张家山水库化肥养鱼增产试验   总被引：1，自引：0，他引：1  

库么梅  何绪刚 《湖北农学院学报》1994,14(3):45-49

张家山水库位于湖北江陵境北，面积２１６．７ｈｍ￣２，水深２．５～３．０ｍ，是一中型贫营养型水库。通过实施化肥养鱼，产量由１９９２年的１１０．０ｋｇ／ｈｍ￣２提高到１９９３年的２１３．０ｋｇ／ｈｍ￣２，鲢鳙的上市规格分别由１９９２年的０．５ｋｇ／尾、１．５ｋｇ／尾提高到１９９３年的０．８ｋｇ／尾、２．５ｋｇ／尾，取得了高产试验的成功。  相似文献   

滁县地区粮食生产发展措施与区域性发展模式的研究     

许刚  吴豪 《安徽农业科学》1993,21(2):97-101

本文系统论述了滁县地区粮食生产发展措施,确立了区域农业综合发展模式,并阐述了该模式的实质、意义、运行机制和实施策略。  相似文献   

Pyogenic bacterial infections in humans with MyD88 deficiency     

von Bernuth H  Picard C  Jin Z  Pankla R  Xiao H  Ku CL  Chrabieh M  Mustapha IB  Ghandil P  Camcioglu Y  Vasconcelos J  Sirvent N  Guedes M  Vitor AB  Herrero-Mata MJ  Aróstegui JI  Rodrigo C  Alsina L  Ruiz-Ortiz E  Juan M  Fortuny C  Yagüe J  Antón J  Pascal M  Chang HH  Janniere L  Rose Y  Garty BZ  Chapel H  Issekutz A  Maródi L  Rodriguez-Gallego C  Banchereau J  Abel L  Li X  Chaussabel D  Puel A  Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696

MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections.  相似文献   

Initiation of sulfate activation: a variation in c4 photosynthesis plants     

Gerwick BC  Ku SB  Black CC 《Science (New York, N.Y.)》1980,209(4455):513-515

In leaves of plants with C(4) photosynthesis, sulfur assimilation is initiated in bundle sheath cells whereas carbon and nitrogen assimilation are initiated in mesophyll cells. The activation of sulfate by adenosine triphosphate sulfurylase in leaves of C(4) plants occurs in chloroplasts of bundle sheath cells and is effected by two isozymes of approximately equal activities that accounted for 95 to 100 percent of the total leaf activity.  相似文献   

利用棉绳采集口腔液监测猪伪狂犬病方法的应用分析     

于学祥  许拓  何东贤  库旭钢  罗锐  范盛先  何启盖 《中国畜牧兽医》2021,(1)

为分析使用棉绳采集口腔液监测猪场伪狂犬病(PR)方法的科学性,本研究拟通过实验室水平、动物试验及临床应用3个方面对使用棉绳采集口腔液监测猪场PR这一方法开展应用分析。首先对采样条件进行优化,并通过模拟试验测定棉绳对伪狂犬病病毒(PRV)的释放能力,通过动物试验测定病毒感染后检出时间,最后对临床样品进行检测分析。结果表明:使用直径为1.0 cm的棉绳,在早上喂料前采集口腔液样品,采样时间为20~30 min,可采集到更能满足试验要求的口腔液。病毒释放能力测定显示,棉绳对PRV的释放能力为50%左右,使用棉绳采集口腔液可检测到1个TCID50/0.1 mL的病毒含量。动物试验检测发现,猪群在感染后28 d中除第5天外,口腔液中病原含量均高于鼻拭子中病原含量,口腔液检测效果优于鼻拭子,且口腔液中病毒的检出时间(感染后第1天)早于血液中抗体转阳时间(感染后第7天)。临床样品检测分析结果表明,PRV疫苗免疫后也可通过口腔液检测到疫苗毒,并且存在无法通过口腔液检测感染PRV野毒后稳定猪中带毒的情况,因此口腔液检测方法应结合gE抗体检测,才可综合判断猪群是否为感染群体。综上,本研究优化了口腔液采集方法,并测定了棉绳对口腔液的释放能力和感染后检出时间,表明口腔液可作为较好的监测猪群PR的手段;口腔液监测方法需结合gE抗体检测来综合判断猪群是否为感染群体。  相似文献   

