用苄基腺嘌呤打破苹果胚休眠     

Zhang Y X  Lespinasse Y  薛进军 《中国果树》1992,(3)

<正> 从未层积的金冠苹果种子里取出的胚用12.5—25.0 mg/L苄基腺嘌呤(BA)浸泡6—24 h(小时)或50—100 mg/L浸泡1—24 h后很快就萌芽。低浓度(12.5—25.0 mg/L)处理效果好于高浓度(50—100 mg/L)处理,植株生长更为正常。与BA比  相似文献   

胚胎干细胞定向分化研究进展(上)     

张斌  华修国  朱淑文 《现代畜牧兽医》2003,(5):40-40

胚胎干细胞 (ES细胞 )是由哺乳动物早期胚胎分离克隆的一类未分化二倍体细胞 ,能在体外增殖 ,又能保持未分化状态。在一定条件下 ,ES细胞可向多个方向分化 ,并生成多种功能细胞。ES细胞在遗传上具有可操作性 ,即导入外源基因、标志基因和报告基因 ,诱导基因突变 ,基因打靶或导入额外原有基因使之过度表达等。利用该特性可制作各种实验模型并进行各种基因分析。ES细胞能被诱导分化为构成机体的任一种细胞类型 ,可用于临床细胞、组织和器官的修复和移植治疗 ,研究ES细胞的定向分化有着重要的理论和应用价值。1　ES细胞分化机制研究现状目…  相似文献   

饲料企业应该如何准确评估饲料饲养效果     

张颖  肖凯  胡晓艳  燕富永  肖劫  龚斯  张斌  肖淑华 《饲料广角》2009,(23):19-21

饲养试验是动物营养和饲料研究中应用最广泛、最常用也是最基本的试验方法，它通常将动物进行分组或分期饲喂，每组或每期分别进行不同的处理，如喂以不同质量的饲料，在其他条件一致时，饲喂一定时期后，比较各组动物或者各期动物的生产性能的高低，以评价不同质量饲料营养价值的高低或确定动物对营养物质的需要量。  相似文献   

可可茎尖培养     

Adu－A.  Y 王海宁 《云南热作科技》1994,17(2):38-43

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是猪场的经营管理水平高低而非规模大小影响着猪场的盈利与否     

吴昊杰  张斌  Rod Smith 《国外畜牧学(猪与禽)》2003,23(1)

根据美国伊阿华州养猪业记录系统的数据,是猪场管理的好坏,而非猪场规模的大小,造成了猪场盈利与否的差别,而且这一差别是非常显著的.  相似文献   

用含有凝胶的基质进行等电点聚焦电泳分离和检测蛋白酶     

Yamau.  Y 朱克寅 《农业新技术新方法译丛》1996,(2):1-2

介绍用等电点聚焦电泳分离并检测蛋白酶的一种精确方法。向含有明胶铁聚丙烯酰胺凝胶中加入０．３％辛基－Ｄ－吡喃葡糖苷或８Ｍ尿素，同时向样品溶液中加入细胞色素Ｃ和ＰＩ标记蛋白，这样可避免电泳过程中蛋白酶与基质的互作。在木瓜蛋白酶，胰凝乳蛋白酶和蛋白酶Ｋ等常用蛋白酶的估计等电点进行检测，检测极限可达微微克至毫微克。  相似文献   

Mastitis Caused by Haemophilus somnus in a Dairy Cow   总被引：2，自引：1，他引：1  

Higgins R  Martin JR  Larouche Y  Goyette G 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(3):117-119

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Key problems in science and technology in Thailand     

Yuthavong Y  Sripaipan C  Kirtikara K  Glankwamdee A  Trakulku K 《Science (New York, N.Y.)》1985,227(4690):1007-1011

Science and technology can make substantial contributions to socio-economic development in Thailand. In order to promote such contributions, problem areas and specific problems of high priority need to be defined, and possible solutions outlined. Important criteria for selecting priority areas and key problems include relevance to development, the availability of human resources and the possibility of their development, the cutting-edge advantage to be gained by development, the possibility of international collaboration, and the possibility of integration with culture and environment. These criteria suggest in Thailand a focus on the development of bioscience and biotechnology, materials science, electronics, and information science.  相似文献   

