured by slow titration. Buffer methods are easily deployed in commercial and research laboratories as well as in the field. The advantage of using buffer solutions to calculate pHBC instead of lime requirement is the broad application of this soil property. The pHBC of a soil is an intrinsic property that would not be expected to need remeasurement over periods of less than decades. Recurring lime requirement can be calculated from the soil's pHBC, initial and target pH values. A large proportion of the variability in pHBC was explained by the soil organic carbon content. This relationship between pHBC and soil organic carbon content allowed us to develop local pedotransfer functions to estimate pHBC for different regions of Australia.  相似文献   

INDUCED PARTHENOCARPY OF WATERMELON,CUCUMBER AND PEPPER     

Wong CY 《Science (New York, N.Y.)》1939,89(2314):417-418

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Age-related changes in mitogen-induced lymphocyte proliferation and polymorphonuclear neutrophil function in the piglet   总被引：5，自引：0，他引：5  

C D Hoskinson  B P Chew  T S Wong 《Journal of animal science》1990,68(8):2471-2478

Changes in concentration of plasma cortisol, mitogen-induced lymphocyte proliferation and the phagocytic and killing abilities of polymorphonuclear neutrophils (PMN) were assessed in 24 crossbred piglets at .5, 1, 3 and 6 wk of age. Concentrations of blood cortisol were high at birth but decreased (P less than .001) thereafter. Spontaneous lymphocyte proliferation was approximately 10-fold higher in the newborn than in pigs 6 wk old. The methods by which data on lymphocyte proliferation were expressed greatly influenced the results of the age-related changes in lymphocyte response. When lymphocyte proliferation was expressed as a stimulation index, there was an increase in mitogen-induced lymphocyte proliferation with advancing age. However, when lymphocyte proliferation was expressed as total counts per minute, mitogen-induced lymphocyte proliferation decreased with increasing age. In general, no significant age-related changes were observed in PMN function except for a transient decrease in PMN phagocytic ability at wk 3. Piglets were born with high concentrations of blood cortisol; this was associated with age-related changes in lymphocyte proliferation.  相似文献   

Sensitivity and specificity of the indirect-fluorescent antibody test and two enzyme-linked immunosorbent assays in canine dirofilariasis     

D Sisson  G Dilling  M M Wong  W P Thomas 《American journal of veterinary research》1985,46(7):1529-1533

Ninety dogs naturally infected with Dirofilaria immitis and 103 noninfected dogs, as determined by necropsy, were used to compare the sensitivity and specificity of the cuticular and somatic reactions of the indirect fluorescent antibody test (IFA-C and IFA-S, respectively) and 2 enzyme-linked immunosorbent assays (ELISA). In microfilaremic and amicrofilaremic heartworm-infected dogs, negative results were common for all serotests. In dogs without adult heartworms at necropsy, 32% to 49% were positive, using 1 ELISA, 27% to 29% were positive with the other ELISA, 15% to 36% were positive with the IFA-S, and 0% to 1% were positive using the IFA-C, depending on the classification of borderline reactions. The prevalence of false positive serotests was probably not due to the detection of precardial stages of D immitis in dogs obtained from areas of low endemicity. Until the causes of the false-positive tests are resolved, the use of currently available serotests for routine diagnostic screening or as criteria for instituting treatment is not recommended.  相似文献   

E_1级麻屑板用脲醛树脂胶的研究     

李兰亭  李晓平  翁向丽 《木材工业》1992,(3)

根据亚麻屑的胶接特性,从脲醛胶的固体含量、粘度、游离甲醛、耐水性、固化时间、施工胶液特性及经济效益等方面系统地研究了适合低甲醛释放量麻屑板生产用脲醛树脂胶的物理力学及化学性能。研究结果表明,使用改进的脲醛树脂胶(命名为DN—9号和DN—11号低毒腮醛胶)可以压制出甲醛释放量小于10mg/100g的E1级麻屑板,其物理力学性能达到GB 4896—4905—35标准  相似文献   

Erythrocyte and Biochemical Abnormalities as Diagnostic Markers in Dogs With Hemangiosarcoma Related Hemoabdomen          下载免费PDF全文

Richard W. Wong DVM  Mishka N. Gonsalves DVM  Michael L. Huber DVM  MS  Diplomate ACVS  Lon Rich DVM  PhD  Diplomate ACVP  Adam Strom DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2015,44(7):852-857

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Neutrophil Gelatinase–Associated Lipocalin in Dogs with Naturally Occurring Renal Diseases     

W.‐L. Hsu  Y.‐S. Lin  Y.‐Y. Hu  M.‐L. Wong  F.‐Y. Lin  Y.‐J. Lee 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(2):437-442

Background

Neutrophil gelatinase–associated lipocalin (NGAL) is released from renal tubular cells after injury and serves in humans as a real‐time indicator of active kidney damage, including acute kidney injury (AKI) and chronic kidney disease (CKD). However, NGAL concentrations in dogs with naturally occurring AKI or CKD rarely have been explored in detail.

Hypothesis/Objectives

The goal of this study was to evaluate whether NGAL can serve as a useful biomarker in dogs with naturally occurring renal disease.

