Expression and function of calcium-sensing receptor in mouse embryonic stem cells     

SUN Jian  YU Jin-feng  LUAN Hai-rong  LI Shuang  XU Xiao-yi  HE Zhi-peng  WEI Tao  LI Li  XU Chang-qing 《园艺学报》2014,30(4):651-657

AIM: To observe the functional expression of calcium-sensing receptor (CaSR) in the mouse embryonic stem cells (mESCs). METHODS: The expression and distribution of CaSR were detected by Western blotting and immunofluorescence observation in 129 mouse ES-D3 cells. The intracellular concentration of free calcium (［Ca2+］i) was determined by confocal laser scanning microscopy. The cell viability was analyzed by MTT assay and flow cytometry. RESULTS: CaSR protein was expressed in mESCs. Extracellular calcium or neomycin significantly increased the expression of CaSR and ［Ca2+］i. Neomycin increased the cell viability, up-regulated the protein expression of p-ERK2. These effects of neomycin were inhibited by NPS2390. CONCLUSION: CaSR is expressed in mESCs. The activation of CaSR is involved in the proliferation of mESCs.  相似文献   

RASSF1A expression mediated by lentivirus inhibits growth of small-cell lung cancer cell line H446     

KONG Li-jun  ZHANG Li-xia  LUAN Xi-ying  ZHANG Li-sha  WANG Xu-han  YU Heng-yun  ZHANG Yue-zhi 《园艺学报》2014,30(7):1209-1213

AIM：To explore the inhibitory effect of Ras-association domain family 1A (RASSF1A) on the small-cell lung cancer cell growth. METHODS：The lentiviral expression vector containing RASSF1A gene was constructed and used to infect the small-cell lung cell line H446. The growth curve and cell cycle were detected by MTT assay and flow cytometry. The mRNA and protein levels of cell cycle-associated proteins were determined by real-time PCR and Western blotting. RESULTS：We obtained the H446 cells in which RASSF1A was stably expressed (named RASSF1A-H446). Compared with normal cell group and negative cell group, RASSF1A inhibited the proliferation of H446 cells, and arrested H446 cells in G1 phase. The expression of p21 and p27 was significantly increased, and E2F1 was significantly decreased in RASSF1A-H446 cells. CONCLUSION：RASSF1A inhibits the H446 cell growth by increasing the expressions of p21 and p27, and decreasing the expression of E2F1.  相似文献   

Vasonatrin peptide attenuates injuries of dopaminergic neurons induced by 1-methyl-4-phenylpyridinium     

YU Jun  CHEN Bao-ying  ZHAO Ge  ZHANG Xue-ce  ZHAO Hai-kang 《园艺学报》2014,30(5):832-836

AIM：To investigate the neuroprotective effects of vasonatrin peptide (VNP) on the injury of dopaminergic neurons induced by 1-methyl-4-phenylpyridinium (MPP+). METHODS：Cultured dopaminergic neurons from the mouse ventral mesencephalon were exposed to MPP+, and the effects of VNP on the neurotoxicity of MPP+ were eva-luated by cell viability analysis and immunofluorescence staining. Various kinds of agonists and antagonists were used to clarify the mechanism underlying the effects of VNP. RESULTS：MPP+ caused injuries in the dopaminergic neurons. VNP significantly increased the viability, axon number and axon length of the dopaminergic neurons. The MPP+-induced depolymerization of β-tubulin Ⅲ was also attenuated by the treatment with VNP. In addition, VNP significantly increased the intracellular levels of cGMP. These effects of VNP were mimicked by 8-Br-cGMP (a cell-permeable analog of cGMP), whereas inhibited by HS-142-1 ［the antagonist of the particulate guanylyl cyclase-coupled natriuretic peptide receptors (NPR)］, or KT-5823 ［a cGMP-dependent protein kinase (PKG) inhibitor］. CONCLUSION：VNP attenuates the neurotoxicity of MPP+ via guanylyl cyclase-coupled NPR/cGMP/PKG pathway, indicating that VNP might be a new effective reagent in the treatment of neural degeneration of dopaminergic neurons in Parkinson disease.  相似文献   

Hydrogen sulfide ameliorates high glucose-induced endothelial cell senescence by suppressing oxidative stress     

SONG Zhi-ming  WANG Min  LIU Yong  HAO Bao-shun  NIU Hai-ming  LIU Ding-hui  YU Shu-jie  ZHOU Bin  WU Lin  YU Xian-guan  LING Ye-sheng  PENG Pei  ZHU Jie-ming  CHEN Lin  QIAN Xiao-xian 《园艺学报》2014,30(8):1345-1350

