Protein Composition of Seminal Plasma in Fractionated Stallion Ejaculates     

AM Kareskoski  MM Rivera del Alamo  K Güvenc  T Reilas  JJ Calvete  H Rodriguez‐Martinez  M Andersson  T Katila 《Reproduction in domestic animals》2011,46(1):e79-e84

Seminal plasma (SP) contains several types of compounds derived from the epididymides and accessory glands. The aim of this study was to examine the protein composition of different ejaculate fractions. Trial I: fractionated ejaculates were collected from two normal and two subfertile stallions. Samples containing pre‐sperm fluid and the first sperm‐rich jets (HIGH‐1), the main sperm‐rich portion (HIGH‐2), the jets with low sperm concentrations (LOW), and a combined whole‐ejaculate (WE) sample was centrifuged, and the SP was filtered and frozen. A part of each SP sample was stored (5°C, 24 h) with spermatozoa from HIGH‐2 and skim milk extender. Sperm motility was evaluated after storage in extender mixed with the stallion’s own SP or SP from one of the other stallions (sperm from a normal stallion stored in SP from a subfertile stallion and vice versa). Protein composition was analysed using reverse‐phase liquid chromatography (RP‐HPLC), N‐terminal sequencing and mass spectrometry. The area‐under‐the‐curve (AUC) was used for quantitative comparison of proteins within fractions. Trial II: semen samples were collected from seven stallions. Fractions with the highest (HIGH) and lowest (LOW) sperm concentrations and WE samples were examined using SDS‐PAGE and densitometry. No significant differences emerged between fractions in the AUC‐values of the Horse Seminal Protein‐1 (HSP‐1) and HSP‐2 peaks, or the peak containing HSP‐3 and HSP‐4 (HSP‐3/4). Levels of HSP‐1, HSP‐2 and HSP‐3/4 were not significantly correlated with total sperm motility, progressive sperm motility or average path velocity after storage. Significant differences between ejaculate fractions in the amount of different protein groups present in SP were not found in Trial I; but in Trial II, the proteins in the 60–70 kDa range were more abundant in LOW than in HIGH and WE, indicating that this band contained proteins derived mainly from the seminal vesicles, which produce most of the SP in LOW.  相似文献   

Pathology and immunohistochemical findings of west nile virus infection in psittaciformes     

Palmieri C  Franca M  Uzal F  Anderson M  Barr B  Woods L  Moore J  Woolcock P  Shivaprasad HL 《Veterinary pathology》2011,48(5):975-984

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Tick paralysis in dogs and cats in Australia: treatment and prevention deliverables from 100 years of research     

AM Padula  EM Leister  RA Webster 《Australian veterinary journal》2020,98(1-2):53-59

This review of tick paralysis caused by Ixodes holocyclus in Australia addresses the question: What are the key discoveries that have enabled effective treatment and prevention of tick paralysis in dogs and cats? Critical examination of 100 years of literature reveals that arguably only three achievements have advanced treatment and prevention of tick paralysis in animals. First, the most significant treatment advance was the commercial availability of tick antiserum in the 1930s. Hyperimmune serum currently remains the only specific anti-paralysis tick therapy available to veterinarians in Australia. Second, advances in veterinary critical care have increased survival rates of the most severely affected dogs and cats. Critical care advancements have been enabled through specialised veterinary hospitals that can provide appropriate care 24 h a day, and advanced training of veterinarians, veterinary nurses and technicians. Third, perhaps that biggest advance of all in the last 100 years of research has been the commercial availability of the isooxazoline class of acaricidal preventatives in Australia specifically for I. holocyclus. This highly effective class of preventatives offers long duration of action, low cost, spot-on or oral formulations and a low rate of adverse reactions. Animal owners and veterinarians now have the most useful tool of all – a reliable preventative. This review reveals the key events in research over the last 100 years and the tortuous pathway to delivering better treatment and preventative options for this enigmatic Australian parasite.  相似文献   

Red-bellied black snake (Pseudechis porphyriacus) envenomation in 17 dogs: clinical signs,coagulation changes,haematological abnormalities,venom antigen levels and outcomes following treatment with a tiger-brown snake antivenom     

ER Finney  AM Padula  EM Leister 《Australian veterinary journal》2020,98(7):319-325

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SnakeMap: four years of experience with a national small animal snake envenomation registry     

