Estimation of planting date in paddy fields by time-series MODIS data for basin-scale rice production modeling     

Tsujimoto  Kumiko  Ohta  Tetsu  Hirooka  Yoshihiro  Homma  Koki 《Paddy and Water Environment》2019,17(2):83-90

Paddy and Water Environment - Projection of future changes in agricultural water demand and supply through numerical model simulations can be one of the reference materials used for long-term rural...  相似文献   

Structural characterization of carangid fish myoglobins     

Hasan MM  Watabe S  Ochiai Y 《Fish physiology and biochemistry》2012,38(5):1311-1322

The primary structures of myoglobin (Mb) from the following five carangid species were determined: yellowtail Seriola quinqueradiata, greater amberjack Seriola dumerili, yellowtail kingfish Seriola lalandi, Japanese horse mackerel Trachurus japonicus, and silver trevally Pseudocaranx dentex. The sequences were of varying composition both in the coding and in the noncoding regions, but all contained the open reading frame of 444 nucleotides encoding 147 amino acids. Amino acid sequence identities of carangid Mbs were in the range of 81-99%. The similarity of the heme pocket and associated heme-binding residues of carangid Mbs were evidence of the conservative nature of Mbs. Similar to the other teleost Mbs, carangid Mbs did not contain a D helix and had mostly conserved A and E helices as well as E-F and G-H inter-helical segments. Hydropathy profiles of carangid Mbs showed species-specific variations where silver trevally Mb exhibited generally higher hydrophobicity. Phylogenetic analysis based on the primary structures was in agreement with conventional morphological taxonomy, establishing close proximity of carangid Mbs with those of cichlid and scombroid, the other members of the Perciformes order.  相似文献   

Ecological studies on subtropical evergreen broad-leaved forest in Okinawa, Japan: Litter production and nutrient input   总被引：1，自引：1，他引：0  

Xiaoniu Xu  Yoshihiro Tokashiki  Eiji Hirata  Tsutomu Enoki  Kangoro Nogami 《Journal of Forest Research》2000,5(3):151-156

Patterns of litterfall and nutrient input in a subtropical evergreen broad-leaved forest in northern Okinawa, Japan, were studied during May, 1996–April, 1999. The mean annual rate of litterfall in the five sampling plots ranged from 6.84 to 8.93 Mg ha⁻¹ yr⁻¹, of which 63.3–68.5% were leaves; 22.4–29.1% woody parts (including branches < 5.0 cm in diameter and bark); 2.8–5.0% sexual organs and 4.6–6.3% miscellaneous material. Significant differences were found among plots and among years. Significantly monthly differences pronounced seasonal patterns in litterfall were observed. Total litterfall and leaf litter showed negative correlations with relative basal area of the dominant species,Castanopsis sieboldii; and showed positive correlations with mean height of the stands. The dominant species,C. sieboldii produced an average of 2.36 Mg ha⁻¹ yr⁻¹ of leaf litter, which covered 30.5% of the annual litter production, and the nutrient input from those litterfall contributed 32.3, 28.3, 30.2, 22.2, 32.5, and 30.5% of total N, P, K, Ca, Mg, and Na, respectively. Nutrient use efficiency in litter production was high, especially for P and K compared with other broad-leaved forests in Japan indicating that P and K may be limiting in Okinawan evergreen broad-leaved forest.  相似文献   

Existence of a 400 kDa glycoprotein in the dermis of sea cucumber Apostichopus armata: partial purification and characterization     

Shoshi Mizuta  Yuuki Koizumi  Shiori Inoue  Chiaki Someya  Masatomi Hosoi  Yoshihiro Yokoyama  Reiji Yoshinaka 《Fisheries Science》2013,79(5):833-839

A large size (400 kDa) non-collagenous protein was detected as a major component in the extract, with neutral salt solution, from the dermis of sea cucumber Apostichopus armata. On SDS-PAGE analysis, the 400 K component shifted to a lower molecular weight component (about 200 K) by reduction with 2-mercaptoethanol, and they were both reactive for periodic acid-Schiff (PAS) reaction staining. From these results, this protein was suggested to be a glycoprotein consisting of disulfide-bonded two subunits with almost equal molecular weight (200 K). In addition to relatively high contents (>100/1,000 residues) of aspartic and glutamic acids, cysteine was also detected (6.1/1,000 residues) in amino acid analyses of this protein partially purified by anion-exchange column chromatography. These combined results suggest the structural similarity of the 400 K component to fibronectins from other vertebrate and invertebrate animals.  相似文献   

Monitoring fungal viability and development in plants infected with <Emphasis Type="Italic">Colletotrichum higginsianum</Emphasis> by quantitative reverse transcription-polymerase chain reaction     

Mari Narusaka  Tomonori Shiraishi  Masaki Iwabuchi  Yoshihiro Narusaka 《Journal of General Plant Pathology》2010,76(1):1-6

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Protective effect of Pasteurella multocida cell-free antigen and toxoid against challenge with toxigenic strains of pasteurella multocida in mice     

Uchida C  Kimura Y  Kubota S  Sasaki O 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):737-740

