Effect of One-off Application of Poly Urea-Formaldehyde Fertilizer Under Reduced N Rate on Double Cropping Rice   总被引：2，自引：0，他引：2  

QiaoYi HUANG  Mu ZHANG  Xu HUANG  ShuanHu TANG  FaBao ZHANG  YuWan PANG  Qiong YI  Ping LI  HongTing FU 《中国农业科学》2018,51(20):3996-4006

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Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells     

LI Fu-hai  LI Zong-zhuang  JIANG Zhi  YI Wei  ZHANG Chen-yun 《园艺学报》2015,31(6):1130-1136

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.  相似文献   

Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms     

YI Ji-hai  ZHANG Jun-bo  LI Shuang  WANG Xi-chu  ZHANG Hui  CHEN Chuang-fu 《中国畜牧兽医》2016,43(3):592-600

By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.  相似文献   

农业科研单位编制外用工的管理策略     

陈洁  张智  蚁秀清 《热带作物学报》2016,(3):72-74

编制外用工是农业科研单位普遍存在的用工形式。本文阐述了农业科研单位编制外用工的特点，分析了编制外用工存在问题及原因，提出从规范用工管理方式、强化考核管理、拓宽发展空间等方面提高编制外人员的工作积极性，提升编制外用工人力资源管理的效能，促进单位发展。  相似文献   

不同耕作方式对辽西褐土物理性状及玉米根系分布的影响     

李嵩  韩巍  张凯  依艳丽 《玉米科学》2020,28(6):101-106

通过连续3年大田试验，对旋耕、翻耕、深松3种耕作方式下的土壤物理性质、玉米根系分布和产量进行测定。结果表明，与翻耕和旋耕相比，深松显著增加了玉米田土壤耕层厚度和降低了犁底层厚度。在中下层土壤，深松还降低了土壤紧实度和容重，改善了土壤的孔隙状况，有利于玉米根系向下生长，使得中下层土壤的玉米根系不仅更丰富，而且占总根系量的比例也更高，最终提高了玉米产量。本研究表明，深松耕作有利于改善辽西褐土区土壤结构和促进玉米生长。  相似文献   

新疆日光温室前屋面的风载数值模拟分析     

马月虹  李保明  王国强  宋兵伟  刘娜 《新疆农业科学》2020,57(12):2325-2331

【目的】 对日光温室前屋面风载大小和变化趋势进行模拟,可辅助前屋面骨架结构优化设计和前屋面实际风载计算,为提前预测和防灾功能提供科学依据。【方法】 将日光温室墙体、保温被和骨架等结构简化,使用UG 软件对日光温室进行造型,将模型与空气进行布尔求差,得到整体模型,将整体模型导入到ANSYS ICEM中,进行网格划分,得到保温被4种工作状态薄膜风压。再通过Y向的9条曲线画出不同条件下风压变化对比曲线。【结果】 在无保温被和保温被全铺时,薄膜最南侧风压最高,约1.014×10⁵Pa,往北侧风压逐渐降低,脊高位置风压最小约1.010×10⁵Pa。由于最南侧风遇到薄膜阻碍,造成风压的升高,而北侧接近日光温室顶端,风速快,而风速快的位置风压小。在保温被全卷及半卷时,也是最南侧风压较最高,约1.014×10⁵Pa,往北有风压的降低,总体屋面风压变化比较小,约1.013×10⁵Pa。只是保温被半卷时,在保温被卷放位置会有风压的突变为1.012×10⁵Pa。【结论】 无保温被、保温被全铺、保温被全卷及保温被半卷4种情况南侧风压较大且相差不大,但越往北侧,保温被全卷或半卷会造成薄膜风压的升高,尤其是顶部的突然升高。保温被全铺是最佳防风方案。南侧风压高,南侧前屋面钢骨架拱形段的性能对日光温室结构稳定性和安全性至关重要,设计和建设时需要着重计算和核验。  相似文献   

