高效液相色谱法测定鸡蛋中氯霉素残留量   总被引：6，自引：0，他引：6  

岳秀英  彭莉  高岚 《四川畜牧兽医》2004,31(3):22-23

采用高效液相色谱法(HPLC)检测鸡蛋中氯霉素的残留量。鸡蛋(全蛋)试样中残留的氯霉素用乙酸乙酯提取,35℃减压蒸干。残渣用正己烷-氯仿(1 1,V/V)溶解,加少量水,混匀后,离心,取上清液作为试样溶液供高效液相色谱仪(紫外检测器)在280nm处测定,外标法定量。本方法在鸡蛋中检出限为2μg/kg,回收率在60％～90％,批内变异系数小于12.0％,批间变异系数小于15.0％。  相似文献   

Effect of lipoic acid on redox regulation,digestive and absorptive function in intestine of mice fed high fat diet     

LI Wu  SHI Yong-hui  YANG Rui-li  YUE Peng  LE Guo-wei 《园艺学报》2009,25(3):577-580

AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8).The control group consumed an ordinary diet.The other two experimental groups were fed with a high fat diet, high fat plus 0.1% LA.After 6 weeks, the activities of digestive enzymes were examined.In order to evaluate the antioxidant status of the mice, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured.To investigate the molecular mechanisms underlying the effects of LA, the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system, accompanied by digestive and absorptive function impairment, was observed in HFD-fed mice.These changes were partially restored in the LA-treated group.DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes, digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD.The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.  相似文献   

两种常温固化木材胶粘剂胶接强度分析     

岳孔  卢晓宁  黄河浪  那斌 《林业科技开发》2007,21(2):54-57

对两种木材用常温固化胶粘剂--单宁-甲醛胶粘剂和聚氨酯胶粘剂胶接的速生杨木的静态剪切强度进行了测试与分析.研究结果表明,以速生杨I-72为被胶接对象,自制的双组分型单宁胶压缩剪切强度范围为8.08～8.92MPa,但木破率较低,要满足日本集成材JAS标准中用胶规定,需进一步研究改进.相比较而言,聚氨酯胶粘剂的胶接强度较高,其压缩剪切强度值均在9.8MPa以上,木破率在75%以上,能够满足日本集成材JAS标准中用胶的规定.  相似文献   

Conversion of the Statistical Combining Ability into a Genetic Concept   总被引：1，自引：0，他引：1  

LV Ai-zhi  ZHANG Hao  ZHANG Zu-xin  TAO Yong-sheng  YUE Bing  ZHENG Yong-lian 《中国农业科学(英文版)》2012,(1):43-52

  相似文献   

青贮玉米育种和栽培的研究与探讨     

岳尧海周小辉  赵万庆任军石贵山  任延辉 《牧草与饲料》2007,1(2):17-18,45

主要对青贮玉米育种、栽培进行了浅析，并指出其发展前景，旨在推动我国青贮玉米育种研究再上一个新台阶。  相似文献   

In Vitro Studies of Cholesterol-lowering Probiotics     

HU Meng-kun  YUE Xi-qing  LI Xiao-ting  GAO Zheng 《农业科技与装备》2008,(2)

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Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis     

ZHANG Shengying  WU Xiaochun  XING Xiaoyong  LIU Jia  YUE Yahui  ZHANG Yangyang  HE Jian  WEN Fengqin  BAO Shijun 《中国畜牧兽医》2007,47(10):3149-3157

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.  相似文献   

基于ArcIMS的江川县森林资源信息发布系统   总被引：2，自引：0，他引：2  

黄彬  岳彩荣  刘涛  黄丽丹 《林业调查规划》2006,31(1):17-20

介绍了ArcIMS的体系结构、特点及技术优势。构建了基于ArcIMS的江川县森林资源信息发布系统,提出了系统设计的思路、功能、特点,并实现了部分功能。  相似文献   

黑加仑嫩枝扦插三个时期的水分管理     

赵晓翠  周自知  岳明哲 《内蒙古林业调查设计》2007,30(5):32-32,57

文章介绍了黑加仑嫩枝扦插苗生根过程中三个时期的水分管理,可供生产实践参考。  相似文献   

霜冻害对苗圃育苗质量的影响分析     

岳增俭  李军 《林业勘查设计》2006,(2):75

分析了苗圃育苗霜冻害产生的原因,提出预防霜冻害的措施。  相似文献