M Boller  K Kelers  MA Stevenson  KD Winkel  S Hardjo  J Heller  PR Judge  HM Ong  AM Padula  C Reddrop  LCP Santos  CR Sharp  L Smart  KL Swindells  D Tabrett  JR Wierenga 《Australian veterinary journal》2020,98(9):442-448

SnakeMap is a national cloud-based, veterinary snakebite registry. It was designed to prospectively collect data of the clinical circumstances and temporospatial information on cases of snake envenomation in dogs and cats. We herein introduce the project and summarise the data from the first 4 years of SnakeMap. The registry is a veterinary community-based online database allowing case entry from veterinary hospitals across Australia. Registry data comprise hospital characteristics, patient characteristics, envenoming snake type, treatment and outcome variables, including time and geolocation of the snake bite. We present summative information on select key variables from the SnakeMap registry (1 July 2015 to 30 June 2019). Twenty-eight hospitals from 6 states/territories entered 624 cases into the registry, including 419 dogs (67%) and 205 cats (33%). Bite time was available in 216 animals of which 90 (42%) were reported to be bitten in the 3 hours between 03:00 pm and 05:59 pm; median bite to presentation interval was 60 (interquartile range [IQR] 30, 211) minutes in dogs and 95 (IQR 41, 238) minutes in cats. Bites occurred in the owner's yard in 356 dogs (85%) and 53 cats (26%). A snake venom detection kit was used in 172 cases (28%) and antivenom was administered in 523 cases (85%). Most animals (n = 534, 88%) survived to discharge (median hospitalisation of 25 [IQR 16, 62] hours). SnakeMap effectively collects relevant clinical data from dogs and cats with presumed snake bite and provides locally specific information on the epidemiology of snake envenomation in small animals.  相似文献   

USE OF ^99mTc-MERCAPTOACETYLTRIGLYCINE TO EVALUATE RENAL FUNCTION IN HORSES     

Philip R.  Woods  DVM  PhD  Wm. Tod  Drost  DVM  Cyril R.  Clarke  BVSc  PhD  Carolyn J.  Rodebush  RT 《Veterinary radiology & ultrasound》2000,41(1):85-88

Ten healthy horses were injected intravenously with 99mTc-MAG3 and the disappearance of radioactivity from the blood was measured. The total body clearance (Cl(B)) and elimination half-life (t1/2(beta)) were 7.9 +/- 1.5 ml/kg/minute and 32.8 +/- 4.1 minutes, respectively. The disappearance of 99mTc-MAG3 from the blood of 2 horses with compromised renal function was also measured. The data suggest that 99mTc-MAG3 is a useful and clinically applicable radiopharmaceutical for measurement of effective renal blood flow in the horse.  相似文献   

Improving initial survival in cultured seahorses, Hippocampus abdominalis Leeson, 1827 (Teleostei: Syngnathidae)     

Chris M. C. Woods 《Aquaculture (Amsterdam, Netherlands)》2000,190(3-4):377-388

This investigation aimed at improving early juvenile survival in cultured seahorses, Hippocampus abdominalis. Phototaxis from birth until 2 months of age was tested and juveniles were found to be positively phototactic within this age range. A comparison of the effect of culture vessel background (white vs. black vs. clear jars) on feeding efficiency at 1 week and 1 month of age using Artemia as prey revealed an age-dependent effect upon both attack rate and capture success. At 1 week, both attack rate and capture success were higher for juveniles contained in clear jars, and capture success was higher in black jars, as compared with white jars. At 1 month, there was no significant difference in attack rate between different jars, although capture success was higher in clear and white jars than in black jars. In a top-illuminated glass aquarium, juveniles with access to the water surface had lower survival (mean±1 SE=50±4.5%) than those without access to the water surface (73.3±4.2%). This was due to the ingestion of air bubbles when preying on Artemia nauplii congregating near the water surface. For side-illumination there was no such difference. Glass aquaria that were side-illuminated and blacked-off upwards from the waterline supported high juvenile survival (80.4±4.0% per brood) from birth through to 2 months of age in four broods.  相似文献   

QTL analysis of resistance to powdery mildew in hop (<Emphasis Type="Italic">Humulus lupulus</Emphasis> L.)     

