Immunomodulatory effects of heated ovomucoid-depleted egg white in a BALB/c mouse model of egg allergy     

Jiménez-Saiz R  Rupa P  Mine Y 《Journal of agricultural and food chemistry》2011,59(24):13195-13202

Oral immunotherapy (OIT) is a promising therapeutic approach for treating food allergy. The treatment with heated ovomucoid-depleted egg white (HOMEW) in egg-allergic patients is noteworthy; however, OIT protocols are still experimental, and a better knowledge of the underlying mechanism is required. The objective of this work was to investigate the immunomodulatory effects of HOMEW and characterize the underlying mechanism in a BALB/c mouse model of egg allergy. Mice were sensitized with EW and treated with HOMEW. Post treatment, mice were challenged with EW and euthanized for collecting blood and spleen. Markers of allergic clinical outcomes were measured as histamine concentration, serum antibody activity, and cytokine production from cultured splenocytes. Digestibility of HOMEW was assessed mimicking physiological conditions in vitro. The HOMEW demonstrated high digestibility. The treatment induced a marked increase of the Th1/Th2 ratio in the high-dose treatment group. Treated mice had significantly less histamine, EW-specific IgE, and IL-4 and more IFN-γ and IL-10. This study confirms the mechanisms involved in successful tolerance induction with OIT using HOMEW and allows understanding of the vital role of surrogate allergy markers involved in immune modulation.  相似文献   

Screening of therapeutic targets for canine mast cell tumors from a variety of kinase molecules     

Takeuchi Y  Fujino Y  Watanabe M  Nakagawa T  Ohno K  Sasaki N  Sugano S  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1295-1302

Options of systemic treatment for canine MCT have been still limited and most canine cases with MCTs eventually undergo relapses even after achievement of a remission. Thus additional therapies are required to establish for the tumor. To identify the novel candidate therapeutic targets for canine MCT, the mRNA expression and phosphorylation statuses of several receptor or non-receptor kinases as well as the inhibitory effect of 95 specific inhibitors on the growth were assessed in three canine MCT cell lines (HRMC, VIMC1 and CMMC1). Among the 14 targets, the mRNAs of 11, 7 and 7 kinases were amplified in HRMC, VIMC1 and CMMC1, respectively. The mRNAs of VEGFR3, PDGFRα, SRC, YES, LCK and FYN were detected in all cell lines. The phosphorylation of 12, 8 and 7 kinases was observed by using specific antibody arrays in HRMC, VIMC1 and CMMC1, respectively. DTK, EPHB6, AMPKα1, CREB, STAT5a and STAT5b were phosphorylated in all cell lines. The 10, 9 and 17 inhibitors exhibited the biological activity against the growth of HRMC, VIMC1 and CMMC1, respectively. Only three inhibitors such as SB218078 (for Chk1), PDGF RTK inhibitor IV (for PDGFR) and radicicol (for Hsp90) suppressed the growth of all three cell lines. The present study indicated that several kinases, such as Chk1, PDGFR and Hsp90, could be used as therapeutic targets in the treatment for canine MCT. Further studies and clinical trials are warranted to apply the inhibitors for the treatment of the tumor.  相似文献   

Effect of ovary lipid from Skipjack tuna (Katsuwonus pelamis) on brain monoamines in rats     

Ookawa K  Mochizuki K  Shida E  Suzuki T  Suzuki T  Ooba T  Matumoto T  Hokari Y  Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):593-598

In our previous experiments with rats, ovary lipid from Skipjack Tuna (Katsuwonus pelamis) (OLS) was shown to have a mitigating effect on anxiety and/or fear in elevated T-maze tests. This suggests that OLS has some effect on the central nervous system (CNS) of rats. Thus, we performed experiments to examine the status of CNS in rats given OLS. The effect of OLS on chronic stress was also examined at the same time. The feed for control rats used oil and fat that have the same energy percentages for n-6 and n-3 fatty acids and the same n-6/n-3 ratio as OLS. As a result, rats given OLS for 28 days had lower serotonin levels in various brain areas regardless of stress application, showing that OLS affected the serotonin nervous system. From this, it was inferred that the ability of OLS to mitigate anxiety and/or fear resulted from its action on CNS, especially the serotonin nervous system. Substances other than the essential fatty acids may have been responsible for the action of OLS on monoamines and the metabolites. The effect of OLS on CNS, especially the serotonin nervous system, suggests that OLS may suppress anxiety.  相似文献   

