PCR detection of the Cryptococcus neoformans CAPS9 gene from a biopsy specimen from a case of feline cryptococcosis.     

R Kano  Y Fujino  N Takamoto  H Tsujimoto  A Hasegawa 《Journal of veterinary diagnostic investigation》2001,13(5):439-442

A polymerase chain reaction assay was developed to detect Cryptococcus neoformans in biopsy samples. The assay detects the CAP59 gene of Cryptococcus neoformans and was used to substantiate cutaneous cryptococcosis in a 5-year-old cat submitted to the Veterinary Medicine Center at the University of Tokyo.  相似文献   

A major gene for low temperature germinability in rice (Oryza sativa L.)     

Kenji Fujino 《Euphytica》2004,136(1):63-68

Low temperature-induced retardation of seedling growth is a common problem in temperate rice-growing areas, at high altitudes of tropical and sub-tropical areas, and in areas with a cold irrigation water supply. Studies on low temperature germinability have revealed complex inheritance of the trait. The purpose of this study was to identify the gene(s) for low temperature germinability using Italica Livorno as a donor parent. The frequency distributions for the germination rate at 15 °C in the F₂ and BC₁F₁ populations showed continuous segregation, suggesting that low temperature germinability was under polygenic control. Since some lines in the BC₁F₁ population showed vigorous low temperature germinability similar to that of Italica Livorno, backcrosses until the BC₅F₁ generation was carried out using Hayamasari as the recurrent parent. Clear segregations of low temperature germinability were observed in the BC₅F₁ and BC₅F₂ populations. The distribution of low temperature germinability fitted a single-gene segregation, indicating that a single dominant gene with a large effect was transferred to Hayamasari. This gene is tentatively symbolized as Ltg(t). Low temperature germinability of near isogenic lines for Ltg(t) was similar to that of Italica Livorno. Ltg(t) should greatly contribute to the improvement and manipulation of low temperature germinability in rice breeding programs. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Genome-wide association mapping focusing on a rice population derived from rice breeding programs in a region     

Kenji Fujino  Mari Obara  Toshiaki Shimizu  Kanako O. Koyanagi  Tomohito Ikegaya 《Breeding Science》2015,65(5):403-410

Plant breeding programs in local regions may generate genetic variations that are desirable to local populations and shape adaptability during the establishment of local populations. To elucidate genetic bases for this process, we proposed a new approach for identifying the genetic bases for the traits improved during rice breeding programs; association mapping focusing on a local population. In the present study, we performed association mapping focusing on a local rice population, consisting of 63 varieties, in Hokkaido, the northernmost region of Japan and one of the northern limits of rice cultivation worldwide. Six and seventeen QTLs were identified for heading date and low temperature germinability, respectively. Of these, 13 were novel QTLs in this population and 10 corresponded to the QTLs previously reported based on QTL mapping. The identification of QTLs for traits in local populations including elite varieties may lead to a better understanding of the genetic bases of elite traits. This is of direct relevance for plant breeding programs in local regions.  相似文献   

Changes in Topographical Features and Soil Properties after Exclosure Combined with Sand-Fixing Measures in Horqin Sandy Land, Northern China   总被引：1，自引：0，他引：1  

Yasuhito Shirato  Tong-Hui Zhang  Toshiya Ohkuro  Hideshi Fujiwara  Ichiro Taniyama 《Soil Science and Plant Nutrition》2005,51(1):61-68

  相似文献   

Applying the Rothamsted Carbon Model for Long-Term Experiments on Japanese Paddy Soils and Modifying It by Simple Tuning of the Decomposition Rate   总被引：2，自引：0，他引：2  

Yasuhito Shirato  Masayuki Yokozawa 《Soil Science and Plant Nutrition》2005,51(3):405-415

  相似文献   

Effects of feeding high volumes of milk replacer on reproductive performance and on concentrations of metabolites and hormones in blood of Japanese black heifer calves     