The cell-free antigen (CFA) obtained from the culture supernatant of Pasteurella multocida (P. multocida) and the toxin (PMT) purified from CFA were inactivated and mixed with oil adjuvant to prepare a trial vaccine. Both of the mice immunized with CFA and PMT toxoid vaccine were noticeably protected against intratracheal challenge with toxigenic strains of P. multocida. Nevertheless, the protective indices of the mice immunized with CFA vaccine indicate that it is more protective and clears away the bacteria more promptly than in the mice immunized with PMT vaccine. The results suggested that CFA would possibly be good as an effective antigen to toxigenic strains of P. multocida infection.  相似文献   

Estrogen receptor alpha expression in the medial preoptic area and the medial basal hypothalamus under different physiological conditions in cattle     

Tanaka T  Shiina T  Hayashi S  Okamura H  Kamomae H  Kaneda Y 《The Journal of reproduction and development》2003,49(1):55-60

The distribution and morphology of immunoreactive estrogen receptor alpha (ERalpha)- containing cells were examined in the cattle brain. The brains were collected from two cows and a male calf. One of the cows was sacrificed one week after parturition. ERalpha expressions in the preoptic area of the rostral forebrain and the medial basal hypothalamic area were evaluated immunohistochemically using a mouse monoclonal antibody. In all animals, the signals that indicate ERalpha were detected in the medial preoptic area, the level of the organum vasculosum of the lamina terminalis and the bed nucleus of the stria terminalis. In the caudal hypothalamic region, they were detected in the arcuate nucleus, the periventricular and ventromedial hypothalamic nuclei. ERalpha-containing cells were characterized by a dense nuclear reaction product. In all the subjects examined, ERalpha signals were clearly detected in the cytoplasm as well. The density of ERalpha expression was different among the three cattle; the number of ERalpha containing cells in the postpartum cow was much lower than that in the other cow and the male calf. The present findings suggest that ERalpha expression in the forebrain is influenced by the reproductive status in the cattle.  相似文献   

Pathological and serological evidence of Brucella-infection in baleen whales (Mysticeti) in the western North Pacific     

Ohishi K  Zenitani R  Bando T  Goto Y  Uchida K  Maruyama T  Yamamoto S  Miyazaki N  Fujise Y 《Comparative immunology, microbiology and infectious diseases》2003,26(2):125-136

Abnormal testes and uterus were observed in 13 males (33%) and one female (3%) out of 40 common minke whales (Balaenoptera acutorostrata) in the western North Pacific. Similar lesions were found in testis and ovary, respectively, in one male (2%) and female (2%) out of 43 Bryde's whales (Balaenoptera edeni) in the western North Pacific. Grossly, granular lesions with caseation and calcification were main pathological signs, and they were restricted to reproductive organs of mature whales. Chronic purulent or granulomatous orchitis was observed by microscopic analysis. Antibodies to Brucella species were detected in the serum samples of 15/40 (38%) of common minke whales and 4/43 (9%) of Bryde's whales. Neither pathological nor serological change was found in the examined sperm whales (Physeter macrocephalus) in the western North Pacific and Antarctic minke whales (Balaenoptera bonaerensis). These results strongly suggest that Brucella infection was involved in two species of baleen whales (Mysticeti) in the North Pacific.  相似文献   

Incidence of Salmonella infection in healthy dogs in Gifu Prefecture,Japan     

Fukata T  Naito F  Yoshida N  Yamaguchi T  Mizumura Y  Hirai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1079-1080

A total of 1,013 feces samples and 8 mesenteric lymphonodus samples obtained from apparently healthy dogs were examined for the incidence of salmonella infection. One strain of S. typhimurium (ST) was isolated from feces of one dog, and S. enteritidis (SE) was isolated from the mesenteric lymphonodus of one dog. Sera obtained from 330 apparently healthy dogs were examined for Salmonella antibodies using an ELISA with heated whole cells of SE and ST. Fifty-one of the 330 serum samples were considered to be positive for salmonella antibodies, including 12 which were SE-positive and 39 which were ST-positive. These results indicate that dogs cause possible environmental problems as Salmonella carriers.  相似文献   

Gene expression profiles of CD4/CD8 double‐positive T cells in porcine peripheral blood          下载免费PDF全文

Mie Okutani  Takamitsu Tsukahara  Yoshihiro Kato  Kikuto Fukuta  Ryo Inoue 《Animal Science Journal》2018,89(7):979-987

A characteristic subset of T cells, known as double positive T cells (DPTC) and expressing both cluster of differentiation 4 (CD4) and CD8, is observed in porcine peripheral blood. Previous studies suggested that DPTC might be memory cells. However, detailed phenotypes and functions of DPTC are yet to be fully elucidated and thus, the relatedness of DPTC with memory phenotypes remains unclear. In this study, DPTC gene expression profiles in peripheral blood were analyzed by DNA microarray in Experiment 1 and compared with those of CD4 single positive T cells (4SPTC) and CD8 single positive T cells (8SPTC). Expressions of IFNG, CCL5, NCK2, CCR2 and ITGB1 were higher than that of 4SPTC and 8SPTC. In contrast, expressions of CCR7 and SELL were lower than that of 4SPTC and 8SPTC. These results suggested that DPTC were either effector T cells or effector memory T cells (T_EM). Next, to determine whether DPTC were effector T cells or T_EM, differences in the response of DPTC and 8SPTC against immunized/primed antigens were compared (Experiment 2). While DPTC showed quick elevation of IL2 and CD25 gene expressions against in vitro stimulation of primed/immunized antigens, 8SPTC did not. These results suggest that at least some DPTC likely belong to T_EM.  相似文献