What is your diagnosis? Ascites fluid from an 11‐year‐old dog with epigastric bulging     

Jae‐Ik Han  Hye‐Jin Jang  Dong‐Woo Chang  Gon‐Hyung Kim  Byeongwoo Ahn  Ki‐Jeong Na 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(4):541-544

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Low dietary inorganic phosphate affects the lung growth of developing mice     

Cheng-Xiong Xu  Hua Jin  Youn-Sun Chung  Ji-Young Shin  Soon-Kyung Hwang  Jung-Taek Kwon  Sung-Jin Park  Eun-Sun Lee  Arash Minai-Tehrani  Seung-Hee Chang  Min-Ah Woo  Mi-Suk Noh  Gil-Hwan An  Kee-Ho Lee  Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):105-113

Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.  相似文献   

Dilation of the olfactory bulb cavity concurrent with hydrocephalus in four small breed dogs     

Jung-Hyun Kim  Hyo-Won Jeon  Eung-Je Woo  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):173-175

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Protein digestibility of porcine colostrum by neonatal pigs     

Carol Lin  D.C. Mahan  Guoyao Wu  Sung Woo Kim   《Livestock Science》2009,121(2-3):182-186

Colostrum plays an important role in neonatal growth and development. However, little is known about the digestion of macronutrients in colostrum of any species. This study was conducted with the neonatal piglet model to determine the digestibility of proteins in porcine colostrum. Twelve, 1-d-old, male piglets were selected from 3 litters (4 pigs/litter) and housed individually in metabolism crates with heating lamps to maintain a temperature of 35 °C. Colostrum (13 L) was collected from 400 sows (30 to 40 mL/sow) within 12 h postpartum after injection of oxytocin. All piglets were fed colostrum containing 0.25% (DM basis) chromium oxide as an external marker based on the following feeding program: 6 meals/d for an entire 3-d period; with 40 mL/meal for d 1 (240 mL/d), 55 mL/meal for d 2 (330 mL/d), and 70 mL/meal for d 3 (420 mL/d). Colostrum was hand-fed using baby milk bottles. Entire fecal samples with the chromium green color were collected each day after colostrum feeding. Fecal collection was terminated before the fading of the green color. Fecal samples were weighed (10.3 ± 1.0 g/pig), stored in − 20 °C, freeze-dried, and thoroughly ground for chemical analysis. Blood samples were collected at 0900 h of d 3 to obtain plasma samples for amino acid and immunoglobulin (Ig) G analysis. Digestibilities of crude protein and DM in colostrum, defined as the percentage of ingested colostral crude proteins and DM that disappeared in the gut, averaged 96.9 ± 0.4% and 98.3 ± 0.2%, respectively. Digestibility of total amino acids (protein-bound plus free amino acids) in the colostrum was 98.3 ± 0.1%, with the values being 98.5 ± 0.3, 98.2 ± 0.4, and 98.3 ± 0.3%, respectively, for Lys, Thr, and Arg. Plasma and colostral IgG content were 3.4 ± 0.3 and 3.8 ± 0.7 g/L, respectively. In conclusion, protein-bound and free amino acids in porcine colostrum were highly digestible and available to neonatal pigs.  相似文献   

Evaluation of novel adjuvant Eimeria profilin complex on intestinal host immune responses against live E. acervulina challenge infection     

Lee SH  Lillehoj HS  Jang SI  Lee KW  Kim DK  Lillehoj EP  Yancey RJ  Dominowski PJ 《Avian diseases》2012,56(2):402-405

The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN [T]) (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 x 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1+/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks.  相似文献   

The immunostimulatory activity and stability of grass carp (Ctenopharyngodon idellus) roe lectin     

Ng TB  Lam YW  Woo NY 《Veterinary immunology and immunopathology》2003,94(3-4):105-112

A hexameric rhamnose-specific lectin with a molecular mass of 205kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10 micro g/ml on the phagocytic activity of seabream (Sparus sarba) macrophages. It was unstable toward heat (temperature > or =40 degrees C), acid (0.1M HCl), alkali (0.1M NaOH), trypsin and succinylation.  相似文献   