Yutaka Taguchi  Yudai Inabu  Koki Hayasaki  Noriyuki Maeda  Yoshiro Kanmera  Seiji Yamasaki  Noboru Ota  Kenji Mukawa  Arisa Tsuboi  Hirokuni Miyamoto  Tetsuji Etoh  Yuji Shiotsuka  Ryoichi Fujino  Christopher D. McMahon  Hideyuki Takahashi 《Animal Science Journal》2021,92(1):e13505

We evaluated the effects of feeding high volumes of milk replacer on growth and reproductive performances in Japanese black heifers. Fifty-one heifers were fed milk replacer at 9 L/day for 60 days (9 L × 60 days; n = 18) or 41 days (9 L × 41 days; n = 15), or at 7 L/day for 40 days (7 L × 40 days; n = 18). Artificial insemination (AI) was performed on heifers with ≥270 kg body weight and ≥116 cm body height at 300 days of age. The age at the first AI was 0.35 month later for 7 L × 40 days than the other groups (p < .01). However, age at calving did not differ among treatments (22.1 months). The interval from the first AI to pregnancy tended to be ~2 months longer for the 9 L × 60 days than the other groups (p = .07). Our results showed that feeding high volumes of milk replacer may reduce the age at calving via an improved rate of growth. In addition, we propose that feeding a maximum of 7 L milk replacer for 40 days may be the most appropriate rearing regime because the success of pregnancy per AI may be reduced in calves fed a maximum of 9 L for 41 and 60 days.  相似文献   

Increase in minimal residual disease in peripheral blood before clinical relapse in dogs with lymphoma that achieved complete remission after chemotherapy     

Sato M  Yamazaki J  Goto-Koshino Y  Takahashi M  Fujino Y  Ohno K  Tsujimoto H 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(2):292-296

Background: We developed previously a minimal residual disease (MRD) monitoring system in dogs with lymphoma by exploring a highly sensitive real‐time PCR system. Objectives: To identify the change in MRD before clinical relapse in dogs with lymphoma that achieved complete remission after chemotherapy. Animals: Twenty dogs with multicentric high‐grade B‐cell lymphoma. Methods: MRD levels in peripheral blood mononuclear cells (PBMCs) were measured by real‐time PCR amplifying the rearranged immunoglobulin heavy chain gene. MRD measurement and clinical assessment were performed every 2–4 weeks for 28–601 days after completion of chemotherapy. An increase in MRD was defined as an increase by more than 0.5, calculated by log₁₀[copy number of MRD per 10⁵ PBMCs], based on the uncertainty level observed in a canine lymphoma cell line. Results: During the follow‐up period, 15 dogs relapsed in 28–320 days (median, 120 days) after completion of chemotherapy. An increase in MRD was detected 2 weeks or more before relapse in 14 of the 15 dogs, but an increase in MRD before relapse could not be detected in the remaining 1 dog. The time from increased MRD to clinical relapse was 0–63 days (median, 42 days). In contrast, no increase in MRD was detected in 5 dogs that did not experience clinical relapse. Conclusion and Clinical Importance: An increase in MRD can be detected before clinical relapse in dogs with lymphoma. Application of early reinduction therapy based on an increase in MRD before clinical relapse may improve treatment outcome in canine lymphoma.  相似文献   

Evaluation of cytoreductive efficacy of vincristine, cyclophosphamide, and Doxorubicin in dogs with lymphoma by measuring the number of neoplastic lymphoid cells with real-time polymerase chain reaction     

Sato M  Yamazaki J  Goto-Koshino Y  Takahashi M  Fujino Y  Ohno K  Tsujimoto H 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(2):285-291

