Characterization of a Lipoxygenase Gene in Rough Lemon Induced by Alternaria alternata     

Kenji GOMI  Hiroyuki YAMAMOTO  Kazuya AKIMITSU 《Journal of General Plant Pathology》2002,68(1):21-30

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Immunohistochemical distribution of inwardly rectifying K+ channels in the medulla oblongata of the rat     

Yamamoto Y  Ishikawa R  Omoe K  Yoshikawa N  Yamaguchi-Yamada M  Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):265-271

The inwardly rectifying K+ channels, Kir1.1, Kir2.3 and Kir4.1-Kir5.1, are the candidate chemosensory molecules for CO2/H+. We determined the mRNA expression and immunohistochemical localization of these channels in the medulla oblongata of the rat. RT-PCR analysis revealed mRNAs of Kir1.1, Kir2.3, Kir4.1 and Kir5.1 were detected in the medulla. The immunoreactivities for Kir1.1, Kir2.3, Kir4.1, and Kir5.1 were observed in the medulla, and immunolabeling pattern was varied by the subunit. Immunoreactivities for Kir1.1 and Kir2.3 were observed in the nerve cell bodies and glial cells both in the chemosensory areas [nucleus tractus solitarius (NTS), nucleus raphe obscurus (RO), pre-B?tzinger complex (PreB?tC)] and non-chemosensory area [hypoglossal nucleus (XII), inferior olive nucleus (IO)]. Kir4.1 immunoreactivity was observed in the glial cells and neuropil, especially in XII and IO. Kir5.1 immunoreactivity was observed in the nerve cell bodies in the XII, RO, and PreB?tC, but not in the NTS or IO. In the NTS, a dense network of varicose nerve fibers showed immunoreactivity for Kir5.1. Our findings suggest that Kir channels may not act specific to the central chemoreception, but regulate the ionic properties of cellular membranes in various neurons and glial cells.  相似文献   

Genetic characterization of chicken anemia viruses newly isolated from diseased chicks in Japan in 2020     

Hieu Van DONG  Giang Thi Huong TRAN  Aoi KUROKAWA  Yu YAMAMOTO  Yohei TAKEDA  Haruko OGAWA  Kunitoshi IMAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):166

In this study, a total of nine chicken samples obtained from two broiler flocks in Oita and Tottori prefectures in 2020 were examined for Chicken anemia virus (CAV) infection. The samples were collected from clinically suspected flocks and diseased chickens. The CAV genome was detected in all nine samples tested by real-time PCR. Phylogenetic analyses and sequence comparisons of the full-length VP1 gene sequences indicated that all the Japanese CAV strains obtained in this study formed a similar cluster of genotype III and shared high nucleotide (99.62–100%) identity. The current Japanese CAV strains were closely related to Chinese CAV strains but not related to vaccine strains. One positive selection site of VP1 was detected among the Japanese CAV strains.  相似文献   

Effect of drug-eluting bead transarterial chemoembolization loaded with cisplatin on normal dogs     

Ko NAKASUMI  Naoki YAMAMOTO  Taro TAKAMI  Harumichi ITOH  Kazuhito ITAMOTO  Hiro HORIKIRIZONO  Toshie ISERI  Munekazu NAKAICHI  Yuki NEMOTO  Hiroshi SUNAHARA  Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):114

Transcatheter arterial embolization (TAE) and transcatheter arterial chemoembolization (TACE) are standard treatments for advanced hepatocellular carcinoma (HCC) and particularly for unresectable tumors or liver metastases in humans. However, reports on TACE used in veterinary medicine are few. This study aimed to evaluate the feasibility and safety of drug-eluting bead transarterial chemoembolization (DEB-TACE). We performed DEB-TACE in four clinically normal dogs and pharmacokinetically compared the results against hepatic arterial infusion (HAI) of cisplatin in two dogs. Drug-eluting beads (DEB) loaded with cisplatin were injected through a microcatheter for selective embolization of the left hepatic artery. After embolization, computed tomography (CT) images and histological examination findings were obtained during a 4-week observation period. Serum platinum concentrations were measured to evaluate cisplatin after each procedure. Biochemical analysis was performed during a 12-week observation period. Embolization was successful in all dogs, and there were no clinically apparent abnormalities. Embolization was confirmed up to 4 weeks after DEB-TACE in two of the four dogs and up to 1 week in the other two dogs using postoperative CT images. Cisplatin was not detected in peripheral veins in all dogs after DEB-TACE, but it was detected in trace amounts after HAI. DEB-TACE using cisplatin was safe and well tolerated by normal dogs. DEB-TACE may be useful in terms of determining systemic toxicity and drug concentration within tumors.  相似文献   

