Hepatic endogenous defense potential of propolis after mercury intoxication     

Monika BHADAURIA  Sangeeta SHUKLA  Ramesh MATHUR  Om P. AGRAWAL  Sadhana SHRIVASTAVA  Sonia JOHRI  Deepmala JOSHI  Varsha SINGH  Deepak MITTAL  Satendra Kumar NIRALA 《Integrative zoology》2008,3(4):311-321

Exposure to mercuric chloride (HgCl₂; 5 mg kg^?1 body weight; i.p.) induced oxidative stress in mice and substantially increased lipid peroxidation (LPO) and oxidized glutathione (GSSG) levels, decreased the level of reduced glutathione (GSH) and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum. Therapy with propolis extract, a resinous wax‐like beehive product (200 mg kg^?1 orally, after mercury administration), for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver. Release of serum transaminases, alkaline phosphatase, lactate dehydrogenase and γ‐glutamyl transpeptidase were significantly restored after propolis treatment. The activities of antioxidant enzymes, that is, superoxide dismutase, catalase, glutathione‐S‐transferase and glucose‐6‐phosphate dehydrogenase, were also concomitantly restored towards normal levels after propolis administration. These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury‐induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent.  相似文献   

Vegetative propagation of Dalbergia sissoo: effect of growth regulators, length, position of shoot and type of cuttings on rooting potential in stem cuttings     

Bhupendra SINGH  Rajendra YADAV  B.P. BHATT 《中国林学(英文版)》2012,14(3):187-192

The effect of auxins (IAA or IBA at 100,200,500 mg L 1) on rooting and sprouting parameters differed significantly (p < 0.05).Rooting and sprouting percentages were higher in some treatments while other parameters were significantly higher in other treatments.The results of the present study revealed that rooting was significantly (p < 0.05) affected by the length of cuttings,as well as the position of shoots and type of cuttings.On average,86.0 per cent rooting was observed in the 30-cm long cuttings compared to the other two lengths of cuttings,i.e.,20 and 25 cm,irrespective of any auxin treatment.In a control set,without any auxin treatment,a maximum of 60.0% rooting was recorded in the 30-cm long cuttings and a minimum of 40.0% of the cuttings rooted in the 20-cm long cuttings.Similarly,cuttings collected from the lower and upper portions of shoots resulted in 72.5 and 52.5 per cent rooting respectively,irrespective of auxin treatments.Maximum rooting was observed in the lower portion (40.0%) of cutting compared to the upper portion (30.0%) in the control set without auxin treatment.Leafless branches produced maximum (72.0%) rooting compared to the leafy cuttings (35.0%),irrespective of the auxin treatment.The leafy cuttings produces a minimum of 15.0% sprouting and rooting in the control set.  相似文献   

硫杆菌以及好养异养型硫氧化菌对元素硫的氧化     

YANG Zhi-Hui  K. ST&#;VEN  S. HANEKLAUS  B. R. SINGH  E. SCHNUG 《土壤圈》2010,20(1):71-79

The prediction of the oxidation rate of elemental sulfur (S⁰) is a critical step in sulfur (S) fertilizer strategy to supply plant-available sulfur. An incubation experiment was conducted to determine the rate and amount of S⁰ oxidation in relation to the contribution of Thiobacillus spp. and aerobic heterotrophic S-oxidizing bacteria. After 84 days, 16.3% and 22.4% of the total S⁰ applied to the soil were oxidized at 20 and 30 ℃, respectively. The oxidation of S⁰ proved to be a two-step process with a rapid oxidation during the first 28 days and a slow oxidation from then on. The highest oxidation rate of 12.8 μg S cm^-2 d^-1 was measured during the first two weeks at 30 ℃. At 20 ℃ the highest oxidation rate of 10.2 μg S cm^-2 d^-1 was obtained from two to four weeks after start of the experiment. On an average the soil pH declined by 3.6 and 4.0 units after two weeks of experiment. At the same time the electric conductivity increased nine times. With the oxidation of S⁰ the population of Thiobacillus spp. and aerobic heterotrophic S-oxidizing bacteria increased. The corresponding values for Thiobacillus spp. and aerobic heterotrophic S-oxidizing bacteria increased from 2.9 × 10⁵ and 1.4 × 10⁵ g^-1 soil at the start of the experiment to 4 × 10⁸ and 5.6 × 10⁸ g^-1 soil 14 days after S⁰ application, respectively. No Thiobacillus spp. was present eight weeks after S⁰ application. The results suggested that oxidation of residual S⁰ completely relied on aerobic heterotrophic S-oxidizing bacteria.  相似文献   

