叶面喷施丰产素在黄瓜上的应用效果     

石岭  尉红菊  王若菁  赵清岩 《内蒙古农业大学学报(自然科学版)》1994,(3)

本试验在黄瓜上叶面喷施丰州牌丰产素，其浓度分别为２０００倍、４０００倍和６０００倍。试验结果表明，３种浓度对黄瓜的生长势都有增强的作用，并对早期产量有较大的提高。其中２０００倍液对早期产量的增产幅度最大，比对照提高４２．８１％。并且对黄瓜的营养成份有不同程度的影响，其中６０００倍液对维生素ｃ和含糖量都有较大的提高，因此，对黄瓜品质的改进有较好的效果。  相似文献   

Correlated Responses in Two Traits when Selection is Practised on their Ratio     

M. Hühn 《Plant Breeding》1992,109(1):28-34

In many fields of application in plant breeding and crop science, ratios of two component traits X and Y are of interest (harvest index in cereals, leaf-to-stem ratio in forage legumes, height-to-diameter ratio in forest trees etc.). When selection is practised on the ratio X/Y of two traits X and Y, the experimenter may be interested in the resulting changes of both trait means. Based on improved approximations for the covariances between X and X/Y and between Y and X/Y and for the variance of X/Y the changes in the means of X and Y can be predicted by applying the regression approach from conventional selection theory. Explicit expressions for these correlated responses in X and Y when selection is practised on their ratio X/Y are derived and discussed. The different outcomes (decrease, zero change or increase) for the selection pressures on X and Y are characterized by phenotypic coefficients of variation of X and Y, phenotypic and genotypic correlations between X and Y and heritabilities of X and Y.  相似文献   

Determination of the protease activity in a Cuban strain of Babesia bovis]     

L C Savon  M Alonso  J Rodriguez-Diego  T Blandino 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1992,45(1):30-31

The attenuation of a Babesia bovis strain depends on its protease content. The present work evaluates this parameter on a virulent strain, before and after attenuation by quick passages on splenectomized calves. The protease activity at different pH values was determined in protein fractions from the blood of calves. The enzymatic test showed marked differences between the protease content of both substrains.  相似文献   

Lupus-Type "Anticoagulant" in a Dog With Hemolysis and Thrombosis   总被引：1，自引：1，他引：0  

Michael S. Stone  Ian B. Johnstone  Marjory Brooks  Trent K. Bollinger  Susan M. Cotter 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(1):57-61

A circulating anticoagulant was detected in a 2-year-old Chesapeake Bay Retriever with hemolytic anemia, nephrotic syndrome, thrombocytopenia, polyarthropathy, and pulmonary thromboembolism. A persistent prolongation of the activated partial thromboplastin time (aPTT) was detected, and it did not correct with repeated administration of fresh frozen plasma. The aPTT was still prolonged, with a 1:1 mixture of patient's plasma and normal dog plasma in vitro, suggesting the presence of a circulating inhibitor. Results of assays to characterize the inhibitor were compatible with those described for the lupus anticoagulant in human patients with systemic lupus erythematosus. Paradoxically, patients having the lupus anticoagulant are at increased risk for thrombosis. Pulmonary thromboembolism has been described as a frequent complication of immune-mediated hemolytic anemia in the dog, and the presence of a circulating anticoagulant should be considered as a potential mechanism.  相似文献   

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.   总被引：2，自引：0，他引：2       下载免费PDF全文

J Q Xia  C V Yason    F S Kibenge 《Canadian journal of veterinary research》1995,59(2):102-109

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

USE OF MONOCLONAL ANTIBODY GB 24 and PROPIDIUM IODIDE FOR SIMULTANEOUS ASSESSMENT OF ACROSOMAL STATUS and VITALITY IN BOAR SPERMATOZOA     

P. Renard  B. Drénou  J.F. Griveau  D. Le Lannou 《Reproduction in domestic animals》1995,31(1):255-256

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Temporary Bile Diversion in Cats with Experimental Extrahepatic Bile Duct Obstruction     

DARIEN LAWRENCE BSc  BVSC    JAMIE R. BELLAH DVM  Dipiomate ACVS    D. J. MEYER DVM  Dipiomate ACVIM  Dipiomate ACVP  L. ROTH DVM  PhD  Dipiomate ACVP 《Veterinary surgery : VS》1992,21(6):446-451

The use of a cholecystostomy catheter for temporary bile diversion was investigated in four cats with experimentally induced extrahepatic bile duct obstruction. Eighteen days after ligation of the common bile duct, a 6.5 F accordion catheter was placed in the gallbladder with a 22 g Hawkins needle-guide system through a paracostal incision. Biochemical parameters and fasting serum bile acids were monitored for 16 days. There were significant decreases in mean total bilirubin, aspartate aminotransferase, and fasting serum bile acids within 72 hours of bile diversion, and in mean alanine aminotransferase within 96 hours. Attitude and appetite improved, and the catheter was tolerated well. Positive bile cultures developed in three cats. Histologic changes in the gallbladder included mucosal ulcerations, a mixed inflammatory cellular infiltration, and fibrosis of the submucosa.  相似文献   

Effect of inoculum density of Macrophomina phaseolina on seedling susceptibility of six conifer species     

A. H. McCain  R. F. Scharpf 《Forest Pathology》1989,19(2):119-123

The severity of charcoal root disease of conifer seedlings (Abies concolor, A. magnified, Pinus jeffreyi, P. lambertiana, and Pseudotsuga menziesii) was a power function of the sclerotial density of the fungus (Macrophomina phaseolina) in nursery soil.  相似文献   

马铃薯品种抗软腐病测定条件的研究     

张学君  王金生 《南京农业大学学报》1989,12(1):67-72

用全薯块吸头刺伤接种法测定时,较理想的测定条件为:每接种点接种0.1ml浓度为10~2～10~5CFU/ml菌悬液,随后在21±5℃～31±5℃(依所用菌株而定)下保持2～3天,以接种点腐烂斑的最大直径作记载标准。用新鲜薯片法测定时,除接种浓度为10~2～10~3CFU/ml,保持时间为1～2天及以侵染限值作评价标准外,其余条件和全薯块吸头刺伤接种法相同。当用皮孔浸渍法接种时,薯块在10_6CFU/ml菌悬液中浸5分钟,然后使薯块表面保持连续水膜,3～5天后测量薯块表面腐烂面积。上述3种方法都必须有20个以上重复,并使用大小一致、无伤无病的成熟薯块。  相似文献   

Quantitative Aspects of Thyroid Scintigraphy With Pertechnetate (^99mTcO₄) in Cats     

Ariana M.P. Nap  Yvonne W.E.A. Pollak  Walter E. van den  Brom Ad Rijnberk DVM  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):302-303

Thyroidal ^99mTcO₄(pertechnetate) uptake percentages were determined in unanesthetized euthyroid (n = 13) and hyperthyroid (n = 18) cats. Maximal uptakes were observed 60 minutes after IV injection of the radionuclide and ranged from 0.3 to 3.9% of the dose in euthyroid cats (median 2.23%) and from 5.2% to 23.9% of the dose in hyperthyroid cats (median 14.8%) ( P < .05). There were no overlaps in pertechnetate uptake percentages during any of the intervals evaluated. It is concluded that the optimal time for visualization of the thyroid by ^99mTcO₄-scanning is 60 minutes after IV injection of the radionuclide. Calculation of the percentage uptake is of additional diagnostic value.  相似文献