基于景观格局分析的兴国县土壤侵蚀演变研究   总被引：17，自引：1，他引：17  

王库史学正  于东升史德明 《水土保持学报》2003,17(4):94-97,116

利用1958年、1975年、1982年和1996年的土壤侵蚀强度等级图,在GIS软件支持下生成土壤侵蚀强度栅格分类图。通过FRAGSTATS景观格局分析软件计算出了10多种景观类型参数,并从斑块类型水平和景观水平2个层次上定量地分析了兴国县土壤侵蚀的分布格局及其变化规律。结果表明:兴国县的土壤侵蚀状况从1958年到1996年已发生了明显的好转。此期间经历了两个明显的时期:即1958年到1975年的侵蚀加剧时期和1975年后的侵蚀减退期。到1996年,兴国县无明显侵蚀的面积已达到全县面积的74.6%,严重土壤侵蚀状况已基本得到了控制。  相似文献   

Development of a molecular marker for a bruchid (Callosobruchus chinensis L.) resistance gene in mungbean     

Huei-Mei Chen  Chien-An Liu  C. George Kuo  Ching-Mei Chien  Horng-Chi Sun  Chung-Chu Huang  Yu-Chung Lin  Hsin-Mei Ku 《Euphytica》2007,157(1-2):113-122

Bruchid, Callosobruchus spp. (Coleoptera: Bruchidae), is a serious pest during storage of seeds of mungbean (Vigna radiata (L.) Wilczek) and other Vigna species. A source of resistance to this pest has been identified in Vigna sublobata (Roxb.) Bairig. accession TC1966. Two hundred recombinant inbred lines at the F₁₂ generation have been developed for molecular mapping of bruchid resistance (Br) gene in TC1966. Through bulked segregant analysis (BSA), ten randomly amplified polymorphic DNA (RAPD) markers associated with the bruchid resistance gene were successfully identified. A total of four closely linked RAPDs were cloned and transformed into sequence characterized amplified region (SCAR) and cleaved amplified polymorphism (CAP) markers. Seven CAPs developed from the identified RAPD markers showed tighter linkage with the Br gene than the original RAPD. Through transformation of RAPDs into CAPs, codominant markers for bruchid resistance were successfully obtained. Homozygous genotypes of these PCR-based markers were estimated to contribute 85% of the variance for seed damage when the insect assay was performed under favorable growth conditions for bruchid.  相似文献   

面向池塘圈养模式的气液混合泵的参数优化及增氧试验     

裴凯  王永江  黄庆兰  朱明  何绪刚  冯耀泽 《华中农业大学学报》2024,43(2):40-46

为探究气液混合泵能否满足在高密度养殖条件下养殖对象对水体溶氧的需求,基于气液（氧气-水）混合泵搭建溶氧试验平台,在不同水温、不同出水压力和不同气水体积比的条件下,测试气液混合泵溶氧性能,并在池塘圈养桶（直径4 m,高2 m,养殖水体体积20 m³）内进行增氧试验。溶氧性能测试结果显示：当出水压力为0.25 MPa、气水体积比为0.01~0.05时,在不同水温（5.6、13.5、30.3）条件下出水溶解氧与水温成反比,溶解氧在47.93~20.60 mg/L变化;氧气吸收效率与气水体积比呈反比,氧气吸收效率在91%~33.7%变化;动力效率与气水体积比成正比,动力效率在22.32~55.12 kg/（kW·h）变化。基于圈养桶的增氧试验结果显示,在有鱼耗氧的条件下（黄颡鱼,单个桶内养殖密度为13.19~16.49 kg/m³）,使用功率3 kW的气液混合泵为4个圈养桶增氧时,每个桶内水体溶解氧在光照时间内可达11 mg/L,夜间稳定保持在8 mg/L以上。试验结果表明气液混合泵可应用于高密度的水产养殖,并能有效应对夏季高温供氧难题。  相似文献   

一例猪瘟混合感染副猪嗜血杆菌和猪蛔虫的案例诊治     

陈晓雨  于学祥  孙琪  库旭钢  范盛先  何启盖 《猪业科学》2020,37(7):76-80

2019年11月底,湖北某商品猪场保育猪只出现厌食、消瘦、呼吸困难、咳嗽等症状,偶有病猪急性死亡,为查找病因,控制疫情,减少损失,依次通过流行病学调查、病理剖检和实验室检测,最终确定该发病猪场为感染猪瘟病毒,进而继发感染Hps-4混合感染,并伴随蛔虫感染导致。通过筛选敏感药物治疗,优化生产管理,成功控制了此次疫情。该案例对此次疫情进行了分析总结,就加强生物安全建设,优化养殖管理,实现生产质量可控提供了参考建议。也为猪瘟混合感染呼吸系统细菌性疾病的防控提供参考。  相似文献   

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)     

WANG Shun-li  Seong Sub Ku  YE Xing-guo  HE Cong-fen  Suk Yoon Kwon  Pil Son Choi 《中国农业科学(英文版)》2015,(3):469-482

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.  相似文献