Properties of scallop mantle collagen: its content,tissue distribution and thermal behavior     

Shoshi Mizuta  Yuko Nakanishi  Masashi Shiraishi  Yoshihiro Yokoyama  Reiji Yoshinaka 《Fisheries Science》2007,73(6):1353-1361

ABSTRACT:   To obtain fundamental information for the effective use of scallop Patinopecten yessoensis mantle, which is one of the underutilized marine resources. Some properties of collagen contained in the mantle were examined by chemical and histochemical techniques. Collagen content in the mantle varied annually, ranging from 0.98 to 1.72% of wet tissue, 7.7 to 12.6% of dry tissue and 13.5 to 26.5% of total protein, being relatively in high level of collagen content of invertebrate muscles. Collagen fiber was densely distributed in the inner connective tissue matrix of the mantle pallial, in contrast to the inner fold part which was rich in muscle fibers. The collagen contained in the crude collagen fraction (residue after alkali extraction), prepared from the mantle, was revealed to have considerably low solubility on hot-water extraction, constantly less than 20% of the total collagen at the temperatures in the range of 20–90°C.  相似文献   

Comparative Investigation of Body Composition in Male Dogs Using CT and Body Fat Analysis Software     

Toyokazu KOBAYASHI  Hiroshi KOIE  Akiko KUSUMI  Masato KITAGAWA  Kiichi KANAYAMA  Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):439-446

  相似文献   

Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan     

Satoshi Haga  Hiroshi Ishizaki  Miwa Nakano  Seiji Nakao  Kiyoshi Hirano  Yoshito Yamamoto  Miya Kitagawa  Hiroyuki Sasaki  Yoshihiro Kariya 《Animal Science Journal》2014,85(2):135-142

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.  相似文献   

Comparative sensitivities of several dot blot methods used to detect bacterial kidney diseases     

M. SAKAI  N. AMAAKI  S. ATSUTA  M. KOBAYASHI 《Journal of fish diseases》1987,10(3):229-231

  相似文献   

Enhancement of resistance to vibriosis in rainbow trout, Oncorhynchus mykiss (Walbaum), by oral administration of Clostridium butyricum bacterin   总被引：1，自引：0，他引：1  

M. SAKAI  T. YOSHIDA  S. ATSUTA  M. KOBAYASHI 《Journal of fish diseases》1995,18(2):187-190

  相似文献   

Factors that limit maintenance of recombinant rumen bacterium in sheep rumen     

Yasuo KOBAYASHI  Mayu YAMAMOTO 《Animal Science Journal》2002,73(2):131-136

Factors limiting the maintenance of recombinant ruminal bacterium in the rumen were evaluated in vitro , using batch culture prepared from rumen fluid of sheep. Butyrivibrio fibrisolvens expressing a foreign xylanase gene ( B. fibrisolvens NO4) was used as a tested recombinant that was selectable on an erythromycin-containing agar medium. The recombinant tended to reduce its level slowly in the rumen fluid of sheep on a high hay diet, while its initial decrease was more apparent in the rumen fluid of sheep on a high concentrate diet. Incubation with cell-free ruminal fluid revealed a significant decrease of inoculated recombinant, suggesting the presence of antibacterial factors limiting maintenance of the recombinant. In particular, during the first 12 h of incubation this inhibition was more notable in culture prepared from rumen fluid of sheep given the high concentrate diet. Autoclaving the cell-free rumen fluid inactivated the inhibition. Numbers of the recombinant for inoculation did not influence the final level of survived recombinant, that is, the initial depression was larger as more recombinant was inoculated. Subculturing with xylan before inoculation and/or direct addition of xylan to the batch culture did not improve survival of the recombinant. From these results it is suggested that the level of survived recombinant is limited to 10^2–4/mL of in vitro batch culture with restricted energy supply and that initial depression of the recombinant is mainly caused by the heat-sensitive antibacterial factors not associating with microbial cells in the rumen.  相似文献   

Tissue-dependent somaclonal mutation frequencies and spectra enhanced by ion beam irradiation in chrysanthemum     

Masachika Okamura  Yoshihiro Hase  Yoshiya Furusawa  Atsushi Tanaka 《Euphytica》2015,202(3):333-343

  相似文献   

Effects of Organo-mineral Complexes on Flocculation, Settlement and Vertical Distribution of Bioelements in Soil Suspensions     

Atsushi Suetsugu  Takashi Sato  Yoshihiro Kaneta  Atsushi Sato 《Soil Science and Plant Nutrition》2005,51(3):323-331

  相似文献   

Applicability of Aegilops tauschii drought tolerance traits to breeding of hexaploid wheat     

Quahir Sohail  Tomoe Inoue  Hiroyuki Tanaka  Amin Elsadig Eltayeb  Yoshihiro Matsuoka  Hisashi Tsujimoto 《Breeding Science》2011,61(4):347-357

Few genes are available to develop drought-tolerant bread wheat (Triticum aestivum L.) cultivars. One way to enhance bread wheat’s genetic diversity would be to take advantage of the diversity of wild species by creating synthetic hexaploid wheat (SW) with the genomic constitution of bread wheat. In this study, we compared the expression of traits encoded at different ploidy levels and evaluated the applicability of Aegilops tauschii drought-related traits using 33 Ae. tauschii accessions along with their corresponding SW lines under well-watered and drought conditions. We found wide variation in Ae. tauschii, and even wider variation in the SW lines. Some SW lines were more drought-tolerant than the standard cultivar Cham 6. Aegilops tauschii from some regions gave better performing SW lines. The traits of Ae. tauschii were not significantly correlated with their corresponding SW lines, indicating that the traits expressed in wild diploid relatives of wheat may not predict the traits that will be expressed in SW lines derived from them. We suggest that, regardless of the adaptability and performance of the Ae. tauschii under drought, production of SW could probably result in genotypes with enhanced trait expression due to gene interactions, and that the traits of the synthetic should be evaluated in hexaploid level.  相似文献   

Regulation of muscular glutamate metabolism by high‐protein diet in broiler chicks     

Hiroyuki KOBAYASHI  Atsushi EGUCHI  Wataru TAKANO  Masahiro SHIBATA  Motoni KADOWAKI  Shinobu FUJIMURA 《Animal Science Journal》2011,82(1):86-92

The major taste active component, glutamate (Glu), improves the taste of meat. In this study, we investigated the effect of a short‐term high‐protein (HCP) diet on the intramuscular free Glu content to improve the taste of meat. Furthermore, we elucidated how the muscle free Glu content was controlled by the HCP diet. Chicks (14 days old) were fed the control diet or HCP diet for 10 days. Plasma and muscle free amino acid concentrations, and activity and messenger RNA (mRNA) expression of muscle enzymes related to Glu metabolism were determined. Muscle free Glu content was increased (P < 0.01) by 51%. Activity and mRNA expression of glutaminase (GA), which is one of the major Glu‐related enzymes, were significantly decreased (P < 0.05) in the HCP group because of feedback inhibition. The mRNA expression of lysine α‐ketoglutarate reductase (LKR), which is the enzyme involved in lysine (Lys) degradation and Glu production, was significantly increased (P < 0.001) in the HCP group. These results suggest that short‐term dietary HCP feeding is an effective treatment for improving the taste of meat. Furthermore, our results suggest that the free Glu content in muscle is regulated by GA and LKR.  相似文献