AIM：To explore the effect of hydrogen sulfide on the senescence of human umbilical vein endothelial cells (HUVECs) induced by high glucose. METHODS：Senescence model was established by treating HUVECs with 33 mmol/L glucose for 48 h. The parameters were detected to demonstrate the effect of hydrogen sulfide on senescence and the mechanism involved was also investigated. RESULTS：In the cells treated with high glucose, the proliferation was attenuated with a higher number of senescence-associated β-galactosidase (SA-β-Gal) positive cells, and plasminogen activator inhibitor 1 (PAI-1) protein expression, malondialdehyde (MDA) production and NF-κB p65 activity were increased significantly, but the expression of superoxide dismutase 1 (SOD1) was decreased. However, the cell number and SOD1 expression were increased, and the number of SA-β-Gal positive cells, PAI-1 protein expression, MDA production and the activity of NF-κB p65 were decreased after sodium hydrosulfide (100 and 200 μmol/L) treatment.CONCLUSION：Exo-genous hydrogen sulfide prevents HUVECs against high glucose-induced senescence by suppressing oxidative stress and NF-κB p65 activity.  相似文献   

不同时期接种AM 真菌对大棚黄瓜产量及品质的影响     

李聪  贺超兴  闫妍  李衍素  于贤昌  Martina Janouskova Miroslav 《中国蔬菜》2014,1(8):21

研究不同时期接种丛枝菌根真菌根内球囊菌（Glomus intraradices,GI）对大棚土壤栽培黄瓜植株生长、产量及营
养品质的影响。结果表明：黄瓜不同时期接种GI 对其生长均有促进作用,与不接菌的对照相比,播种时接菌和移栽时接菌处理使黄瓜的单株产量分别提高54%、34%;播种时接菌处理的黄瓜叶片的叶绿素含量比对照显著提高了14.7%～17.7%;播种时接菌和移栽时接菌的黄瓜硝酸盐含量较对照分别下降了9.4% 和15.0%,总糖含量分别比对照提高了8.9% 和7.1%;移栽时接菌处理的黄瓜粗蛋白含量比对照提高了4.6%;播种时接菌和移栽时接菌显著提高了黄瓜VC 含量。表明接种GI 对黄瓜生长和产量品质具有一定的改善作用。  相似文献   

小株采种阳畦中白菜幼苗症状的识别     

张德双  汪维红  张凤兰  余阳俊  赵岫云  于拴仓  苏同兵  卢桂香 《中国蔬菜》2014,1(6):74

目前,小株采种是白菜杂交一代种子生产的主要方式,而播种和育苗环节是小株采种的两个关键技术环节,阳畦育苗过程中如何识别幼苗受害症状至关重要。  相似文献   

甘肃高原夏菜产地预冷与保温物流模式分析     

冯毓琴  张永茂  于洋 《中国蔬菜》2014,1(12):61

针对甘肃高原夏菜产地分散,冷库预冷能力弱、缺乏冷链运输设备的情况,提出发展
组装式小冷库、改进差压预冷技术以及采用蔬菜绿色包装和促进冷链物流的发展建议。  相似文献   

蒋卫杰博士：聚焦生产一线（五）——凌源市日光温室甜椒长季节栽培技术     

司海静  杨贺  蒋卫杰  余宏军 《中国蔬菜》2014,1(11):83

辽宁省凌源市丰富的光照资源为日光温室的发展创造了条件。目前凌源市日光温室甜椒生产面积达1 333 hm²（2 万亩）,通过选用优良品种,进行工厂化育苗,田间配以精细栽培管理,日光温室冬春茬甜椒每667 m² 年产量达1.5 万kg,获得了较高的产量和效益。  相似文献   

春季栽培甜玉米品种鄂甜玉6号的种植密度筛选试验     

余才良  朱晋  刘昔猛  徐长城 《长江蔬菜》2014,(18):14-16

为了研究春季栽培时,不同定植密度对甜玉米品种鄂甜玉6号主要农艺性状、产量及品质的影响,对2800、3200、3500、4000株／667m^2 4个种植密度进行了比较试验,结果显示,春季栽培时3200～3500株／667m^2为鄂甜玉6号最适宜的种植密度．其产量较高。  相似文献   

兰州市节能型日光温室结构类型调查及效益分析     

滕汉玮 《长江蔬菜》2014,(4):33-36

通过调查研究,筛选出了4种兰州市较为典型的日光温室结构类型,分别为寿光5代变异Ⅰ型(Q1)、寿光5代变异Ⅱ型(A2)、8.5 m跨度管架拱圆型(H1)、下沉式8.5 m跨度管架拱圆型(H2),理论上温室性能Q1最优,A2次之,H1、H2较差,同时对兰州市日光温室的成本和效益进行了分析。  相似文献