Repeatability and minimum harvest period of cacao (Theobroma cacao L.) in Southern Bahia     

Luiz Antônio dos Santos Dias  Paulo Yoshio Kageyama 《Euphytica》1998,102(1):29-35

Classically, selection for superior genotypes in cacao has been based on the successive harvest records across a number of years. Little information on the minimum duration of these harvest periods is available in the literature. The repeatability coefficient (ρ) was used to estimate this period. Twenty five cacao genotypes were assayed in a randomized block design with four replications and 16-plant plots. The following yield components were studied: number of healthy fruits per plant, number of collected fruits per plant, weight of humid seeds per plant and per fruit, and percentage of diseased fruits per plant, over 5 years (1986–90). Repeatability estimates were higher than 0.84 for all components, except percentage of diseased fruits per plant (^ρ - 0.41). With such estimates, it is possible to select genotypes on the basis of only two years of successive harvests, with a determination coefficient of 90%. The advantages of applying the repeatability coefficient to the cacao breeding program are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Reduction of ferric chelate caused by various wood-rot fungi     

Hirofumi Hirai  Ryuichiro Kondo  Kokki Sakai  Yoshio Watanabe  Ryuichiro Kurane 《Journal of Wood Science》1999,45(3):262-265

The reduction of ferric chelate caused by various wood-rot fungi was analyzed. Ferric chelate reductive activity was detected in cell-free extracts of seven wood-rot fungi:Phanerochaete chrysosporium, P. sordida YK-624,Ganoderma sp. YK-505,Coriolus versicolor, Bjerkandera adusta, Tyromyces palustris, andGloeophyllum trabeum. These fungi produced NADPH- or NADH-dependent ferric chelate reductive enzymes (or both) of different molecular weight. In the liquid culture ofP. sordida YK-624 andC. versicolor, a positive correlation was observed between extracellular MnP activity and intracellular NADPH-dependent ferric chelate reductive activity.  相似文献   

Optimization of selection and mating schemes in closed broiler lines     

Tetsuro NOMURA  Shigeki YAMAGUCHI  Fumio MUKAI  Aya YAMAMOTO 《Animal Science Journal》2002,73(6):435-443

Minimum coancestry mating (MC) is a simple mating system to reduce inbreeding in populations, in which matings are allocated so as to minimize the average inbreeding coefficient of progeny. This system was compared with random mating (RM) in simulated broiler lines. The population structure and genetic parameters were determined on the basis of an existing broiler line. Comparison of mating systems was made under two selection methods. The first method (DIS) was based on selection index for achieving desired genetic gains. In the second method (LPS), a combination of the family index and linear programming technique was applied to obtain the desired genetic gains. The selected traits were body weight at 6 weeks of both sexes and age at sexual maturity of hen. Four schemes by all the possible combinations of selection and mating methods (DIS + RM, DIS + MC, LPS + RM and LPS + MC) were compared in terms of genetic gains and inbreeding during 15 generations of selection and mating. The results obtained are summarized as follows: (i) the four schemes produced similar genetic gains averaged over replicates; (ii) the variations of genetic gains under LPS + RM and LPS + MC schemes were much smaller than under DIS + RM and DIS + MC schemes; (iii) irrespective of the selection methods, MC reduced the average inbreeding coefficients to about 80% of RM and; (iv) the inbreeding coefficients of individuals in the schemes with RM were distributed in a wide range, while the inbreeding coefficients in the schemes with MC showed a high uniformity. From these results, the LPS + MC scheme was recommended as a selection and mating strategy in closed broiler lines.  相似文献   

Identification of Vibrio halioticoli using 16S rDNA PCR/RFLP (restriction fragment length polymorphism) analysis     

Reiji Tanaka  Tomoo Sawabe  Kenichi Tajima  Johan Vandenberghe  Yoshio Ezura 《Fisheries Science》2001,67(1):185-187

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Studies on Staphylococcosis in Chickens: I. Outbreaks of Staphylococcal Infection on Poultry Farms and Characteristics of Staphylococcus aureus Isolated from Chickens     

Shigemi Kuramasu  Yasuhisa Imamura  Takayasu Takizawa  Fusako Oguchi  Yoshio Tajima 《Zoonoses and public health》1967,14(7):646-656

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A preliminary report of efficiency of various mediums for isolation of pleuropneumonia-like organisms from exudates of birds with chronic respiratory disease     

ADLER HE  YAMAMOTO R  BANKOWSKI RA 《American journal of veterinary research》1954,15(56):463-465

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