Management of root-knot disease of tomato by the application of fly ash in soil   总被引：1，自引：0，他引：1  

M. R. KHAN  M. W. KHAN  & K. SINGH 《Plant pathology》1997,46(1):33-43

The effects of fly ash at different concentrations (0, 10, 20, 30 … 100% vol./vol. in soil) on plant growth and yield were investigated in tomato plants infected or noninfected with root-knot nematode, Meloidogyne incognita (2000 juveniles per plant) in clay pots. An increase in fly ash concentration in the soil correspondingly increased the availability of carbonates, bicarbonates, sulphate, chlorides, B, P, K, Ca, Mg, Mn, Cu and Zn in the soil. The porosity, water-holding capacity, pH, conductivity and cation exchange capacity also increased progressively in the fly ash amended soil. Ash application enhanced plant growth, leaf pigment concentrations, fruit production, weight of fruit/plant and mean fruit weight of both nematode-infected and noninfected tomato plants, being maximum in the soil containing 50 or 60% fly ash. The yield enhancements were 93.6 (infected plants) and 84% (noninfected plants) at 50 and 60% fly ash levels, respectively. Fly ash treatments adversely affected root invasion by juveniles, disease intensity and reproduction of the nematodes. A gradual increase in the ash concentration in soil caused a corresponding decrease in the numbers of invading juveniles, galls and egg masses per root system and eggs/egg mass, being lowest at 100% fly ash i.e. 52, 16, 10 and 81 against 289, 137, 131 and 238 (control), respectively. Linear regression suggested 40% fly ash as the most economic level, enhancing yield of infected plants by 96% and suppressing the nematode disease and reproduction by 63 and 76%, respectively.  相似文献   

Antifungal activity of bergenin, a constituent of Flueggea microcarpa   总被引：1，自引：0，他引：1  

B. PRITHIVIRAJ  U.P. SINGH  M. MANICKAM  J.S. SRIVASTAVA  & A.B. RAY 《Plant pathology》1997,46(2):224-228

The antifungal activity of bergenin against some plant pathogenic fungi, namely, Alternaria alternata , A. brassicae , A. carthami , Fusarium udum , F. oxysporum f.sp. ciceri , Curvularia lunata and Erysiphe pisi , was studied. Bergenin as its monosodium salt was effective against all the fungi and the effective dose for complete inhibition of spore germination varied from 15 μg mL⁻¹ for F. udum to 125 μg mL⁻¹ for E. pisi . Experiments on the effect of bergenin on powdery mildew development under glasshouse conditions revealed that it can control powdery mildew of pea at 2000 μg mL⁻¹ by postinoculation treatment, the results being comparable with those of carbendazim (1000 μg mL⁻¹) and wettable sulfur (2000 μg mL⁻¹). It affected hyphal elongation and the number of primary and secondary branches.  相似文献   

Phytotoxic effects of Parthenium hysterophorus residues on three Brassica species   总被引：3，自引：0，他引：3  

HARMINDER PAL SINGH  DAIZY RANI BATISH  JASVIR KAUR PANDHER  RAVINDER KUMAR KOHLI 《Weed Biology and Management》2005,5(3):105-109

A study was conducted to assess the phytotoxicity of residues of Parthenium hysterophorus , an exotic invasive weed, towards the growth of three Brassica species ( Brassica campestris, Brassica oleracea and Brassica rapa ). The early growth of crops, measured in terms of seedling length and dry weight, was significantly reduced when grown in soil amended with varying amounts of Parthenium residues. A direct relationship was observed between the amount of residue incorporated in the soil and growth reduction. This adverse effect on Brassica crops indicates the presence of some growth-retardatory substances which are possibly released by the residues into the soil medium. In order to test this, aqueous extracts (1, 2, and 4%) of residues were prepared. It was observed that, in a laboratory bioassay, these extracts severely reduced the early growth of Brassica species, thereby indicating the presence of some water-soluble, inhibitory principles in Parthenium residues. A significant amount of the phenolics, the largest group of secondary metabolites usually implicated in allelopathy, was estimated in residue extracts, as well as in residue-incorporated soil. The phenolic content increased with increasing residue concentration, thereby showing their direct involvement in the observed growth inhibitions. Therefore, the study establishes that Parthenium residues exert an allelopathic influence on the early growth of Brassica crops by releasing water-soluble phenolics into the soil.  相似文献   