Cytology of Cork Layer Formation of Citrus and Limited Growth of Elsinoe fawcettii in Scab Lesions     

Ki Woo Kim  Jae-Wook Hyun  Eun Woo Park 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(2):129-138

Ultrastructural aspects of host–parasite interactions were investigated in fruits and leaves of citrus (satsuma mandarin) infected with Elsinoe fawcettii. Fungal infection induced host tissues to form cork layers bordering the necrotic areas below the infected sites. The cork layers were composed of compact host cells with convoluted cell walls and alternating lamellations, indicating ligno–suberized tissues in the wound periderm. No host tissues below the cork layers were invaded by hyphae. Hyphae grew intercellularly and intracellularly, often causing hypertrophy and compartmentalization of infected host cells. Also, host cells adjacent to invading hyphae showed accumulation of electron-dense materials and the formation of host cell wall protuberances in intercellular spaces. Hyphae had concentric bodies that showed an electron-transparent core surrounded by an electron-dense layer with radiating filamentous structures on their surface. One or more intrahyphal hyphae were found in the cytoplasm of intercellular or intracellular hyphae. These results suggest that the ligno–suberized cork layers in the wound periderm of citrus act as a protective barrier, which leads to restricted growth of E. fawcettii in bordered scab lesions. The fungus is thought to form concentric bodies and intrahyphal hyphae as a survival mechanism against the water- and nutrient-deficient environments that occur in the cork layers of necrotic host parts.  相似文献   

Cardioversion with lidocaine of vagally associated atrial fibrillation in two dogs     

N. Sydney Moïse DVM  Dipl. ACVIM  Romain Pariaut DMV  Dipl. ACVIM  Anna R.M. Gelzer Dr. med. vet.  Dipl. ACVIM  Marc S. Kraus DVM  Dipl. ACVIM  Seung Woo Jung DVM 《Journal of Veterinary Cardiology》2005,7(2):143

Two dogs with acute onset atrial fibrillation (AF) were cardioverted to sinus rhythm by the administration of 2 mg/kg lidocaine given intravenously. Each dog was believed to have AF initiated because of elevated vagal tone. This report has potential clinical impact for a subset of dogs because it offers a treatment to circumvent persistent AF. Furthermore, this encouraging result of a pharmacologic cardioversion suggests that further investigation would be of interest to ascertain the effectiveness and mechanism of the antiarrhythmic action of lidocaine in vagally induced AF.  相似文献   

Systematic Numbering of Vegetative Compatibility Groups in the Plant Pathogenic Fungus Fusarium oxysporum     

Kistler HC  Alabouvette C  Baayen RP  Bentley S  Brayford D  Coddington A  Correll J  Daboussi MJ  Elias K  Fernandez D  Gordon TR  Katan T  Kim HG  Leslie JF  Martyn RD  Migheli Q  Moore NY  O'Donnell K  Ploetz RC  Rutherford MA  Summerell B  Waalwijk C  Woo S 《Phytopathology》1998,88(1):30-32

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Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells   总被引：1，自引：0，他引：1  

Hyun-Ji Kim  Mi-Hyoung Kim  Yun-Young Byon  Jae Woo Park  Youngheun Jee  Hong-Gu Joo 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):39-44

An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell viability. Using 2 different assays, a pretreatment with APG significantly increased the viability of BMs against gamma radiation. APG-treated BMs had a significantly higher amount of IL-12, which is a major cytokine for immune responses, compared with the medium-treated BMs. The expression of MHC class II molecules of APG-treated BMs was also increased, and APG-treated BMs showed significantly higher levels of allogeneic CD4⁺ T lymphocyte proliferation. Furthermore, APG-treated mice had a larger number of BMs after gamma radiation than the control mice, and the BMs of APG-treated mice were successfully cultured into dendritic cells, which are the representative antigen-presenting cells. Overall, this study shows that APG alters the phenotype of BMs, increases the viability and alloreactivity of BMs after gamma radiation both in vitro and in vivo. Therefore, APG may be a good candidate radioprotective agent for BMs.  相似文献