J. A. Henning  D. H. Gent  M. S. Townsend  J. L. Woods  S. T. Hill  D. Hendrix 《Euphytica》2017,213(4):98

Hop with powdery mildew [HPM: caused by Podosphaera macularis (Wallr.) U. Braun & S. Takam.] results in significant losses in hop production by reducing yield and quality. One means of increasing yield and quality is the production of resistant hop lines. Breeding for resistance can be significantly improved and accelerated by use of marker-assisted selection. The objective of this preliminary study was to identify QTLs and markers for genetic resistance to HPM. A bi-parental mapping population between the resistant line “Newport” and susceptible line ‘21110M’. Phenotypic data was scored under controlled greenhouse conditions. Significant differences among offspring were observed and disease resistance did not follow a distinct binomial distribution, suggesting quantitative genetic control. Genotyping-by-sequencing resulted in approximately 375 K SNP markers, which were filtered down to 2263 markers mapped to 10 linkage groups. Interval Mapping identified four QTLs with one on linkage group 1 and three located on linkage group 6. Composite interval mapping identified three QTLs, all located on linkage group 6. Mixed linear models identified 15 markers associated with expression of resistance to HPM. Three of these 15 SNPs were also identified in QTL-CIM analysis. Evaluation of the scaffolds containing the significant SNP markers identified seven putative genes—several of which appear involved in disease resistance in other plant species. The SNP markers identified in this study still require validation in unrelated populations prior to implementation in breeding programs.  相似文献   

Effects of nutrition, sex of calf and breed type on response to zeranol: preweaning growth   总被引：1，自引：0，他引：1  

B L Woods  N W Bradley  K K Schillo  S R Lowry 《Journal of animal science》1990,68(4):919-922

An experiment was conducted to evaluate the effects of breed, sex and plane of nutrition on the growth response to zeranol in Angus and crossbred calves prior to weaning. Eighty-eight heifers and 118 steers received either a high or low plane of nutrition using a first and last grazing technique. Half of the calves in each nutrition group received a zeranol implant (36 mg) at an average age of 3.4 mo. Both zeranol and the higher level of nutrition increased (P less than .001) growth rate prior to weaning (7.4 mo of age). Zeranol did not affect hip height at weaning (P greater than .1), but calves on the higher plane of nutrition were taller (P less than .01) than calves on the lower plane of nutrition. The zeranol x nutrition interaction was not significant (P greater than .1) for growth rate or hip height. Steers grew faster (P less than .01) preweaning and were taller (P less than .01) at weaning than heifers. Crossbred calves gained more rapidly (P less than .001) preweaning and were taller (P less than .001) at weaning than Angus calves were. Neither sex nor breed interacted with zeranol to influence any of the traits examined. Based on these results we conclude that preweaning growth was affected by zeranol and this effect was consistent across sexes, breeds and planes of nutrition tested.  相似文献   

Changes in accessory sex glands of stallions after sexual preparation and ejaculation     

J A Weber  R T Geary  G L Woods 《Journal of the American Veterinary Medical Association》1990,196(7):1084-1089

Ultrasonographic images of the accessory sex glands of 8 stallions were recorded immediately prior to sexual preparation, immediately after sexual preparation, and immediately after ejaculation. Relative size changes, determined by measurements of ultrasonograms of accessory sex gland, were contrasted. Length and width of the bulbourethral glands increased significantly (P less than 0.05) after sexual preparation and decreased significantly (P less than 0.05) after ejaculation. The increase in bulbourethral gland volume following sexual preparation was correlated significantly (P less than 0.01) with the number of false mounts attempted by stallions during sexual preparation. Diameter of the pelvic portion of the urethra did not change significantly (P greater than 0.05) after sexual preparation or after ejaculation. Prostate gland lobular and isthmic thickness increased significantly (P less than 0.05) after sexual preparation and decreased significantly (P less than 0.05) after ejaculation. Total and lumenal diameter of the ampullae increased significantly (P less than 0.05) after sexual preparation and decreased significantly (P less than 0.05) after ejaculation. Diameter of the ampulla wall did not change significantly (P greater than 0.05) after sexual preparation or ejaculation. Reduction of the lumenal area of the ampullae after ejaculation was not correlated (P greater than 0.05) to concentration of spermatozoa or total spermatozoa per ejaculate. Total and lumenal diameters of the vesicular glands increased significantly (P less than 0.05) after sexual preparation and decreased significantly (P less than 0.05) after ejaculation. Diameter of the vesicular gland wall did not change significantly (P greater than 0.05) after sexual preparation or ejaculation.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献