Colonization and sporulation of Peronospora viciae on cultivars of Pisum sativum     

C. H. DICKINSON  H. SINGH 《Plant pathology》1982,31(4):333-341

The behaviour of Peronospora viciae was examined on four pea ( Pisum sativum ) cultivars (Maro, Superb, Victory Freezer and Surprise) which appeared to exhibit varying degrees of resistance to this pathogen. Penetration into all cultivars was similar and mainly by direct entry through the cuticle. The pathogen grew in a similar manner and at a similar rate in all the cultivars during the early stages (<96 h) of tissue colonization. As it approached the onset of sporulation its growth slowed down in the more resistant cultivars but in no instance were any hypersensitive or other host cell reactions observed. Greater differences between the cultivars were seen in the extent of sporulation, with the least resistant cultivar (Superb) bearing the largest number of sporangiospores. There was much less sporulation on the most resistant cultivar (Maro) and the process was more dependent on temperature than it was on Superb. The development of mature plant resistance occurred in a similar way and on a similar time-scale in all cultivars. The youngest leaflets on plants which were less than 8 weeks old at inoculation showed the least resistance to infection and colonization.  相似文献   

Virome identification in wheat in the Czech Republic using small RNA deep sequencing          下载免费PDF全文

Khushwant SINGH  Jana JARO&#  OVÁ  Jan FOUSEK  CHEN Huan  Jiban Kumar KUNDU 《农业科学学报》2020,19(7):1825-1833

High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA(sRNA) population isolated from plants allows universal virus detection and complete virome reconstruction for a given sample. In the present sRNA deep-sequencing analysis of virus-infected wheat samples in the Czech Republic, samples were firstly tested for barley yellow dwarf viruses(BYDVs), wheat streak mosaic virus(WSMV) and wheat dwarf virus(WDV) using ELISA, RT-PCR and PCR. Subsequent sRNA sequencing of these samples yielded more than ～60 million single-end 50-bp reads with high confidence for nine field samples of wheat. Overall, 16.5% of reads were virus-specific and 83.5% were mapped to the host. More 21-nt reads(～7.7 E+06 reads) were found than 24-nt(～6.20 E+06 reads) or 22-nt(～4.30 E+06 reads) reads. De novo assembly of the high-quality contigs revealed the presence of three earlier reported viruses in the Czech Republic: BYDVs(31.48%), WSMV(24.23%) and WDV(26.66%). We also showed the presence of cereal yellow dwarf virus(14.33%; two species CYDV-RPS and CYDV-RPV(family Luteoviridae/Polerovirus) and wheat yellow dwarf virus(WYDV, 3.30%; Luteoviridae). Phylogenetic analysis showed CYDV and WYDV grouped separately from BYDVs. Furthermore, several recombination breakpoints were found among the groups of yellow dwarf viruses(BYDVs, CYDV, and WYDV). Using RNA deep sequencing, we confirmed the presence of the three known viruses(BYDVs, WSMV, and WDV) and the first record of two species of CYDV and WYDV in wheat in the Czech Republic.  相似文献   

STUDIES ON HUMIC ACIDS OF SEVEN TYPICAL SOILS OF RAJASTHAN     

S. SINGH  G. S. BHANDARI 《European Journal of Soil Science》1965,16(2):183-191

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Changes with time in the potassium content and phyllosilicates in the soil of the Broadbalk continuous wheat experiment at Rothamsted   总被引：1，自引：0，他引：1  

B. SINGH  K. W. T. GOULDING 《European Journal of Soil Science》1997,48(4):651-659

The transformations of phyllosilicates and the changes in potassium (K) balances caused by long-term cropping have been examined in soil from the 153-year-old Broadbalk experiment. Samples taken between 1856 and 1987 and kept in archive were fractionated for particle size and analysed for changes in mineralogy by X-ray diffraction and changes in total K of the coarser and fine fractions. These were compared with K balances made from records of cropping and fertilizer application. No change in mica due to cropping and K-depletion could be detected. Deep ploughing after 1925 mixed chlorite from the subsoil into the surface soil, but this had weathered by 1987. Potassium balances did not agree with measured changes in total K presumably because of unquantified uptake of K from the subsoil and poorly quantified K leaching.  相似文献