Active expression of matrix metalloproteinase-13 mRNA in the granulation tissue of equine superficial digital flexor tendinitis   总被引：1，自引：0，他引：1  

Nomura M  Hosaka Y  Kasashima Y  Ueda H  Takehana K  Kuwano A  Arai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):637-639

The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon. In situ hybridization also revealed that fibroblastic cells proliferated in the granulation tissue generated in the tendinitis actively expressed MMP-13 mRNA. On the other hand, in normal tendon, a few fibroblastic cells and vascular components lied in the endotenon barely expressed its mRNA, but other cellular components in the tendon bundle were not positively hybridized. As mentioned above, MMP-13 but not other collagenases or gelatinases, may play an important role in tendon injuries in the racehorses.  相似文献   

Effect of danazol on NK cells and cytokines in the mouse uterus     

Kusakabe K  Morishima S  Nakamuta N  Li ZL  Otsuki Y 《The Journal of reproduction and development》2007,53(1):87-94

Danazol, which has been used as a medicine for endometriosis, has a valid effect in pretreatment of patients receiving in vitro fertilization and embryo transfer, although its reproductive mechanism remains unclear. BALB/c mice were subcutaneously injected with danazol for 2 weeks. Blood and uteri were collected and cytokines were assayed. Following danazol treatment, an increase in pregnancy ratio was evident that was accompanied by up-regulation in serum macrophage-colony stimulating factor (M-CSF). RT-PCR analysis revealed that expression of M-CSF and Ly49, a phenotypic marker of natural killer (NK) cells, was up-regulated in the uteri of the danazol-treated mice. In immunohistochemical analysis, M-CSF and Ly49, together with alpha5 integrin, were clearly detected in the endometrium of the danazol-treated mice with very similar pattern of localization. These results suggest that danazol has an effect to promote pregnancy that induces recruitment of NK cells and a concomitant increase in the expression of M-CSF and alpha5 integrin in the uterus.  相似文献   

Growth properties and immunogenicity of a virus generated by reverse genetics for an inactivated equine influenza vaccine     

Minoru Ohta  Hiroshi Bannai  Yoshinori Kambayashi  Norihisa Tamura  Koji Tsujimura  Seiya Yamayoshi  Yoshihiro Kawaoka  Manabu Nemoto 《Equine veterinary journal》2022,54(1):139-144

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Nitrate leaching in granitic regosol as affected by N uptake and transpiration by corn     

Maho HASHIMOTO  Yoshinori HERAI  Toshinori NAGAOKA  Kenji KOUNO 《Soil Science and Plant Nutrition》2007,53(3):300-309

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Nutrient storage in termite (Macrotermes bellicosus) mounds and the implications for nutrient dynamics in a tropical savanna Ultisol     

Susumu S. Abe  Yoshinori Watanabe  Taisuke Onishi  Takashi Kotegawa  Toshiyuki Wakatsuki 《Soil Science and Plant Nutrition》2013,59(6):786-795

