Superoxide Dismutase Activity in the Oviductal and Uterine Fluid of the Bitch and the Effects of the Enzyme on Viability,Motility and Hyperactivation of Canine Sperm In Vitro     

Masanori KOBAYASHI  Miho WADA  Tatsuya HORI  Eiichi KAWAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):741-743

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Imatinib Responsiveness in Canine Mast Cell Tumors Carrying Novel Mutations of c-KIT Exon 11     

Yuko NAKANO  Tetsuya KOBAYASHI  Fukiko OSHIMA  Eri FUKAZAWA  Tetsushi YAMAGAMI  Yozo SHIRAISHI  Masamine TAKANOSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):545-548

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Construction of a linkage map based on retrotransposon insertion polymorphisms in sweetpotato via high-throughput sequencing     

Yuki Monden  Takuya Hara  Yoshihiro Okada  Osamu Jahana  Akira Kobayashi  Hiroaki Tabuchi  Shoko Onaga  Makoto Tahara 《Breeding Science》2015,65(2):145-153

Sweetpotato (Ipomoea batatas L.) is an outcrossing hexaploid species with a large number of chromosomes (2n = 6x = 90). Although sweetpotato is one of the world’s most important crops, genetic analysis of the species has been hindered by its genetic complexity combined with the lack of a whole genome sequence. In the present study, we constructed a genetic linkage map based on retrotransposon insertion polymorphisms using a mapping population derived from a cross between ‘Purple Sweet Lord’ (PSL) and ‘90IDN-47’ cultivars. High-throughput sequencing and subsequent data analyses identified many Rtsp-1 retrotransposon insertion sites, and their allele dosages (simplex, duplex, triplex, or double-simplex) were determined based on segregation ratios in the mapping population. Using a pseudo-testcross strategy, 43 and 47 linkage groups were generated for PSL and 90IDN-47, respectively. Interestingly, most of these insertions (~90%) were present in a simplex manner, indicating their utility for linkage map construction in polyploid species. Additionally, our approach led to savings of time and labor for genotyping. Although the number of markers herein was insufficient for map-based cloning, our trial analysis exhibited the utility of retrotransposon-based markers for linkage map construction in sweetpotato.  相似文献   

Presence of a Temperature Gradient Among Genital Tract Portions and the Thermal Changes Within These Portions Over the Estrous Cycle in Beef Cows     

Hossam EL-SHEIKH ALI  Go KITAHARA  Youji TAMURA  Ikuo KOBAYASHI  Koichiro HEMMI  Shidow TORISU  Hiroshi SAMESHIMA  Yoichiro HORII  Samy ZAABEL  Shunichi KAMIMURA 《The Journal of reproduction and development》2013,59(1):59-65

The aim of the present study was to describe the temperature of the different portions of the female genital tract and their relation to rectal temperature and to investigate the effect of steroid hormones profiles on these variables over the estrous cycle in cattle. Four nonpregnant Japanese Black cows were investigated daily over two successive estrous cycles using a digital thermometer with a long probe and rounded-end sensor to record the temperature of the rectum (RT), vagina (VT), cervix (CT), uterine body (UBT) and uterine horns (UHT). Blood samples were collected immediately before temperature recording to assay peripheral levels of progesterone (P₄) and estradiol-17β (E₂). Moreover, transrectal ultrasonography was carried out after temperature recording to monitor the ovulatory follicle and track ovulation. During the experiment, the ambient temperature and relative humidity were recorded for further calculation of the temperature humidity index (THI). The temperature within the genital tracts in these cows progressively increased towards the uterine horns from the vagina. The VT, CT, UBT and UHTs were significantly higher in association with peripheral P₄ concentrations greater than 4 ng/ml (mid-luteal phase) when compared with lower peripheral P₄ concentrations. The VT was more significantly (P<0.01) correlated to the CT, UBT and UHTs than RT. In conclusion, a temperature gradient was present among the vagina, cervix and uterus over the estrous cycle, and changes in peripheral P₄ concentrations were associated with the thermal variations within these portions. The VT could be more beneficial than RT in monitoring temperature of deeper portions of the female genital tract in bovine.  相似文献   

Electrophysiological and Morphological Evidence for Synchronized GnRH Pulse Generator Activity Among Kisspeptin/Neurokinin B/Dynorphin A (KNDy) Neurons in Goats     

Yoshihiro WAKABAYASHI  Takashi YAMAMURA  Kohei SAKAMOTO  Yuji MORI  Hiroaki OKAMURA 《The Journal of reproduction and development》2013,59(1):40-48