绿茶面条的制作及其微观结构与茶多酚含量的变化     

韦玲冬  易霞  邹溪  杨小妹  吕辉 《贵州农业科学》2020,48(10):135-139

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改性尿素追施对冬小麦和夏玉米季氮素挥发和淋溶的影响     

肖强  李丽霞  李鸿雁  衣文平  邹国元  刘建斌  孙世友 《水土保持学报》2020,34(4):270-279

以NBPT(N-丁基硫代磷酰三胺)和DMPP(3,4-二甲基吡唑磷酸盐)为复合抑制剂,利用转鼓喷涂工艺,开发出新型复合型抑制剂涂覆尿素肥料,采用扫描电镜和能谱仪分析其涂覆效果。通过田间试验系统对比评价了追施不同氮肥对调控氮素的特征效果。试验设置5个处理:(1)不施氮肥(CK);(2)农民习惯追施尿素(CU);(3)优化追施尿素(CUU);(4)优化追施抑制剂涂覆尿素(CUY1);(5)优化追施抑制剂涂覆尿素(CUY2)。在夏玉米喇叭口期、冬小麦拔节期追施氮肥后的15天内进行田间原位连续动态观测。电镜和能谱结果表明,复合抑制剂均匀涂覆于尿素表面,形成薄而致密、光滑的涂覆层,该涂覆层均匀分布有磷和硫2种元素,表明复合抑制剂与尿素已有效结合。田间试验结果表明,在同等优化施氮量下与普通尿素相比,夏玉米和冬小麦季追肥后CUY1和CUY2处理氨挥发分别降低55.19%,32.15%和52.46%,39.43%。夏玉米季追肥后,0-20 cm土层CU、CUU处理土壤硝态氮含量于第5天达到峰值,到后期已显著低于CUY1、CUY2处理,CUY2处理稳定硝态氮的效果更好。冬小麦季追肥后,0-20 cm土壤硝态氮含量CU、CUU处理分别在第5,3天达到峰值,CUY1、CUY2处理于第11天达到峰值后,硝态氮含量已显著高于相同施氮量的CUU处理。在保证产量和净收益的同时,抑制剂涂覆尿素显著降低了追施氮素的氨挥发和淋溶损失浓度,其中冬小麦季CUY1处济效益较好,夏玉米季CUY2调控氮素的效果最佳,减少向下淋溶的效果明显。  相似文献   

湿地松产脂量与生长及树冠性状多点遗传相关及通径分析     

冷春晖  张露  易敏  孙世武  蒋祥英  赖猛 《核农学报》2020,34(7):1598-1605

为研究湿地松生长及树冠性状与产脂量之间的遗传相关关系及其对产脂量的控制途径,以赣北、赣中和赣南等多种典型立地条件下的28年生湿地松家系试验林为研究对象,对112个湿地松家系进行产脂量、生长及树冠性状全林调查,并对测定数据进行分析。结果表明,除枝下高外,产脂量与生长及树冠性状均呈显著或极显著遗传正相关关系,遗传相关系数排名前四的性状分别为胸径(R=0.93)、树冠表面积(R=0.83)、冠长(R=0.78)和冠幅(R=0.73);通径分析中,胸径、树高、树冠表面积和冠幅通过直接和间接作用成为影响产脂量的主导因素,其决定系数贡献率分别为0.557、0.507、0.424和0.240;产脂量与生长、树冠性状的线性回归方程为:y=-0.255+0.040x_(DBH)+0.004x_(HGT)+0.006x_(CSA)+0.036x_(CW)(F=955.907^**, R²=0.559),模型的预估精度为99.64%。本试验结果明确了湿地松生长及树冠性状与产脂量之间的关系,其中,树高、胸径、冠幅和树冠表面积对产脂量影响较大;建立的产脂量多元回归模型具有一定的现实意义,实现了对树高、胸径和树冠表面积等性状的快速测定预估产脂量,为湿地松产脂量的科学、准确与高效预测提供了依据。  相似文献   

蔬菜农药残留检测中基质效应影响因素分析     

范叶  易泽夫  王壮波 《蔬菜》2019,(2):60-64

为了提高农药检测的准确率,试验选择气相色谱(7890A、1310)与气相串联质谱(GCMS-MS)3种不同仪器,结球白菜、结球甘蓝、黄瓜、辣椒4种蔬菜样品及其基质标准溶液和正己烷溶剂标准溶液,对4种不同蔬菜中分别添加腐霉利、甲氰菊酯、氯氰菊酯、溴氰菊酯4种农药并进行回收校正,比较不同的校正溶液、不同仪器以及不同的样品类型对蔬菜农药残留检测中的基质效应的影响。结果显示:按不同基质标准溶液和溶剂标准溶液校正的检测结果分类,不同蔬菜、不同的标准校正溶液对于农药残留检测中的基质效应表现出多样性和复杂性的特点。但采用与本底一致的基质标准溶液校正样品可有效消除基质效应。按不同仪器检测结果分类,气相色谱(7890A、1310)与气相串联质谱(GC-MS-MS)基质效应的影响存在一定的差异,GC-MS-MS存在较为明显的基质增强效应;而同类仪器(7890A、1310)之间基质效应的影响较小。  相似文献