The role of mounds of the fungus-growing termite Macrotermes bellicosus (Smeathman) in nutrient recycling in a highly weathered and nutrient-depleted tropical red earth (Ultisol) of the Nigerian savanna was examined by measuring stored amounts of selected nutrients and estimating their rates of turnover via the mounds. A study plot (4?ha) with a representative termite population density (1.5?mounds?ha^?1) and size (3.7?±?0.4?m in height, 2.4?±?0.2?m in basal diameter) of M. bellicosus mounds was selected. The mounds were found to contain soil mass of 9249?±?2371?kg?ha^?1, composed of 7502?±?1934?kg?ha^?1 of mound wall and 1747?±?440?kg?ha^?1 of nest body. Significant nutrient enrichment, compared to the neighboring topmost soil (Ap1 horizon: 0–16?cm), was observed in the nest body for total nitrogen (N) and exchangeable calcium (Ca), magnesium (Mg) and potassium (K), and in the mound wall for exchangeable K only. In contrast, available (Bray-1) phosphorus (P) content was found to be lower in both the mound wall and the nest body than in the adjacent topmost soil horizon. Consequently, the mounds formed by M. bellicosus contained 1.71?±?0.62?kg?ha^?1 of total N, 0.004?±?0.003?kg?ha^?1 of available P, 3.23?±?0.81?kg?ha^?1 of exchangeable Ca, 1.11?±?0.22?kg?ha^?1 of exchangeable Mg and 0.79?±?0.21?kg?ha^?1 of exchangeable K. However, with the exception of exchangeable K (1.2%), these nutrients amounted to less than 0.5% of those found in the topmost soil horizon. The soil nutrient turnover rate via M. bellicosus mounds was indeed limited, being estimated at 1.72?kg?ha^?1 for organic carbon (C), 0.15?kg?ha^?1 for total N, 0.0004?kg?ha^?1 for available P, 0.15?kg?ha^?1 for exchangeable Ca, 0.05?kg?ha^?1 for exchangeable Mg, and 0.06?kg?ha^?1 for exchangeable K per annum. These findings suggest that the mounds of M. bellicosus, while being enriched with some nutrients to create hot spots of soil nutrients in the vicinity of the mounds, are not a significant reservoir of soil nutrients and are therefore of minor importance for nutrient cycling at the ecosystem scale in the tropical savanna.  相似文献   

Large-scale survey of frequency of forest walking and related factors in a Japanese population inhabiting a large city, and comparison of an urban area and a rural area     

Emi Morita  Kyoko Aoyama  Takashi Tamura  Rieko Okada  Sayo Kawai  Yoshinori Ito  Mariko Naito  Kenji Wakai  Nobuyuki Hamajima 《Journal of Forest Research》2013,18(6):454-461

The frequency of forest walking among the general population is a major topic of study in forest science. The objectives of this study were threefold: to assess the frequency of forest walking among Japanese residents of Nagoya, a large city; to evaluate differences among frequency of forest walking by participants from a large city, an urban area (Shizuoka; 4,666 participants), and a rural area (Yakumo Town, Hokkaido; 397 participants) in previous studies; and to examine factors related to frequency of forest walking. The survey, by self-administered questionnaire, in the major city of Nagoya was conducted between June 2008 and May 2010. In all, 5,158 participants (M/F, 1,466/3,692; mean age ± SD [range], 52.5 ± 10.3 [35–69] years) were included in the analysis. The proportions of frequency of forest walking ≥ once/month and ≥ once/year were 10.9 % (M/F, 15.1/9.3 %) and 46.1 % (51.0/44.1 %), respectively. In multiple logistic regression analysis, significant differences were noted among study sites for the adjusted odds ratio for frequency of forest walking. Overall, the order of highest to lowest frequency of forest walking was Shizuoka > Nagoya > Yakumo. Factors related to frequency of forest walking were common among the three study sites. Higher frequency of forest walking was associated with male sex and older age; the most relevant factor related to frequency of forest walking was its enjoyment level. Further studies will be required to clarify why these factors are related to frequency of forest walking.  相似文献   

Comparative analysis of the inverted repeat of a chalcone synthase pseudogene between yellow soybean and seed coat pigmented mutants     

Mineo Senda  Satsuki Nishimura  Atsushi Kasai  Setsuzo Yumoto  Yoshitake Takada  Yoshinori Tanaka  Shizen Ohnishi  Tomohisa Kuroda 《Breeding Science》2013,63(4):384-392

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