Neurons in the arcuate nucleus (ARC) that concomitantly express kisspeptin, neurokinin B (NKB) and dynorphin A are termed KNDy neurons and are likely candidates for the intrinsic gonadotropin-releasing hormone (GnRH) pulse generator. Our hypothesis is that KNDy neurons are functionally and anatomically interconnected to generate discrete neural signals that govern pulsatile GnRH secretion. Our goal was to address this hypothesis using electrophysiological and anatomical experiments in goats. Bilateral electrodes targeting KNDy neurons were implanted into ovariectomized goats, and GnRH pulse generator activity, represented by characteristic increases in multiple-unit activity (MUA volleys), was measured. Spontaneous and pheromone- or senktide (an NKB receptor agonist)-induced MUA volleys were simultaneously recorded from both sides of the ARC. An anterograde tracer, biotinylated dextran amine (BDA), was also injected unilaterally into the ARC of castrated male goats, and the distribution of fibers containing both BDA and NKB was examined using dual-labeling histochemistry. The results showed that MUA volleys, regardless of origin (spontaneous or experimentally induced), occur simultaneously between the right and left sides of the ARC. Tract tracing indicated that axons projecting from NKB neurons in the ARC were directly apposed to other NKB neuronal cells located bilaterally in the ARC. These results demonstrate that GnRH pulse generator activity occurs synchronously between both sides of the ARC in goats and that KNDy neurons are bilaterally interconnected in the ARC via NKB-containing fibers. Taken together, the results suggest that KNDy neurons form a neuronal circuit to synchronize burst activity among KNDy neurons and thereby generate discrete neural signals that govern pulsatile GnRH secretion.  相似文献   

Translation of continuous artificial selection on phenotype into genotype during rice breeding programs     

Kenji Fujino  Yoshihiro Kawahara  Kanako O. Koyanagi  Kenta Shirasawa 《Breeding Science》2021,71(2):125

Understanding genetic diversity among local populations is a primary goal of modern crop breeding programs. Here, we demonstrated the genetic relationships of rice varieties in Hokkaido, Japan, one of the northern limits of rice cultivation around the world. Furthermore, artificial selection during rice breeding programs has been characterized using genome sequences. We utilized 8,565 single nucleotide polymorphisms and insertion/deletion markers distributed across the genome in genotype-by-sequencing for genetic diversity analyses. Phylogenetics, genetic population structure, and principal component analysis showed that a total of 110 varieties were classified into four distinct clusters according to different populations geographically and historically. Furthermore, the genome sequences of 19 rice varieties along with historic representations in Hokkaido, nucleotide diversity and F_ST values in each cluster revealed that artificial selection of elite phenotypes focused on chromosomal regions. These results clearly demonstrated the history of the selections on agronomic traits as genome sequences among current rice varieties from Hokkaido.  相似文献   

Ingestion of proteoglycan fraction from shark cartilage increases serum inhibitory activity against matrix metalloproteinase-9 and suppresses development of N-nitrosobis(2-oxopropyl)amine-induced pancreatic duct carcinogenesis in hamster     

Kitahashi T  Ikawa S  Sakamoto A  Nomura Y  Tsujiuchi T  Shimizu K  Sasabe S  Park EY  Nakamura Y  Tsutsumi M  Sato K 《Journal of agricultural and food chemistry》2012,60(4):940-945

A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster.  相似文献   

Foot rot disease of sweet potato in Japan caused by Diaporthe destruens: first report,pathogenicity and taxonomy     

Maeda  Ai  Minoshima  Ayaka  Kawano  Shinji  Nakamura  Misa  Takushi  Tetsuya  Yamashiro  Maki  Kawamura  Futoshi  Oshiro  Atsushi  Ichinose  Katsuya  Okada  Yoshihiro  Rossman  Amy Y.  Hirooka  Yuuri 《Journal of General Plant Pathology》2022,88(1):33-40

Journal of General Plant Pathology - Foot rot, also known as stem rot or tuber-root rot syndrome, on sweet potato occurs in sweet potato fields in Okinawa, Japan. Isolates of one fungal species...  相似文献   

Cholinergic substance in the caudal neurosecretory storage organ of fish     

KOBAYASHI H  UEMURA H  OOTA Y  ISHII S 《Science (New York, N.Y.)》1963,141(3582):714-716

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Viral-derived DNA invasion and individual variation in an Indonesian population of large flying fox Pteropus vampyrus     

Atsuo IIDA  Hitoshi TAKEMAE  Ronald TARIGAN  Ryosuke KOBAYASHI  Hirokazu KATO  Hiroshi SHIMODA  Tsutomu OMATSU  Supratikno  Chaerul BASRI  Ni Luh Putu Ika MAYASARI  Srihadi AGUNGPRIYONO  Ken MAEDA  Tetsuya MIZUTANI  Eiichi HONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1068

Here, we performed next-generation sequencing (NGS) on six large flying foxes (Pteropus vampyrus) collected in Indonesia. Seventy-five virus species in the liver tissue of each specimen were listed. Viral homologous sequences in the bat genome were identified from the listed viruses. This finding provides collateral evidence of viral endogenization into the host genome. We found that two of the six specimens bore partial sequences that were homologous to the plant pathogens Geminiviridae and Luteoviridae. These sequences were absent in the P. vampyrus chromosomal sequences. Hence, plant viral homologous sequences were localized to the hepatocytes as extrachromosomal DNA fragments. Therefore, this suggests that the bat is a potential carrier or vector of plant viruses. The present investigation on wild animals offered novel perspectives on viral invasion, variation, and host interaction.  相似文献