Animals

Client‐owned dogs with renal disease (57) and control dogs without any disease (12) were examined.

Methods

Serum NGAL (sNGAL) and urine NGAL (uNGAL) concentrations were measured in each animal by a newly developed ELISA system. Demographic, hematologic, and serum biochemical data were recorded. Survival attributable to AKI and CKD was evaluated at 30 days and 90 days, respectively.

Results

Serum and urine NGAL concentrations in azotemic dogs were significantly higher than in nonazotemic dogs and were highly correlated with serum creatinine concentration (P < .05). Among CKD dogs, death was associated with significantly higher sNGAL and uNGAL concentrations compared with survivors. Receiver‐operating characteristic curve (ROC) analysis showed that sNGAL was better than serum creatinine concentration when predicting clinical outcomes for CKD dogs (P < .05). The best cutoff point for sNGAL was 50.6 ng/mL, which gave a sensitivity and a specificity of 76.9 and 100%, respectively. Furthermore, dogs that had higher concentrations of sNGAL survived for a significantly shorter time.

Conclusion

sNGAL is a useful prognostic marker when evaluating dogs with CKD.  相似文献   

Effect of hypoxia on generation of neurospheres from adipose tissue-derived canine mesenchymal stromal cells     

D.J. Chung  A. Wong  K. Hayashi  C.E. Yellowley 《Veterinary journal (London, England : 1997)》2014,199(1):123-130

Adipose tissue-derived mesenchymal stromal cells (AT-MSCs) are good candidates for cell therapy due to the accessibility of fat tissue and the abundance of AT-MSCs therein. Neurospheres are free-floating spherical condensations of cells with neural stem/progenitor cell (NSPC) characteristics that can be derived from AT-MSCs. The aims of this study were to examine the influence of oxygen (O₂) tension on generation of neurospheres from canine AT-MSCs (AT-cMSCs) and to develop a hypoxic cell culture system to enhance the survival and therapeutic benefit of generated neurospheres.AT-cMSCs were cultured under varying oxygen tensions (1%, 5% and 21%) in a neurosphere culture system. Neurosphere number and area were evaluated and NSPC markers were quantified using real-time quantitative PCR (qPCR). Effects of oxygen on neurosphere expression of hypoxia inducible factor 1, α subunit (HIF1A) and its target genes, erythropoietin receptor (EPOR), chemokine (C-X-C motif) receptor 4 (CXCR4) and vascular endothelial growth factor (VEGF), were quantified by qPCR. Neural differentiation potential was evaluated in 21% O₂ by cell morphology and qPCR.Neurospheres were successfully generated from AT-cMSCs at all O₂ tensions. Expression of nestin mRNA (NES) was significantly increased after neurosphere culture and was significantly higher in 1% O₂ compared to 5% and 21% O₂. Neurospheres cultured in 1% O₂ had significantly increased levels of VEGF and EPOR. There was a significant increase in CXCR4 expression in neurospheres generated at all O₂ tensions. Neurosphere culture under hypoxia had no negative effect on subsequent neural differentiation. This study suggests that generation of neurospheres under hypoxia could be beneficial when considering these cells for neurological cell therapies.  相似文献   

Performance of cotton single yarns and knitted fabrics produced by a 2-step spinning method     

Jie Feng  Bingang Xu  Xiaoming Tao  Cheukwun Wong 《Fibers and Polymers》2014,15(4):882-890

A low torque spinning method has been recently developed by integrating the ring spinning process with false twisting techniques. As a result of false twist and true twist, a novel single yarn of low torque and soft handle is generated. Based on the similar spinning principle, this paper proposed a 2-step spinning method, which is composed of twisting-anduntwisting operation and can be considered as a variant of the low torque spinning method. The proposed spinning method separates the false twisting process into twisting and untwisting processes which are combined in one step in the low torque spinning method. In this paper, physical properties of yarns produced by the proposed spinning method were evaluated and compared with the conventional yarns. Influences of various spinning parameters on the properties of 2-step yarns were also examined. Furthermore, a systematic study of the physical properties of the 2-step yarns and resultant fabrics with respect to the conventional and low torque ones was carried out. The experimental results showed that the 2-step yarns exhibit slightly higher tenacity, lower wet snarl and much less hairiness than the conventional yarns, which, however, are inferior to the low torque yarns, particularly the evenness. The fabrics made by the 2-step yarns show the softest handle feeling, best airpermeability and moderate spirality angle with acceptable pilling resistance.  相似文献   

Modification of Marine Natural Product Ningalin B and SAR Study Lead to Potent P-Glycoprotein Inhibitors     

Chao Yang  Iris L. K. Wong  Wen Bin Jin  Tao Jiang  Larry M. C. Chow  Sheng Biao Wan 《Marine drugs》2014,12(10):5209-5221

In this study, new marine ningalin B analogues containing a piperazine or a benzoloxy group at ring C have been synthesized and evaluated on their P-gp modulating activity in human breast cancer and leukemia cell lines. Their structure-activity relationship was preliminarily studied. Compounds 19 and 20 are potent P-gp inhibitors. These two synthetic analogues of permethyl ningalin B may be potentially used as effective modulators of P-gp-mediated drug resistance in cancer cells.  相似文献