Background: The cytoreductive efficacy of the individual components of multidrug chemotherapy for canine lymphoma is difficult to evaluate after complete remission. Objectives: To compare the cytoreductive efficacy of vincristine (VCR), cyclophosphamide (CPA), and doxorubicin (DXR) in dogs that received a 6‐month modified version of the University of Wisconsin‐Madison chemotherapy protocol (UW‐25). Animals: Twenty‐nine dogs with high‐grade B‐cell multicentric lymphoma. Methods: Rearranged immunoglobulin heavy chain gene fragments from lymphoma cells were amplified by polymerase chain reaction (PCR) and sequenced to prepare clone‐specific primers and probes for real‐time PCR. The number of lymphoma cells in peripheral blood was measured from diagnosis to week 11 of UW‐25. Results: The number of lymphoma cells after the 1st administration of VCR, CPA, and DXR in weeks 1–4 was decreased in 29/29 (100%), 15/29 (51.7%), and 26/27 (96.3%) dogs, respectively. The cytoreductive efficacy of CPA was less than that of VCR and DXR. VCR, CPA, and DXR administered in weeks 6–9 were effective in 5/26 (19.2%), 5/20 (25.0%), and 14/19 (73.7%) dogs, respectively, indicating the sustained cytoreductive efficacy of DXR. CPA nonresponders were heavier and exhibited a shorter 1st remission than CPA responders. Conclusion and Clinical Importance: When using UW‐25 for treatment of canine lymphoma, CPA was found to have less cytoreductive efficacy than VCR and DXR. Real‐time PCR‐based quantification of tumor cells is an objective marker of the efficacy of chemotherapeutic agents.  相似文献   

Real-time ultrasonographic evaluation of canine gastric motility in the postprandial state     

Tsukamoto A  Ohno K  Tsukagoshi T  Maeda S  Nakashima K  Fukushima K  Fujino Y  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1133-1138

Gastric motility is affected by several pathological conditions which may induce upper gastrointestinal clinical symptoms. The pathogenesis of canine gastric motility disorders is poorly understood because of methodological limitations. This study aimed at establishing a simple method for evaluating postprandial gastric motility in dogs. Gastric motility was ultrasonographically assessed in 7 healthy beagles using a technique previously described in humans. The motility index (MI), an indicator of gastric antral motility, was calculated by measuring the area of the gastric antrum in both a contracted and relaxed phase and by counting the number of contractions. The MI was measured every 30 min for 3 hr after feeding and compared with gastric emptying as assessed by a (13)C-octanoic acid breath test. The MI at 30 min had the lowest variability in the 7 dogs (mean SD, 9.77 ± 0.42; coefficient of variance, 4.25%), and a significant correlation was observed with gastric emptying coefficient (R(2)=0.8126, P=0.005) and half-emptying time (R(2)=0.654, P=0.027). When atropine was administered, a significant decrease in the MI at 30 min was observed compared with the control (9.77 ± 0.42 vs. 5.19 ± 0.22, P=0.0003). In conclusion, evaluation of the MI at 30 min is suitable for assessing gastric motility and enables us to assess gastric motility simply in a short time. By using this method, further studies for the pathogenesis of canine gastric motility disorders are warranted.  相似文献   

Testing the suitability of the Rothamsted Carbon model for long-term experiments on Japanese non-volcanic upland soils     

Yasuhito Shirato  Ichiro Taniyama 《Soil Science and Plant Nutrition》2013,59(6):921-925

We evaluated the suitability of the Rothamsted Carbon (RothC) model for long-term experiments on Japanese non-volcanic upland soils using 6 long-term experimental data sets: 2 Brown Lowland Soils, 2 Yellow Soils, 1 Gray Lowland Soil, and 1 Brown Forest Soil. The predicted changes in the content of soil carbon with time were very close to the observed values in almost all the treatments at all the 6 sites. These 6 sites were distributed from North to South across Japan and included a variety of climatic conditions, soil textures, and land management practices. We therefore concluded that the RothC model adequately simulated changes in the soil carbon content with time in Japanese non-volcanic upland soils.  相似文献