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排序方式: 共有1346条查询结果,搜索用时 484 毫秒
81.
82.
A diagnosis of dicoumarol toxicity in a herd of Friesian cattle was made following investigation of the deaths of three mature cows and eleven yearling heifers. Affected stock had been fed wrapped, bailed silage containing approximately 90% sweet vernal grass ( Anthoxanthum odoratum ). Sweet vernal grass contains coumarin, which can be converted to dicoumarol, a vitamin K antagonist, through the action of moulds. Most deaths were preceded by lethargy, severe anaemia and subcutaneous and internal haemorrhage. Dicoumarol toxicosis was suspected based on clinical signs, necropsy findings and prolonged prothrombin and activated partial thromboplastin times. Dicoumarol analysis of blood from affected animals and silage confirmed the diagnosis. 相似文献
83.
A total of 31 isolates of Haemophilus parasuis obtained from Australian pigs were serotyped by the Kielstein-Rapp-Gabrielson scheme. The isolates were assigned to serovar 1 (1 isolate), serovar 2 (1 isolate), serovar 4 (4 isolates), serovar 5 (7 isolates), serovar 9 (2 isolates), serovar 10/7 (4 isolates), serovar 12 (1 isolate) and serovar 13 (6 isolates). The remaining 5 isolates could not be assigned to a serovar. Two different serovars (5 and 13) were detected in one herd. The only 2 isolates obtained from clinically normal pigs (from the same herd) were serovar 9. The common serovars were isolated from pigs with pneumonia as well as from pigs with conditions of the Glässer's disease type. The serological heterogeneity amongst Australian isolates of H parasuis has important implications for the use of vaccines to control Glässer's disease. 相似文献
84.
M E Tocidlowski C L Merrill M R Loomis J F Wright 《Journal of zoo and wildlife medicine》2001,32(2):257-259
The clinical, gross necropsy, and histopathology findings in two unrelated desert grassland whiptail lizards (Cnemidophorus uniparens) with teratoma are described. The desert grassland whiptail is a parthenogenic lizard species with a polyploid chromosomal complement. The chromosome composition of the teratomas from these lizards was not determined. 相似文献
85.
M. Hill K. Launis C. Bowman K. McPherson J. Dawson J. Watkins M. Koziel M. S. Wright 《Euphytica》1955,85(1-3):119-123
Summary A synthetic Bt gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis was successfully introduced into elite maize using microprojectile bombardment of immature embryos. The method used to initiate and identify transformation events is described. We describe the detailed parameters used for the Biolistics device as well as the plasmids used for the transformations. The plasmids contained the synthetic Bt gene driven by either the 35S CaMV promoter or a combination of two tissue-specific promoters, leaf and pollen, derived from maize. Specific conditions for the culture of Type I callus from immature embryos, the phosphinothricin (PPT) selection protocol, and the regeneration of plants are discussed. T0 and T1 plants were initially identified using the pH-dependent chlorophenol red test and/or the histochemical -glucuronidase (GUS) assay. PCR and Southern data confirm the presence of the 35S CaMV promoter and the synthetic Bt gene. 相似文献
86.
Summary A series of experiments was conducted to determine the variations arising between replicate selfing units, to compare self-fertility and cross-fertility in the same environment and to measure the variation in expression of self-fertility by different techniques and in different environments.Variation in self-fertility between selfing units within genotypes was relatively high, representing approximately one-third of the total observed variation in a series of duplicate selfings of over 60 genotypes tested in one environment. In order to differentiate clearly between the self-fertility of a limited number of genotypes as many as four replicates were needed, although this number varied with the environment in which the tests were conducted. Levels of self-fertility were similar in bag selfing units placed inside or outside a glasshouse in an average year, but were greater outside than inside in a year which was drier and warmer than average. In a highly humid environment the mean level of self-fertility attained was reduced by a factor of three. Removal of flag leaf laminae had an adverse effect on self-fertility, reducing it by half. Shaking the pollination bags, as is the usual practice to promote pollen dispersal, increased self-fertility very considerably over that of unshaken controls. Assisting pollen dispersal by blowing filtered air into the bags was time-consuming and did not prove to be superior to the shaking technique. It is clear that self-fertility can neither be considered in isolation from the environment in which it is determined, nor can it justifiably be related to cross-fertility unless both are determined in the same environment. A sample of over 60 genotypes tested in one environment was on average 2.2% as self-fertile as they were cross-fertile, with a range for individual genotypes from 0–31%. Optimum conditions for maximum expression of self-fertility, essential in the exploitation of this character in ryegrass breeding, have yet to be fully established. 相似文献
87.
88.
Quantitative trait loci (QTL) analysis was conducted to identify QTL for seed yield and color retention following processing
of a recombinant inbred line (RIL) black bean population. A population of 96 RILs were derived from the cross of black bean
cultivars ‘Jaguar’ and 115M and evaluated in replicated trials at one location over 4 years (2004–2007) in Michigan. A 119-point
genetic map constructed using simple sequence repeat (SSR), sequence related amplified polymorphism (SRAP), target region
amplified polymorphism (TRAP) and phenotypic markers spanned fifteen linkage groups (LG) or 460 cM of the bean genome. Fourteen
QTL for yield and color retention in four environments were identified by composite interval mapping on six linkage groups.
A major QTL SY10.2J115 for seed yield was identified on LG B10 with additional QTL on B3, B5, and B11. Color retention following processing was
associated with loci on B1, B3, B5, B8, and B11. 115M possessed positive alleles for yield, but negative alleles for color
retention. Some QTL for yield and color retention co-localized with regions identified in previous studies while others, particularly
for color retention, were unique. Additional QTL for agronomic and canning quality traits were detected and individual contributions
to future black bean breeding are discussed. 相似文献
89.
The addition of succinylsulfathiazole to highly purified diets containing all dietary factors known to be required by the rat, including pantothenic acid, results in the appearance of signs of severe pantothenic acid deficiency, including achromotrichia and porphyrin-caked whiskers. These changes are accompanied by a marked reduction in the pantothenic acid content of the liver, and are corrected by the inclusion in such diets of crystalline biotin and "folic acid" concentrates. The utilization of pantothenic acid by the rat appears to depend on the availability of biotin and "folic acid." Under normal conditions these are supplied by the diet and synthesized by intestinal bacteria. A hypothesis is offered in explanation of the chromotrichial actions of "folic acid," biotin and p-aminobenzoic acid. 相似文献
90.