Coral community reproductive patterns: red sea versus the great barrier reef     

Shlesinger Y  Loya Y 《Science (New York, N.Y.)》1985,228(4705):1333-1335

In contrast to many corals of the Great Barrier Reef, Australia, which are synchronous multispecific spawners, the abundant coral species in the northern Red Sea, Israel, exhibit temporal reproductive isolation. Spawning dates of 12 of the 13 Red Sea species followed lunar periodicity and were consistent throughout 3 years of study. Spawning periods of all species occurred in different seasons, different months, or different lunar phases within the same month. The high abundance of the corals studied at Eilat may be due in part to the advantages gained through not having overlapping spawning periods and settlement times.  相似文献   

Biochemical polymorphisms in Spanish Avileña-Negra Iberica cattle     

Arranz JJ  Bayón Y  Medjugorac I  Primitivo F 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》1994,111(1-6):413-417

SUMMARY: Thirteen biochemical blood polymorphisms were analysed in a population of 149 Spanish Avile?a-Negra Ibérica cattle. The study revealed variation at the following nine loci: HBB, CA, NP, CP, AMY1, ALB, GC, TF and PTF2. The following systems were monomorphic: CAT, DIA1, MDH1 and ME1. Using polyacrylamide-gel electrophoresis, a slow, migrating pair of bands was found in the GC protein system. This pattern is probably controlled by the GC(C) allele, described in only a few cases in cattle. Furthermore, starch-gel electrophoresis allowed the detection of a variant with intermediate mobility between the ALB(A) and the ALB(B) alleles at the albumin locus. A variant with a similar electrophoretic pattern has occasionally been reported in cattle. However, utilizing IEF under denaturing conditions, such a variant could not be differentiated from the ALB(A) allele and thus its significance is not clear. ZUSAMMENFASSUNG: Biochemischer Polymorphismus in spanischen Avile?a-Negra Iberica Rindern Insgesamt 13 biochemische Systeme wurden in einer Population von 149 spanischen Avilena-Negra-Iberika-Rindern hinsichtlich Polymorphismus analysiert. Es zeigten sich Varianten bei folgenden Loci: HBB, CA, NP, CP, AMY1, ANB, GC, TF und BTF2, w?hrend CAT, DEA, MDH1 und ME1 monomorph sind. Bei st?rke Gel-Elektrophorese wurde im Albumin-Locus eine Variante mit intermedi?rer Mobilit?t zwischen ALB(A) und ?LB(B) Allel entdeckt. Eine solche Variante wurde bisher nur sehr selten bei Rindern beobachtet. Darüber hinaus wurde bei Polyacrylamid-Gel-Elektrophorese ein langsam wanderndes Paar von B?ndern im GC-Proteinsystem gefunden. Dieses Muster ist wahrscheinlich von dem selten vorkommenden GC(C) -Allel verursacht. RESUMEN: Se analizaron trece polimorfismos bioquímicos sanguíneos en una población de 149 animales de la raza Avile?a-Negra Ibérica de ganado vacuno. El estudio reveló la existencia de variación en los nueve loci siguientes: HBB, CA, NP, CP, AMY1, ALB, GC, TF y PTF2. Fueron monomórficos los sistemas siguientes: CAT, DIA1, MDH1 y ME1. Utilizando eletroforesis en gel de poliacrilamida se encontró un par de bandas de migración lenta en el sistema de la proteína GC. Este patrón probablemente está controlado por el infrecuente alelo GC(C) , descrito en unos pocos casos en el ganado vacuno. Además, la electroforesis en gel de almidón permitió detectar en el locus de la albúmina una banda con movilidad intermedia entre los alelos ALB(A) y ALB(B) . Una variante con un patrón electroforético similar ha sido descrita en muy pocas ocasiones en el ganado vacuno. Sin embargo, la utilización de IEF en condiciones desnaturalizantes no permitió diferenciar esta variante del alelo ALB(A) y, por lo tanto, el significado de la misma no está claro.  相似文献